scholarly journals Determining the nutrient content, energy, and in vitro true digestibility of some grass forage plants

Author(s):  
Esra GÜRSOY ◽  
Adem KAYA ◽  
Mehmet GÜL

The purpose of this study was to compare the chemical composition, metabolizable energy, net energy lactation, total digestible nutrient, in vitro digestion parameters, and relative feed quality of some grass forage plants growing naturally in the meadows and pastures of Erzurum province in Turkey. Orchardgrass (Dactylis glomerata), Variegated brome (Bromus variegatus), and Intermediate wheatgrass (Agropyron intermedium) were used as the research material. In this study, the metabolizable energy (ME) and net energy lactation (NEL) contents of the green grass crops forage plants were determined by in vitro gas production method, and their digestibility parameters and relative feed quality (RFQ) by the neutral detergent fiber (NDF) procedure using an Ankom Daisy incubator. As a result of the study, the differences between the forage plants were found to be statistically significant in terms of chemical composition, metabolizable energy, net energy lactation, true organic matter digestibility (TOMD) and RFQ (P<0.05). While the green intermediate wheatgrass was found to have the highest crude protein (CP) (19.56%), crude fat (CF) (3.06%), dry matter (DM) (9.14%), ME  (8.82%), NEL (5.42%), and TOMD (97.75%); the green variegated brome was found to have the highest RFQ (149.79). The green orchardgrass was found to have the highest contents of neutral detergent fiber (NDF), acid detergent fiber (ADF), and acid detergent lignin (ADL) with 66.23%, 34.14, and 8.52%, respectively. In conclusion, the green grass forage plants examined in this study can be used to eliminate the quality roughage deficit.

2021 ◽  
Vol 42 (2) ◽  
pp. 907-920
Author(s):  
Tugay Ayasan ◽  
◽  
Ismail Ulger ◽  
Ayse Nuran Cil ◽  
Vincenzo Tufarelli ◽  
...  

This study was carried out to determine the nutritional value of some selected peanut varieties and line in the Eastern Mediterranean Agricultural Research Institute of Adana, Turkey. The peanut varieties used were Gazipasa, Sultan, NC7, Cihangir, and Halisbey; while the peanut line was DA335/2011. The chemical composition, metabolizable energy (ME), net energy lactation (NEL) and organic matter digestibility (OMD) of the selected peanut varieties and line were determined through Hohenheim in vitro gas production technique. Incubation times for Hohenheim gas production technique were 3, 6, 9, 12, 24, 48, 72 and 96 hours. The analysis of variance (General Linear Model) was carried out using the SPSS package program. The differences among groups in terms of nutrient contents were found to be significant (P<ou=0.05), except for dry matter (DM) and hemicellulose (HC). The highest crude protein (CP) (40.13%) was found in Sultan variety, while the crude oil (CO) content was found to be between 21.32 and 31.01%. The ADF, NDF, and ADL content of the peanut varieties and line were within the ranges of 2.32-7.91%, 4.85-9.88%, and 0.43-2.62%, respectively. Conversely, the Sultan variety had the highest crude cellulose (CC) value, Cihangir variety was determined to contain the highest hemicellulose (HC) value. The differences in 24 hour gas and methane production among different peanut varieties and line were found to be not-significant. (P > 0.05).


Author(s):  
Mustafa Boga ◽  
Sema Yaman ◽  
Sibel Canoğlu Doğan ◽  
Aykut Burğut

In this study nutrient content and In vitro digestibility of Mistletoe were determined in order to find out its potential to be used in the diets of ruminants. Mistletoe was collected by pruning shears during fruiting period from apricot (Purunus genus) and almond (Amygdalus communis L.) trees around the Bor road in Niğde. Four samples were taken for each tree species and later samples were divided in to four sub samples as whole plant, stalks, leaves and fruits to evaluate feed value. For the assessment of the feed value by In vitro gas production technique, rumen liquor and content was taken from a slaughter house nearby the laboratory. Mistletoe was incubated in 3, 6, 9, 12, 24, 48, 72 and 96 hours. Crude protein, crude fat, ADF and NDF content of different parts of Mistletoe plant (whole plant, stalks, leaves and fruits) collected from apricot tree were in the ranges of 90.25 and 120.82, 80.05 and 100.13, 190.29 and 330.17, 280.85 and 500.86 g kg- 1 DM, respectively while those of Mistletoe plant collected from almond trees were 100.37and 140.67, 80.42 and 100.35, 200.45 and 360.87, 340.29 and 530.10 g kg- 1 DM respectively. In vitro organic matter digestibility (DOM), metabolizable energy (ME) and net energy lactation (NEL) values of Mistletoe collected from apricot trees were ranged between 0.60 and 0.74, 9.0 and 11.17 ME kg- 1 DM and 6.08 and 7.71 NEL kg-1 DM, respectively while those of Mistletoe collected from almond trees were ranged between 0.63 and 0.76, 9.40 and 11.26 ME kg- 1 DM and 6.39 and 7.98 NEL kg- 1 DM, respectively. There were statistically differences in the DOM, ME and NEL values of different parts of the Mistletoe. In general, the mistletoe was moderate in protein and in fibre, and high in crude fat content compared with forages and very good in ME and NEL content. In conclusion, unless its harmful effects on health and performance are shown in feeding trials, mistletoe has good potential as a feed ingredient in the diets of ruminants.


