Inlay butterfly versus underlay cartilage tympanoplasty

Background Tympanic membrane grafting is one of the most common otological procedures. Underlay technique of tympanoplasty was described by Shea in 1960. Utech in 1959 introduce the cartilage in middle ear surgery. The search for an ideal graft material and technique for tympanoplasty was adopted by numerous contributions from surgeons all over the world. Professor Roland Eavey in 1998 introduced a transcanal inlay technique which offers advantages of surgical ease and speed as well as patient comfort. Results This study included 46 patients; 23 patients had inlay butterfly myringoplasty (group A), and 23 patients had underlay cartilage tympanoplasty. In group A, 65 % of the patients had a completely healed tympanic membrane postoperatively. Mean AB gap closure was 3.94 db. In underlay group B, 82.6% of the patients had a completely healed tympanic membrane postoperatively. Mean AB gap closure was 4.7 db. These outcomes show no statistically significant difference between both groups in terms of graft take and hearing improvement (p > 0.1). Conclusions Inlay butterfly myringoplasty is an easy, reliable, and time saving procedure that should be possible as a choice to underlay procedure. Results are comparable with underlay technique in terms of graft take rate and hearing improvement. Procedure is better regarding diminishing operative time, postoperative pain, and duration before resuming usual activities.

Sustained Released of Bioactive Mesenchymal Stromal Cell-Derived Extracellular Vesicles from 3D-Printed Gelatin Methacrylate Hydrogels

Extracellular vesicles (EVs) represent an emerging class of therapeutics with significant potential and broad applicability. However, a general limitation is their rapid clearance after administration. Thus, methods to enable sustained EV release are of great potential value. Here, we demonstrate that EVs from mesenchymal stem/stromal cells (MSCs) can be incorporated into 3D-printed gelatin methacrylate (GelMA) hydrogel bioink, and that the initial burst release of EVs can be reduced by increasing the concentration of crosslinker during gelation. Further, the data show that MSC EV bioactivity in an endothelial gap closure assay is retained after the 3D printing and photocrosslinking processes. Our group previously showed that MSC EV bioactivity in this assay correlates with pro-angiogenic bioactivity in vivo, thus these results indicate therapeutic potential of MSC EV-laden GelMA bioinks.

Minding the Design Reality Gap

This paper focuses on evaluating an information and communication technology (ICT) intervention promoted as a pro-poor telecentre initiative in rural Ghana. Our evaluative tool is the Design Reality Gap (DRG) framework used to analyse the Community Information Centre (CIC) initiative in Ghana. Data were collected through a qualitative multi-site case study. By tracing the linkages between the investment and outcomes, we found a worrying trend of failed implementations and sustainability, although implementers did sustain efforts at planning new initiatives. Based on the findings, we argue that the CIC initiative in Ghana is a failing ICT intervention. We also found that the tailored DRG approach allowed us to tease out the nuances that account for the CICs' status. We conclude by proposing gap closure measures for the failing intervention. This paper contributes to ICT evaluations by demonstrating the utility of the DRG framework in evaluating one of the most significant pro-poor ICT initiatives in lower-to-middle-income communities: telecentres. This research also contributes to the current ICT literature by enhancing our current knowledge about publicly accessible ICT facilities in an under-investigated setting, and further offers an approach to telecentre evaluations in similar contexts inspired by the DRG model.

A retrospective study on middle ear risk indices in analysing the post-operative outcome following tympanoplasty

