scholarly journals Recurring exposure to low humidity induces transcriptional and protein level changes in the vocal folds of rabbits

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Taylor W. Bailey ◽  
Andrea Pires dos Santos ◽  
Naila Cannes do Nascimento ◽  
Jun Xie ◽  
M. Preeti Sivasankar ◽  
...  
Keyword(s):  
Stress Response ◽  
Voice Disorders ◽  
Preventive Measure ◽  
Vocal Folds ◽  
Health Condition ◽  
Cellular Level ◽  
Tissue Samples ◽  
Environment Exposure ◽  
Low Humidity ◽  
Humidity Environment

AbstractVoice disorders are an important human health condition. Hydration is a commonly recommended preventive measure for voice disorders though it is unclear how vocal fold dehydration is harmful at the cellular level. Airway surface dehydration can result from exposure to low humidity air. Here we have induced airway surface dehydration in New Zealand White rabbits exposed to a recurring 8-h low humidity environment over 15 days. This model mimics an occupational exposure to a low humidity environment. Exposure to moderate humidity was the control condition. Full thickness soft-tissue samples, including the vocal folds and surrounding laryngeal tissue, were collected for molecular analysis. RT-qPCR demonstrated a significant upregulation of MUC4 (mucin 4) and SCL26A9 (chloride channel) and a large fold-change though statistically non-significant upregulation of SCNNA1 (epithelial sodium channel). Proteomic analysis demonstrated differential regulation of proteins clustering into prospective functional groups of muscle structure and function, oxidative stress response, and protein chaperonin stress response. Together, the data demonstrate that recurring exposure to low humidity is sufficient to induce both transcriptional and translational level changes in laryngeal tissue and suggest that low humidity exposure induces cellular stress at the level of the vocal folds.

Biomedical applications: Pitfalls in the practice

1994 ◽  
Vol 52 ◽  
pp. 378-379
Author(s):  
J. D. Shelburne ◽  
Peter Ingram ◽  
Victor L. Roggli ◽  
Ann LeFurgey
Keyword(s):  
Operating Room ◽  
Liquid Nitrogen ◽  
Lung Tissue ◽  
Biomedical Applications ◽  
Microprobe Analysis ◽  
Tissue Sample ◽  
Cellular Level ◽  
Tissue Samples ◽  
Asbestos Fibers

At present most medical microprobe analysis is conducted on insoluble particulates such as asbestos fibers in lung tissue. Cryotechniques are not necessary for this type of specimen. Insoluble particulates can be processed conventionally. Nevertheless, it is important to emphasize that conventional processing is unacceptable for specimens in which electrolyte distributions in tissues are sought. It is necessary to flash-freeze in order to preserve the integrity of electrolyte distributions at the subcellular and cellular level. Ideally, biopsies should be flash-frozen in the operating room rather than being frozen several minutes later in a histology laboratory. Electrolytes will move during such a long delay. While flammable cryogens such as propane obviously cannot be used in an operating room, liquid nitrogen-cooled slam-freezing devices or guns may be permitted, and are the best way to achieve an artifact-free, accurate tissue sample which truly reflects the in vivo state. Unfortunately, the importance of cryofixation is often not understood. Investigators bring tissue samples fixed in glutaraldehyde to a microprobe laboratory with a request for microprobe analysis for electrolytes.

Prospects for the use of omix-technologies in preclinical practice of production-related diseases of the voice

2020 ◽  
pp. 541-541
Author(s):  
Y. S. Alizade ◽  
L. B. Rudin
Keyword(s):  
At Risk ◽  
Minimally Invasive ◽  
Voice Disorders ◽  
Vocal Folds ◽  
The Voice

The potential predictive possibilities of minimally invasive prenosological diagnosis of voice disorders on the basis of combined Geno - and phenotyping of persons at risk of diseases of the vocal folds of professional origin.

