Effect of Rotten Butter Shock Load on Anaerobic Digestion of Chicken Manure

Anaerobic digestion is a popular method for improving fertilizing properties, but there is no report on the effect of shock load with butter on anaerobic digestion of chicken manure. Therefore, this study aimed to investigate the anaerobic digestion of chicken manure with butter addition. The volatile suspended solid (VSS) was set at 20g VSS/L with different butter additions from 0 to 60 g VSS/L and different oxygen flow rate (OFR) from 0 to 2.5 mL/h. The results showed that ammonia ranged from 0.072 g/L to 0.082 g/L, while the volatile acids ranged from 425 mg/L to 325 mg/L. The volatile organic acid was significantly influenced by a change in OFR compared to ammonia, while a correlation between hydrogen and hydrogen sulfide was observed. The results showed that the highest hydrogen and methane production was obtained at butter addition of 30 g VSS/L with OFR 1.4 mL/h with volumes of 78 mL and 25 L respectively. In addition, hydrogen sulfide emissions induced rapid growth with increase in butter concentration.

ANALYSIS OF BIOGAS COMPONENT PRODUCTION DURING ANAEROBIC DIGESTION OF SOUR CABBAGE IN MICROAERATION CONDITIONS UNDER DIFFERENT pH CONDITIONS

In the article, were checked influences of microaeration, pH, and VSS (Volatile Suspended Solid) for sour cab-bage anaerobic digestion. Results fermentation of sour cabbage under the condition of small oxygen addition are presented in this research can be classified as dark fermentation or hydrogenotrophic anaerobic digestion. The investigations were carried out for two concentrations 5 g VSS /L and 10 g VSS /L of sour cabbage at pH 6.0. The oxygen flow rates (OFR) for 5 g VSS /L were in the range of 0.53 to 3.3 mL/h for obtaining 2% to 8% of oxygen. In cases of low pH and microaeration, ethylene production was observed at a level below 0.05% in biogas. The highest volume of hydrogen for 5 g VSS/L was obtained for flow rate 0.58 O2 mL/h, giving hydrogen concentration in biogas in the range of 0 to 20%. For VSS 5 g/L and oxygen flow rate 0.58 mL/h; 0.021 L of hydrogen is produced per gram of VSS. In this case, VSS 10 g/L and oxygen flow rate 1.4 mL/h at pH 6.0, 0.03 L of hydrogen is generated per gram. Microaeration from 0.58 mL/h to 0.87 mL/h was propitious for hydrogen production at 5 g VSS/L of sour cabbage and 1.4 mL/h for 10 g/L. Another relevant factor is the volatile suspended solid factor of sour cabbage that caused optimal hydrogen production at VSS 89.32%.

Utilization of Aquatic Weed Salvinia molesta as a Raw Material for Biogas Production

Salvinia molesta is an aquatic weed having very high growth rate. The high abundance of this species biomass could be utilized as a source of alternative energy. This research was aimed to utilize S. molesta as a substrate for biogas production using batch system in order to reduce euthrofication effect in a lake. Cow manure was used as starter for anaerobic process because containing bacteria. Proximate test was conducted to analyze the chemical characteristics of the aquatic weed. Chemical oxygen demand, total solid, and total volatile suspended solid of weed and manure were analyzed according to the APHA 2012 method. Biogas composition was analyzed with gas chromatography. The results showed that the S. molesta contained high lignin content and low C:N ratio. Hydrolisis and acidification process occured very rapid, resulting in an increase of the weed and manure mixture COD. In the other hand, the decreased of COD removal was caused by the massal death of methanogenesis bacteria. The total solid and total volatile suspended solid content were not significantly decreased. Potential biogas production from S. molesta was 58.16 L.kg-1 with 318.29 mL.gram VS-1day-1 of methane production. This production value was still low or compared to that of cow manure which have been established as biogass materials and need modification to improve the biogas production.

