scholarly journals Enhanced Biogas and Biofertilizer Production from Anaerobic Codigestion of Harvest Residues and Goat Manure

2020 ◽  
pp. 1-13
Author(s):  
Uduak U. Ndubuisi-Nnaji ◽  
Utibe A. Ofon ◽  
Naomi U. Asamudo ◽  
Victoria M. Ekong
Keyword(s):  
Rice Straw ◽  
Production Efficiency ◽  
Fold Increase ◽  
Plant Growth Promoting Bacteria ◽  
Bacterial Populations ◽  
Harvest Residues ◽  
Biogas Yield ◽  
Plant Growth Promoting ◽  
Goat Manure ◽  
Phosphate Solubilizing

Comparative assays were conducted to assess the biogas and biofertilizer production efficiency from anaerobically codigested goat manure (GM) and harvest residues: corn stover (CS) and rice straw (RS). All digesters were operated simultaneously under mesophilic temperature of 40°C and notable phosphate solubilizing and nitrogen fixing bacterial populations indicated qualitative biofertilizer quality of the digestates. Codigestion of the substrates significantly increased biogas yield (p < 0.05) compared to monodigestion, and the highest cumulative yield of 573 ml/g VS was obtained from codigested rice straw (RS) and goat manure (GM). With a significant decimation in number of pathogens (p < 0.05), a 2 – 3 fold increase in populations of plant growth promoting bacteria (Bacillus and Pseudomonas species) was observed in digestate from codigestion assays when compared to monodigestion (control) and were identified as Clostridium sp., Bacillus subtilis, Bacillus megaterium, Lactobacillus sp., Pseudomonas fluorescens including methanogens: Methanothrix sp., Methanobacterium sp. and Methanosarcina sp. On the average, codigestion assays resulted in enhanced biogas yield and biofertilizer quality that was 2.3 – 4.1 times higher than single substrate digestion and generally improved the efficiency of biogas and biofertilizer production.

Community dynamics of duckweed-associated bacteria upon inoculation of plant growth-promoting bacteria

2020 ◽  
Vol 96 (7) ◽  
Author(s):  
Hidehiro Ishizawa ◽  
Masashi Kuroda ◽  
Daisuke Inoue ◽  
Masaaki Morikawa ◽  
Michihiko Ike
Keyword(s):  
Plant Growth ◽  
Bacterial Communities ◽  
Production Efficiency ◽  
Community Dynamics ◽  
Amplicon Sequencing ◽  
Plant Colonization ◽  
Plant Growth Promoting Bacteria ◽  
Short Period ◽  
Plant Growth Promoting ◽  
Growth Promoting

ABSTRACT Plant growth-promoting bacteria (PGPB) have recently been demonstrated as a promising agent to improve wastewater treatment and biomass production efficiency of duckweed hydrocultures. With a view to their reliable use in aqueous environments, this study analysed the plant colonization dynamics of PGPB and the ecological consequences for the entire duckweed-associated bacterial community. A PGPB strain, Aquitalea magnusonii H3, was inoculated to duckweed at different cell densities or timings in the presence of three environmental bacterial communities. The results showed that strain H3 improved duckweed growth by 11.7–32.1% in five out of nine experiments. Quantitative-PCR and amplicon sequencing analyses showed that strain H3 successfully colonized duckweed after 1 and 3 d of inoculation in all cultivation tests. However, it significantly decreased in number after 7 d, and similar bacterial communities were observed on duckweed regardless of H3 inoculation. Predicted metagenome analysis suggested that genes related to bacterial chemotactic motility and surface attachment systems are consistently enriched through community assembly on duckweed. Taken together, strain H3 dominantly colonized duckweed for a short period and improved duckweed growth. However, the inoculation of the PGPB did not have a lasting impact due to the strong resilience of the natural duckweed microbiome.

scholarly journals Mineral Phosphate Solubilizing Bacteria Isolated from Various Plant Rhizosphere under Different Aluminum Content

2015 ◽  
Vol 10 (2) ◽  
Author(s):  
Dolly Iriani Damarjaya ◽  
Jaka Widada ◽  
Keishi Senoo ◽  
Masaya Nishiyama ◽  
Shigeto Otsuka
Keyword(s):  
Dry Weight ◽  
Rrna Gene ◽  
Plant Growth Promoting Bacteria ◽  
Phosphate Solubilizing Bacteria ◽  
Gene Sequence Analysis ◽  
Mineral Phosphate ◽  
Plant Growth Promoting ◽  
The Media ◽  
Formation Method ◽  
Phosphate Solubilizing

