scholarly journals Effect of extraction method on the antioxidative activity of ground elder (Aegopodium podagraria L.)

2019 ◽  
Vol 21 (3) ◽  
pp. 13-18 ◽  
Author(s):  
Agnieszka Wróblewska ◽  
Katarzyna Janda ◽  
Edyta Makuch ◽  
Marika Walasek ◽  
Piotr Miądlicki ◽  
...  
Keyword(s):  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Extraction Methods ◽  
Extraction Time ◽  
Lower Amount ◽  
Antioxidative Properties ◽  
Assisted Extraction ◽  
Soxhlet Apparatus ◽  
Ultrasonic Assisted ◽  
Method Of Extraction

Abstract In this work the studies on the antioxidative properties of extracts from various morphotic parts of the ground elder (leaves, rhizomes, seeds and flowers) were presented. Moreover, the effect of different extraction methods (ultrasonic assisted extractions, extraction in a Soxhlet apparatus, extraction at the boiling point of the solvent used), solvent and its amount, and extraction time on the antioxidative properties of the obtained extracts were tested. The studies showed that all parts of ground elder can show radical scavenging activity, and it depends mainly on the method of extraction and extraction time. But the most beneficial is ultrasonic assisted extraction which used lower amount of solvent (ethanol). In case of all parts of the ground elder (leaves, rhizomes, seeds and flowers) it allows to obtain very high values of the antioxidant capacity (above 90%) for very short extraction time amounted to 20–40 minutes.

scholarly journals Achievement of antioxidants from Abelmoschus esculentus L. seeds using ultrasound-assisted extraction optimized by the help of experimental design approach

2020 ◽  
Vol 14 (2) ◽  
pp. 160-168
Author(s):  
Merve Bat Özmatara ◽  
Şule Dinç Zor
Keyword(s):  
Experimental Design ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Extraction Methods ◽  
Extraction Time ◽  
Abelmoschus Esculentus ◽  
Scavenging Activity ◽  
Ultrasound Assisted Extraction ◽  
Extraction Solvent ◽  
Assisted Extraction

Developments of food supplements and nutraceuticals from different natural origins require the use of some extraction methods. The success of the procedure relies on theefficiency of the extraction methods. Therefore, extraction methods need to be optimized. This work aimed for the determination of optimal conditions of ultrasonic-assisted extraction (UAE) of antioxidant constituents from okra (Abelmoschus esculentus L.) seeds which contain high contents of polysaccharides, polyphenols and flavonoids using response-surface methodology based on Box-Behnken experimental design (BBD). The independent variables of UAE were extraction time (Et, 10-20 min), extraction solvent composition (EC, 50-100% methanol) and extraction solvent volume (EV, 10-20 mL). 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging activity, 2,2'-azinobis- (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical-scavenging activity and N,N-dimethyl-p-phenylenediamine dihydrochloride (DMPD) radical-scavenging activity were dependent variables. Optimal extraction conditions for UAE from okra seed were: 20 mL volume of 70% methanol at 10 min extraction time for DPPH inhibition; 20 mL volume of 50% methanol at 20 min for ABTS inhibition; 15 mL volume of 55% methanol at 12 min for DMPD inhibition. Under these optimum extraction conditions, the effectiveness of UAE of okra seed was successfully revealed. So, this practical extraction technique can be widely used for the achievement of dietary supplements and nutraceuticals.

scholarly journals Comparison of Classical and Ultrasound-Assisted Extraction Methods on Antioxidant Activities of Pharmaceutically Active Compounds from Valeriana Officinalis

2021 ◽  
Vol 5 (2) ◽  
Keyword(s):  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Extraction Methods ◽  
Total Phenolic ◽  
Valeriana Officinalis ◽  
Assisted Extraction ◽  
Ultrasonic Assisted ◽  
Dpph Radical Scavenging

Medicinal plants are good sources and rich of substances which having nutraceutical and health benefits. Valeriana officinalis L. (Valerianaceae) is a known medicinal plant used in traditional medicine worldwide. The aim of present study was to evaluate the efficiencies of three methods for evaluated for antioxidant from Valeriana officinalis. Besides, the impact of extraction methods on total phenollic and flavonoids contents and antioxidant activities of Valeriana root was investigated. The dried-root was extracted by three different methods including maceration, ultrasonic assisted, and Soxhlet assisted extraction. Antioxidant capacity was assessed using four different methods: DPPH and nitric oxide (NO) free radicals scavenging, reducing power and iron chelating activity. The total phenolic and flavonoid contents were also identified. The ultrasonic extract showed highest amount of total phenolics and flavonoids contents. In DPPH radical scavenging activity, the ultrasonic assisted extract, (IC50=0.546 μg/ ml) had a higher activity from other extracts. In reducing power assay, maceration extract showed the highest activity. In DPPH radical scavenging activity, IC50 for ultrasonic extract, soxhlet assisted extraction and maceration extract were 0.546, 0.816 and 0.678 μg/ml, respectively. Here, the maceratin extract showed the highest activity, too. The results clearly indicated the extraction methods used in this study significantly affected antioxidant capacities and total phenolic and flavonoids contents. Ultrasonic assisted extraction and Soxhlet methods were found to be more efficient in extraction of antioxidant components of valeriana.

