Study on the temperament of cows of the Aberdeen Angus cattle breed

The present study examined the temperament of 699 cows of the Aberdeen Angus cattle breed, reared in 14 farms in Bulgaria. It was carried out within the period 2017–2020. The animals subject to the study were between two and eight years old. The temperament was visually evaluated following a scoring system from 1 to 5. The assessment method involved the behavioural reaction of the cows upon manipulation, passing through a chute and fixation into a cattle crush. The temperament of cows reared in two different systems – intensive and semi-intensive farming was compared. The temperament of the Aberdeen Angus cows had an average rate of 2.74 ± 0.04. The cows which had frequent contact with people had a calmer temperament. The manner of rearing (P < 0.001) as well as the age group (P < 0.001) had a significant influence on the parameter examined. The young animals were calmer and got accustomed to working in a crush more easily when they were reared together with cows of different ages.

Sociability strongly affects the behavioural responses of wild guanacos to drones

Drones are being increasingly used in research and recreation but without an adequate assessment of their potential impacts on wildlife. Particularly, the effect of sociability on behavioural responses to drone-associated disturbance remains largely unknown. Using an ungulate with complex social behaviour, we (1) assessed how social aggregation and offspring presence, along with flight plan characteristics, influence the probability of behavioural reaction and the flight distance of wild guanacos (Lama guanicoe) to the drone's approach, and (2) estimated reaction thresholds and flight heights that minimise disturbance. Sociability significantly affected behavioural responses. Large groups showed higher reaction probability and greater flight distances than smaller groups and solitary individuals, regardless of the presence of offspring. This suggests greater detection abilities in large groups, but we cannot rule out the influence of other features inherent to each social unit (e.g., territoriality) that might be working simultaneously. Low flight heights increased the probability of reaction, although the effect of drone speed was less clear. Reaction thresholds ranged from 154 m (solitary individuals) to 344 m (mixed groups), revealing that the responsiveness of this guanaco population to the drone is the most dramatic reported so far for a wild species.

The regulatory role of GABAA receptor in Actinia equina nervous system and the possible effect of global ocean acidification

Global warming and connected acidification of the world ocean attract a substantial amount of research efforts, in particular in a context of their impact on behaviour and metabolism of marine organisms, such as Cnidaria. Nevertheless, mechanisms underlying Cnidarians’ neural signalling and behaviour and their (possible) alterations due to the world ocean acidification remain poorly understood. Here we researched for the first time modulation of GABAA receptors (GABAARs) in Actinia equina (Cnidaria: Anthozoa) by pH fluctuations within a range predicted by the world ocean acidification scenarios for the next 80–100 years and by selective pharmacological activation. We found that in line with earlier studies on vertebrates, both changes of pH and activation of GABAARs with a selective allosteric agonist (diazepam) modulate electrical charge transfer through GABAAR and the whole-cell excitability. On top of that, diazepam modifies the animal behavioural reaction on startle response. However, despite behavioural reactions displayed by living animals are controlled by GABAARs, changes of pH do not alter them significantly. Possible mechanisms underlying the species resistance to acidification impact are discussed.

Testing olfaction in equids: Age and gestational stage affects olfactory interest

In spite of the highly developed olfactory apparatus of horses, implying a high adaptive value, research on equine olfaction is sparse. Our limited knowledge poses a risk that horse behaviour does not match human expectations. The benefit of acquiring more knowledge of equine olfaction is therefore twofold; 1) it can aid the understanding of horse behaviour and hence reduce the risk of dangerous situations, and 2) there may be unexploited potential of using odours in several practical situations where humans interact with horses. This study investigated olfactory abilities of, 35 Icelandic, horses who were presented to four odours: peppermint, orange, lavender and cedarwood. The response variables were sniffing duration per presentation and behavioural reaction (licking, biting, snorting, and backing). Results showed horses were able to detect and distinguish between all four odours and showed increased interest (significantly longer sniffing duration) for peppermint. More horses expressed licking behaviour when presented to peppermint compared to cedarwood and lavender. Young horses sniffed cedarwood for longer than old horses, and pregnant mares sniffed lavender less than non-pregnant mares. In conclusion, the test paradigm seemed meaningful for horses, and olfactory interest of horses varied with age and gestational status but not sex.

