Response of Mungbean (cvs. Celera II-AU and Jade-AU) and Blackgram (cv. Onyx-AU) to Transient Waterlogging

Mungbean [Vigna radiata (L.) Wilczek] and blackgram [Vigna mungo (L.) Hepper] are important crops for smallholder farmers in tropical and subtropical regions. Production of both crops is affected by unexpected and increasingly frequent extreme precipitation events, which result in transient soil waterlogging. This study aimed to compare the waterlogging tolerance of mungbean and blackgram genotypes under the varying duration of waterlogging stress at germination and seedling stages. We evaluated the responses to different durations of transient waterlogging in a sandy clay loam under temperature-controlled glasshouse conditions. Waterlogging durations were 0, 1, 2, 3, 4, 5, 6, 7, and 8 days during germination and 0, 2, 4, 8, and 16 days during the seedling stage. We used two mungbean genotypes (green testa), Celera II-AU (small-seeded), and Jade-AU (large-seeded), contrasting in seed size and hypocotyl pigmentation, and a blackgram genotype (black testa), Onyx-AU. Waterlogging reduced soil redox potential, delayed or even prevented germination, decreased seedling establishment, and affected shoot and root development. In the seedlings waterlogged (WL) at 15 days after sowing (DAS), adventitious root formation and crown nodulation varied between the genotypes, and 16 days of waterlogging substantially reduced growth but did not result in plant death. Plants in soil with waterlogging for 8–16 days followed by drainage and sampling at 39 DAS had reduced shoot and root dry mass by 60–65% in mungbean and 40% in blackgram compared with continuously drained controls, due at least in part to fewer lateral roots. Soil plant analysis development (SPAD) chlorophyll content was also reduced. Onyx-AU, a blackgram genotype, was more tolerant to transient waterlogging than Jade-AU and Celera II-AU in both growth stages. Of the two mungbean genotypes, Celera II-AU had a greater seedling establishment than Jade-AU post waterlogging imposed at sowing. In contrast, Jade-AU had more plant biomass and greater recovery growth than Celera II-AU after waterlogging and recovery during the seedling stage. Both species were delayed in emergence in response to the shorter periods of transient waterlogging at germination, and with the longer waterlogging germination and emergence failed, whereas at the seedling stage both showed adaptation by the formation of adventitious roots.

Tree Canopies Influence Ground Level Atmospheric Electrical and Biogeochemical Variability

Static electric fields in the atmosphere are increasingly recognized as interacting with various organisms over several levels of biological organization. Recently, a link between atmospheric electrical variations and biogeochemical processes has been established in the context of open fields, yet biological structures like trees produce substantial alterations in atmospheric electric properties. Here, we assess whether these structural changes affect the dynamics of the electrical landscape and its relation to geochemical processes. To this end, we theoretically assess how trees alter their surrounding electric fields and empirically compare the temporal dynamics of atmospheric potential gradients, positive ions in the near-ground level atmosphere and soil electrochemical properties in an open field and under a tree. The developed model of electric fields around trees provides insight into the extent to which trees shield the underlying electric landscape, revealing that a substantial increase in atmospheric potential gradient only marginally affects the electric field under the canopy. We further show that soil electrochemical properties are tied to the temporal dynamics of positive ion in the near-ground level atmosphere, and that the presence of a tree reduces the temporal variability in both ground level positive ion concentrations and soil redox potential. This suggests that a tree can alter the temporal variability in atmospheric electricity and soil electro-chemistry, thereby likely indirectly influencing soil microorganisms and processes as well as electro-sensitive organisms that perceive and utilize atmospheric electric fields.

Azolla incorporation under flooding reduces grain cadmium accumulation by decreasing soil redox potential

Cadmium (Cd) presents severe risks to human health and environments. The present study proposed a green option to reduce bioavailable Cd. Rice pot experiments were conducted under continuous flooding with three treatments (T1: intercropping azolla with rice; T2: incorporating azolla into soil before rice transplantation; CK: no azolla). The results showed that azolla incorporation reduced soluble Cd by 37% compared with the CK treatment, which may be explained by the decreased soil redox potential (Eh) (r = 0.867, P < 0.01). The higher relative abundance of Methylobacter observed in azolla incorporation treatment may account for dissolved organic carbon increase (r = 0.694; P < 0.05), and hence decreased the Cd availability for rice. Azolla incorporation increased the abundance of Nitrospira, indicating the potentially prominent role of nitrogen mineralization in increasing rice yields. Further, lower soluble Cd decreased the expression of OsNramp5, but increased OsHMA3 levels in rice roots, which decreased Cd accumulation in grains. Through these effects, azolla incorporation decreased Cd concentrations in rice grains by 80.3% and increased the production by 13.4%. The negligible amount of Cd absorbed by azolla would not increase the risk of long-term application. Thus, intercropping azolla with early rice and incorporating azolla into soil before late rice transplantation can contribute to safe production at large scales of double rice cultivation.