scholarly journals Ultrasonic-Assisted Extraction of Natural Yellow Pigment from Physalis pubescens L. and Its Antioxidant Activities

2018 ◽  
Vol 2018 ◽  
pp. 1-6
Author(s):  
Haitang Wang ◽  
Lin Shi ◽  
Xiaoyu Yang ◽  
Rui Hong ◽  
Liang Li
Keyword(s):  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Yellow Pigment ◽  
Ultrasonic Power ◽  
Optimal Parameters ◽  
Assisted Extraction ◽  
Ultrasonic Time ◽  
Ultrasonic Assisted ◽  
Ultrasonic Assisted Extraction

Physalis pubescens L. is rich in natural pigments but has not yet been fully utilized. Ultrasound-assisted extraction of yellow pigment from Physalis pubescens L. was investigated by response surface methodology in this study. Optimal parameters were ultrasonic power of 29.21%, ultrasonic time of 14.41 min, and ultrasonic interval time of 10.55 s. The yield was 0.193% under optimal parameters. FRAP, ABTS, and superoxide radical scavenging activity of the yellow pigment were 6.11 ± 0.22 mmol/g, 2.80 ± 0.27 mmol/g, and 57281.5 ± 2749.5 U/g, respectively. The results showed that the yield of yellow pigment could be improved by ultrasonic-assisted extraction and the yellow pigment extracted by ultrasound had antioxidant activity.

scholarly journals Simultaneous Optimization for Ultrasound-Assisted Extraction and Antioxidant Activity of Flavonoids from Sophora flavescens Using Response Surface Methodology

Molecules ◽  
2018 ◽  
Vol 24 (1) ◽  
pp. 112 ◽  
Author(s):  
Jing Zhou ◽  
Lincheng Zhang ◽  
Qinping Li ◽  
Weifeng Jin ◽  
Weiyan Chen ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Response Surface Methodology ◽  
Response Surface ◽  
Radical Scavenging ◽  
Methanol Concentration ◽  
Extraction Time ◽  
Sophora Flavescens ◽  
Assisted Extraction ◽  
Ultrasonic Assisted ◽  
Ultrasonic Assisted Extraction

The ultrasonic-assisted extraction process and antioxidant activity of flavonoids from Sophora flavescens were investigated in this study. In order to optimize the extraction of flavonoids from Sophora flavescens, the influence of extraction time, methanol concentration, ultrasonic temperature, and solvent-to-material ratio was analyzed. Results showed that the extraction yields reached a maximum with the extraction time of 30 min, methanol concentration of 80%, temperature of 80 °C, and solvent-to-material ratio of 26 mL/g. The flavonoids were determined by HPLC, and the mean yields of trifolirhizin, formononetin, isoxanthohumol, maackiain, and kurarinone under the optimal conditions were 2.570, 0.213, 0.534, 0.797, and 3.091 mg/g, respectively. The evaluation of vitro antioxidant activity exhibited Sophora flavescens flavonoids had a strong 1,1-diphenyl-2-picrylhydrazyl (DPPH) and hydroxyl radical-scavenging ability with IC50 of 0.984 and 1.084 mg/g, respectively. These results indicate that ultrasonic-assisted extraction is an efficient approach for the selective extraction of flavonoids, and response surface methodology further optimized the extraction.

OPTIMIZATION OF ULTRASONIC ASSISTED EXTRACTION OF ANTIOXIDANTS FROM MORINGA OLEIFERA LAM. LEAVES USING RESPONSE SURFACE METHODOLOGY

INDIAN DRUGS ◽  
2019 ◽  
Vol 56 (12) ◽  
pp. 32-38
Author(s):  
H. N Sholapur ◽  
◽  
F. S Dasankoppa ◽  
Z. Abbas ◽  
N.G.N. Swamy
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Moringa Oleifera ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Scavenging Activity ◽  
Solid Ratio ◽  
Assisted Extraction ◽  
Ultrasonic Assisted ◽  
Ultrasonic Assisted Extraction

