First DNA Barcoding Based Record of Lysiosquillina Maculata (Fabricius, 1793) (Crustacea: Stomatopoda) From Chennai Coast, Tamil Nadu, India

Taxonomic identification of mantis shrimp Lysiosquillina maculata through DNA barcoding analysis collected from Kasimedu fisheries harbour, Chennai coast, Tamil Nadu, India. The mitochondrial cytochrome oxidase sub unit I gene (mtcoI) with 650 bp region was sequenced for phylogenetic analysis. The present record, mitochondrial gene sequences were used to identify the mantis shrimp Lysiosquillina maculata. This is the first confirmed record of Indian waters and the mt COI sequence was deposited in GenBank. The neighbor joining method was used for phylogenetic analysis. The pair wise genetic distance calculated with 08 closely related species varied form 0.03-0.404%. Phylogenetic tree based on 13 protein coding genes shows that Lysiosquillina maculata has a closer phylogenetic relationship to Harpiosquilla harpax.

Köyceğiz-Dalyan Lagün Ekosistemi Kefal Populasyonlarının Büyüme Performans İndeksleri

As it is common in tropical and temperate zones, mullet species constitute the most important fish species caught along the coast and estuaries of Turkey. In terms of ecology, tourism and and fish productivity (52 kg/ha), Koycegiz-Dalyan Lagoon Ecosystem (KDLES) is the most important lagoon, with a 5500 ha surface area, is the Mediterranean Coast of Turkey. In the dalyan (barrier fish trap) systems; approximately 225 tons (80-405 tons) of mullets are caught to obtain 5 tons of dried fish eggs per year. Seventy percent of fish production in the lagoon is composed of mullets. The mullet samples were collected monthly between October 1992 and September 1994 from eight stations (one of them is a marine station) by trammel nets. The ages were determined using by scales anda ge composition varied form 0 to 6 ages. Species composition of 1159 sampled individuals were proportionally determined as Mugil cephalus 45%, Liza aurata 35%, Liza saliens 14%, Liza ramada 12%, Chelon labrasus 6%. The growth performance indices calculated from von Bertalanffy growth parameters using by all individuals for the M. cephalus, L. aurata, L. saliens, L. ramada and C. labrasus were determined as 2.957, 2.866, 2.692 and 2.719, respectively. The highest growth performance index was calculated for M. cephalus. The growth performance indeces of mullet species in Köyceğiz-Dalyan Lagoon Ecosystem were found around the world averages.

Superior Mesenteric Artery Thrombosis for Emergency Laparotomy in Covid-19 Patient: A Case Report

The novel coronavirus disease 2019 (COVID-19) pandemic has caused significant morbidity and mortality worldwide. The most common symptoms include fever, cough, and shortness of breath (predominantly involving pulmonary function). However, there are lesser-known presentations that affect other systems too. Vascular complications have been reported in the literature focusing on venous thrombosis, which appears to be more common than arterial thrombosis. Among arterial thrombosis, mainly lower limb involvement is reported so far. Here, we report an unusual presentation in COVID-19, which presented with acute abdomen due to superior mesenteric artery thrombosis without respiratory symptoms. We report this case as a varied form of coronavirus complication presentation and highlight the need for eternal vigilance and a reactive approach for early diagnosis.

Differential Glycosite Profiling—A Versatile Method to Compare Membrane Glycoproteomes

Glycosylation is the most prevalent and varied form of post-translational protein modifications. Protein glycosylation regulates multiple cellular functions, including protein folding, cell adhesion, molecular trafficking and clearance, receptor activation, signal transduction, and endocytosis. In particular, membrane proteins are frequently highly glycosylated, which is both linked to physiological processes and of high relevance in various disease mechanisms. The cellular glycome is increasingly considered to be a therapeutic target. Here we describe a new strategy to compare membrane glycoproteomes, thereby identifying proteins with altered glycan structures and the respective glycosites. The workflow started with an optimized procedure for the digestion of membrane proteins followed by the lectin-based isolation of glycopeptides. Since alterations in the glycan part of a glycopeptide cause mass alterations, analytical size exclusion chromatography was applied to detect these mass shifts. N-glycosidase treatment combined with nanoUPLC-coupled mass spectrometry identified the altered glycoproteins and respective glycosites. The methodology was established using the colon cancer cell line CX1, which was treated with 2-deoxy-glucose—a modulator of N-glycosylation. The described methodology is not restricted to cell culture, as it can also be adapted to tissue samples or body fluids. Altogether, it is a useful module in various experimental settings that target glycan functions.

