Ekstraksi Minyak Atsiri dari Bunga Mawar (Rosa hybrda L.) dengan Metode Solvent Free Microwave Extraction

To date, essential oils still play an important role in various aspects of human life. Flowers are essential oil-producing plants that still need to be further explored, of which rose petals (Rosa hybrda L.) are an option for types of flowers that have the potential to produce economical essential oils. In this study, solvent-free microwave extraction (SFME) was used as an essential oil extraction method, which is considered safe because it does not use heat energy and chemical solvents which can be considered to trigger a decrease in the quality of the oil extract. The optimum microwave power in this study is 560 Watt with a yield of 0.0124%. The difference in the value of the feed intake ratio to the volume of the distiller (F/D) influences yield, where the F/D value of 0.15 g/mL gives the highest yield value of 0.0145%. The result of the Gas Chromatography Mass Spectrometry (GCMS) analysis shows rose flower essential oil extract provided a profile of 12 compounds, of which three dominant compounds are β-phenylethyl acetate, 2-isopropyl-5-methyl-9-methylene-bicyclo-1-decene, and nonadecane. Keywords: Extraction, rose flower petals, solvent-free microwave extraction (SFME), essential oils Hingga saat ini, minyak atsiri masih memegang peranan penting untuk berbagai aspek kehidupan manusia. Bunga-bungaan merupakan tanaman penghasil minyak atsiri yang masih perlu dieksplorasi lebih lanjut, yang mana mahkota bunga mawar (Rosa hybrda L.) adalah satu opsi jenis bunga yang berpotensi sebagai penghasil minyak atsiri bernilai ekonomis. Pada penelitian ini digunakan solvent-free microwave extraction (SFME) sebagai metode ekstraksi minyak atsiri yang dinilai aman karena tidak menggunakan energi panas dan bahan pelarut kimia yang dapat dianggap memicu penurunan kualitas ekstrak minyak. Daya microwave optimum pada penelitian ini adalah 560 Watt dengan perolehan yield sebesar 0,0124%. Perbedaan nilai rasio feed masuk terhadap volume distiler (F/D) memberikan pengaruh terhadap hasil rendemen, yang mana nilai F/D 0,15 g/mL memberikan nilai rendemen tertinggi sebesar 0,0145%. Hasil analisis Gas Chromatography Mass Spectrometry (GCMS) menunjukkan bahwa ekstrak minyak atsiri bunga mawar memberikan profil 12 senyawa dengan tiga senyawa dominan yaitu β-phenylethyl acetate, 2-isopropyl-5-methyl-9-methylene-bicyclo-1-decene, dan nonadecane. Kata kunci: Ekstraksi, mahkota bunga mawar, solvent-free microwave extraction

Rose (Rosa hybrida) Ethylene Responsive Factor 3 Promotes Rose Flower Senescence via Direct Activation of The Abscisic Acid Synthesis-Related 9-CIS-EPOXYCAROTENOID DIOXYGENASE Gene

During plant senescence, energy and nutrients are transferred to young leaves, fruits or seeds. However, senescence reduces flower quality, which leads to huge economic losses in flower production. Ethylene is an important factor affecting the quality of cut roses during transportation and storage. Ethylene-responsive factors (ERFs) are key nodes in ethylene signaling, but the molecular mechanism underlying ERFs regulated flower senescence is not well understood. We addressed this issue in the present study by focusing on RhERF3 from Rosa hybrida, an ERF identified in a previous transcriptome analysis of ethylene-treated rose flowers. Expression of RhERF3 was strongly induced by ethylene during rose flower senescence. Transient silencing of RhERF3 delayed flower senescence whereas overexpression (OE) accelerated the process. RNA sequencing analysis of RhERF3 OE and pSuper vector control samples identified 13,214 differentially expressed genes that were mostly related to metabolic process and plant hormone signal transduction. Transient activation and yeast one hybrid assays demonstrated that RhERF3 directly bound the promoter of the 9-cis-epoxycarotenoid dioxygenase (RhNCED1) gene and activated gene expression. Thus, a RhERF3/RhNCED1 axis accelerates rose flower senescence.

The Effect of Lavender and Rose Aromatherapy on the Intensity of Active Phase Childbirth Pain in the Manyak Payed Community Health Center, Aceh Tamiang Regency, Indonesia

BACKGROUND: Most postpartum mothers experience pain. Childbirth pain can cause anxiety, fear, tension, and stress. To increase comfort and reduce pain in maternity can be done using non-pharmacological methods by giving aromatherapy. AIM: The aim of the study was to analyze the effect of lavender and rose flower aromatherapy on the intensity of active phase childbirth pain in pregnant women in Manyak Payed Community Health Center, Aceh Tamiang Regency, Indonesia METHODS: A quasi-experimental design with a prospective cohort study with post-test control group design, which was conducted in the Manyak Payed Community Health Center working area from September to December 2019. A total of 54 women giving birth using accidental sampling techniques were selected as samples and divided into three groups. Each group numbered 18 people. In this sample using inclusion and exclusion criteria, data analysis uses univariable and bivariable using Kruskal–Wallis H test and Mann–Whitney test. RESULTS: The results obtained mean childbirth pain in the lavender group by 18.83, rose flower group by 26.21, and the control group by 37.06. The results of the crucial Willis H test show that there are differences ineffectiveness in the control group and the treatment group with the Sig. 0.001 (<0.05). In further tests of the post hoc test using the Mann–Whitney test, it was found that there was no difference in the effectiveness of the intensity of labor pain in the active phase I with a sig. 0.90 (p > 0.05). Lavender and rose flower groups were equally effective in the intensity of childbirth pain. CONCLUSION: There is a difference between lavender and rose flower aromatherapy on the intensity of pain in the association. Lavender and rose flower aromatherapy treatments are equally influential on the intensity of labor pain in the first phase of the active phase in labor. Lavender and rose flower groups were equally effective in the intensity of childbirth pain.