scholarly journals Potential of Ulva linza L. and Caulerpa racemosa var. uvifera seaweeds from Cox’s Bazar, Bangladesh as sea vegetable

2021 ◽  
Vol 56 (3) ◽  
pp. 223-230
Author(s):  
A Aziz ◽  
SK Roy ◽  
A Hassan ◽  
MZ Haque ◽  
UF Shahjadee ◽  
...  
Keyword(s):  
Nutritional Quality ◽  
River Estuary ◽  
Amino Acid Content ◽  
Essential Amino Acids ◽  
Quality Parameters ◽  
Total Amino Acid ◽  
Total Protein Content ◽  
Caulerpa Racemosa ◽  
Carotene Content ◽  
Ulva Linza

The nutritional quality parameters of leafy Ulva linza L. collected from the west coast of the Naf river estuary, Teknaf and grape-like Caulerpa racemosa var. uvifera collected from St. Martin's Island, Cox's Bazar, Bangladesh, were studied. Total protein content in U. linza (22.05±1.73%) was found significantly (p<0.05) higher than C. racemosa var. uvifera (18.20±1.50%) but with low crude lipids in the former; total amino acid content was also higher in the former, both having nearly the same amount of essential and non-essential amino acids; vitamin C content (about 2.5 mg/100 g) was nearly identical but β -carotene content was higher in the later (9.04 mg/100 g dry wt.). Crude fiber in U. linza was low (4.21±0.43%) but high in C. racemosa var. uvifera (18.20±1.50%). Other micronutrients were also ascertained. The nutritional quality parameters determined suggested that the “sea-salad” and “sea- grapes” can be used as sea-vegetables and food supplements. Bangladesh J. Sci. Ind. Res.56(3), 223-230, 2021

scholarly journals Comparative Study on Quality Parameters of Royal Jelly, Apilarnil and Queen Bee Larvae Triturate

2017 ◽  
Vol 74 (1) ◽  
pp. 51
Author(s):  
Rodica MARGAOAN ◽  
Liviu Alexandru MARGHITAS ◽  
Daniel Severus DEZMIREAN ◽  
Otilia BOBIS ◽  
Victorita BONTA ◽  
...  
Keyword(s):  
Amino Acids ◽  
Royal Jelly ◽  
Essential Amino Acids ◽  
Quality Parameters ◽  
Total Protein Content ◽  
Beneficial Effects ◽  
Long Time ◽  
Soxhlet Method ◽  
Kjeldahl Method

Given their beneficial effects in terms of health, the natural products, especially beehive products, have drawn the attention of consumers since long time ago. In order to guarantee the quality of these products on the market, their chemical composition needs to be analyzed. Thus, this current research had as objective the establishment of quality parameters for beehive brood food derived products: apilarnil and queen bee larvae triturate. These two products were compared with royal jelly which is the basis of brood food in the first 3 days of larval stage. The carbohydrates were determined by HPLC-IR and allowed the identification of seven carbohydrate compounds, predominantly glucose, fructose and sucrose. The lipid profile was analyzed by the Soxhlet method. The total protein content was determined by the Kjeldahl method. Free amino acids were analyzed by LC-MS. A total of 31 amino acids were identified of which nine are essential amino acids for humans. 

Reduction of nitrogen excretion by pigs between 20 and 50 kg live weight given low protein diets

1996 ◽  
Vol 1996 ◽  
pp. 149-149
Author(s):  
P.H. Simmins ◽  
J.P. Braund ◽  
A. Laurie ◽  
J. Pinasseau ◽  
J. Weigel
Keyword(s):  
Raw Materials ◽  
Amino Acid Content ◽  
Nitrogen Retention ◽  
Live Weight ◽  
Essential Amino Acids ◽  
Growing Pigs ◽  
Nitrogen Excretion ◽  
Total Amino Acid ◽  
Net Energy ◽  
Protein Levels

The potential reduction in nitrogenous excretion by growing pigs, through the reduction of protein levels in diets, has not been consistently achieved without compromising performance. This may have been due to inadequate formulation of the diets. Formulation on the basis of the total amino acid content of feed ingredients may limit the nitrogen retention, and hence the rate of protein accretion (Lee et al., 1993). Therefore, the objective of the experiment reported here was to evaluate the nitrogen balance of pigs fed diets at two levels of protein, formulated on the basis of true ileal digestibility of the essential amino acids (Rhône-Poulenc Animal Nutrition, 1993) and the net energy available from the raw materials (Noblet et al., 1994).