Etoricoxib and Herbal Adjuvant Consisting Nanoparticles Incorporated Gel and Ointment Formulations for the Treatment of Human Skin Melanoma (SK-MEL-2)

Aim: The aim of study was to develop nanoparticles consisting etoricoxib with ethanolic extract of ginger rhizome (GE) and basil leaves (BE) and evaluated against human skin cancer cells (melanoma, SK-MEL-2). Nanoparticles were further incorporated into gel and ointment and characterized. Material: Concentration of extract was varied while etoricoxib remains constant in nanoparticles. Nanoparticles were incorporated into gel and ointment, prepared by using tamarind seed polysaccharide and aloe vera gel, respectively. Result: All the batches of nanoparticles were evaluated for particle size and were found from 924 nm (N1) to 1084 nm (N9). The loading efficiency of etoricoxib varied form 66.8±0.05 % (N4) to 85.1±0.04 % (N9), for GE 60.3±0.04 % (N4) to 72.1±0.05 % (N9), for BE 59.5±0.04 % (N8) to 80.5±0.03 % (N9). The consistency of ointment and gel was found smooth and no greediness observed. The pH of the nanoparticles incorporated ointment was observed 6.2±0.023 (O6) to 6.2±0.089 (O4), viscosity was found from 0.70±0.098 (O3) to 1.130±0.092 (O1) gm/cm3 , spreadability in the range of 58.3±0.062 (O2) to 66.2±0.098 (O5) %. The pH of nanoparticles incorporated gel was observed 6.2±0.019 (G2) to 6.2±0.098 (G7), viscosity was found from 0.847±0.030 (G7) to 1.130±0.065 gm/cm3 (G9), spreadability in the range of 62.5±0.045 % (G5) to 70.51±0.056 % (G4). In vitro cytotoxic studies showed that nanoparticles incorporated gel formulation (G1) was able to control cell grown (SK-MEL-2). Conclusion: It can be concluded that etoricoxib and herbal components consisting formulations were able to control human skin cancer cells.

Estimates of the Population Parameters and Exploitation Rate of Pontic Shad (Alosa Immaculata Bennett, 1835) in the Romanian Black Sea Coast

Sex ratio, morphometric characteristics, age and growth for Pontic shad from Romanian Black Sea Coast were examined. A total of 2.133 individuals were caught between March 2012 and September 2013. Female : male sex ratio was 0.62. According to the age reading, distribution varied form II to V year. The von Bertalanffy equation and growth performance index were determined by ESP software in Pontic shad. The asymptotic length (L∞ = L infinity) of Pontic shad generated by the ELEFAN I method, after introducing total length data for the two years of study, was 41.5. The natural mortality instantaneous coefficient (M) of the entire Pontic shad population, calculated according to Pauly’s empirical equation, using the growth parameters of the Von Bertalanffy formula and the mean annual temperature of the two study years of 13°C, recorded the following values: M = 0.585 in 2012 and M = 0.639 in 2013. According with the resultants, it is recommended to perform a sustainable fishing which allows the capture of legal-sized of fish.

Management Praxis at Nepalese Manufacturing and Non-Manufacturing Firms

This paper ascertains management praxis at Nepalese manufacturing and non-manufacturing firms that are shaping the firms’ decisions and actions in due course of their managerial process activities. In-depth interviews with selected executives from among the respondents and discourse analysis thereof indicate a varied form of managerial practices. The discourse in the areas of organizational change and management praxis concentrated on the adaptation to their environment. It focuses on the role of executives, culture, environmental change, organizational and business legacy in consideration of discontinuity, innovation and social adaptation. Chisquare test of independence and Kendall’s tau-b have been instrumental in interpretation of the associations and relationships of the principal actors of change makers in organizations and businesses. Pravaha Vol. 24, No. 1, 2018, Page: 159-174

PERUBAHAN KATA “TIADA” DALAM TIGA KARYA SASTRA: BUSTAN AS-SALATIN (1640), HIKAYAT SIAK (1855), DAN KETIKA CINTA BERTASBIH 2 (2009)