2017 ◽  
Vol 26 (1) ◽  
pp. 47 ◽  
Author(s):  
Mahmoud F. Seleiman ◽  
Shaimaa Selim ◽  
Seija Jaakkola ◽  
Pirjo S.A. Mäkelä

Maize cultivation for silage could be a sustainable option in Boreal conditions, especially when combined with nutrient recycling. Effects of digestate (sludge from biogas of domestic origin) application in comparison with synthetic fertilizer and two maturity stages on chemical composition and in vitro digestibility of whole-crop maize were investigated. Starch, neutral detergent fiber, water soluble carbohydrate (WSC) and digestible organic matter (DOM) contents of maize did not differ in response to the two fertilizer treatments. However, starch, DOM and metabolizable energy of maize increased, while ash, crude protein and WSC contents decreased with increasing maize maturity. Heavy metals in maize fertilized with digestate remained low. The results indicate that whole-crop maize fertilized with digestate and harvested at 150 days after sowing is a promising feed and has good nutritive value, even in Boreal conditions.


2017 ◽  
Vol 62 (No. 10) ◽  
pp. 417-425
Author(s):  
L. He ◽  
H. Wu ◽  
W. Chen ◽  
Q. Meng ◽  
Z. Zhou

The effects of sulfur on the fermentation characteristics of corn distiller’s dried grains with solubles (DDGS) in in vitro culture were investigated. Samples (DDGS) were analyzed for nutrient values and then two independent in vitro experiments were conducted to study the effects of various sulfur sources (Na<sub>2</sub>S, Na<sub>2</sub>S<sub>2</sub>O<sub>4</sub>, Na<sub>2</sub>SO<sub>3</sub>, and Na<sub>2</sub>SO<sub>4</sub>) and different sulfur levels (0.346, 0.692, and 1.038%) on the fermentation characteristics of DDGS. Based on sampling and chemical composition analysis, there existed a great variation in the concentrations of sulfur and proximate nutrients of DDGS. In Experiment 1, sulfur source showed a significant (P &lt; 0.01) effect on the gas production parameters (asymptotic gas production (b) and gas production rate (c)) and gas production of DDGS – sulfur from Na<sub>2</sub>SO<sub>4</sub> and Na<sub>2</sub>S produced more (P &lt; 0.01) gas within 48 h with a faster gas production rate as well as higher digestibilities (dry matter degradability and organic matter digestibility) and more energy supplies (metabolizable energy), net energy for maintenance and gain, and net energy for gain than sulfur from Na<sub>2</sub>SO<sub>3</sub> and Na<sub>2</sub>S<sub>2</sub>O<sub>4</sub>. Neither ammonia-nitrogen (NH<sub>3</sub>-N) concentration nor volatile fatty acids (VFA) profile (total VFA and individual VFA proportion) were affected by sulfur source (P &gt; 0.05). In Experiment 2, no significant (P &gt; 0.05) effect on the fermentation characteristics of DDGS with increasing sulfur content was found. The collective findings suggest that regular chemical analyses are necessary to make full use of DDGS, and that the valence state of sulfur in DDGS exerts an effect on its in vitro fermentation characteristics and there appears no dose-related effect of sulfur on the fermentation of DDGS in a short-term in vitro culture.