Tree canopy influences ground level atmospheric electrical and biogeochemical variability

ABSTRACTStatic electric fields in the atmosphere are increasingly recognized to interact with various organisms over several levels of biological organization. Recently, a link between atmospheric electrical variations and biogeochemical processes has been established in the context of open fields, yet biological structures like trees produce substantial alterations in atmospheric electric properties. Here, we assess whether these structural changes affect the dynamics of both biogenic and abiotic electrical landscapes and their relation to geochemical processes. To this end, we theoretically assess how trees alter their surrounding electric fields and empirically compare the temporal dynamics of atmospheric potential gradients, positive ions in the near-ground level atmosphere and soil electrochemical properties in an open field and under a tree. The developed model of electric fields around trees provides insight into the extent to which trees shield underlying electric landscape, revealing that a substantial increase in atmospheric potential gradient only marginally affects the electric field under the canopy. We further show that soil electrochemical properties are tied to temporal dynamics of positive ions in the near-ground level atmosphere, and that the presence of a tree reduces the temporal variability in both ground level positive ions concentrations and soil redox potential. This suggests that a tree can have a stabilizing effect on drivers of temporal variability in atmospheric electricity and soil electro-chemistry, thereby likely indirectly influencing soil microorganisms and processes as well as electro-sensitive organisms that perceive and utilize atmospheric electric fields.

Effects of Pneumatophore Density on Methane Emissions in Mangroves

Mangroves play an important role in carbon sequestration. However, mangroves can be sources of greenhouse gas (GHG) emissions. In this study, methane (CH4) emissions and related soil properties were determined in multiple mangroves in Taiwan, including Kandelia obovata and Avicennia marina mangroves. K. obovata possess prop roots, whereas pneumatophores are found in A. marina. Our results showed that mangrove soils were significant sources of CH4 emissions, which should be accounted for in mangrove carbon budgets. In particular, CH4 emissions in the A. marina mangroves were approximately 50- to 100-fold those of the K. obovata mangroves and the adjoining mudflats. Multiple regression analyses indicated that the soil salinity and pH in K. obovata mangroves and the soil redox potential and organic content in the mudflats were the key factors affecting CH4 emissions. However, the pneumatophore density alone explained approximately 48% of the variation in CH4 emissions in the A. marina mangroves. More pneumatophores resulted in higher CH4 emissions in the A. marina mangroves. Thus, compared with the assessed soil properties, the contribution of pneumatophores to the transportation of CH4 from soil was more significant. In addition to soil properties, our results demonstrated that the root structure may also affect GHG emissions from mangroves.

Impact of fluvial flooding on potentially toxic element mobility in floodplain soils

&lt;p&gt;Climate projections suggest that rainfall events will become more frequent and intense, which may lead to more widespread flooding. Floodplains can be used to help reduce the magnitude of floods downstream by storing excess flood water, thus making them useful for flood risk management. This means that floodplains are subjected to repeated drying and rewetting, which has implications for biogeochemical cycling of chemical elements in floodplain soils.&lt;/p&gt;&lt;p&gt;Floodplains have been considered a sink for contaminants in urban catchments, where high river flows transport contaminated sediments downstream and deposit them onto the floodplain topsoil. With increasing flooding frequency and duration, floodplains may become sources of legacy pollution through desorption of contaminants into soil porewater or resuspension of particulate matter into the overlying floodwater. Therefore, flooding could re-mobilise potentially toxic elements (PTEs) such as Cadmium (Cd), Copper (Cu), Chromium (Cr), Nickel (Ni), and Lead (Pb) that are present in the floodplain soil as a result of historic deposition. Mobilising PTEs in floodplain soils may cause adverse ecological impacts for soil microorganisms, plants, and both terrestrial and aquatic fauna.&lt;/p&gt;&lt;p&gt;The mobility of PTEs from the floodplain soil can increase or decrease due to the net effect of five key processes that influence dispersion and accumulation; 1) soil redox potential for which decreases &amp;#160;can directly alter the speciation, and hence mobility, of redox sensitive PTEs (e.g. As and Cr), 2) soil pH for which an increase usually reduces the mobility of metal cations (e.g. Cd&lt;sup&gt;2+&lt;/sup&gt;, Cu&lt;sup&gt;2+&lt;/sup&gt;, Ni&lt;sup&gt;2+&lt;/sup&gt;, Pb&lt;sup&gt;2+&lt;/sup&gt;), 3) dissolved organic matter which can mobilise PTEs were strongly bound to soil particles, 4) iron (Fe) and manganese (Mn) hydroxides undergo reductive dissolution, releasing adsorbed and co-precipitated PTEs, and 5) reduction of sulphate which immobilises PTEs due to precipitation of metal sulphides.&lt;/p&gt;&lt;p&gt;We took a field-based approach; extracting soil pore waters from a floodplain downstream of a typical urban catchment in southeast England before, during and after a flooding event. During the flood, there was increased mobility of Cd and Pb, and decreased mobility for Cu and Cr, compared to the mobility before flooding. After the flood, Ni mobility increased, whereas the other PTEs had lower mobility than they had prior to the flood. We also measured explanatory variables (e.g. pH, redox, Fe and Mn) that might explain the changes in mobility of PTEs that we found. Reductive dissolution of Mn is a possible mechanism for the increased mobility of Cd and Pb and redox likely played a role in the reduced Cr mobility.&lt;/p&gt;&lt;p&gt;Flooding did not influence the mobility of all PTEs in the same way. The duration of flooding is thought to influence the mobilisation due to the length of time for key processes to take place. It is therefore difficult to predict what PTEs might be mobilised into the environment with any given flooding event, further work is required to identify which soil properties should be measured in order to improve our capability to predict how a flooding event will influence the mobility of individual PTEs in geochemically contrasting floodplain soils.&lt;/p&gt;

