KLT BIOAUTOGRAFI HASIL PARTISI EKSTRAK ETANOL HERBA BANDOTAN (Ageratum conyzoides L.) TERHADAP Shigella dysentriae

Penelitian uji daya hambat dan analisis KLT bioautografi hasil partisi ekstrak etanol herba bandotan (Ageratum conyzoides L.) terhadap Shigella dysentriae telah dilakukan. Penelitian ini bertujuan untuk mengetahui kemampuan ekstrak tersebut dalam menghambat pertumbuhan S.dysentriae dan membandingkan daya hambat ekstrak dengan tingkat kepolaran yang berbeda yaitu pada ekstrak n-heksana, larut etil asetat, dan tidak larut etil asetat berdasarkan pengukurandiameter hambatan yang terbentuk. Herba tersebut diekstraksi dengan etanol menggunakan metode maserasi, lalu dipartisi dengan pelarut n-heksana dan etil asetat. Pengukuran dilakukan dengan menggunakan metode difusi pada medium Miller Hinton Agar (MHA) dengan waktu inkubasi 24 jam pada suhu 37oC dan memberikan diameter daerah hambatan terbesar pada ekstrak tidak larut etil asetat herba bandotan yaitu 9,3 dan 10,3 mm. Pemisahan secara KLT pada ekstrak n-heksana, ekstrak etil asetat, dan ekstrak tidak larut etil asetat dengan cairan pengelusi berturut-turut, n-heksana:etil asetat (3:1), n-heksana:etil asetat (1:1), dan etil asetat:etanol (10:1) dengan jumlah bercak noda berturut-turut 4, 5, dan 2. Nilai Rf pada ekstrak n-heksana 0.25, 0.41, 0.52, dan 0.71 sedangkan pada ekstrak etil asetat 0.34, 0.53, 0.65, 0.81, dan 0.92 pada ekstrak tidak larut etil asetat 0.33 dan 0.64. Hasil KLT bioautografi diperoleh komponen antibakteri yang diidentifikasi ekstrak n-heksana adalah golongan steroid dan pada tidak larut etil asetat golongan polifenol ABSTRACTInhibition test research and TLC bioautographic bioassay method of the partition results of the ethanol extract of bandotan (Ageratum conyzoides L.) herb against Shigella dysentriae have been conducted. This study aims to determine the ability of these extracts to inhibit S.dysentriae growth and to compare the inhibition of extracts with different polarity levels, n-hexane soluble, ethyl acetate soluble, and ethyl acetate insoluble extracts based on the diameter measurement of the formed resistance. The herbs were extracted with ethanol using the maceration method, then partitioned with n-hexane and ethyl acetate solvents. Measurements were carried out using the diffusion method on Miller Hinton Agar (MHA) medium with an incubation time of 24 hours at 37oC and gave the largest diameter area of resistance to the ethyl acetate insoluble extract of bandotan herb, value 9.3 and 10.3 mm. Separation by TLC on n-hexane extract, ethyl acetate extract, and ethyl acetate insoluble extract with elusive liquid respectively, n-hexane: ethyl acetate (3: 1), n-hexane: ethyl acetate (1: 1), and ethyl acetate: ethanol (10: 1) with the number of stains 4, 5, and 2, respectively. 0.81, and 0.92 in ethyl acetate insoluble extracts 0.33 and 0.64. The results of the bioautography TLC bioassay method showed that the antibacterial component identified in the n-hexane extract was a steroid compound and ethyl acetate insoluble was a polyphenol compound.

Attempt to Identify Sex Hormones in the Bodies of Selected Norway Spruce Bark Beetles

A gas chromatography technique was applied to the adults of Ips typograhus (L.) and Pityogenes chalcographus (L.) collected from pheromone traps placed in Norway spruce stands in southern Poland in distinguished population swarming periods for the qualitative and quantitative determination of steroid compound differences between insect sexes. Ten not yet identified for bark beetle compounds from the group of sterols, including cholestenone, 4,6-cholestadiene-3-one, choles-4-en-3,6-dione and 17β-Hydroxyandrosta-1,4-dien-3-one benzoate, which can potentially act as gender hormones were detected. The presence of ecdysone and 20-hydroxyecdysone in the bodies of the studied bark beetles was confirmed. However, slight differences in the content of ecdysteroids in the bodies of males and females may be only the remains of the insect’s metamorphosis. Due to the small differences in the extracted compounds between the females and males, their variability in concentrations during the swarming period seems to be useless as a basis for sex determination.

