Olive Fruit Extracts Supplement Improve Antioxidant Capacity via Altering Colonic Microbiota Composition in Mice

Oxidative stress, one of the most common biological dysfunctions, is usually associated with pathological conditions and multiple diseases in humans and animals. Chinese olive fruit (Canarium album L.) extracts (OE) are natural plant extracts rich in polyphenols (such as hydroxytyrosol, HT) and with antioxidant, anti-hyperlipidemia, and anti-inflammatory potentials. This study was conducted to investigate the antioxidant capacity of OE supplementation and its related molecular mechanism in mice. Mice (25.46 ± 1.65 g) were treated with 100 mg/kg body weight (BW) OE or saline solution for 4 weeks, and then the antioxidant and anti-inflammatory capacities of mice were examined. The results showed that OE supplement significantly increased the serum antioxidative enzyme activities of total antioxidant activity (T-AOC), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase and decreased the serum malondialdehyde (MDA) level, indicating that OE treatment enhanced the antioxidant capacity in mice. qPCR results showed that the transcriptional expression of antioxidant SOD1, CAT, Gpx1, and Gpx2 were significantly down-regulated in the small intestine (jejunum and ileum) after OE administration. Meanwhile, OE treatment significantly decreased the T-AOC and increased the MDA level in the small intestine. Furthermore, OE administration dramatically reduced the mRNA expression of pro-inflammatory cytokines (TNF-α and IL-1β), which confirmed its antioxidant and anti-inflammatory capacities with OE administration. Using amplicon sequencing technology, 16S rRNA sequencing results showed that OE supplement significantly increased the colonic Firmicutes/Bacteroidetes ratio, which also had a negative correlation with the serum MDA level and positively correlated with serum GSH-Px activity through Pearson correlation analysis. Besides that, Alloprevotella was negatively correlated with serum T-AOC. Colidextribacter was positively correlated with serum MDA and negatively correlated with serum T-AOC, SOD, and GSH-Px levels. In summary, this study showed that treatment with 100 mg/kg BW polyphenol-rich OE could alter colonic microbiota community, which was strongly associated with improved antioxidant capacity in mice.

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Gum Arabic (Acacia Senegal) Augmented Total Antioxidant Capacity and Reduced C-Reactive Protein among Haemodialysis Patients in Phase II Trial

International Journal of Nephrology ◽

10.1155/2020/7214673 ◽

2020 ◽

Vol 2020 ◽

pp. 1-7 ◽

Cited By ~ 1

Author(s):

Nour Elkhair Ali ◽

Lamis AbdelGadir Kaddam ◽

Suad Yousif Alkarib ◽

Babikir Gabir Kaballo ◽

Sami Ahmed Khalid ◽

...

Keyword(s):

Oxidative Stress ◽

Antioxidant Capacity ◽

Total Antioxidant Capacity ◽

Gum Arabic ◽

Oxidative Stress Marker ◽

C Reactive Protein ◽

Reactive Protein ◽

Total Antioxidant ◽

Anti Inflammatory ◽

End Stage

Background. Oxidative processes might increase in patients with end-stage renal disease (ESRD) according to the current literature. Oxidative stress (OS) is a risk factor of atherosclerosis and cardiovascular complications, which are major causes of mortality among ESRD patients. Haemodialysis (HD) is life-saving procedure, nevertheless it is an active chronic inflammatory status that could augment cardiovascular disease and increase mortality. Gum Arabic (GA) has been claimed to act as an antioxidant and anti-inflammatory agent in experimental studies and clinical trials. Therefore, we assumed GA supplementation among haemodialysis patients would reduce oxidative stress and consequently reduce the state of chronic inflammatory activation associated with haemodialysis. Methods. Forty end-stage renal failure (ESRF) patients aged 18–80 years who were on regular haemodialysis in Arif Renal Center, Omdurman, Sudan, were recruited. All recruited patients met the inclusion criteria and signed informed consent prior to enrolment. The patients received 30 g/day of GA for 12 weeks. C-reactive protein (CRP) and complete blood count (CBC) were measured as baseline and monthly. Total antioxidant capacity (TAC) and oxidative stress marker malondialdehyde (MDA) levels were measured before and after GA intake. Ethical approval from the National Medicines and Poisons Board was obtained. Results. Gum Arabic significantly augmented total antioxidant capacity level (P<0.001) (95% CI, 0.408–0.625) and also attenuated oxidative marker MDA and C-reactive protein (P<0.001). Conclusions. GA has revealed potent antioxidative and anti-inflammatory properties in haemodialysis patients. Oral digestion of GA (30 g/day) decreased oxidative stress and inflammatory markers among haemodialysis patients. Trial registration. ClinicalTrials.gov Identifier: NCT03214692, registered 11 July 2017 (prospective registration).

