AbstractWhen retaining a sequence of auditory tones in working memory (WM), two forms of information – frequency (content) and ordinal position (structure) – have to be maintained in the brain. Here, we employed a time-resolved multivariate decoding analysis on content and structure information separately to examine their neural representations in human auditory WM. We demonstrate that content and structure are stored in a dissociated manner and show distinct characteristics. First, each tone is associated with two separate codes in parallel, characterizing its frequency and ordinal position, respectively. Second, during retention, a structural retrocue reactivates structure but not content, whereas a following white noise triggers content but not structure. Third, structure representation remains unchanged whereas content undergoes a transformation throughout memory progress. Finally, content reactivations during retention correlate with WM behavior. Overall, our results support a factorized content-structure representation in auditory WM, which might help efficient memory formation and storage by generalizing stable structure to new auditory inputs.