Denaturation of the SARS-CoV-2 spike protein under non-thermal microwave radiation

SARS-CoV-2, the virus that causes COVID-19, is still a widespread threat to society. The spike protein of this virus facilitates viral entry into the host cell. Here, the denaturation of the S1 subunit of this spike protein by 2.45 GHz electromagnetic radiation was studied quantitatively. The study only pertains to the pure electromagnetic effects by eliminating the bulk heating effect of the microwave radiation in an innovative setup that is capable of controlling the temperature of the sample at any desired intensity of the electromagnetic field. This study was performed at the internal human body temperature, 37 °C, for a relatively short amount of time under a high-power electromagnetic field. The results showed that irradiating the protein with a 700 W, 2.45 GHz electromagnetic field for 2 min can denature the protein to around 95%. In comparison, this is comparable to thermal denaturation at 75 °C for 40 min. Electromagnetic denaturation of the proteins of the virus may open doors to potential therapeutic or sanitation applications.

Variable Molecular Weight Polymer Nanoparticles for Detection and Hyperthermia-Induced Chemotherapy of Colorectal Cancer

Oxaliplatin plays a significant role as a chemotherapeutic agent for the treatment of colorectal cancer (CRC); however, oxaliplatin-resistant phenotypes make further treatment challenging. Here, we have demonstrated that rapid (60 s) hyperthermia (42 °C), generated by the near-infrared stimulation of variable molecular weight nanoparticles (VMWNPs), increases the effectiveness of oxaliplatin in the oxaliplatin-resistant CRC cells. VMWNP-induced hyperthermia resulted in a higher cell death in comparison to cells exposed to chemotherapy at 42 °C for 2 h. Fluorescence from VMWNPs was observed inside cells, which allows for the detection of CRC. The work further demonstrates that the intracellular thermal dose can be determined using cell luminescence and correlated with the cell viability and response to VMWNP-induced chemotherapy. Mild heating makes oxaliplatin-resistant cancer cells responsive to chemotherapy, and the VMWNPs-induced hyperthermia can induce cell death in a few minutes, compared to classical bulk heating. The results presented here lay the foundation for photothermal polymer nanoparticles to be used for cell ablation and augmenting chemotherapy in drug-resistant colorectal cancer cells.

Inductor for Uniform Bulk Heat Treatment of Welded Butt Joints of Railway Rails

To improve the structure of metal in welded butt joints of railway rails produced by flash-butt welding and increase the reliability of butt joints, it is advisable to carry out their induction heat treatment using high-frequency currents. Solving the problem of a uniform bulk heating of weld metal of railway rails in a narrow area during its heat treatment remains an urgent task. The work describes the principle of designing an inductor without magnetic cores for carrying out a uniform bulk heat treatment of welded butt joints of railway rails for realization of favorable phase transformations of metal and normalization of its structure. The principle is based on the physical laws of propagation of electromagnetic fields and electric currents in the inductor and a rail. Based on the carried out investigations, an inductor was designed that has a variable shape along the perimeter of a rail and a variable distance from it, as well as a partial splitting of the inductor busbar for current parallelization, which provides a uniform bulk heating of a rail butt joint. Splitting of the inductor busbar allowed adjusting the propagation of currents in the inductor and a rail in such a way as to avoid overheating of a rail in its particular areas without a significant increase in the distance between the inductor and a rail, and respectively without a significant increase in the reactive power of the “inductor-product” system. The carried out experiments on heating the welded butt joint of a rail by the designed inductor showed the indices of uniformity and rate of its bulk heating, which are acceptable for heat treatment of rails both on the surface as well as in the depth of a rail in a narrow heating zone with providing the required temperature levels.

Fast collisional electron heating and relaxation in thin foils driven by a circularly polarized ultraintense short-pulse laser

The creation of well-thermalized, hot and dense plasmas is attractive for warm dense matter studies. We investigate collisionally induced energy absorption of an ultraintense and ultrashort laser pulse in a solid copper target using particle-in-cell simulations. We find that, upon irradiation by a $2\times 10^{20}~\text{W}\,\text{cm}^{-2}$ intensity, 60 fs duration, circularly polarized laser pulse, the electrons in the collisional simulation rapidly reach a well-thermalized distribution with ${\sim}3.5~\text{keV}$ temperature, while in the collisionless simulation the absorption is several orders of magnitude weaker. Circular polarization inhibits the generation of suprathermal electrons, while ensuring efficient bulk heating through inverse bremsstrahlung, a mechanism usually overlooked at relativistic laser intensity. An additional simulation, taking account of both collisional and field ionization, yields similar results: the bulk electrons are heated to ${\sim}2.5~\text{keV}$ , but with a somewhat lower degree of thermalization than in the pre-set, fixed-ionization case. The collisional absorption mechanism is found to be robust against variations in the laser parameters. At fixed laser pulse energy, increasing the pulse duration rather than the intensity leads to a higher electron temperature.

Numerical Characterization of Viscous Heat Dissipation Rate in Oscillatory Air Flow

The effect of viscous heat dissipation (VHD) in raising the temperature field of incompressible oscillatory air flow is studied numerically. A threshold is established for when the viscous heat dissipation term in the thermal energy equation changes or does not change the temperature field for the case of oscillatory air flow in a tube connecting two reservoirs. This new criterion has not been specified clearly in earlier oscillatory flow research. According to the defined threshold and when VHD is important, the effect of dissipative bulk heating can be described by a proposed correlation in terms of Womersley number (Wo) and axial tidal displacement (ΔZ) of the oscillatory fluid. These results are determined using two-dimensional (2D) numerical simulations of laminar oscillatory air flow (Pr = 0.7) for different adiabatic unconductive tube-reservoirs' systems configurations over a wide range of oscillatory frequencies and tidal displacements. It is found that the low amount of fluid kinetic energy, which is converted into internal energy, is not sufficient to significantly heat up the fluid at a low rate of the viscous work. Therefore, the effect of viscous heat dissipation in oscillatory air flow can be ignored only below a specific limit of unsteadiness depending on Womersley number and axial tidal displacement. Also, the results showed that the VHD becomes more significant with increasing (Wo) and (ΔZ).

Investigation of Heterogeneous Joule Heating as the Explanation for the Transient Electroplastic Stress Drop in Pulsed Tension of 7075-T6 Aluminum

The electroplastic effect can be predicted and modeled as a 100% bulk heating/softening phenomenon in the quasi-steady-state; however, these same models do not accurately predict flow stress in transient cases. In this work, heterogeneous Joule heating is examined as the possible cause for the transient stress drop during quasi-static pulsed tension of 7075-T6 aluminum. A multiscale finite element model is constructed where heterogeneous thermal softening is explored through the representation of grains, grain boundaries, and precipitates. Electrical resistivity is modeled as a function of temperature and dislocation density. In order to drive the model to predict the observed stress drop, the bulk temperature of the specimen exceeds experiment, while the dislocation density and grain boundary electrical resistivity exceed published values, thereby suggesting that microscale heterogeneous heating theory is not the full explanation for the transient electroplastic effect. A new theory for explaining the electroplastic effect based on dissolution of bonds is proposed called the Electron Stagnation Theory.