Comparison of Three Methods for Calculating Protein Content in Developing Apple Flower Buds

1992 ◽  
Vol 75 (4) ◽  
pp. 734-737 ◽  
Author(s):  
Shahrokh Khanizadeh ◽  
Deborah Buszard ◽  
Constantinos G Zarkadas
Keyword(s):  
Amino Acid ◽  
Protein Content ◽  
Conversion Factor ◽  
Amino Acid Content ◽  
Good Method ◽  
Accurate Method ◽  
Total Amino Acid ◽  
Total Protein Content ◽  
Flower Buds ◽  
Flower Bud

Abstract Three methods (Kjeldahl, sulfuric acid-hydrogen peroxide, and summation of amino acid content) for determining and calculating the protein content of apple flower buds were compared. Quantitation of protein content based on summation of amino acids appears to be the most accurate method. A new nitrogen:protein conversion factor (5.51) was calculated based on total amino acid analysis. This new conversion factor could replace the conventional 6.25 factor for estimating total protein content of apple flower bud by the Kjeldahl method. However, Kjeldahl is not an accurate method for estimating protein content in apple flower bud tissue, regardless of the conversion factor, and probably would not be a good method for estimation of protein in other plant species.

Effect of type of diet (casein-based or protein-free) and caecotrophy on ileal endogenous nitrogen and amino acid flow in rabbits

2004 ◽  
Vol 79 (2) ◽  
pp. 231-240 ◽  
Author(s):  
A. I. García ◽  
J. C. de Bias ◽  
R. Carabaño
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Amino Acid Content ◽  
Essential Amino Acids ◽  
Total Amino Acid ◽  
Total Amino Acid Content ◽  
Present Trial ◽  
Protein Free Diet ◽  
Ingredient Composition ◽  
Intake Ratio

AbstractTwenty-eight New Zealand White X Californian doe rabbits weighing 3791±141 g were surgically fitted with a glass T-cannula at ileum level. Animals were given ad libitum access to either a casein-based diet (C) or a protein-free diet (PF). The only difference in the ingredient composition of the diets was the substitution of 160g/kg of maize starch by casein in the C diet. Animals were randomly allotted to each experimental diet. Seven rabbits per diet were not allowed to practice caecotrophy. The C diet resulted in higher food dry matter (DM) intake (DMI) (111-5 v. 51-5 g/day), food nitrogen intake (3-12 v. 0-20 g/day), ileal flow of DM (56-0 v. 23-8 g/day) and ileal flow of nitrogen (0-72 v. 0-24 g/day) but lower soft faeces DMI (21-7 v. 41-4 g/day) than the PF diet. A linear relationship between ileal endogenous nitrogen flow and total DMI was found for animals given the C but not for the PF diet. The ingestion ofC diet led to a higher (proportionately 0-41, on average), endogenous ileal amino acid flow than PF diet for all amino acids except for glycine. The ileal flow of glycine was proportionately 0-25 higher in animals given the PF diet than in animals given the C diet. The C diet resulted in higher content of nitrogen, threonine and proline in soft faeces than the PF diet. In contrast, the ingestion of the PF diet led to a higher content of lysine and methionine in soft faeces than the C diet. Within the C diet, animals practising caecotrophy showed higher ileal flow of DM (73-4 v. 56-0 g/day) and phenylalanine (0-834 v. 0-677 g/kg DMI) than animals not practising it. Caecotrophy led to a higher endogenous proportion of some of the most important limiting essential amino acids in rabbits, such as arginine, lysine, phenylalanine and threonine. In rabbits, the use of PF diets has limited practical application in the estimation of nitrogen endogenous losses as the low food DMI and the high soft faecesDMI leads to abnormal food: soft faeces intake ratio. Moreover, due to the important effect of caecotrophy on the amino acid composition of endogenous losses, it is important to correct the total amino acid content of the ileal chyme by the undigested amino acid residue coming from the soft faeces intake. This correction could be made, without the methodological complexity implied, by fitting animals with a wooden collar and thereby avoiding caecotrophy as in the present trial.

EVALUATION OF PROTEIN QUALITY IN WHEAT BY-PRODUCTS: DIGESTIBILITY OF PROTEIN AND ABSORPTION OF AMINO ACIDS BY THE RAT

1968 ◽  
Vol 48 (2) ◽  
pp. 215-220 ◽  
Author(s):  
E. M. Olsen ◽  
S. J. Slinger ◽  
J. D. Summers
Keyword(s):  
Amino Acids ◽  
Protein Quality ◽  
Amino Acid Content ◽  
Protein Digestibility ◽  
Essential Amino Acids ◽  
Sole Source ◽  
Total Amino Acid ◽  
Total Amino Acid Content ◽  
Percentage Absorption ◽  
By Products

Two experiments were conducted with rats to evaluate the protein quality of a series of wheat by-products, which served as the sole source of protein in diets calculated to contain 10% protein. Digestibility of nitrogen and percentage absorption of amino acids were compared with weight gain, feed efficiency and net protein utilization (NPU) as criteria. Percentage absorption of different amino acids was found to vary considerably in the same protein. Of the essential amino acids, lysine, threonine, methionine and isoleucine had the lowest percentage absorption in all of the products tested. The results indicate that the amount of amino acids absorbed, as measured by the excretion method, may be a more satisfactory measure of protein quality than total amino acid content.