2010 ◽  
Vol 148 (6) ◽  
pp. 723-733 ◽  
Author(s):  
H. KHALILVANDI-BEHROOZYAR ◽  
M. DEHGHAN-BANADAKY ◽  
K. REZAYAZDI

SUMMARYThe current study was conducted to determine chemical composition, nutrient content and availability, metabolizable energy (ME) content and nutritive value of sainfoin hay for ruminants. Three ruminally cannulated Holstein cows were used forin situandin vivoexperiments, to determine rumen degradability and digestibility of sainfoin hay. Apparent total tract digestibility of nutrients was determined with feeding of sainfoin hay as the sole diet to achieve 10% more than maintenance energy requirements. Six Zandi ewes were used in the palatability experiment. Means for dry matter (DM), organic matter (OM), crude protein (CP), neutral detergent fibre (NDF), acid detergent fibre (ADF) and condensed tannins (CTs) of sainfoin hay were: 940·4 g/kg and 93·43, 12·13, 47·87, 43·33 and 2·13 g/kg DM, respectively.In situeffective degradability of CP and DM were 0·38 and 0·54 g/g with a ruminal outflow rate of 0·05/h, respectively. OM apparent digestibility was in the range of 0·592–0·689, respectively, for Tilley & Terry and total faecal collection assays. ME content of sainfoin hay, according to different methods (gas production,in vitroandin vivodetermined digestible organic matter in dry matter (DOMD)) was in the range 6·87–10·11 MJ/kg DM. Metabolizable protein (MP) content was 483·4 g/kg CP. Sainfoin was more palatable than alfalfa for sheep. It was concluded that sainfoin has a potential use in ruminant rations, especially if environmental conditions are not suitable for alfalfa production.


2018 ◽  
Vol 3 (3) ◽  
pp. 953-961 ◽  
Author(s):  
William P Weiss ◽  
Alexander W Tebbe

Abstract The California Net Energy System (CNES) used a combination of measured and tabular metabolizable energy (ME) values and changes in body composition gain to determine net energy requirements for maintenance and gain and their corresponding dietary concentrations. The accuracy of the CNES depends on the accuracy of the feed ME values. Feed or diet ME values can be measured directly but are expensive and require specialized facilities; therefore, most ME values are estimated from digestible energy (DE) values, which are often estimated from the concentration of total digestible nutrients (TDN). Both DE and TDN values are often from tables and not based on actual nutrient analysis. The use of tabular values eliminates important within-feed variation in composition and digestibility. Furthermore, the use of TDN to estimate DE does not account for important variation in the gross energy value of feeds. A better approach would be to estimate DE concentration directly from nutrient composition or in vitro (or in situ) digestibility measurements. This approach incorporates within-feed variation into the energy system and eliminates the issues of using TDN. A widely used summative equation based on the commonly measured feed fractions (ash, crude protein, neutral detergent fiber, and fat) has been shown to accurately estimate DE concentrations of many diets for cattle; however, deficiencies in that equation have been identified and include an overestimation of DE provided by fat and an exaggerated negative effect of intake on digestibility. Replacing the nonfiber carbohydrate term (which included everything that was not measured) in the equation with measured starch concentration and residual organic matter (i.e., nonfiber carbohydrate minus starch) should improve accuracy by accounting for more variation in starch digestibility. More accurate estimates of DE will improve the accuracy of ME values, which will ultimately lead to more accurate NE values.


2017 ◽  
Vol 39 (3) ◽  
pp. 289 ◽  
Author(s):  
Paula Martins Olivo ◽  
Geraldo Tadeu dos Santos ◽  
Luís Carlos Vinhas Ítavo ◽  
Ranulfo Combuca da Silva Junior ◽  
Eduardo Souza Leal ◽  
...  

Agroindustrial co-products are a viable alternative for use in animal nutrition. Tests were conducted using eight different types of co-products and feed to evaluate the chemical composition, in vitro digestibility of dry matter, crude protein and neutral detergent fiber, and gas production by them. The co-products tested were: coffee hulls; pelleted citrus pulp; grape residue; soybean hulls; cottonseed; cassava foliage; and foods usually supplied to ruminants: corn silage and ground corn concentrate. Data of in vitro digestibility of dry matter, crude protein and neutral detergent fiber were tested by analysis of variance using the least square method; the results of gas production were interpreted by a non-linear regression by the Gauss-Newton method; and the effects of treatments were evaluated by the Tukey’s test. The coefficients of in vitro digestibility of dry matter, crude protein and neutral detergent fiber of co-products were different. Gas production was also different between co-products and feeds evaluated for the volume of gas produced from the fast and slow degradation fractions, degradation rate, bacterial colonization time, and the total volume of gas produced. The evaluated co-products exhibited greater in vitro dry matter digestibility compared to corn silage, except for cottonseed, grape residue, and cassava foliage. Co-products showed higher values of in vitro crude protein digestibility compared to corn silage, and a reduced in vitro digestibility of neutral detergent fiber, except for pelleted citrus pulp and soybean hulls. Corn silage produced larger volume of gas from the fast degradation fraction compared to the co-products and corn concentrate. Co-products analyzed had appropriate nutritional characteristics according to the techniques applied and can be included in ruminant diets. 