The present study was embarked upon for the optimization of conditions aimed at ultrasonic assisted extraction (UAE) of antioxidant chemicals from Moringa oleifera leaves by applying response surface methodology (RSM) based on face centered central composite design (CCD). Evaluation was done at three levels (20 experimental designs) with three process variables: extraction temperature (20, 30, 40oC), extraction time (20, 30, 40 min) and liquid-solid ratio (20:1, 30:1, 40:1) using probe sonicator. The extraction process optimization based on RSM was focused on the capacity of antioxidants present in the extracts to scavenge the 2,2-diphenyl-1-picrylhydrazyl radical (DPPH) free radicals. To envisage the optimization process, multiple regression analysis was carried out using RSM tool to obtain a quadratic polynomial equation. Stastical analysis of the factors and their interactions were tested using analysis of variance (ANOVA). Extraction of antioxidants was mainly influenced by liquid-solid ratio and temperature. The ideal combination of variables designed by RSM for maximum radical-scavenging activity was optimized with extracting temperature of 39.80 °C, extracting time of 39.95 min and liquidsolid ratio of 24.00 mL/g to obtain a predicted value of 85.528% of DPPH radical-scavenging activity, which was very close to the experimentally value of 86.49 % with an error of 1.12%, indicating suitability of the model.

scholarly journals Quality of extracts from blueberry pomace by high hydrostatic pressure, ultrasonic, microwave and heating extraction：A comparison study

2017 ◽  
pp. 815 ◽  
Author(s):  
Haining Zhang ◽  
William Tchabo ◽  
Yongkun Ma
Keyword(s):  
Hydrostatic Pressure ◽  
High Hydrostatic Pressure ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Extraction Methods ◽  
Extraction Yield ◽  
Heat Extraction ◽  
Total Phenols ◽  
Assisted Extraction ◽  
Significant Difference

The objective of this study was to compare the efficiency of high hydrostatic pressure, ultrasonic, microwave, and heat extraction techniques on the blueberry pomace extract characteristics. The results showed that the highest extraction yield of anthocyanins and total phenols were obtained by high hydrostatic pressure assisted extraction. The difference of extraction yield of anthocyanins was significant (p<0.05) between high hydrostatic pressure assisted extraction and heating extraction. Furthermore, significant difference of total phenols extraction yield was obtained between high hydrostatic pressure assisted extraction and the other three extraction methods. The total color difference was significant for the four extraction methods. High hydrostatic pressure assisted extraction had the highest chroma and least browning index. Moreover, high hydrostatic pressure assisted extraction was found to have the highest hydroxyl radical scavenging activity. In summary, high hydrostatic pressure assisted extraction was revealed to be the suitable technique for phenolic extraction of blueberry pomace.

scholarly journals OPTIMIZATION SEQUENTIAL MICROWAVE ULTRASONIC ASSISTED EXTRACTION TO EXTRACT POLYPHENOL FROM MORINGA OLEIFERA LEAVES USING MULTIRESPONSE SURFACE METHODOLOGY

2021 ◽  
Vol 9 (5) ◽  
pp. 444-451
Author(s):  
G. Restu Prinanda ◽  
◽  
Aji Prasetyaningrum ◽  
Wahyudi a ◽  
Dwi Purwati ◽  
...  
Keyword(s):  
Moringa Oleifera ◽  
Extraction Process ◽  
Extraction Methods ◽  
Extraction Time ◽  
Total Phenolic ◽  
Assisted Extraction ◽  
Ultrasonic Assisted ◽  
Moringa Oleifera Leaves ◽  
Speed Up ◽  
Ultrasonic Assisted Extraction

Moringa leaves contain phenolic compounds and flavonoids which are useful as antioxidants. The disadvantages of traditional extraction methods, such as maceration, include a lengthy extraction process and the use of excessive solvent. Therefore, in this study, moringa leaf extraction was carried out using sequential microwave ultrasonic-assisted extraction (MUAE) to speed up the extraction time, and get more yields. This study aimed to improve the extraction of Moringa leaves with MUAE by adjusting the extraction time, temperature, and ethanol percentage. The conditions varied at extraction time (0, 10, 15, 20, 30 minutes), temperature (30, 40, 50, 60, 80 oC), and ethanol concentration (0, 30, 50, 70, 90%). The findings revealed that the best conditions for extraction Moringa leaves with sequential microwave-ultrasonic assisted extraction were at a time of 15 minutes, a temperature of 55oC, and an ethanol percentage of 54%. In this condition, the extract yield was 12.95%, total phenolic 330.38 GAE mg / 100 g, flavonoids in total 298.15 QE mg / 100 g, the IC50for antioxidant activity is 78.37 ppm.

scholarly journals Ultrasound-assisted extraction of protein from Bombay locusts and its impact on functional and antioxidative properties