The objectives of this study was to isolate and characterize the mineral phosphate solubilizing bacteriafrom rhizosphere and evaluate their potential as plant growth promoting bacteria in Al-toxic soils. The halozone formation method was used to isolate PSB using the media containing insoluble phosphates (Ca-P or Al-P)as a source of phosphate. Eight of acid and Al-tolerant PSB isolates that were able to solubilize Ca-P wereobtained from rhizosphere of clover, wheat, corn, and sunflower grown in Al-toxic soil. Identification of theisolates based on the 16S rRNA gene sequence analysis demonstrated that the isolates were strains of Burkholderia(5 strains), Pseudomonas (1 strain), Ralstonia (1 strain), and unidentified bacterium (1 strains). All PSB isolatesshowed the capability to dissolve Ca-P, and only 1 strain (Ralstonia strain) was able to dissolve Al-P in agar platemedium. The P-solubilization by these isolates was correlated with pH of medium. Inoculation of the bacterialstrains on clover on Al-toxic medium showed that all isolates increased the plant dry weight compared withuninoculated treatment. Our results showed that those PSB isolates have potential to be developed as a biofertilizerto increase the efficiency of P-inorganic fertilizer used in Al-toxic soils.

Phosphate Solubilization and Plant Growth Promoting Actinobacteria from Rhizosphere Soil

2020 ◽  
Author(s):  
K. Balakrishnan ◽  
J. Thirumalairaj ◽  
M. Radhakrishnan ◽  
V. Gopikrishnan ◽  
R. Balagurunathan
Keyword(s):  
Plant Growth ◽  
Phosphatase Activity ◽  
Rhizosphere Soil ◽  
Phosphate Solubilization ◽  
Control Plant ◽  
Agar Plate ◽  
Plant Growth Promoting Bacteria ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Phosphate Solubilizing

Background: Phosphorus has been considered as the key element as it is directly involved in most of the life processes including in plants. Soil contains both organic and inorganic forms of phosphorus. Phosphate solubilization is the important property of soil bacteria to develop them as plant growth promoting bacteria in the agricultural field. The present study report the phosphate solubilizing and plant growth promoting properties of selected actinobacteria isolated from rhizosphere soil.Methods: Actinobacterial strains were isolated from rhizosphere soil and screened for in vitro phosphate solubilizing properties using agar plate method. The efficiency of phosphate solubilization and phosphatase activity of isolated actinobacterial strains were tested using Pikovskaya broth. Result: In the present study phosphate solubilization and phosphatase activity of isolated actinobacteria, Streptomyces sp. CTD-2 was comparatively higher in lab conditions. In pot trial experiment strain CTD-2 showed higher growth when compared to the control plant. The chlorophyll content of leaves in the experimental plants were found maximum with actinobacteria strain CTD-2 treated plant. Plant growth measurements such as root length, shoot length, leaf length, total plant growth measurements, quick yield production were also observed. 

scholarly journals Establishing and Quantifying the Phosphate Solubilizing Potential of the Brinjal Bacterial Isolate

2021 ◽  
Vol 10 (11) ◽  
pp. 83-88
Author(s):  
C. Jenifer Lolita A. C. Manjula ◽  
E. Keshamma
Keyword(s):  
Plant Growth ◽  
Bacterial Isolate ◽  
Nitrogenase Activity ◽  
Solanum Melongena ◽  
Steady Increase ◽  
Plant Growth Promoting Bacteria ◽  
Phosphate Solubilizing Bacteria ◽  
Beneficial Effects ◽  
Plant Growth Promoting ◽  
Phosphate Solubilizing