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ANTIOXIDANT AND ANTI-INFLAMMATORY ACTIVITIES OF RUBUS FRUTICOSUS AND ZIZYPHUS VULGARIS METHANOL EXTRACTS

International Journal of Pharmacy and Pharmaceutical Sciences ◽

10.22159/ijpps.2017v9i2.14374 ◽

2017 ◽

Vol 9 (2) ◽

pp. 69

Author(s):

Asma Meziti ◽

Hamama Bouriche ◽

Meziti Hichem ◽

Seoussen Kada ◽

Abderrahmane Senator ◽

...

Keyword(s):

Oral Administration ◽

Antioxidant Capacity ◽

Vascular Permeability ◽

Dna Cleavage ◽

Total Antioxidant Capacity ◽

Rubus Fruticosus ◽

Total Antioxidant ◽

Anti Inflammatory

Objective: This report is an attempt to study the phenolic composition of Rubus fruticosus (RFE) and Zizyphus vulgaris (ZVE) methanol extracts and evaluate their antioxidant and anti-inflammatory effects in-vitro and in-vivo.Methods: Total phenolic and total flavonoids contents of extracts were determined by spectrophotometric methods. Phenolic compounds were identified by HPLC-TOF/MS. The antioxidant activities were evaluated in vitro using DPPH, ABTS and FRAP assays. The effect of RFE and ZVE on DNA cleavage induced by H2O2 UV-photolysis was also investigated. The antioxidant effect of RFE and ZVE was tested in vivo using the blood total antioxidant capacity test in mice. On the other hand, the anti-inflammatory activity was assessed in vivo using two models of acute inflammation ear edema and vascular permeability.Results: The phytochemical analysis of these extracts showed that RFE possesses higher polyphenolic and flavonoid content than ZVE. in the same way RFE exerted the highest antioxidant capacity with IC 50 value of 14 µg/ml in DPPH assay, 1.58 mmol of Trolox E/mg extract and 3.39 of mmol FesO4/mg extract in ABTS, and FRAP assay respectively. The studied extracts showed a concentration-dependent protective effect on DNA cleavage induced by H2O2 UV-photolysis. The daily oral administration of 200 mg/kg of RFE or ZVE during three weeks showed an improvement of the blood total antioxidant capacity; the HT50 values were151.45 min and 146.72 min for the groups treated with RFE and ZVE, respectively versus 122.5 min for the control group. The topical application of 2 mg/ear of RFE inhibited the croton oil-induced ear edema by 75.72%, while the inhibition exerted by ZVE was 64.24%. These inhibitions were higher than that of indomethacin, used as a reference. Moreover, the oral administration of 400 mg/kg of RFE inhibited significantly (33.57%) acetic acid induced vascular permeability in mice. However, this effect was lower than this of indomethacin. The inhibition effect exerted by ZVE was not significant.Conclusion: The results obtained in this investigation showed that RFE possesses strong antioxidant and anti-inflammatory potential in comparison with ZVE, which may be attributed to the presence of polyphenolic phytoconstituents.

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Xylanase and Fermented Polysaccharide of Hericium caputmedusae Reduce Pathogenic Infection of Broilers by Improving Antioxidant and Anti-Inflammatory Properties

Oxidative Medicine and Cellular Longevity ◽

10.1155/2018/4296985 ◽

2018 ◽

Vol 2018 ◽

pp. 1-11 ◽

Cited By ~ 5

Author(s):

Sitong Zhang ◽

Chunfeng Wang ◽

Yang Sun ◽

Gang Wang ◽

Huan Chen ◽

...