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Passakorn Kingwascharapong ◽  
Manat Chaijan ◽  
Supatra Karnjanapratum
Keyword(s):  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Surface Hydrophobicity ◽  
Molecular Characteristics ◽  
Ultrasound Assisted Extraction ◽  
Antioxidative Properties ◽  
Assisted Extraction ◽  
Ultrasound Assisted ◽  
Nonessential Amino Acids ◽  
The Impact

AbstractImpact of ultrasound-assisted process (UAP) on yield, functional properties, antioxidant properties and molecular characteristics of protein extracted from Bombay locusts (BL) (Patanga succinta L.) was studied. Different conditions of UAP were implemented for different amplitudes (40–60%) and times (10–30 min) during aqueous extraction. Notably, UAP could enhance yield and protein recovery, compared with those from typical process (TP) (continuously stirred at 100 rpm at room temperature for 1 h). UAP conditions used governed the change of surface hydrophobicity and free α-amino content of BL. UAP could improve solubility of BL, especially at pH levels higher than 2. UAP had no significant (p > 0.05) detrimental effects on foaming capacity and stability of BL. Nevertheless, UAP, particularly at 50–60% amplitudes, affected the emulsion activity and stability of BL. UAP provided BL with high radical scavenging activities and good electron donating ability, especially that from 60% amplitude for 20 min (UAP-60/20). UAP-60/20 showed the impact on change of isoelectric point and molecular characteristic monitored by Fourier transform infrared (FTIR) of BL, compared to those from TP. In addition, BL was also an excellent source of both essential and nonessential amino acids. Therefore, UAP potentially enhanced BL extraction efficiency, resulting the BL with good functional and antioxidative properties.

scholarly journals Changes in Amino Acid Profiles and Bioactive Compounds of Thai Silk Cocoons as Affected by Water Extraction

Molecules ◽  
2021 ◽  
Vol 26 (7) ◽  
pp. 2033
Author(s):  
Chuleeporn Bungthong ◽  
Sirithon Siriamornpun
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Biological Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Silk Protein ◽  
Extraction Time ◽  
Antioxidant Power ◽  
Silk Cocoon ◽  
Amino Acid Profiles

Silk proteins have many advantageous components including proteins and pigments. The proteins—sericin and fibroin—have been widely studied for medical applications due to their good physiochemical properties and biological activities. Various strains of cocoon display different compositions such as amino-acid profiles and levels of antioxidant activity. Therefore, the objectives of this study were to find a suitable silk protein extraction method to obtain products with chemical and biological properties suitable as functional foods in two strains of Bombyx mori silk cocoon (Nangsew strains; yellow cocoon) and Samia ricini silk cocoon (Eri strains; white cocoon) extracted by water at 100 °C for 2, 4, 6 and 8 h. The results showed that Nangsew strains extracted for 6 h contained the highest amounts of protein, amino acids, total phenolics (TPC) and total flavonoids (TFC), plus DPPH radical-scavenging activity, ABTS radical scavenging capacity, and ferric reducing antioxidant power (FRAP), anti-glycation, α-amylase and α-glucosidase inhibition. The longer extraction time produced higher concentrations of amino acids, contributing to sweet and umami tastes in both silk strains. It seemed that the bitterness decreased as the extraction time increased, resulting in improvements in the sweetness and umami of silk-protein extracts.

Optimization of Ultrasonic-Assisted Extraction of Polysaccharides from Ottelia acuminata (Gagnep.) Dandy Using RSM

2011 ◽  
Vol 396-398 ◽  
pp. 292-296
Author(s):  
Ai Shi Zhu
Keyword(s):  
Processing Parameters ◽  
Extraction Time ◽  
Extraction Temperature ◽  
Independent Variables ◽  
Solid Ratio ◽  
Assisted Extraction ◽  
Ultrasonic Technology ◽  
Ultrasonic Assisted ◽  
Experimental Yield ◽  
Ultrasonic Assisted Extraction

Ultrasonic technology was applied to polysaccharides extraction from Ottelia acuminata (Gagnep.) Dandy and Response Surface Methodology (RSM) was used to optimize the effects of processing parameters on polysaccharides yields. Three independent variables such as liquid-solid ratio (ml/g, X1), extraction temperature (°C, X2) and extraction time (hour, X3) were investigated respectively. The statistical analysis indicated that the three variables and the quadratic of X1 and X3 had significant effects on the yields and followed by the significant interaction effects between the variables of X1 and X3, X2 and X3 (p<0.05). A mathematical model with high determination coefficient was gained. The optimal extraction conditions of polysaccharides were determined as follows: liquid-solid ratio 43 ml/g, extraction temperature 90 °C and extraction time 3.45 hours. Under these conditions, the experimental yield of polysaccharides was 107.44 mg/g, which was agreed closely with the predicted value 108.71 mg/g.

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