High-resolution structures of the actomyosin-V complex in three nucleotide states provide insights into the force generation mechanism

The molecular motor myosin undergoes a series of major structural transitions during its force-producing motor cycle. The underlying mechanism and its coupling to ATP hydrolysis and actin binding is only partially understood, mostly due to sparse structural data on actin-bound states of myosin. Here, we report 26 high-resolution cryo-EM structures of the actomyosin-V complex in the strong-ADP, rigor, and a previously unseen post-rigor transition state that binds the ATP analog AppNHp. The structures reveal a high flexibility of myosin in each state and provide valuable insights into the structural transitions of myosin-V upon ADP release and binding of AppNHp, as well as the actomyosin interface. In addition, they show how myosin is able to specifically alter the structure of F-actin. The unprecedented number of high-resolution structures of a single myosin finally enabled us to assemble a nearly complete structural model of the myosin-V motor cycle and describe the molecular principles of force production.

An Unhelmeted Motorcyclist Not Holding Both Handlebars - A Photographic Idea for Education Presented as an Initial Scoping Study

Introduction: Road safety campaigns in Australia typically have simple, hit home messages with captions overlaid on photos. The Australian stop, revive, survive campaign is well-known, with accompanying photos displaying red fatigued eyes with the words; tired eyes, yawning, driver fatigue: wake up to the signs. Otherwise, the stop, revive, survive is shown with a windy road ahead viewed through a dashboard.Objectives: The aim of this project was to create a helmet safety message utilising a photograph along with several text words alongside.Methods: A photograph taken by Stephen Hilton of a motorcyclist with no helmet, holding one handlebar, was utilised with the words;‘Helmet, Handlebars, Headlights’ overlaid above along with arrows. This safety design was shown to forty persons, in order to obtain brief feedback and comments on whether this had the potential to convey the message about the need for a helmet in particular. The comments were gathered, collated and then common themes were identified.Results and Discussion: Five emerging themes were identified that included; The message is simple and straight to the point, cultural awareness is required, the need to enforce it as law, motor cycle riders think they are invincible and finally respondents were unsure whether it would work. A word cloud was created capturing and highlighting the main words stated in larger font, while words used infrequently were displayed in smaller font.Conclusion: More than half of the respondents questioned thought the idea was simple, straight to the point and hence should work.

A Study for Locations of Accident Prone on Some Provincial Roads in Province of Kalimantan Barat

Nowadays more than one million people become victim in an accident on the roads. Some efforts have already been done to reduce globally the number accident. This paper is to show the condition of some provincial roads as location study in province of Kalimantan Barat regarding with description, characteristic, and the causes of accident. The results indicate that the human error take part dominantly around of 65% accident number, meanwhile vehicle condition and road environment contribute less than 5 %. The provincial road is dominated by the two-lane two-way facility. The finding shows the frontal collision type around of 42 % on the accident number. Number of motor cycle dominate the type of vehicle on the road. It coincides with percentage of motor cycle significant involved at an accident around of 71%. The frontal collision at location of study is more than twice compared to national data. Ratio number of accidents to length of road is located on the road segment from Ketapang to Pesaguan around of 0.884 each kilometer

IoT based Smart Motor Cycle Helmet

The main purpose of this paper is to urge the two wheeler motor cycle riders to wear a helmet. In India, most of the death cases occur due to the two wheeler accidents. In most of the cases, rider suffers head injuries, which leads to death. In order to reduce the number of accidents, this research work proposes a smart helmet, which will be very useful for the two-wheeler riders. In general, two-wheeler accidents occur due to the high consumption of alcohol by the rider. The proposed smart helmet ensures the safety of the rider, while driving on the road. The proposed model comprises of an alcohol detection, accident identification, location tracking, hands-free device, solar powered module and fall detection. The proposed smart helmet will be connected to the ignition. Henceforth, if the rider does not wear a helmet, the ignition will not be triggered to start the two-wheeler. For handling an emergency situation, GSM module is used to send an alert message to the registered mobile number.

PERANCANGAN SISTEM INFORMASI PENCARIAN KERUSAKAN SEPEDA MOTOR SISTEM ELECTRONIC FUEL IGNATION (EFI)

Have researched a sistym information about malfunction problems sitem search motor cycle which the device uses advanced methods of reasoning in expert systems . Troubelshooting on motor cycle generally used manually, by determining the symptoms theoretically derived from the experiences so that a technician and student / student often neglected problem finding time and accuracy in solving the problems of motor cycle with EFI sistem repair problems .The method used is the observation on trouble shooting to EFI sensor sistem and collect data from any symptoms of damage to television later in the evaluation of the raw data is entered into a database to an expert system by utilizing visual basic programming .The results will produce a search expert software system that can damage on EFI sensor sparepart can help the technicians and users of the motor cycle itself to recognize and deal damage that occurs EFI component sistem .