BIODEGRADASI REMAZOL BRILIANT BLUE DALAM SISTEM BIOFILTRASI VERTIKAL DENGAN INOKULUM BAKTERI DARI SEDIMEN SUNGAI MATI IMAM BONJOL DENPASAR

BIODEGRADATION REMAZOL BRILLIANT BLUE IN VERTICAL FILTRATION SYSTEM WITH INOKULUM BACTERIA FROM DIED SEDIMEN RIVER IMAM BONJOL DENPASARThe biodegradation research of Remazol Brilliant Blue (RBB) has been done in vertical biofiltration systems with bacterial inoculum that had seed from soil Mati River Imam Bonjol Denpasar. This aims of research are to obtain the best active suspension grown from soil samples of Mati river sediment and to determine the magnitude of the effectiveness and capacity of biosystem . The artificial waste water of RBB has made with a concentration of 200 mg/L. In the first phase, the best active suspension is obtained by determining the value of VSS ( Volatile Suspended Solid ) is the highest as a source of inoculum of bacteria capable of degrading RBB. While the second phase, the effectiveness and capacity of biosystem is obtained by determining the levels of waste of artificial RBB vertical biofiltration system (biosystem). Biofilm has made with attached bacteria consortium in volcanic rock for 7 days. RBB subsequently incoporated into it to determine the concentration of it and to determine the effectiveness and capacity and identified the bacteria contained in biosystem. The results showed the best sludge active from soil sediments of the Mati River Imam Bonjol Gang Keladian with a value of Volatile Suspended Solid (VSS) highest of 17200 mg/L when the sreeding time of 48 hours. The results of processing using biosystem known to decreased when the processing time from 6 hours up to 114 hours, from concentration of 200 mg/L to 19.6211 mg/L. Then the prosses has increased again into 19.8209 mg/L at the time to 120 hours. The highest effectivity of biosystem to degrading remazol brilliant blue of 90.19 % for 114 hours , while the highest capacity is obtained from the biosystem is 1.6525 x mg /g for 114 hours. The bacteria that act to decreased identified as Pseudomonas sp., Aeromonas sp . and Plesiomonas sp, with Pseudomonas sp more dominant in the degrading of dye RBB. The value of the colonists before degradation of 7.2 x CFU/gr and the value after the degradation of 2.6 x CFU/gr.

Pengolahan Air Limbah Kadar Garam Tinggi dengan Sistem Lumpur Aktif

Air limbah perebusan dari industri kacang garing masih menjadi permasalahan karena mengandung garam yang tinggi. Pada kegiatan penelitian ini dilakukan pengolahan air limbah tersebut dengan sistem lumpur aktif dengan memanfaatkan mikroba yang sudah teradaptasi dengan kadar garam tinggi berasal dari bak equalisasi air limbah tersebut. Percobaan penelitian pengolahan limbah dilakukan dengan dua kondisi yaitu kadar MLVSS (Mixed Liquour Volatile Suspended Solid) awal 1000 mg/L dan 2000 mg/L, pengamatan sampel dilakukan setiap 24 jam selama lima hari. Hasil analisis menunjukkan bahwa terdapat penurunan nilai BOD dan permanganat pada sampel air limbah namun tidak terjadi penurunan untuk parameter nilai klorida. Penurunan nilai BOD adalah sebesar 89,89% dengan perlakuan MLVSS 1000 mg/L dan 91,01 % untuk perlakuan MLVSS 2000 mg/L. Kondisi optimal pada sistem lumpur aktif belum tercapai yang ditunjukkan dari nilai sludge volume masih kurang dari 30%. Sampel hasil pengolahan belum dapat memenuhi baku mutu yang dipersyaratkan dengan nilai BOD 352,2 mg/L, permanganat 213,4 mg/L dan klorida 8347 mg/L.