Keyword(s):

Antioxidant Capacity ◽

Bacterial Pathogens ◽

Interleukin 1 ◽

Serum Levels ◽

Control Group ◽

Coagulase Negative Staphylococci ◽

Basic Diet ◽

Total Antioxidant ◽

Anti Inflammatory ◽

Pathogenic Infection

Background. Pathogenic infection in broilers has become an important issue in the development of poultry industry. Xylooligosaccharides released from xylan via xylanase and fermented polysaccharide of Hericium caputmedusae (FPHC) have antimicrobial potential against many pathogens. Objective. We aimed to explore the effects of xylanase and FPHC on pathogenic infection in the broilers (Gallus gallus domesticus). Methods. Three hundred and thirty 21-day male broilers were assigned into four groups: control group (CG, basic diet), xylanase group (XG, basic diet + xylanase), FPHC group (HG, basic diet + FPHC), and XHG group (basic diet + xylanase + FPHC). Average daily feed intake (ADFI) and daily gain (ADG) were measured. Microflora from broiler feces was analyzed using 16S rRNA sequencing. Serum tumor necrosis factor- (TNF-) α, interleukin-1β (IL-1β), IL-1 receptor antagonist (IL-1ra), IL-10, total antioxidant capacity (T-AOC), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and malondialdehyde (MDA) contents were detected using kits. The variables were compared using the Student t-test between two groups. Results. Microbiological investigations showed that 75% of broilers were affected by bacterial pathogens in the CG group, most notably by coagulase-negative staphylococci. Comparatively, 15%, 26%, and 5% of broilers were affected by bacterial pathogens in the XG, HG, and XHG groups, respectively. Xylanase and FPHC treatment increased the ratio of ADG to ADFI and antioxidant capacity by increasing the levels of T-AOC, SOD, and GSH-Px and reducing the levels of MDA (P<0.05). Xylanase and FPHC treatment improved anti-inflammatory capacity by increasing serum levels of IL-1ra and IL-10 and reducing the levels of IL-1β and TNF-α. On the other hand, the treatment increased probiotic concentration of Bacillus licheniformis, Bacillus subtilis, and Lactobacillus plantarum (P<0.05), which were also proved in cell culture. Conclusions. Xylanase and FPHC ameliorate pathogen infection by increasing antioxidant and anti-inflammatory activities of broilers via the increase of probiotics.

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Phytochemical Profile, Antioxidant and Anti-Inflammatory Activities of Ephedra alata Decne Growing in South Algeria

10.20944/preprints202108.0296.v1 ◽

2021 ◽

Author(s):

Bachir Benarba ◽

Ouafae Douad ◽

Chaima Gadoum ◽

Khadidja Belhouala ◽

Soumicha Mahdjour

Keyword(s):

Antioxidant Capacity ◽

Aqueous Extract ◽

Total Antioxidant Capacity ◽

Methanolic Extract ◽

Reducing Power ◽

Egg Albumin ◽

Human Red Blood Cells ◽

Methanolic Extracts ◽

Total Antioxidant ◽

Anti Inflammatory

Ephedra alata Decne. (Ephedraceae) is a medicinal species commonly used to treat cancers, respiratory diseases, fever, and hypertension. The present study aimed to establish a phytochemical profile, evaluate the antioxidant potential and estimate the anti-inflammatory activities of .. Aqueous and methanolic extracts of E. alata aerial parts were phytochemically investigated using standard methods. DPPH, phosphomolybdenum total antioxidant capacity and reducing power assays were used to evaluate the antioxidant activity. Antioxidant activity was determined using total antioxidant capacity, the scavenging activity of the stable DPPH free radical and ferric reducing antioxidant power assays. The anti-inflammatory activity was determined using egg albumin membrane denaturation and human red blood cells membrane stabilizing assays. The anti-inflammatory potential of E. alata extracts was evaluated using human red blood cells membrane stabilization, egg albumin and BSA albumin denaturation assays. Quinones, anthraquinones, steroids, phytosteroids, phenols, terpenoids, flavonoids, saponins, glycosides, Cardiac glycosides, reducing sugars and anthocyanins were present in the E. alata’s aqueous extract, in addition to coumarins and proteins in the methanolic extract. The highest total phenolic and flavonoid content was recorded in the aqueous extract with 8.66 ±0.09 mg GA/g and 248.04 ±1.47 mg Q/g, respectively. On the other hand, E. alata methanolic extract had the highest tannin content of 62.12 ±0.10 mg C/g. The best radical scavenging activity (IC50 = 4.63±0.00 mg/ml) and total antioxidant capacity were exhibited by the E. alata aqueous (7.35±0.12 mg/ml AAE), whereas the methanolic extract possessed the highest reducing power activity (1.81±0.00 mg AAE/ml). Regarding the anti-inflammatory activities, E. alata methanolic extract exerted the highest HRBC stabilization of 34.72 ±0.08% whereas the aqueous extract exhibited the highest bovine serum and Egg albumin denaturation inhibition of 99.22 ±0.02% and 73.31 ±0.90, respectively. Taken together, our results suggest that E. alata aerial parts aqueous and methanolic extracts can be utilized as future antioxidants and anti-inflammatory ethnomedicines owing to their rich bioactive molecules content.

