What do parents think of using informational videos to support recruitment for parenting trials? A qualitative study

Background Lower than expected recruitment and retention rates are common challenges in parenting trials—particularly for community-based trials targeting parents of young children that rely on face-to-face recruitment by frontline workers. Recruitment requires parental informed consent, yet information sheets have been criticized for being lengthy and complex, and particularly challenging for parents with low literacy. Recent innovations include ‘talking head’ information videos. This paper aims to explore parent perceptions of using a ‘talking head’ video to support informed consent, recruitment, and retention procedures in parenting trials. Methods We conducted semi-structured interviews with a sample of 24 mothers recruited after their final follow-ups in two different parenting trials in Denmark. Before consenting to participate in the trials, parents were invited to view a video of a member of the study team giving information about the study, and again before the interviews for the current study. The audio data was transcribed and thematic analysis was conducted. Results We identified three overarching themes: (1) general impression of the video, (2) thoughts on participation in research, and (3) recruitment and retention. Participants were generally positive in their appraisal of the two talking head informational videos. We found that participants felt that a mix of paper-based and video-based sources of information would enable them to make an informed choice about whether to participate in a research study. We also found that a professionally produced video featuring a key member of the study team produced a feeling of commitment to the study that could impact retention rates. Conclusions Informational videos are acceptable to parents; however, co-production or participant/patient involvement in the development of such videos is recommended. Informational videos may not increase recruitment but have the potential for improving retention. Key design recommendations are to ensure a ‘professional’ look to the video, to supplement videos with paper-based information, to keep the length to < 3 min, and for the ‘talking head’ part to feature a key member of the study team.

Abiotic Factors Effect on the Distribution and Density of Species of the Families Unionidae, Pisididae, Euglesidae and Corbiculidae in High Mountains of the Kashkadarya Riverside Zone

As a result of the study, it has been identified that the water temperature in the Kashkadarya River is 18–24 °C, the water clarity is 0.51–0.62 m and the water flow rate is 0.72–0.84 m/sec. The species of bivalve mollusks Sinanodonta gibba, S. orbicularis, S. puerorum, Colletopterum cyreum sogdianum, Corbicula cor, C. fluminalis, C. purpurea, Corbiculina tibetensis, C. ferghanensis are widespread in the region. The abiotic factors discussed in the paper play an important role in the distribution of mollusks. There are 13 species of bivalve mollusks in the mountainous region, which is the head part of the river, 9 species in the middle part of the hill region of the river and 6 species in the lower desert region.

Evaluate the Pattern of Firearm Injuries Based on Gender in Hyderabad, Sindh, Pakistan

Aim: To assess the pattern of firearm injuries and different effecting factors based on gender in Hyderabad, Sindh, Pakistan. Study Design: Retrospective and descriptive Place and duration of study: Depart. Forensic Medicine & Toxicology, BMC LUMHS Jamshoro 01-01-2017 to 31-12-2019. Methodology: Three hundred and nineteen autopsies based on purposive sampling method were revealed the death due to firearm injuries. The demographic information, pattern of death due to firearm and which part of body involved based on variables. Results: There were 267 males and 52 were females and 95 firearm autopsies were between 31 to 40 years. It was revealed that in 38 autopsies, head was involved, 78 autopsies chest was involved, 76 autopsies abdomen was involved while 267 were from homicidal. Moreover, the cause of death due to firearm in maximum patients was hemorrhage followed by septicemia in 106 autopsies. According to gender wise, 38 autopsies involved head part 30 were male and 8 were female, 78 autopsies involved chest part 65 were male and 13 were female, 76 autopsies involved abdomen part 66 were male and 10 were female. Conclusion: The high impact on the health care setups of country, which needs the immediate attention. The firearm injuries have an impact on young and older adult population. Key words: Firearm injury, Gender, Age