Rhizosphere, phylloplane and caulosphere is the region where a complex community of microbes, mainly bacteria and fungi are present. The microbe plant interaction in these regions can be beneficial, neutral, variable, or deleterious for plant growth. The bacteria that exert beneficial effects on plant development are termed plant growth promoting bacteria. To quantify the amount of phosphate solubilizing bacteria from rhizosphere, phylloplane and caulosphere of brinjal (Solanum melongena L.). Materials and methods: Brinjal (Solanum melongena L.) plants of different varieties were collected from seven locations around Bangalore viz., Hessaraghatta, Yelahanka, Kengeri, Madi vala, Hebbal, Tirumalapura and Attibele were also screened for the presence of phosphate solubilizing bacteria. Nitrogenase activity was estimated by acetylene reduction assay and analyzed by gas chromatography. The amount of nitrogen fixed brinjal bacterial isolate was quantified by micro Kjeldhal method. The amount of nitrogen fixed by the BBI was equivalent to 23.5 nm of C2H2 reduced/tube/hour. The amount of nitrogen fixed by the BBI showed a steady increase upto three days (75 nm of C2H2 reduced/tube/hour) after which there was a decline in the amount of nitrogen fixed by the microbe. Phosphate solubilization by the bacteria isolated from brinjal is highly beneficial to the crop, as it would always make more phosphate available to the crop. This phosphate solubilizing potential could be harnessed to reduce the input of inorganic fertilizers. For the first time the presence of phosphate solubilizing bacteria on the rhizosphere and endorhizosphere of brinjal (Solanum Melongena L.) cultivars was established.

scholarly journals Characterisation of bacterial diversity in fresh and aged sewage sludge biosolids using next generation sequencing.

Detritus ◽  
2020 ◽  
pp. 82-91
Author(s):  
Karen Ruth Little ◽  
Han Ming Gan ◽  
Aravind Surapaneni ◽  
Jonathan Schmidt ◽  
Antonio Frank Patti
Keyword(s):  
Sewage Sludge ◽  
Plant Growth ◽  
Wastewater Treatment Plants ◽  
Fold Increase ◽  
Land Application ◽  
Plant Growth Promoting Bacteria ◽  
Potential Source ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Generation Sequencing

Sewage sludge, often referred to as biosolids, is generated in large quantities by wastewater treatment plants. It contains macro- and micronutrients which are essential for plant growth and so represents a valuable agricultural resource. Prior to land application, pathogens are carefully monitored to reduce the risk of crop and soil contamination however to date there has been limited investigation of agriculturally beneficial bacteria indigenous to the biosolids. This study investigated shifts in the composition of the bacterial community alongside the physicochemical properties of biosolids of increasing age, from freshly dewatered to those stockpiled for approximately four years. With stockpiling, there was a significant increase in ammonium content, ranging from 801 mg/kg in the fresh biosolids to 8178 mg/kg in the stockpiled biosolids and a corresponding increase in pH ranging from 6.93 to 8.21. We detected a ten-fold increase in Firmicutes, from 4% relative abundance in the fresh biosolids compared to 40% in the older, stockpiled biosolids. Plant growth promoting bacteria (PGPB) of the Proteobacteria family, particularly of the Devosia and Bradyrhizobium genera were identified in the freshly dewatered and the older, stockpiled biosolids. Land application of the biosolids studied here could reduce fertiliser costs, provide a means of pH correction to acidic soils and a potential source of bacteria beneficial for crop growth.

scholarly journals Vermicompost Assisted Phytoremediation of Heavy Metal Contaminated Soil in Madaka District, Nigeria Using Melissa officinalis L (Lemon balm) and Sida acuta (Stubborn weed)

2021 ◽  
Author(s):  
sesan abiodun aransiola ◽  
Udeme Joshua Joshia Ijah ◽  
Olabisi Peter Abioye ◽  
Jeremiah David Bala
Keyword(s):  
Heavy Metals ◽  
Structural Changes ◽  
Translocation Factor ◽  
Plant Growth Promoting Bacteria ◽  
Accumulation Coefficient ◽  
Sida Acuta ◽  
Plant Parts ◽  
Plant Growth Promoting ◽  
Goat Manure ◽  
Assisted Phytoremediation