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Proximate Composition, In vitro Antioxidant and Anti-inflammatory Properties of Adansonia digitata and Belanites aegyptiaca Seeds

Journal Of Tropical Pharmacy And Chemistry ◽

10.25026/jtpc.v5i4.296 ◽

2021 ◽

Vol 5 (4) ◽

pp. 286-298

Author(s):

Mercy Badu ◽

Mary-Magdalene Pedavoah ◽

Nathaniel O Boadi ◽

Irene Y Dzaye

Keyword(s):

Antioxidant Capacity ◽

Mineral Elements ◽

Adansonia Digitata ◽

Crude Fibre ◽

Antioxidant Agents ◽

Total Antioxidant ◽

Medicinal Properties ◽

Anti Inflammatory ◽

Mg Content

This study evaluated the nutritional and medicinal properties of seeds from Adansonia digitata (BSF) and Balanite aegyptiaca (DDSF) plant. Proximate chemical composition, mineral elements composition, flavonoids, phenolics, antioxidant capacity, and anti-inflammatory properties were studied. Results obtained revealed that DDSF had the highest moisture, crude fat and crude protein content of 7.66 %, 42.80 %, 20.37 % respectively, whilst BSF gave the highest ash, crude fibre and carbohydrate content. Elemental analysis revealed BSF had the highest Mg content (313.65 mg/100g) and DDSF gave the highest Ca content (118.62 mg/100g). Additionally, DDSF gave the highest total phenolics (18.89 mg TAE/ 100 g), total flavonoids (8.80 mg QE/ 100 g) as well as the highest total antioxidant capacity of (19.62 mg AAE/ 100 g) dry of extract. Based on results obtained in this study, seeds obtained from the Adansonia digitata and Balanite aegyptiaca could be a potential source of functional food and antioxidant agents.

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Anti-inflammatory and Antioxidant Activities of Cucumis sativus Leaves

Bangladesh Pharmaceutical Journal ◽

10.3329/bpj.v18i2.24317 ◽

2015 ◽

Vol 18 (2) ◽

pp. 169-173

Author(s):

Fatema Nasrin ◽

Israt Jahan Bulbul ◽

Fahima Aktar ◽

Mohammad A Rashid

Keyword(s):