2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 76-76
Author(s):  
Yulin Ma ◽  
Xu Chen ◽  
Jianxin Xiao ◽  
Shuai Liu ◽  
Jingjun Wang ◽  
...  

Abstract The objective of this trial was to evaluate the effects of urea and calcium hydroxide on the chemical composition and in vitro ruminal fermentation of rice stover. Rice stover was stored in polyethylene bags (35 × 25 cm, 350g per bag) with: (i) no additive (CON); (ii) 4% calcium hydroxide (CA, dry matter (DM) basis); (iii) 2.5% urea and 4% calcium hydroxide (UCA, DM basis); and (iv) 9% corn steep liquor and 2.5% urea and 4% calcium hydroxide (CUCA, DM basis). Dry matter were kept at the same level at 55% for all treatments. Totally, 36 bags (4 treatments × 3 store periods × 3 repeats) were prepared and stored at ambient temperature (25 ± 3 °C). The chemical composition was measured at day 7, 10 and 15 after storage. And the in vitro ruminal fermentation was tested in vitro at d 15. Date was subjected to two-way analysis of variance (ANOVA) with the fixed effects of treatments, times and treatments × times by using SPSS 24.0. All alkali treatments reduced neutral detergent fiber (NDF) and acid detergent fiber (ADF) content (P &lt; 0.05), and increased the in vitro DM digestibility (IVDMD) of the rice stover compared with the CON. The in vitro NDF degradability (IVNDFD) and ADF degradability (IVADFD) were increased in UCA and CUCA rice stover (P &lt; 0.05) compared to others. The highest acetic acid (AA), propionic acid (PA), butyric acid (BA), total volatile acids (TVFA) concentrations and 48-h cumulative gas production were observed in CUCA rice stover (P &lt; 0.05). With the delay of storage time, dry matter (DM) and crude protein (CP) were decreased in CA and CUCA groups (P &lt; 0.05). Results in this study suggested that 9% corn steep liquor and 2.5% urea and 4% calcium hydroxide might be an effective treatment combination.


2020 ◽  
Vol 40 (1) ◽  
pp. 179-190
Author(s):  
K. O. Yusuf ◽  
O. A. Isah ◽  
O. M. Arigbede ◽  
A. O. Oni ◽  
C. F. I. Onwuka

Studies were conducted to evaluate the nutritive value of eight selected forages (Tridax procumbens, Merremia aegyptia, Aspilia africana, Tithonia diversifolia, Alchornea cordifolia, Alchornea laxiflora, Synedrella nodiflora, and Newbouldia laevis) consumed by ruminants in South-Western Nigeria. Chemical composition and qualitative analysis of saponins, phenol and steroids of the plants were determined. In vitro gas production (IVGP) was carried out for 72 hours on the plants. Metabolizable energy (ME), Organic matter digestibility (OMD) and Short chain fatty acids (SCFA) were predicted and methane (CH4) was measured. Five of the plants were subjected to acceptability study using cafeteria method. The result of the chemical composition revealed that M. aegyptia had the highest value of CP (22.09 %) while Tridax procumbens had the lowest (10.50 %). A. africana had the highest content of Calcium, Phosphorus and Zinc with values of 3.10 %, 0.93 % and 39 ppm respectively. The analysis of secondary metabolites showed that A. africana, T. diversifolia and S. nodiflora were high in saponin while A. cordifolia, A. laxiflora, T. diversifolia and A. africana were implicated for condensed Tannin. N. laevis recorded a high level of steroids. Results showed significant variations in the values of IVGP, ME, OMD, SCFA and CH4 obtained for the plants. The order of preference of the plants by the calves were M. aegyptia>A. cordifolia > N. laevis > A. axiflora> T. diversifolia. The study revealed that the forages are rich in crude protein as well as micro and macro minerals. They are also rich in highly fermentable carbohydrates which affirm them as ruminant feed resource.


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