Anti-inflammatory activities of squalene compound of methanol extract of Abroma augusta L

Abroma augusta L plant traditionally was used to treat swellings, cuts, sores, and bruises. In the province of Jambi, A. augusta is used in folk medicine to treat wounds. This study aims to isolate the steroid compound from the root of A. augusta L and determine its anti-inflammatory activities. Extraction and fractionation have been done with graded maceration using solvents with different polarities, which are n-hexane, ethyl acetate, and methanol. The separation was performed by column chromatography, followed by preparative thin-layer chromatography. The characterization of the isolate was carried out using UV-Vis spectrophotometry and infrared spectrophotometry, GC-MS. The anti-inflammatory activities of methanol extract and isolate of A. augusta was performed in this study was designed to evaluate the dose-response relationship of the anti-inflammatory activity in rat models of chronic inflammation chromatography to obtain isolate 2.1.1 that characterize and showed maximum absorbance at 265. The result of IR showed the presence of functional groups, -C=C-H, -C=H, -CH, CH3, CH2, and –CO belongs to the steroid compound. The results of the GC-MS show that isolates contain squalene compounds with a value of m/z 410, Isolate and crude extract showed an anti-inflammatory activity that almost approached the positive control of sodium 4-chlorophenolate. It could be concluded that isolate and extract provide good anti-inflammatory activity, that promise for new drug candidate squalene-based A. augusta.

The immunomodulatory potential of the arylmethylaminosteroid sc1o

Developing resistance mechanisms of pathogens against established and frequently used drugs are a growing global health problem. Besides the development of novel drug candidates per se, new approaches to counteract resistance mechanisms are needed. Drug candidates that not only target the pathogens directly but also modify the host immune system might boost anti-parasitic defence and facilitate clearance of pathogens. In this study, we investigated whether the novel anti-parasitic steroid compound 1o (sc1o), effective against the parasites Plasmodium falciparum and Schistosoma mansoni, might exhibit immunomodulatory properties. Our results reveal that 50 μM sc1o amplified the inflammatory potential of M1 macrophages and shifted M2 macrophages in a pro-inflammatory direction. Since M1 macrophages used predominantly glycolysis as an energy source, it is noteworthy that sc1o increased glycolysis and decreased oxidative phosphorylation in M2 macrophages. The effect of sc1o on the differentiation and activation of dendritic cells was ambiguous, since both pro- and anti-inflammatory markers were regulated. In conclusion, sc1o has several immunomodulatory effects that could possibly assist the immune system by counteracting the anti-inflammatory immune escape strategy of the parasite P. falciparum or by increasing pro-inflammatory mechanisms against pathogens, albeit at a higher concentration than that required for the anti-parasitic effect. Key messages • The anti-parasitic steroid compound 1o (sc1o) can modulate human immune cells. • Sc1o amplified the potential of M1 macrophages. • Sc1o shifts M2 macrophages to a M1 phenotype. • Dendritic cell differentiation and activation was ambiguously modulated. • Administration of sc1o could possibly assist the anti-parasitic defence.

Isolasi Dan Identifikasi Senyawa Metabolit Sekunder Ekstrak n-Heksana Tumbuhan Cakar Ayam (Selaginella plana Hieron)

ABSTRAK Penelitian ini bertujuan untuk mengisolasi dan mengidentifikasi senyawa metabolit sekunder yang terdapat dalam ekstrak n-heksana tumbuhan cakar ayam (Selaginella plana Hieron). Penelitian dilakukan melalui beberapa tahap yaitu preparasi sampel, maserasi dengan metanol, partisi dengan n-heksana, fraksinasi, pemurnian, dan identifikasi. Isolat diperoleh dalam bentuk kristal jarum berwarna putih dengan titik leleh 128 – 1300C dan memberikan hasil positif pada pereaksi Lieberman-Buchard. Dan spektrum IR isolat menunjukkan adanya gugus -OH (3425,58 cm-1), CH3- dan -CH2- alifatik (2958,80 cm-1; 2929,87 cm-1; 2895,15 cm-1; dan 2866,22 cm-1), C=C alkena (1641,42 cm-1), CH3- dan -CH2- tekuk (1462,04 cm-1 dan 1377,17 cm-1), C-O (1058,92 cm-1), =C-H (962,48 cm-1). Berdasarkan data tersebut disimpulkan bahwa isolat yang diperoleh merupakan golongan steroid. Kata kunci : Isolasi, Selaginella plana Hieron, Metabolit Sekunder, Steroid ABSTRACT The purpose of this research was to isolate and identifed the secondary metabolite compound from n-hexane extract of Cakar Ayam (Selaginella plana Hieron) plants. This research was carried out in several steps, they were preparation of sample, maceration with methanol, partition with n-hexane, fractination, purification, and identification. The isolate obtaned was white crystal needle shaped with melting point to 128-1300C and gave positive respon to the Liebermann-Buchard test. The IR spectrum of the isolate showed the presence of -OH (3425,58 cm-1),CH3- and -CH2- alifatic (2958,80 cm-1; 2929,87 cm-1; 2895,15 cm-1; and 2866,22 cm-1),C=C alkena (1641,42 cm-1),CH3- and -CH2- bending (1462,04 cm-1 and 1377,17 cm-1), C-O (1058,92 cm-1),=C-H (962,48 cm-1). Based on those result, it was concluded that the isolate was a steroid compound. Keywords : Isolation, Selaginella plana Hieron, Secondary Metabolites, Steroid