Videostrobolaryngoscopy of Mucus Layer during Vocal Fold Vibration in Patients with Laryngeal Tension-Fatigue Syndrome

2002 ◽  
Vol 111 (6) ◽  
pp. 537-541 ◽  
Author(s):  
Tzu-Yu Hsiao ◽  
Chia-Ming Liu ◽  
Kai-Nan Lin
Keyword(s):  
Mechanical Properties ◽  
Vocal Fold ◽  
Rough Surfaces ◽  
Voice Disorders ◽  
Vocal Folds ◽  
Voice Disorder ◽  
Mucus Layer ◽  
Vocal Fold Vibration ◽  
Fatigue Syndrome ◽  
Functional Dysphonia

The mucus layer on the vocal folds was examined by videostrobolaryngoscopy in patients with laryngeal tension-fatigue syndrome, a chronic functional dysphonia due to vocal abuse and misuse. Besides the findings in previous reports (such as abnormal glottal closure, phase or amplitude asymmetry, and the irregular mucosal wave), the vocal folds during vibration had an uneven mucus surface. The occurrence of an uneven mucus layer on vocal folds was significantly greater in subjects with this voice disorder (83% or 250 of 301 patients in this series) than in those without voice disorders (18.5% or 5 of 27). The increase of mucus viscosity, mucus aggregation, and the formation of rough surfaces on the vocal folds alter the mechanical properties that contribute to vibration of the cover of the vocal folds, and thereby worsen the symptoms of dysphonia in patients with laryngeal tension-fatigue syndrome.

scholarly journals The effect of curing conditions on the expansion efficiency of MgO expansion agent

2021 ◽  
Vol 233 ◽  
pp. 03047
Author(s):  
Yu Fang ◽  
Li Yongchao
Keyword(s):  
Curing Temperature ◽  
Obvious Effect ◽  
Curing Conditions ◽  
Factors Affecting ◽  
Temperature Conditions ◽  
Low Humidity ◽  
Humidity Environment

In order to further promote the research and application of MgO expansion agent in concrete field, this paper carried out the effect of different humidity and temperature conditions on the expansion properties of mortar and mortar specimens mixed with MgO expansion agent. In addition, the mechanism of the factors affecting the sensitivity of the MgO expansion agent is revealed by combining microscopic technology. The results show that the higher the curing temperature and the greater the curing humidity, the greater the expansion efficiency of the MgO expansion agent. The temperature of 20~40°C has no obvious effect on the efficiency of the MgO expansion agent, but the expansion value of the specimen doubles as the temperature rises to 40~80°C. Besides, the higher the curing humidity, the better the expansion efficiency of the MgO expansion agent, but the MgO expansion agent is more sensitive to the low humidity environment, and the specimen shrinks in the lower humidity environment (RH=60%).

scholarly journals SPECIFICS AND DIAGNOSTIC PROCEDURE IN REFLUX-RELATED DYSPHONIA

2020 ◽  
Vol 73 (4) ◽  
pp. 814-817
Author(s):  
Klaudia Sowa ◽  
Anna Łobaczuk-Sitnik ◽  
Bartosz Piszczatowski ◽  
Emilia Duchnowska ◽  
Bożena Kosztyła-Hojna ◽  
...  
Keyword(s):  
Chronic Diseases ◽  
Reflux Disease ◽  
Laryngopharyngeal Reflux ◽  
Morphological Changes ◽  
Voice Disorders ◽  
Vocal Folds ◽  
Congenital Defects ◽  
Upper Respiratory Tract ◽  
Laryngopharyngeal Reflux Disease ◽  
Pharynx Cancer

Voice is a work tool for many professional groups. Currently, cases of dysphonia of multiple origin consist a growing issue. Voice disorders may result from disturbed voice production process, congenital defects, post-traumatic conditions, chronic diseases or hormonal disorders. Chronic diseases causing voice disorders include laryngopharyngeal reflux disease and esophageal reflux disease. The chronic character of reflux causes the formation of numerous morphological changes of the larynx, including: hyperemia of the mucosa limited to arytenoid and intraarytenoid area, edema of the vocal folds, edema of the larynx mucosa. These changes contribute to voice disorders. Among the pathological changes of voice organ etiologically associated with reflux, the following disease units may be distinguished: reflux laryngitis, subglottic edema, contact ulceration, larynx granuloma, larynx and pharynx cancer. Many of disorders in the upper respiratory tract are etiologically related to reflux, e.g. dysphonia, grunting, coughing and dyspnoea.