Optimization and Modelling of Denitrification and Methanogenic Activity in Start up of Mixotrophic Anammox Reactor

In the present study, the SDA (specific denitrification activity) and SMA (specific methanogenic activity) in Start up of mixotrophic anammox reactor was optimized by applying the response surface method (RSM). The purpose of this work was to find the optimal combination of C/N ratio, influent ammonium (NH4+-N) and volatile suspended solid (VSS) with respect to minmum the SDA and SMA. Based on the RSM results, the quadratic model developed for the responses indicated that optimal conditions were C/N ratio of 0.5, influent NH4+-N content of 200mg L-1, and VSS content of 59.31g L-1. Under this conditions, the SDA and SMA were minimize and found to be 0.05 mmol N2 (g VSS d)-1, 0.017 mmol N2 (g VSS d)-1, respectively

Combination of High Pressure and Thermal Hydrolysis Pretreatment Effect on Sludge

In 110-190°C for 15-75 min of excess sludge thermal hydrolysis experiment, inspected the volatile suspended solid dissolution rate, the concentration of sludge SCOD, TCOD, components of organic acids in the supernatant fluid changes,such as sewage thermal hydrolysis characteristics, analysis the effect to improve the performance of sludge anaerobic digestion. The results show that with the increase of thermal hydrolysis temperature and time, SCOD and VFA in the sludge supernatant on rising. Under the condition of 190°C,75min, SCOD and VFA reached a maximum of 6674 mg/L,2630 mg/L; Release of sludge organic dissolved solids,COD and other major completed in the first 45 min,after 45 min changed little; Thermal hydrolysis temperature was between 90~170°C,sludge anaerobic digestion performance increases with temperature,when the temperature was higher than 170°C,sludge anaerobic digestion performance began to decline.

Enhancing the hydrolysis of excess sludge using thermophilic Bacillus sp. Hnu under different oxygen supply conditions

A thermophilic Bacillus strain was isolated from excess sludge in the present study. The 16S rDNA analysis indicated that this strain was a Bacillus sp. and has not been reported previously (named Bacillus sp. Hnu). The aim of this paper was to investigate the enhanced efficiency of excess sludge hydrolysis by the addition of thermophilic Bacillus sp. Hnu under different oxygen supply conditions. The results indicated that higher temperature and more oxygen supply was advantageous to the volatile suspended solid removal ratio with the same effect to that of protease activity. The maximum volatile suspended solid removal ratio was achieved at 21.5 %, 42.5 %, and 54.4 % after 108 h digestion at pH 6.9 and 60?C and increased by 17.2 %, 38 %, and 45.4 % under anaerobic, microaerobic, and aerobic conditions compared with the control test, respectively. The hydrolysis rate constants for the anaerobic, microaerobic, and aerobic conditions were 3, 4.8, and 7 times (40?C) and 3.5, 9.8, and 11.8 times (50?C) and 2.7, 7.2, and 10.3 times (60?C). Hydrolysis performance indicated that the Bacillus sp. Hnu could accelerate the hydrolysis rate. The kinetic study showed that the hydrolysis of sludge with Bacillus sp. Hnu and the control test followed the first-order kinetics except at 60?C.

Nitrogen removal in an upflow sludge blanket (USB) reactor combined by aerobic biofiltration systems

A new nitrogen removal process (up-flow sludge blanket and aerobic filter, USB-AF) was proposed and tested with real sewage. In the USB reactor, the larger part of influent organic and nitrogen matters were removed, and ammonia was effectively oxidized in the subsequent aerobic filter. The role of the aerobic filter was to convert ammonia into nitrate, an electron acceptor that could convert soluble organic matters into volatile suspended solid (VSS) in the USB. The accumulated as well as influent VSS in the USB was finally degraded to fermented products that were another good carbon source for denitrification. Total COD, settleable COD and soluble COD in the raw sewage were 325, 80 and 140mg/l, respectively. Most unsettleable COD as well as some SCOD in the influent was successfully removed in the USB. TCOD removal in the anoxic filter was by denitrification with the recycled nitrate. Low COD input to the aerobic filter could increase nitrification efficiency, reduce the start-up period and save the aeration energy in the USB-AF system. About 95% of ammonia was nitrified in the aerobic filter with no relation to the influent ammonia concentration. Denitrification efficiency of the recycled nitrate in the anoxic filter was about 85, 83, and 72% at recycle ratios of 100, 200, and 300%, respectively. T-N removal efficiency was 70% at recycle ratio of 300%.