Rotating Object Detection in Remote-Sensing Environment

Deep learning models have become the mainstream algorithm for processing computer vision tasks. In object detection tasks, the detection box is usually set as a rectangular box aligned with the coordinate axis, so as to achieve the complete package of the object. However, when facing some objects with large aspect ratio and angle, the bounding box has to become large, which makes the bounding box contain a large amount of useless background information. In this study, a different approach is taken, using a method based on YOLOv5, the angle information dimension is increased in head part and angle regression added at the same time of the border regression, combining ciou and smoothl1 to calculate the bounding box loss, so that the resulting border box fits the actual object more closely. At the same time, the original dataset tags are also preprocessed to calculate the angle information of interest. The purpose of these improvements is to realize object detection with angles in remote-sensing images, especially for objects with large aspect ratios, such as ships, airplanes, and automobiles. Compared with the traditional object detection model based on deep learning, experimental results show that the proposed method has a unique effect in detecting rotating objects.

Substitution Arg140Gly in Hemagglutinin Reduced the Virulence of Highly Pathogenic Avian Influenza Virus H7N1

The H7 subtype of avian influenza viruses (AIV) stands out among other AIV. The H7 viruses circulate in ducks, poultry and equines and have repeatedly caused outbreaks of disease in humans. The laboratory strain A/chicken/Rostock/R0p/1934 (H7N1) (R0p), which was previously derived from the highly pathogenic strain A/FPV/Rostock/1934 (H7N1), was studied in this work to ascertain its biological property, genome stability and virulent changing mechanism. Several virus variants were obtained by serial passages in the chicken lungs. After 10 passages of this virus through the chicken lungs we obtained a much more pathogenic variant than the starting R0p. The study of intermediate passages showed a sharp increase in pathogenicity between the fifth and sixth passage. By cloning these variants, a pair of strains (R5p and R6p) was obtained, and the complete genomes of these strains were sequenced. Single amino acid substitution was revealed, namely reversion Gly140Arg in HA1. This amino acid is located at the head part of the hemagglutinin, adjacent to the receptor-binding site. In addition to the increased pathogenicity in chicken and mice, R6p differs from R5p in the shape of foci in cell culture and an increased affinity for a negatively charged receptor analogue, while maintaining a pattern of receptor-binding specificity and the pH of conformational change of HA.

Morphological Differences In Tardigrade Spermatozoa Induce Variation In Gamete Motility

BackgroundFertilization is an event at the beginning of ontogeny. Successful fertilization depends on strategies for uniting female and male gametes that developed throughout evolutionary history. In tardigrades, investigations of reproduction have revealed that released spermatozoa swim in the water to reach a female, after which the gametes are stored in her body. The morphology of the spermatozoa includes a coiled nucleus and a species-specific-length acrosome. Although the mating behaviour and morphology of tardigrades have been reported, the motility of male gametes remains unknown. Here, using a high-speed camera, we recorded the spermatozoon motilities of two tardigrades, Paramacrobiotus sp. and Macrobiotus shonaicus, which have longer and shorter spermatozoa, respectively.ResultsThe movement of spermatozoa was faster in Paramacrobiotus sp. than in M. shonaicus, but the beat frequencies of the tails were equal, suggesting that the long tail improved acceleration. In both species, the head part consisting of a coiled nucleus and an acrosome did not swing, in contrast to the tail. The head part of Paramacrobiotus sp. spermatozoa swung harder during turning; in contrast, the tail of M. shonaicus moved more widely than the head. Finally, after mating, the spermatozoa that reached the female aggregated around the cloaca while waiting to enter her body in both tested species.ConclusionsThis study provides results for the first observations and analyses of individual spermatozoon motility in tardigrades. A comparison of the spermatozoon movements of the two tardigrades suggested that the motilities of the male gametes were affected by morphological differences, where the longer spermatozoa swam faster and the shorter ones showed more stable swimming. Swimming was mainly induced by tail movement, but the long head of Paramacrobiotus sp. spermatozoa might be especially important for turning. In addition, observations of mated female cloacae suggested that the head parts of the spermatozoa were required for aggregation around the cloaca of a mated female.