<p><em>In the reality, language will always change. One of examples is the change of language that used in Malay literature manuscripts. In this research, researcher analyzed the language change of “</em>tiada”<em> word that occured in three different periods of manuscript. The approaches used in this research were semantic, morphologic, and syntactic approach. In analyzing those approaches, researcher used semantic restrictions from Verhaar (1992), morphologic restriction from Chaer (1994), and syntactic restriction from Kridalaksana (1999). The researcher used descriptive analysis method. The data used in this research were three literary manuscripts with a span of two centuries, Bustan as-Salatin (1640), Hikayat Siak (1855), and Ketika Cinta Bertasbih 2 (2009). The analysis of this research resulted information that containing “</em>tiada”<em> which covered all of the language aspects; semantic, morphologic, and syntactic. From the analysis, the researcher got the conclusions that </em>“tiada”<em> in the manuscript at the beginning period had more varied form than the manuscript in the two periods thereafter. Then, in the final period, there were changes of </em>“tiada” <em>that undergoes semantic and morphologic changes from the text of the two previous periods.</em><br /><br />Dalam kenyataannya, bahasa akan selalu berubah. Salah satu contohnya adalah perubahan bahasa yang digunakan dalam naskah sastra Melayu. Dalam penelitian ini, peneliti menganalisis perubahan bahasa kata "tiada" yang terjadi dalam tiga periode naskah yang berbeda. Pendekatan yang digunakan dalam penelitian ini adalah pendekatan semantik, morfologi, dan sintaksis. Dalam menganalisis pendekatan tersebut, peneliti menggunakan batasan semantik dari Verhaar (1992), pembatasan morfologis dari Chaer (1994), dan pembatasan sintaksis dari Kridalaksana (1999). Peneliti menggunakan metode analisis deskriptif. Data yang digunakan dalam penelitian ini adalah tiga naskah sastra dengan rentang waktu dua abad, Bustan as-Salatin (1640), Hikayat Siak (1855), dan Ketika Cinta Bertasbih 2 (2009). Analisis penelitian ini menghasilkan informasi yang mengandung "tiada" yang mencakup semua aspek bahasa; semantik, morfologis, dan sintaksis. Dari hasil analisis, peneliti mendapatkan kesimpulan bahwa "tiada" dalam naskah pada periode awal memiliki bentuk yang lebih bervariasi daripada manuskrip dalam dua periode sesudahnya. Kemudian, pada periode akhir, terjadi perubahan "tiada" yang mengalami perubahan semantik dan morfologis dari teks dua periode sebelumnya.</p>

EVALUASI BEBERAPA JENIS PRODUK INOKULAN GAHARU DI KALIMANTAN SELATAN

The availability of natural agarwood tends to decrease and encourage some parties to cultivate agarwood producing plants by using biological inoculation method that is microbe. In relation to this, many manufacturers produce inoculant products used to inoculate agarwood trees. This research is aimed to get some agarwood inoculant products in South Kalimantan. The inoculant products used in this study were obtained based on information from agarwood farmers in South Kalimantan. Based on observation result, inokulan products have varied form ie liquid, capsule, and stick. In addition, these products do not fullfil the packaging standards such as the absence of date of manufacture, trade license, and expiry date. The result of identification of dominant microbes inokulan product design showed some products formed from microbial species that function in agarwood formation that have not been studied even at risk of endangering human health. The results of this study are expected to be an input for farmers to be more careful in choosing the right inoculantproducts and become a driver for producers to improve the quality of inoculants produced.slsKeyword: inoculants; fungi; agarwood; quality; south kalimantanKetersediaan gubal gaharu alam semakin menurun, mendorong beberapa pihak untuk membudidayakan tanaman penghasil gaharu dengan menggunakan metode inokulasi biologis yaitu mikroba. Sehubungan dengan hal tersebut, banyak produsen membuat produk inokulan yang digunakan untuk menginokulasi pohon gaharu. Penelitian ini bertujuan untuk mendapatkan beberapa produk inokulan gaharu yang ada di Kalimantan Selatan. Produk-produk inokulan yang digunakan pada studi ini diperoleh berdasarkan informasi petani gaharu di Kalimantan Selatan. Berdasarkan hasil pengamatan diketahui produk-produk inokulan memiliki bentuk bervariasi yakni cair, kapsul, dan stik. Selain itu, produk-produk tersebut belum memenuhi standar kemasan antara lain tidak adanya informasi tanggal pembuatan, ijn dagang, dan tanggal kadaluarsa. Hasil identifikasi mikroba dominan penyusun produk inokulan menunjukkan beberapa produk terbentuk dari jenis-jenis mikroba yang fungsinya dalam pembentukan gaharu belum banyak dipelajari bahkan beresiko membahayakan kesehatan manusia. Hasil penelitian ini diharapkan dapat menjadi masukan bagi petani untuk lebih teliti dalam memilih produk inokulan yang tepat serta menjadi pendorong bagi produsen untuk meningkatkan mutu inokulan yang diproduksi.Kata kunci : inokulan; cendawan; gaharu; kualitas; Kalimantan Selatan

Examination of the interval between litters (IBL) of different genotype HLW sows using survival analysis

In this study our aim was to find out if there is a difference between the genotypes determined for the previously identified mutations of seven genes of the Hungarian Large White in terms of the time spent in production. We identified the previously determined alleles of the seven genes (BF, EGF, ESR, FSHβ, H2AFZ, LEP, PRLR) related to proliferation that were and performed the survival analysis between breeds indicating the risk of culling and the time spent in production on the given farm. Based on the results of survival analysis by Log-rank test, Breslow (Generalized Wilcoxon) and Tarone-Ware test we concluded that they indicated a significant difference in case of the genes BF (Breslow and Tarone-Ware tests) the EGF (Log-rank and Tarone-Ware tests) and ESR (Log rank test) based on which the curves of the survival of the certain genes varied form one another significantly.