5-day flooding followed by 3-day drainage wet-dry alternation cycle is highly effective in reducing Cd and As content in rice

To comparatively and simultaneously investigate the reducing efficiency of the different wet-dry alternation cycles on the Cd and As absorption and transportation in rice organs, and synthesis of amino acids (AAs) in rice in two soils with different levels of Cd and As contamination, controlled pot experiments were conducted in this study. Results showed that wet-dry alternation treatments reduced Cd and As concentrations in grains by 18.8%-80% and by 77.4%-86.7% in W soil, respectively; and 76.1%-90.8% and 73.1%-80.6% in H soil, respectively. Cd and As concentrations in the soil solution were negatively and positively correlated with soil pH, respectively; but positively and negatively correlated with soil redox potential (Eh), respectively. The minimum “trade-off” values were observed in the 5 d Flooded followed by 3 d Drained treatment in both soils. The Drained treatment greatly improved AAs contents in rice organs in both soils. The changes of AAs were negatively explained more than 70% by the rachises As. Totally, the F5D3 treatment was identified as the optimal measure for simultaneously minimizing Cd and As accumulations in rice in studied soils, and water management regimes regulated the synthesis of AAs in rice organs by affecting the accumulation of As in rachises.

Spatial and Temporal Variability of Soil Redox Potential, pH and Electrical Conductivity across a Toposequence in the Savanna of West Africa

Soil redox potential is an important factor affecting soil functioning. Yet, very few agronomy studies included soil redox potential in relation to soil processes. The objective of this study was to evaluate the spatial and temporal variation in soil redox potential and to determine the soil parameters affecting its variation. Soil redox potential, soil moisture, soil temperature, pH and bulk electrical conductivity were measured in upland rice fields during two growing seasons at six positions along an upland–lowland continuum, including two positions at the upland, two at the fringe and two at the lowlands in central Côte d’Ivoire (West Africa). The measurements were made at the following soil depths: 3, 8, 20 and 35 cm. Soil redox potential varied between 500 and 700 mV at the upland positions, 400 and 700 mV at the fringe positions and 100 and 750 mV at the lowland positions, and increased with soil depth. Variations in soil redox potential were driven by soil moisture, bulk electrical conductivity and soil organic carbon. We concluded that for proper interpretation of soil redox potential, sampling protocols should systematically include soil pH, moisture and bulk electrical conductivity measurements.

Stable-Isotope-Aided Investigation of the Effect of Redox Potential on Nitrous Oxide Emissions as Affected by Water Status and N Fertilization

Soils are the dominant source of atmospheric nitrous oxide (N2O), especially agricultural soils that experience both waterlogging and intensive nitrogen fertilization. However, soil heterogeneity and the irregular occurrence of hydrological events hamper the prediction of the temporal and spatial dynamics of N2O production and transport in soils. Because soil moisture influences soil redox potential, and as soil N cycling processes are redox-sensitive, redox potential measurements could help us to better understand and predict soil N cycling and N2O emissions. Despite its importance, only a few studies have investigated the control of redox potential on N2Oemission from soils in detail. This study aimed to partition the different microbial processes involved in N2O production (nitrification and denitrification) by using redox measurements combined with isotope analysis at natural abundance and 15N-enriched. To this end, we performed long-term laboratory lysimeter experiments to mimic common agricultural irrigation and fertilization procedures. In addition, we used isotope analysis to characterize the distribution and partitioning of N2O sources and explored the 15N-N2O site preference to further constrain N2O microbial processes. We found that irrigation, saturation, and drainage induced changes in soil redox potential, which were closely related to changes in N2O emission from the soil as well as to changes in the vertical concentration profiles of dissolved N2O, nitrate (NO3−) and ammonium (NH4+). The results showed that the redox potential could be used as an indicator for NH4+, NO3−, and N2O production and consumption processes along the soil profile. For example, after a longer saturation period of unfertilized soil, the NO3− concentration was linearly correlated with the average redox values at the different depths (R2 = 0.81). During the transition from saturation to drainage, but before fertilization, the soil showed an increase in N2O emissions, which originated mainly from nitrification as indicated by the isotopic signatures of N2O (δ15N bulk, δ18O and 15N-N2O site preference). After fertilization, N2O still mainly originated from nitrification at the beginning, also indicated by high redox potential and the increase of dissolved NO3−. Denitrification mainly occurred during the last saturation period, deduced from the simultaneous 15N isotope analysis of NO3− and N2O. Our findings suggest that redox potential measurements provide suitable information for improving the prediction of soil N2O emissions and the distribution of mineral N species along the soil profile under different hydrological and fertilization regimes.