Substitution Arg140Gly in HA/H7 Reduced the Virulence Highly Pathogenic Avian Influenza Virus H7N1

The H7 subtype of avian influenza viruses (AIV) stands out among other AIV. The H7 viruses cir-culate in ducks, poultry, equine and have repeatedly caused outbreaks of disease in humans. In or-der to study the pathogenicity factors of H7N1 viruses, several variants were obtained, starting with laboratory strain, with a history of 12 passages through chicken embryos. This strain, A/chicken/Rostock/R0p/1934(H7N1) (R0p) had only 3 substitution in HA relatively A/Chicken/Rostock/45/34(H7N1), substitution Arg140Gly among them. 10 variants of this strain was obtained and studied to ascertain its biological property, genome stability and factors of patho-genicity. Strain R0p had decreased virulence for chicken, comparing with described in literature virulence of A/FPV Rostock/34 and A/chicken/Rostock/34 viruses. After 10 passages through the chicken lungs variant was obtained much more pathogenic than the starting R0p. The study of in-termediate passages through the chicken lungs showed that the jump in pathogenicity had occurred sharply between the fifth and sixth passage. By cloning these variants, a pair of strains (R5p and R6p) were obtained, and the complete genomes of these strains were sequenced. Single amino acid substitution was revealed, namely reversion Gly140Arg in HA1. This amino acid is located at the head part of the hemagglutinin, adjacent to the receptor-binding site. In addition to the increased pathogenicity for chicken and mice, R6p differs from R5p in the pattern of foci in cell culture and an increased affinity for a negatively charged receptor analogue, while maintaining a pattern of recep-tor binding specificity and the pH optimum of the HA conformational change.

FEATURES OF THE DAMAGING EFFECT OF HUNTING CARTRIDGES FIRED FROM THE KO-98M1 CARBINE

The aim of the experimental study was to establish the features of the wound channel in the biological simulator caused by semi-shell bullets of cartridges fired from the KO-98M1 carbine under the 8x57 JS cartridge. Results. In the first group of observations, the entrance gunshot injury had an oval or rounded shape, scalloped and precipitated by 0.80.1 mm edges with a Central tissue defect with a diameter of 6.50.5 mm. the Wound channel from the entrance injury expanded conically and reached a maximum at a distance of 6 cm. In the second group of observations, the entrance gunshot injury had a rounded shape, scalloped and precipitated by 10.2 mm edges with multiple radial tears, with a Central tissue defect with a diameter of 7.50.5 mm. The wound channel from the entrance injury expanded spherically immediately behind the skin, reaching a maximum at a distance of 4 cm. In the control group of observations, the entrance gunshot injury had a rectangular shape, scalloped and precipitated by 0.50.1 mm edges with one or two radial breaks and a Central tissue defect with a diameter of 5.50.5 mm. The wound channel throughout corresponded to the diameter of the firearm shell. Conclusion. In the course of experimental studies, it was found that the rounding of the head part of the shell shell increases the size of the wound canal cavity by about 4 times. The runway occurs at a distance of 6 cm from the input damage. A semi-shell bullet with a head shape in the form of a truncated cone leads to the formation of a runway immediately behind the layer of skin, while the size of the visible cavity of the wound channel exceeds the size of the projectile by 15 times.