Reduction of nitrogen excretion by pigs between 20 and 50 kg live weight given low protein diets

1996 ◽  
Vol 1996 ◽  
pp. 149-149
Author(s):  
P.H. Simmins ◽  
J.P. Braund ◽  
A. Laurie ◽  
J. Pinasseau ◽  
J. Weigel
Keyword(s):  
Raw Materials ◽  
Amino Acid Content ◽  
Nitrogen Retention ◽  
Live Weight ◽  
Essential Amino Acids ◽  
Growing Pigs ◽  
Nitrogen Excretion ◽  
Total Amino Acid ◽  
Net Energy ◽  
Protein Levels

The potential reduction in nitrogenous excretion by growing pigs, through the reduction of protein levels in diets, has not been consistently achieved without compromising performance. This may have been due to inadequate formulation of the diets. Formulation on the basis of the total amino acid content of feed ingredients may limit the nitrogen retention, and hence the rate of protein accretion (Lee et al., 1993). Therefore, the objective of the experiment reported here was to evaluate the nitrogen balance of pigs fed diets at two levels of protein, formulated on the basis of true ileal digestibility of the essential amino acids (Rhône-Poulenc Animal Nutrition, 1993) and the net energy available from the raw materials (Noblet et al., 1994).

scholarly journals An investigation into copper-binding capacity of the white leg shrimp head (Litopenaeus vannamei) protein hydrolysate

2020 ◽  
pp. 13-18
Author(s):  
Tam Dinh Le Vo Vo
Keyword(s):  
Amino Acid ◽  
G Protein ◽  
Protein Hydrolysate ◽  
Binding Capacity ◽  
Amino Acid Content ◽  
Chelating Agent ◽  
Essential Amino Acids ◽  
Total Amino Acid ◽  
Copper Binding ◽  
Shrimp Head

The aim of this study is to obtain the copper-binding protein hydrolysate from the white leg shrimp head (WLSH) by-product using enzymatic hydrolysis. The outcome indicated that the copper-binding capacity (CBC) of the WLSH protein hydrolysate achieved the maximum value of 19.4±0.5 mg Cu2+/g protein with hydrolysis conditions including Flavourzyme preparation, pH 7.5, 50°C, the enzyme:substrate (E:S) ratio of 80 U/g protein and 5h of hydrolysis. Under the pH treatment in a range of 1-11 or heat treatment at 100°C for up to 180 min, its CBC remained over 80%. The water holding capacity (WHC) and the oil-holding capacity (OHC) of the protein hydrolysate were 4.1±0.1 ml water/g protein hydrolysate powder and 4.5±0.1 ml oil/g protein hydrolysate powder, respectively. The solution also encompassed up to 8 essential amino acids, accounting for 36.1% of the total amino acid content. The protein hydrolysate could serve not only as a copper chelating agent, preventing copper-deficient or superfluous relating diseases, but also as a texturizer and an amino acid supplement fortified in various types of food.

scholarly journals COMPREHENSIVE EVALUATION OF SOYBEAN AND SUNFLOWER PROCESSING PRODUCTS

2021 ◽  
Vol 22 (1) ◽  
pp. 191-196
Author(s):  
Н. P. Ryvak ◽  
G. I. Boyko ◽  
R. O. Ryvak
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Protein Content ◽  
Soybean Meal ◽  
Inorganic Nitrogen ◽  
Amino Acid Content ◽  
Total Content ◽  
Essential Amino Acids ◽  
Total Amino Acid ◽  
Sunflower Meal