Modelling COVID-19 outbreaks in USA with distinct testing, lockdown speed and fatigue rates

Each state in the USA exhibited a unique response to the COVID-19 outbreak, along with variable levels of testing, leading to different actual case burdens in the country. In this study, via per capita testing dependent ascertainment rates, along with case and death data, we fit a minimal epidemic model for each state. We estimate infection-level responsive lockdown/self-quarantine entry and exit rates (representing government and behavioural reaction), along with the true number of cases as of 31 May 2020. Ultimately, we provide error-corrected estimates for commonly used metrics such as infection fatality ratio and overall case ascertainment for all 55 states and territories considered, along with the USA in aggregate, in order to correlate outbreak severity with first wave intervention attributes and suggest potential management strategies for future outbreaks. We observe a theoretically predicted inverse proportionality relation between outbreak size and lockdown rate, with scale dependent on the underlying reproduction number and simulations suggesting a critical population quarantine ‘half-life’ of 30 days independent of other model parameters.

Using a new video rating tool to crowd-source analysis of behavioural reaction to stimuli

Quantifying the intensity of animals’ reaction to stimuli is notoriously difficult as classic unidimensional measures of responses such as latency or duration of looking can fail to capture the overall strength of behavioural responses. More holistic rating can be useful but have the inherent risks of subjective bias and lack of repeatability. Here, we explored whether crowdsourcing could be used to efficiently and reliably overcome these potential flaws. A total of 396 participants watched online videos of dogs reacting to auditory stimuli and provided 23,248 ratings of the strength of the dogs’ responses from zero (default) to 100 using an online survey form. We found that raters achieved very high inter-rater reliability across multiple datasets (although their responses were affected by their sex, age, and attitude towards animals) and that as few as 10 raters could be used to achieve a reliable result. A linear mixed model applied to PCA components of behaviours discovered that the dogs’ facial expressions and head orientation influenced the strength of behaviour ratings the most. Further linear mixed models showed that that strength of behaviour ratings was moderately correlated to the duration of dogs’ reactions but not to dogs’ reaction latency (from the stimulus onset). This suggests that observers’ ratings captured consistent dimensions of animals’ responses that are not fully represented by more classic unidimensional metrics. Finally, we report that overall participants strongly enjoyed the experience. Thus, we suggest that using crowdsourcing can offer a useful, repeatable tool to assess behavioural intensity in experimental or observational studies where unidimensional coding may miss nuance, or where coding multiple dimensions may be too time-consuming.

Inventář tendence k vině a hanbě (ITVAH): pilotní studie

This paper constitutes a pilot study of a novel measure for assessing proneness to guilt and proneness to shame, Inventář tendence k vině a hanbě (ITVAH, Inventory of Proneness to Guilt and Shame). ITVAH is a scenario-based method distinguishing affective and behavioural components of guilt and shame and also considering the private or public experience of guilt and shame, respectively. Two items (affective and behavioural reaction) were created for each of the 16 scenarios, resulting in total of 32 items. A four-factor measurement model was assumed – Negative behaviour evaluation (NBE), Reparation, Negative self-evaluation (NSE), Withdrawal. Next, we assumed negative correlations of NSE and Withdrawal with self-esteem and self-forgiveness and positive correlations of NBE and Reparation with self-forgiveness. The pilot study was carried out on 249 Czech-speaking adults (67 % women, aged 18–75, Md = 22). An ordinal confirmatory factor analysis with residual covariances between items connected to the same scenarios was conducted. The hypothesized four-factor model was found acceptable. However, we found limited evidence for the hypothesized relationships with self-esteem and self-forgiveness – in contrast to theoretical expectations we found a medium-strong negative relationship between self-forgiveness and affective component of guilt. For further use of the measure in the Czech and Slovak environment it is necessary to assess additional evidence of construct validity and concurrent validity with The Test of Self-Conscious Affect-3 (TOSCA-3).

Rhythmic pattern facilitates speech production: An ERP study

Rhythm affects the speech perception of events unfolding over time. However, it is not clear to what extent the rhythm could affect the processes of sentence speech production. In this event-related potential (ERP) study, we examined whether a particular rhythmic pattern could affect the planning of speech production before articulation. We recorded electrophysiological (EEG) and behavioural (reaction time) data while participants read aloud a target speech in Chinese. Target speeches were sentences or phrases consisting four characters, with regular (e.g., the 2 + 2 pattern; numbers in the brackets represent the number of syllables) or irregular (e.g., 1 + 3) rhythmic patterns, which were preceded by congruent or incongruent musical rhythmic patterns formed by simple pure tones with different temporal intervals. Behavioural and ERP findings indicated a rhythmic priming effect in comparing congruent and incongruent conditions in the regular target speeches, but not in the irregular ones. An early component (N100) that was elicited in response to target speeches that were rhythmically mismatched to primes was linked to the detection of hierarchical linguistic units, which did not conform to expectations. A later negative component (N400) was thought to reflect the violation of expectation on rhythmic pattern in speech production. These findings suggest that rhythmic pattern constrains grammatical and prosodic encoding during speech production, and support the hypothesis that speakers form a grammatical or a prosodic abstract frame before articulation.