Substitution Arg140Gly in HA/H7 attenuated highly pathogenic avian influenza virus FPV/Rostock/34 (H7N1)

The H7 subtype of avian influenza viruses (AIV) stands out among other AIV. H7 viruses circulate in ducks, poultry, equine and have repeatedly caused outbreaks of disease in humans. In order to study the pathogenicity factors of H7N1 viruses, several laboratory variants of the A/FPV/Rostock/34 (H7N1) strain were obtained by passages in the chicken lungs. After 10 such passages, a variant was obtained that differed from the parent virus by amino acid substitutions Val109Phe in PB2, Gln621Lys in PB1, Thr32Ala and Leu586Phe in PA Gly140Arg in HA1 and Ala101Thr in HA2 (numbering by H3), Ser82Arg in M2, Arg118Lys and Met124Arg in NS1. No differences were found in proteins NA, NP, M1 and NS2. The resulting variant was hundreds of times more pathogenic for chickens than the original laboratory variant of the virus. The study of intermediate passages showed that the jump in pathogenicity occurs sharply between the fifth and sixth passage through the chicken lungs. By cloning these variants, a pair of strains (R5p and R6p) were obtained, and the complete genomes of these strains were sequenced. Single amino acid substitution was revealed, namely Gly140Arg in HA1. It is important to emphasize that this substitution is a reversion, since Arg is located in position 140 HA1 of original the A/FPV/Rostock/34 (H7N1) virus (GenBank). This amino acid is located at the head part of the hemagglutinin, adjacent to the receptor-binding site. In addition to the increased pathogenicity, R6p differs from R5p by an increased affinity for a negatively charged receptor analogue, an increased affinity for MDCK cells, while maintaining a receptor specificity profile.

A STUDY OF MORPHOMETRIC ANALYSIS AND MEASUREMENT OF ANGLE OF TORSION OF THE HEAD OF HUMERUS AND SHOULDER ARTHROPLASTY

Back ground: Humerus is the longest of the long bones of superior extremity.It”s head articulates with scapular glenoid cavity to form the gleno-humeral/Shoulder joint.The rounded head of Humerus is too weakly supported in the scapular glenoid cavity lined by glenoid labrum which makes it the most mobile and most unstable nd joint and so most commonly dislocated joint of the human body.Also the humeral head part is the 2 most important part of shoulder arthroplasty. Aim of the study is to make a clinical evaluation of parameters used in prosthesis design for the humeral component applied in arthroplasty of the proximal upper extremity. Methods: A total of 100 (Rt -58,Lt –42 ) dry human Humerus available in the Department of Anatomy of Calcutta National Medical College,Kolkata,West Bengal and Nilratan Sircar Medical College,Kolkata,West Bengal were taken for the study.The Humeral heads are measured in supero-inferior(SI) diameter, and antero-posterior diameter(AP), The width of Humeral head (Wdth),and angle of Humeral Torsion were measured. At the very beginning the both sided Humerus taken for the study are properly numbered using permanent marker pen.The width of the Humeral head(Wdth) is measured 1stly by pressing the head in modelling dough and when the mould is prepared,semisolid plaster of paris is lled into the mould. After drying the replica of humeral head is smoothened upto the mark of anatomical neck of humerus thus proper width can be measured. The superoinferior(SI) diameter, and antero-posterior diameter(AP) were measured by digital slide callipers by placing it directly onto the surface of consequently numbered humeral heads. The width of Humeral head (Wdth ) were measured from these replica(properly numbered) humeral heads, made of plaster of paris at the middle, the highest thickness was measured. The measurements are taken via spreading slide calipers in milimeters. For measuring the angle of humeral angle of torsion ,we xedly placed the humerus on to a area over the smooth part of the vertical wall which was meeting another horizontal wall at 90 degrees angle thus the line joining the two epicondyles of humerus lies parallel to the vertical wall.Now placing the straight edge of the protractor onto the upper end of humerus the angle of humeral torsion is measured. Results: Both sided Humerae were analysed separately for Morphological measurement, and angle of Humeral torsion.Mean values for SI were 41.31+3.46mm (Right side) and 40.91+3.27mm(Left side). Mean values for AP were 38.27+3.05mm (Right side) and 37.91+2.70mm(Left side). Conclusion: This study will contribute some relevant data and help the orthopaeditians in dealing with cases of shoulder pathology and shoulder arthroplasty cases.