The literature review of the article presents the characteristics of the protein value of plant feed materials, in particular, of soybean and sunflower processing products, depending on the production technology. Their amino acid composition ideally meets the needs of young animals and poultry due to the high content of essential amino acids. The section «Materials and methods» presents methods for determining: crude protein by the Kjeldahl method, soluble protein in 0.2% potassium hydroxide, Barnstein protein, falsification of protein products with inorganic nitrogen, as well as characteristics of methods for determining the content of essential and non-essential amino acids, incl. sulfur-containing, using the system of capillary electrophoresis «Drops-105M». As a result of the research it was found that of the total number of analyzed samples of soybean meal in terms of protein content, 30.8% of products did not meet the declared values of producers and were in the range of 37.4 - 40.8%, in terms of protein solubility were in the range of 75 - 78%, which meets the norms of feeding.In addition, 5.0% of the total number of samples analyzed, were falsified after Bard alcohol, falsification of inorganic nitrogen was not found. The use of low-protein raw materials for the production of sunflower meal and cake was reflected in their protein content and ranged from 26.3 to 33.7%. Fermented soybean product and toasted soybean meal are characterized by a higher total content of essential amino acids in the protein, which was 26.3% and 27.2%, respectively, compared with full-fat expanded soybean expanded, where this figure was at 20.25%, and prevailed other analyzed products by methionine, threonine, tyrosine, phenylalanine, proline, alanine etc. The analyzed sample of sunflower meal, produced by a special technology is equivalent in total amino acid content in the studied soy products. Its content of methionine was 1.5 and 2.1 times higher, compared with toasted soybean meal and full-fat soybean, respectively. Also, the content of threonine, glycine, cystine, tryptophan, aspartic and glutamic acids in the sample of sunflower meal prevailed the content of the same amino acids in soybean products. Thus, the comprehensive assessment of plant protein components makes it possible to control not only the quantitative content of protein and amino acids, but also provides a detailed assessment of feeding.

scholarly journals Optimization of hydrolyzation procedure for amino acid analysis in fish meat with HPLC-DAD by Response Surface Methodology (RSM)

2020 ◽  
Vol 37 (2) ◽  
pp. 113-123 ◽  
Author(s):  
Ekrem Cem Çankırılıgil ◽  
Nermin Berik ◽  
Esen Alp Erbay
Keyword(s):  
Response Surface Methodology ◽  
Amino Acid ◽  
Response Surface ◽  
Acid Content ◽  
High Performance ◽  
Amino Acid Content ◽  
Essential Amino Acids ◽  
Total Amino Acid ◽  
Array Detector ◽  
Fish Meat

In this research, with the aim of maximizing amino acid content by different hydrolization procedures of fish meat were carried out and analysed with high performance liquid chromatography (HPLC) equipped with diode array detector (DAD). Response surface methodology (RSM) was used to determine factors that used in the experiments. The RSM suggested 16 different hydrolization trials between the ranges of normality as 3 N - 8 N, temperature as 90°C-110°C and duration as 12-24 hours were run. In the following, hydrolysed samples were analysed with HPLC-DAD and obtained data were evaluated with Box-Behnken method in RSM. Our results show that, the most effective experiment was found as hydrolysing by 3.79 N at 110°C in 24 hours for total amino acid content as well as maximized essential amino acids with 0.981 desirability. For sensitive ones, hydrolysing in 3.42 N at 106.8°C in 12.02 hours was found as most effective with maximized Asn, Gln and Trp with 0.849 desirability.

Organoleptic Quality, Minerals, Proteins and Amino Acids from Two Tunisian Commercial Pomegranate Fruits

2011 ◽  
Vol 7 (4) ◽  
Author(s):  
Walid Elfalleh ◽  
Nizar Tlili ◽  
Ma Ying ◽  
He Sheng-Hua ◽  
Ali Ferchichi ◽  
...  
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Amino Acid Content ◽  
Seed Proteins ◽  
Dry Weight ◽  
Essential Amino Acids ◽  
Total Sugar ◽  
Total Amino Acid ◽  
Total Amino Acid Content ◽  
Major Storage Protein

Chemicals from fresh pomegranates were established. Total sugar of juice extracts are mainly fructose (ca. 7.27 g/100 ml) and glucose (ca. 8.11 g/100 ml). Pulp minerals, expressed as mg/100g are potassium (351.26), magnesium (10.02), calcium (9.26), sodium (7.25), iron (0.15), Zinc (0.10), copper (0.08) and manganese (0.04). Juice proteins are about 7.95 g/l and dry pulp proteins are 24% on a dry-weight basis DW (Kjeldhal assays). Seed proteins are ca. 16.87% DW. Globulins (62.4 mg/g DW) and albumins (54.12 mg/g DW) are the major storage protein fractions, followed by glutelins (33.2 mg/g DW) and prolamins (18.08 mg/g DW). Seed total amino acid content is 14.45 g/100 g DW. Seeds are rich in Glu, Arg, Asp, Leu and Gly amino acids. Essential amino acids average 33.54% of total amino acid. Essential amino acids are mostly all much higher than the requirement of FAO/WHO for adults (but none for preschool children). Most other amino acids have content slightly fewer. All of these findings implied that the content of sugar, mineral and protein maybe included in pomegranate as supplements for commonly diets.

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