Ascorbic Acid ◽

Body Weight ◽

Antioxidant Capacity ◽

Cucumis Sativus ◽

Total Antioxidant Capacity ◽

Antioxidant Activities ◽

Total Phenolic ◽

Paw Edema ◽

Total Antioxidant ◽

Anti Inflammatory

This study was carried out to evaluate the anti-inflammatory and antioxidant activities of Cucumis sativus Linn. (Family: Cucurbitaceae) leaves. The methanolic extract of C. sativus leaves (MCS) was investigated for antiinflammatory activities in Long Evans rat model at two different doses of 150 and 250 mg/kg body weight and the effects were compared with the standard, indomethacin (10 mg/kg body weight). It exhibited highest antiinflammatory activity at the dose 250 mg/kg. In the formalin test, the extract at both doses (150 and 250 mg/kg body weight) significantly prevented the increase in volume of paw edema (P<0.05 and P<0.001). In carrageenan-induced paw edema test the MCS significantly (P?0.001) reduced inflammation by 57.35 % (150 mg/kg body weight) and 72.06% (250 mg/kg body weight) in comparison to the standard drug, indomethacin (79.41%) at the end of 5h. MCS was also screened for DPPH scavenging activity, total antioxidant capacity, reducing ability as well as total phenolics content to assess its antioxidant potential. Total phenolic content and total antioxidant capacity of MCS were found to be 262.31 mg/g equivalent of gallic acid and 267.2 mg/g equivalent of ascorbic acid, respectively. The IC50 of free radical scavenging of DPPH was 13.06 ?g/ml while that of standard ascorbic acid was 13.17 ?g/ml. The reducing power of MCS was found to be concentration dependent.Bangladesh Pharmaceutical Journal 18(2): 169-173, 2015

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Acute moderate-intensity exercise increases total antioxidant capacity and anti-inflammatory responses in competitive cyclists: The role of adiponectin

European Journal of Inflammation ◽

10.1177/2058739221998890 ◽

2021 ◽

Vol 19 ◽

pp. 205873922199889

Author(s):

Tadeja Jakus ◽

Mihaela Jurdana ◽

Lovro Žiberna ◽

Zala J Pražnikar

Keyword(s):

Antioxidant Capacity ◽

Total Antioxidant Capacity ◽

Inflammatory Markers ◽

Inflammatory Responses ◽

The Body ◽

Moderate Intensity ◽

Moderate Intensity Exercise ◽

Total Antioxidant ◽

Competitive Cyclists ◽

Anti Inflammatory

High-intensity exercise can elicit acute changes in the biochemical and physiological processes in the body of an athlete, including increased oxidative stress and inflammation. The purpose of this study was to explore the effect of acute moderate-intensity exercise on total antioxidant capacity (TAC) and serum levels of anti-inflammatory adiponectin (APN), and inflammatory markers in competitive cyclists. Ten male cyclists (age 15–26 years, body mass index 19.4–24.7 kg/m2) participated in this study. Each subject performed the maximal oxygen uptake test (VO2peak) and completed a 10-min cycling exercise at a workload of 50% of the peak of VO2peak. Blood samples were collected on three different occasions: after an overnight fasting and at the exercise workloads of 50% and 100% VO2peak. We measured APN, TAC, inflammatory markers as well as assessed nutrient and energy intake for each participant. Baseline concentration of serum APN (10.92 µg/mL) significantly increased at 50% and at 100% VO2peak. In addition, TAC also increased after acute exercise (0.079 vs 0.093 nmol/µL). The concentration of APN at 50% VO2peak positively correlated with the CRP ( r = 0.640, p = 0.046) and negatively correlated with TNF-α ( r = −0.696, p = 0.025). This test showed that short (10 min) and medium-intensity (50% VO2peak) exercise activity in trained athletes evoked beneficial antioxidant and anti-inflammatory responses. Importantly, this response correlates with the increase in APN levels thereby showing that highly trained individuals have beneficial responses originating from adipose tissue. Our observations show that a short training at moderate activity can be an important preservative strategy during the recovery training period.

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In vitro total antioxidant capacity and anti-inflammatory activity of three common oat-derived avenanthramides

Food Chemistry ◽

10.1016/j.foodchem.2014.03.059 ◽

2014 ◽

Vol 160 ◽

pp. 338-345 ◽

Cited By ~ 42

Author(s):

Jun Yang ◽

Boxin Ou ◽

Mitchell L. Wise ◽

YiFang Chu

Keyword(s):

Antioxidant Capacity ◽

Total Antioxidant Capacity ◽

Inflammatory Activity ◽

Total Antioxidant ◽

Anti Inflammatory

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Investigation on the Effect of Combination of Tanacetum Parthenium and Satureja Montana Methanolic Extracts on the Total Antioxidant Capacity and Serum Nitric Oxide

Journal of Arak University of Medical Sciences ◽

10.32598/jams.24.3.6196.1 ◽

2021 ◽

Vol 24 (3) ◽

pp. 386-397

Author(s):

Zahra Bahramnezhad ◽

◽

Ali Ghazavi ◽

Ali Ganji ◽

Ghasem Mosayebi ◽

...