scholarly journals A Deep Learning Enhanced Novel Software Tool for Laryngeal Dynamics Analysis

2021 ◽  
pp. 1-15
Author(s):  
Andreas M. Kist ◽  
Pablo Gómez ◽  
Denis Dubrovskiy ◽  
Patrick Schlegel ◽  
Melda Kunduk ◽  
...  
Keyword(s):  
Neural Networks ◽  
Quantitative Analysis ◽  
High Speed ◽  
Voice Disorders ◽  
Vocal Folds ◽  
Video Data ◽  
Audio Data ◽  
Fully Automatic ◽  
Video And Audio

Purpose High-speed videoendoscopy (HSV) is an emerging, but barely used, endoscopy technique in the clinic to assess and diagnose voice disorders because of the lack of dedicated software to analyze the data. HSV allows to quantify the vocal fold oscillations by segmenting the glottal area. This challenging task has been tackled by various studies; however, the proposed approaches are mostly limited and not suitable for daily clinical routine. Method We developed a user-friendly software in C# that allows the editing, motion correction, segmentation, and quantitative analysis of HSV data. We further provide pretrained deep neural networks for fully automatic glottis segmentation. Results We freely provide our software Glottis Analysis Tools (GAT). Using GAT, we provide a general threshold-based region growing platform that enables the user to analyze data from various sources, such as in vivo recordings, ex vivo recordings, and high-speed footage of artificial vocal folds. Additionally, especially for in vivo recordings, we provide three robust neural networks at various speed and quality settings to allow a fully automatic glottis segmentation needed for application by untrained personnel. GAT further evaluates video and audio data in parallel and is able to extract various features from the video data, among others the glottal area waveform, that is, the changing glottal area over time. In total, GAT provides 79 unique quantitative analysis parameters for video- and audio-based signals. Many of these parameters have already been shown to reflect voice disorders, highlighting the clinical importance and usefulness of the GAT software. Conclusion GAT is a unique tool to process HSV and audio data to determine quantitative, clinically relevant parameters for research, diagnosis, and treatment of laryngeal disorders. Supplemental Material https://doi.org/10.23641/asha.14575533

Presbylarynx, Functional Voice Disorders, Muscle Tension Dysphonia

2021 ◽  
Author(s):  
Elizabeth Erickson-DiRenzo ◽  
Christine M. Kim ◽  
C. Kwang Sung Sung
Keyword(s):  
Muscle Tension ◽  
Voice Disorders ◽  
Neuromuscular Control ◽  
Vocal Folds ◽  
Voice Therapy ◽  
Type I ◽  
Muscle Tension Dysphonia ◽  
Functional Dysphonia ◽  
Age Related ◽  
Type I Thyroplasty

Presbylarynx refers to age-related structural changes of the vocal folds that include muscle atrophy, reduced neuromuscular control, loss of superficial lamina propria layer, and reduced pliability. The changes result in thin and bowed vocal folds, increased vocal effort requirements, breathy voice, change in habitual pitch, and strain. The primary treatment options are voice therapy focused on strengthening breath support and the intrinsic muscles of the larynx, and optimization of resonance; injection augmentation of the vocal folds; and type I thyroplasty. Functional dysphonia is defined as change in voice quality in the absence of structural or neurological abnormalities of the larynx. Muscle tension dysphonia (MTD) is a subtype of functional voice disorders and involves laryngeal muscle tension imbalance due to excessive or dysregulated activation resulting often in strained or breathy voice. MTD can be divided into primary (psychological etiology or vocal misuse) and secondary (compensatory for organic laryngeal pathology). The mainstay of treatment for MTD is voice therapy, along with medical or surgical treatment of the underlying vocal pathology in secondary MTD. Mutational falsetto, or puberphonia, is a functional voice disorder where a high-pitched, pre-adolescent voice fails to transition to the lower pitch of adulthood. This review contains 5 figures, 7 tables, 4 videos and 10 references Key Words: Presbylarynx, Injection augmentation, Type I thyroplasty, Primary muscle tension dysphonia, Secondary muscle tension dysphonia, Muscle tension patterns, Manual circumlaryngeal therapy, Functional dysphonia, Mutational falsetto  