Abstract This research was designed to clean-up through an environmentally friendly techniques, a polluted environment of Madaka District of Shikira. Physicochemical properties of the soil were done using standard methods. Chicken dropping vermicompost (CDV) and goat manure vermicompost (GMV) were produced by standard method to assist the phytoremediation process with plant growth promoting bacteria (PGPB). Canonical discriminant functions of the heavy metals were done. For the first location (AK) the plant parts of M. officinalis L mopped up heavy metals, the concentration of Cd, As, Pb in plant parts varied from 0.007 to 0.33 mg/kg, As from 0.09 to 4.39 mg/ kg and Pb from 0.07 to 10.35 mg/kg respectively while the concentration of heavy metals in S. acuta parts had Cd, As, Pb varied from 0.002 to 0.43 mg/kg, As from 0.27 to 3.79 mg/ kg and Pb from 1.68 to 10.7 mg/kg respectively. The second location (AM) also had the two plants mopping up heavy metals at different concentrations. the concentration of Cd, As, Pb in M. officinalis L parts varied from 0.03 to 0.41 mg/kg, As from 0.65 to 4.65 mg/ kg and Pb from 1.93 to 11.49 mg/kg respectively while the concentration of heavy metals in S. acuta parts had Cd, As, Pb varied from 0.06 to 0.66 mg/kg, As from 0.68 to 4.64 mg/ kg and Pb from 1.53 to 11.53 mg/kg respectively. Melissa offinalis L and Sida acuta were found most suitable for phytoextraction of sites contaminated with Cd, As and Pb because both of them have their bioconcentration factor (BCF), translocation factor (TF) and biological accumulation coefficient (BAC) to be ˃1 while both were also scored as phytostabilizer because they have BCF ˃1 and TF ˂1. Soil structural changes pre and post remediation were determined through x-ray fluorescence (XRF spectroscopy) and scanning electron microscopy (SEM) analyses.

scholarly journals Survival of Microbial consortium in granular formulations, degradation and release of microorganisms in soil

2016 ◽  
Vol 5 (05) ◽  
pp. 1348 ◽  
Author(s):  
Swapna G.* ◽  
Divya M. ◽  
Brahmaprakash G.P.
Keyword(s):  
Finger Millet ◽  
Microbial Consortium ◽  
Green Revolution ◽  
Plant Growth Promoting Bacteria ◽  
Phosphate Solubilizing Bacteria ◽  
Agriculture Sustainability ◽  
Plant Growth Promoting ◽  
Food Grain ◽  
Major Nutrients ◽  
Phosphate Solubilizing

The green revolution bought amazing consequences in food grain production but with insufficient concern for agriculture sustainability. Biofertilizers are gaining importance in sustaining agriculture. Various complementing combinations of microbial inoculants for management of major nutrients are necessary for agriculture sustainability. The present investigation was conducted to study the survivability of granular formulations containing Nitrogen fixing bacteria (Azotobacter chrococcum), phosphate solubilizing bacteria (Bacillus megaterium) and plant growth promoting bacteria (Pseudomonas fluorescens) in consortium prepared using different flour. Maximum survival of microbial consortium was observed in Soybean, followed by Soybean + Semolina and Rice inoculant formulations. Minimum survival of population was observed in Ragi + Semolina inoculant formulation. Wheat, wheat + semolina, soybean, soybean + semolina granular formulations have shown better degradation compared to other granular formulations both in presence and absence of tomato and finger millet (Eleusine coracana). Among different granular inoculant formulations, Maximum release was observed in soybean granular inoculant formulations in presence of tomato and Finger millet plant and in absence of plant and minimum release of microbial consortium was observed in ragi + semolina granular inoculant formulations during incubation in soil.

Screening of Azotobacter strains from rice plant rhizosphere for phosphate solubilizing properties

2021 ◽  
Vol 27 (2) ◽  
Author(s):  
Niharika Dewangan ◽  
Shirin Anwar
Keyword(s):  
Plant Growth ◽  
Rice Plant ◽  
Phosphate Solubilization ◽  
Plant Growth Promoting Bacteria ◽  
Crop Fields ◽  
Rice Rhizosphere ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Phosphate Solubilizing ◽  
Phosphate Solubilizer

Azotobacter is a plant growth promoting bacteria which solubilize phosphorous and make it available for plants. In the present study the efforts have been taken to fulfill the aim to isolate, characterize and screen Azotobacter for phosphate solubilization from the rice rhizosphere of Durg district. Total 8 strains of Azotobacter were isolated from different rice fields. It was observed that all the strains were found to be phosphate solubilizing but in different quantity. RAZB6 is highest phosphate solubilizer (234.4±1mg/L) and RAZB4 shows minimum (82.09±1mg/L) quantity of phosphate solubilization. Since phosphorous is necessary for the plants proper development and it increases the growth of rice plant and make soil more fertile for other plants growth also so they must be commercialized and used more and more in the crop fields other than the agrochemicals.

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