<p class="abstract"><strong>Background:</strong> Chronic otitis media is a highly prevalent disease of middle ear. As tympanoplasty plays an important role in management of otitis media it is important to predict the outcome of surgery and give proper counselling to the patient. The objective of this study was to evaluate the effectiveness of Middle ear risk index (MERI) in determining postoperative graft uptake and postoperative air bone gap closure following tympanoplasty.</p><p class="abstract"><strong>Methods:</strong> This was a retrospective study conducted to find the correlation between MERI and the result of tympanoplasty outcome and post-operative hearing gain. Ninety-six subjects who underwent tympanoplasty for chronic otitis media were included in the study. MERI score was calculated and was compared with postoperative graft uptake and audiogram. </p><p class="abstract"><strong>Results:</strong> In this study there was no statistical difference in graft uptake between the MERI categories. There was statistically significant decrease in postoperative air bone gap closure in both mild and moderate categories. Other prognostic factors associated with a favourable outcome were healthy middle ear mucosa and absence of smoking.</p><p class="abstract"><strong>Conclusions:</strong> MERI did not have a direct impact on the outcome of surgery in terms of graft uptake and hearing results. However certain factors like Belluci’s criteria and smoking had an impact on the outcome. Hence MERI can be used for pre-operative counselling of the patients.</p>

Intestinal Obstruction Due to Titanium Tack after TAPP Inguinal Hernia Repair

Background: Small bowel obstruction (SBO) after transabdominal preperitoneal (TAPP) laparoscopic inguinal hernia repair is a rare complication. It is generally associated to a failure of the peritoneal gap closure with intestinal loops passing through the gap. Case Presentation: We report a case of SBO secondary to titanium tack after 2 weeks of laparoscopic TAPP repair managed by laparoscopic approach, and we update some considerations about this unfortunate complication. Conclusion: SBO due to mechanical fixation devices utilized in laparoscopic TAPP repairs is infrequent. In case of closing of peritoneal gap with tacks, those with low exposition profile and absorbable should be preferred.

Analysis of a DKA protocol: Laboratory tests and outcomes

Most hospitals use protocols to manage diabetic ketoacidosis using laboratory criteria to monitor the response to insulin and fluid therapy. This study analyzed information collected prospectively on a group of patients with diabetic ketoacidosis to review outcomes and management details. The study included 37 patients with a mean age of 38.1 ± 18.5 years. The initial blood glucose was 546.4 ± 296.3 mg/dL. The initial anion gap was 31.8 ± 7.8 meq/L. The mean time to anion gap closure twice was 19.2 ± 12.8 hours. The mean fluid administered until anion gap closure was 3694.9 ± 2484.6 mL. The insulin dose during the first day of management was 69.5 ± 49.9 units; the mean number of point-of-care glucose levels during the first 24 hours was 21.2 ± 6. The number of basic metabolic panels collected during hospital management was 12.2 ± 13.7. The mean ICU length of stay was 2.5 ± 3.8 days with no mortality. This study indicates that patients with diabetic ketoacidosis had a large number of basic metabolic panel tests and a large number of point of care glucose measurements using this protocol. This protocol needs review to determine whether or not the number of tests can be reduced, and transfer out of the ICU can occur more quickly. Key words: Diabetic ketoacidosis, glucose levels, anion gap, basic metabolic panels, outcomes

To compare the efficacy of Titanium versus Teflon piston on hearing outcome in terms of Air conduction gain and Air Bone Gap closure in LASER Stapedotomy – A retrospective study

