Phosphorus Retention of a Permeable Reactive Barrier Surpassed by an Unvegetated Artificial Pond

An artificial pond bisected by a phosphorus (P) retentive permeable reactive barrier (PRB) alongside Forrest Highway, Coolup, Western Australia was designed to remove P from farmland runoff. The pond bed was made of subsoil and road construction materials likely to have a relatively high P sorption capacity, and there was no vegetation in the bed of the pond. Flow through the pond was intercepted by the PRB, constructed from a mixture of sand, coarse crushed limestone, and bauxite residue (with 10% phospho-gypsum). The effectiveness of P removal and the impact of the PRB was measured by comparing the concentration of contaminants immediately either side of the PRB with established standards, and against background levels in runoff from surrounding farmland. Using coarse limestone to increase flow through the PRB failed where permeability was insufficient to avoid overtopping of the PRB and the wall had to be lowered to allow by-pass and avoid collapse. The PRB was effective in removing total P (TP); however, the influent TP concentration was low (mean 0.19 mg L -1 ) because most P entering from farmland was retained in the shallow pond upstream of the PRB. Despite this, TP removal by the PRB was 53% (2009–2012). Occasionally, in spring when the pond was stagnant and anaerobic, P was released from the PRB. This minor P release coincided with a minor release of iron, consistent with anaerobic conditions found in the PRB. Although not designed to do so, the shallow pond upstream of the PRB reduced the TP concentration from farmland by 85% (mean 1.26 mg L -1 down to 0.19 mg L -1 ), mainly by reducing filterable reactive P concentration. Some elements (arsenic, cobalt, conductivity, fluoride, manganese, molybdenum, pH, selenium, uranium and vanadium) were increased by flow through the PRB, but were low relative to surrounding waters and environmental standards

Optimization and Application of direct PCR in community metabarcoding

Direct PCR allows the amplification of DNA from animal or plant tissue samples without the need for DNA extraction and purification steps. For this procedure, dry tissue is homogenized, dissolved in water and subsequently amplified, thus, its successful application largely depends on the absence of PCR inhibitors. Although this method has been successfully applied in barcoding approaches of invertebrates, it has not yet been attempted in metabarcoding approaches. We used nonbiting midges (Diptera: Chironomidae) to test if amplicons produced by direct PCR could be used for next-generation sequencing. To access whether direct PCR is applicable for a variety of chironomid species, we tested 236 adult specimens randomly selected from emergence traps of an artificial pond mesocosm. We used ground tissue, corresponding to 0.1% of the specimens’ biomass, and a direct PCR protocol following Wong et al. (2014) for amplification. In total, 98 % of the samples were successfully amplified and we found a diverse community comprised of 20 different genera. In order to compare direct PCR and ´traditional´ DNA isolation-PCR, we created mock communities (14 species) and used both approaches for the amplification of a 421 bp COI fragment. After a second PCR for indexing and adapter ligation, samples were sequenced on an Illumina sequencer. We found only slightly lower recovery rates for mock communities with the direct PCR approach compared to traditional protocols. These recovery rates were further improved for both methods when an equal biomass (ca. 0.006 mg) of chironomid specimens was used. With our approach, it was possible to detect species which constituted only 1% of the entire biomass of a sample. Generally, direct PCR did not have a large effect on sequence read abundance. However, read abundance varied strongly between species. We are currently investigating whether this was caused by primer bias or an artifact of differently degraded tissue. This study is a proof of principle that the amplicons produced by direct PCR can be used for next-generation sequencing, with possible applications for future biomonitoring projects and portable laboratory technologies. We are currently using this technique to monitor a large-scale chironomid community experiment (artificial pond mesocosm facility) covering weekly samples taken over two summer half-years.

Vertebrate species richness at littoral beaver lodges in a temperate artificial pond

The North American beaver Castor canadensis is widely recognized for its ability to modify freshwater habitats and facilitate changes in community composition. However, the seasonal composition of terrestrial wildlife at littoral beaver lodges remains poorly described, even though these structures are distinctive semi-permanent features of the terrestrial-aquatic interface and thus important resources for wildlife. Over 17 months, we used camera trapping, weather data, and satellite vegetation data to determine how vertebrate species richness and seasonal changes in community composition are associated with beaver activity and beaver lodges in a temperate artificial pond. Our results indicate clear changes in the composition of lodge visitors across seasons. Moreover, species richness was not strongly associated with beaver activity, vegetative condition, or weather condition. Littoral beaver lodges are likely important foraging sites for a wide range of taxa throughout the year. Our findings highlight the importance of beaver lodges in facilitating seasonal interactions and variation in species composition. We hope our work can be used as a baseline to investigate the importance of beaver structures in promoting diversity at the terrestrial-aquatic interface.

First records of two alien land flatworms (Tricladida, Geoplanidae) from Northeastern Italy

During a period of intense rainfall (May 2019), several specimens of land flatworms were collected from a private garden in Palazzolo dello Stella (Friuli Venezia Giulia, Udine, Italy: 45°47’40.5”N, 13°05’17.2”E). Planarians were found both in a cultivated part of the garden and in a part covered with gravel and with trees and shrubs (Pyracantha sp., Olea europaea, Pyrus communis). The animals were observed under branches, stones, tufa blocks, and pots close to a small artificial pond, but also in other parts of the garden, as well as inside buildings.

Kebutuhan Air Harian Masyarakat Desa Jada Bahrin Kabupaten Bangka

Jada Bahrin village produces vegetables, rubber, pepper, and pineapple. The people in this village work as a farmer, fisherman, trader, and entrepreneur. They face a big problem to get water in dry season. They only rely on artificial pond to collect and distribute the water to the people. The goals of this study is to know the average daily water demand at present and the prediction of water needs for the next 10 years as well as the factors that influence the water needs in Jada Bahrin. Data for analysis were gathered by distributing questionnaires to respondents. Based on the analysis, the average daily water demand of each person in Jada Bahrin Village was 107.83 liters/person/day (Ipoph) and the prediction of the total water needs of Jada Bahrin Village in the next 10 years (2028) was 2.43 liters/second. Factors affecting the need for clean water were the type of work, the level of income and the influence of climate

An Efficient Method for Water Treatment of Artificial Ponds in Jordan Valley Based on Photovoltaic Pumping System

Jordan Valley area suffers from a lack of water because of the current political situation. Therefore, water distribution is being done on a periodic basis and farmers need to store water in artificial ponds on site so as to be able to irrigate their field during the anonymous days that water is not available from the main supply. However, artificial ponds may affect the environment negatively due to the plants that live in it such as algae, which attracts mosquitos and causes a bad smell. Thus, in this paper, a simple and low-cost photovoltaic based pumping system is proposed to inject a chemical material in the water of the artificial pond to get rid of algae. The proposed system consists of a pump that is powered by a photovoltaic module and pumps the proposed chemical material in the artificial pond using a rotary nozzle that is fixed on a pipe around the ponds. The system is affordable and reduces the production of the unwanted plants. As a result, the proposed system reduces chemical oxygen demand value, which is considered the main cause of algae blooming, from 7200 mg/L to 95 mg/L. The proposed product is powered by a 50 W foldable solar panel and it costs about 213 USD.