PERFORMANCE ASSESSMENT OF A SOLID DESICCANT AIR DEHUMIDIFIER

2016 ◽  
Vol 78 (8-4) ◽  
Author(s):  
Nazri Kamsah ◽  
Haslinda Mohamed Kamar ◽  
Muhammad Imran Wan Khairuzzaman ◽  
M. Idrus Alhamid ◽  
Fazila Mohd Zawawi
Keyword(s):  
Removal Rate ◽  
Operating Parameters ◽  
Inlet Temperature ◽  
Moisture Removal ◽  
Desiccant Wheel ◽  
Air Inlet ◽  
Low Humidity ◽  
Experimental Rig ◽  
Humidity Environment

The presence of moisture in the air along with temperature has a long term and devastating effect on man and material. One way to create a low humidity environment is by using a solid desiccant wheel system. In the present work, an experimental analysis has been carried out under steady-state conditions to investigate the effects of different operating parameters on a solid desiccant wheel system performances. An experimental rig consists of an FFB300 air dehumidifier system was constructed. A parametric investigation was carried out to examine the effects of the reactivation air inlet temperature and process air outlet velocity on the thermal effectiveness, dehumidification efficiency, and moisture removal rate of the desiccant wheel system. The analysis shows that both thermal effectiveness and dehumidification efficiency decrease with the increase of the reactivation air inlet temperature, by 2.5 % and 43 %, respectively. Likewise, when the process air outlet velocity increases both performances criteria reduce by 10 % and 28 %, respectively. The moisture removal rate increases significantly by 30 % as the reactivation air inlet temperature increases. However, the process air outlet velocity has no significant effect on the moisture removal rate. 

scholarly journals Unidirectional Regulation of Vimentin Intermediate Filaments to Caveolin-1

2020 ◽  
Vol 21 (20) ◽  
pp. 7436
Author(s):  
Xuemeng Shi ◽  
Changyuan Fan ◽  
Yaming Jiu
Keyword(s):  
Cell Migration ◽  
Stress Response ◽  
Intermediate Filaments ◽  
Migration Rate ◽  
Osmotic Shock ◽  
Cellular Level ◽  
Caveolin 1 ◽  
Active Processes ◽  
Intracellular Motility ◽  
Vimentin Filaments

Both the mechanosensitive vimentin cytoskeleton and endocytic caveolae contribute to various active processes such as cell migration, morphogenesis, and stress response. However, the crosstalk between these two systems has remained elusive. Here, we find that the subcellular expression between vimentin and caveolin-1 is mutual exclusive, and vimentin filaments physically arrest the cytoplasmic motility of caveolin-1 vesicles. Importantly, vimentin depletion increases the phosphorylation of caveolin-1 on site Tyr14, and restores the compromised cell migration rate and directionality caused by caveolin-1 deprivation. Moreover, upon hypo-osmotic shock, vimentin-knockout recovers the reduced intracellular motility of caveolin-1 vesicles. In contrary, caveolin-1 depletion shows no effect on the expression, phosphorylation (on sites Ser39, Ser56, and Ser83), distribution, solubility, and cellular dynamics of vimentin filaments. Taken together, our data reveals a unidirectional regulation of vimentin to caveolin-1, at least on the cellular level.

Objectification of vocal folds mucosal wave

2018 ◽  
Vol 72 (5) ◽  
pp. 24-30
Author(s):  
Paulina Krasnodębska ◽  
Wiktor Krasnodębski ◽  
Agata Szkiełkowska
Keyword(s):  
Clinical Diagnosis ◽  
Sensitivity And Specificity ◽  
Clinical Utility ◽  
Voice Disorders ◽  
High Sensitivity ◽  
Vocal Folds ◽  
Wave Measurement ◽  
Voice Pathologies

The work assessed the clinical utility of open quotients measured during laryngovideostroboscopy and electroglottography. Values of the parameters were analyzed according to clinical diagnosis. An algorithm based on direct and indirect mucosal wave measurement is presented, which allows for differentiation of voice disorders. The method developed for the objectification of glottal functions in various voice pathologies is characterized by high sensitivity and specificity.

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