Keyword(s):

Nitric Oxide ◽

Antioxidant Capacity ◽

Total Antioxidant Capacity ◽

Acute Inflammation ◽

Control Group ◽

Methanolic Extracts ◽

Tanacetum Parthenium ◽

Total Antioxidant ◽

Anti Inflammatory ◽

Satureja Montana

Background and Aim: Medicinal plants contain various biological compounds, including phenols, flavonoids, and terpenoids, with anti-radical and anti-inflammatory activities that affect human health and improve life. Considering the numerous beneficial effects of Tanacetum Parthenium and Satureja Montana and the lack of full antioxidant capacity and anti-inflammatory effects of their combination, we decided to combine these two plants’ anti-inflammatory and antioxidant effects on induced acute inflammation in BALB/c mice. Methods & Materials: In the present study, mice received the extracts of T. Parthenium and S. Montana and their combination by gavage for 14 consecutive days. Then, to induce acute inflammation, thioglycollate was injected intraperitoneally to all groups. FRAP and Grease tests were used to evaluate the total antioxidant capacity and serum nitric oxide concentration, respectively. One-way ANOVA analyzed the results. Ethical Considerations: This study was approved by the Research Ethics Committee of Arak University of Medical Sciences (Code: REC.1398.021). Results: The results showed that the combination of the extracts (P=0.006) and Satureja Montana (P=0.021) led to a significant increase in total antioxidant activity compared to the control group. Also, according to the results of grease test, Satureja montana (P=0.04), Tanacetum parthenium (P=0.034), and their combination (P=0.003) significantly reduced serum nitric oxide production compared to the control group. Conclusion: The present study shows the synergistic effect of the combined extracts to increase their total antioxidant capacity and anti-inflammatory.

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Macronutrients, immunoglobulin A and total antioxidant capacity of human milk during prolonged lactation

10.21203/rs.2.19567/v2 ◽

2020 ◽

Author(s):

krongporn ongprasert ◽

Jetsada Ruangsuriya ◽

Rungnapa Malasao ◽

Ratana Sapbamrer ◽

Pikul Suppansan ◽

...

Keyword(s):

Human Milk ◽

Antioxidant Capacity ◽

Correlation Coefficient ◽

Total Antioxidant Capacity ◽

Energy Content ◽

Pearson Correlation ◽

Immunoglobulin A ◽

Trolox Equivalent Antioxidant Capacity ◽

Healthy Children ◽

Total Antioxidant

Abstract Background: A longer duration of breastfeeding of up to two years is encouraged by many health authorities, but there is limited information regarding the composition of the milk after one year postpartum. The aim of the study was to determine the longitudinal changes in human milk macronutrients, immunoglobulin A (IgA) and total antioxidant capacity (TAC) during prolonged lactation.Methods: One hundred eighty-four breastfeeding mothers with full-term healthy children who had been lactating from 1 to 24 months were recruited from January 2019 to April 2019. Human milk was biochemically analyzed for protein and carbohydrate content by colorimetric assays. The fat content was determined by capillary centrifugation, and the energy content was calculated from the results of centrifugation. IgA levels and TAC were determined by ELISA and a Trolox equivalent antioxidant capacity (TEAC) assay, respectively. Pearson Correlation Coefficient and Spearman’s Rank Correlation Coefficient were used to determine the association of milk composition with month of lactation.Results: The concentrations of fat, energy and IgA were positively correlated with the duration of lactation (r = 0.229, p < 0.01; r = 0.229, p < 0.01 and r = 0.304, p < 0.01, respectively). No significant correlations of protein, carbohydrate concentrations and TAC with the duration of lactation was observed (r = 0.106, p = 0.15; r = -0.032, p = 0.67; r = -0.056, p = 0.45, respectively).Conclusions: We demonstrated that fat, energy and IgA contents increased during prolonged lactation lasting up to two years postpartum, while protein and carbohydrate concentration and TAC were not related to lactation duration. Based on the results, lactating mothers should be encouraged to continue breastfeeding for at least two years postpartum.

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