Aim: To determine the efficacy of Titanium versus Teflon piston on hearing outcome in terms of Air Conduction Threshold (ACT) gain and Air Bone Gap (ABG) closure in patients undergoing LASER Stapedotomy. Methods: This retrospective study was conducted at the department of ENT and Head and Neck Surgery, AIIMS Patna. 40 patients underwent LASER stapedotomy. Both Teflon (0.4mm and 0.6mm D, Grace Medical Devices) and Titanium (0.4mm D, Kurz Medical Devices) pistons were inserted randomly. Crimping of titanium Kurz piston was not required due to its unique design which fits on to the incus long process like a clip whereas all the Teflon pistons required crimping.Titanium (Kurz Medical Devices) pistons are costlier in comparison to Teflon (Grace Medical Devices) pistons. Preoperative and 3 monthly postoperative Pure Tone Audiograms (PTA) wereobtained. ACT (Air conduction threshold) gain and ABG (Air Bone Gap) closure were measured.Data was analyzed with IBM SPSS Statistics software Version 20. Descriptive analysis,paired and unpaired t-tests were applied. P-value of <0.05 at 95% confidence levels was considered statistically significant. Results: 40 patients (M: F: 3:7) underwent LASER stapedotomy.Titanium piston was utilized in 11and Teflon in 29 patients. Patients with titanium piston experienced a mean ACT gain of 21.51±05.42dBand mean ABG closure of 12.27±05.18 dB. Those with Teflon piston gained a meanACT gain of 18.54±04.43 dB and mean ABG closure of11.96±06.12 dB.Both groups experienced statistically significant hearing gain (p <0.05).Both groups were however comparable and the difference between these parameters was statistically insignificant (p>0.05).Conclusion: Both titanium and Teflon pistons are equally efficacious in improving postoperative hearing gain in LASER stapedotomy patients in terms of ACT gain and ABG closure. Titanium Kurz pistons do not require crimping making it easier to use in comparison to the Teflon pistons which needs to be crimped every time though cost may be a limiting factor in the usage of Titanium pistons.Both types of pistons can be satisfactorily employed in this surgical procedure if we consider the hearing gain post operatively. Study design: A retrospective observational study Keywords: Otosclerosis, Teflon, Titanium, Air Conduction Threshold (ACT), Air Bone Gap (ABG) closure

Gap analysis of emergency department pharmacy services within a multistate, multihospital integrated health system

Purpose The purpose of this project was to identify current emergency medicine pharmacist (EMP) practices at each site and create a plan to integrate, align, and optimize pharmacy services across the health system with established American Society of Health-System Pharmacists (ASHP) best practices for EMPs. Summary Initially, a review was performed of the literature and guidelines from professional organizations relating to EMPs. A survey was distributed across the health system to assess EMP services at each site, and survey results were used to conduct a gap analysis, comparing current practices to established ASHP best practices. The survey identified unique components of each site, including the patient population served and EMP coverage and responsibilities. To prioritize, design, and execute the gap closure plan, a systemwide EMP workgroup was created. The workgroup formulated a toolkit to provide pharmacy leaders, pharmacy informatics, and EMPs resources to facilitate alignment on the prioritized areas. Conclusion This project successfully identified gaps in EMP services and alignment with best practices across the health system. Through prioritization of essential EMP responsibilities, workflow standardization, and EHR optimization, a gap closure plan was formulated to align with ASHP best practices.

Hindbrain neuropore tissue geometry determines asymmetric cell-mediated closure dynamics in mouse embryos

Gap closure is a common morphogenetic process. In mammals, failure to close the embryonic hindbrain neuropore (HNP) gap causes fatal anencephaly. We observed that surface ectoderm cells surrounding the mouse HNP assemble high-tension actomyosin purse strings at their leading edge and establish the initial contacts across the embryonic midline. Fibronectin and laminin are present, and tensin 1 accumulates in focal adhesion-like puncta at this leading edge. The HNP gap closes asymmetrically, faster from its rostral than caudal end, while maintaining an elongated aspect ratio. Cell-based physical modeling identifies two closure mechanisms sufficient to account for tissue-level HNP closure dynamics: purse-string contraction and directional cell motion implemented through active crawling. Combining both closure mechanisms hastens gap closure and produces a constant rate of gap shortening. Purse-string contraction reduces, whereas crawling increases gap aspect ratio, and their combination maintains it. Closure rate asymmetry can be explained by asymmetric embryo tissue geometry, namely a narrower rostral gap apex, whereas biomechanical tension inferred from laser ablation is equivalent at the gaps’ rostral and caudal closure points. At the cellular level, the physical model predicts rearrangements of cells at the HNP rostral and caudal extremes as the gap shortens. These behaviors are reproducibly live imaged in mouse embryos. Thus, mammalian embryos coordinate cellular- and tissue-level mechanics to achieve this critical gap closure event.