Antioxidant and antiviral potency of Begonia medicinalis fractions

Objectives This study aims to evaluate the antioxidant and antiviral potency of n-hexane, ethyl acetate and, water fractions of Begonia medicinalis Ardi & D.C.Thomas as well as to identify the chemical constituents. Methods Assays for antioxidant and antiviral activity (HIV-1) were carried out on MT-4 cells infected with HIV using the DPPH method and the determination of the cytopathic effect. Meanwhile, GC-MS was used to identify the chemical compounds. Results The determination of antioxidants showed that all fractions possessed potent activity with the IC50 ranging from 2.61 to 8.26 μg/mL. From the antiviral activity of MT-4 cells infected by HIV, the n-hexane fraction of B. medicinalis showed the most potency with the IC50 of 0.04 ± 0.05 μg/mL. It has less cytotoxicity (11.08 ± 4.60 μg/mL) affording the high selectivity index of 238.80. Furthermore, GC-MS analysis of n-hexane fraction found the major compound of carboxylic acid derivate with the area percentage of 76.4% and the presence of phenolic compounds (8.38%). Meanwhile, in water fraction, terpenoids were found in a higher concentration (10.05%) than others. Conclusions Therefore, this study supports the application of B. medicinalis as a herbal medicine for antioxidant and antiviral.

Enzymatic Esterification as Potential Strategy to Enhance the Sorbic Acid Behavior as Food and Beverage Preservative

Sorbic acid is the most commonly used preservative in the food industry. The antimicrobial inhibition of sorbic acid could be influenced by its lipophilic nature, which reduces its use in hydrophilic food formulations. Reactions between sorbic acid and glycerol catalyzed by lipases were studied in order to develop a novel sorbic acid derivate with a promising hydrophilic profile. The esterification reaction between sorbic acid and glycerol in a solvent-free system were performed with an immobilized lipase B from Candida antarctica (CALB). The glycerol sorbate product has been tested against S. griseus bacterium and Saccharomyces cerevisiae yeast. Results indicate that the esterification of sorbic acid with glycerol does improve its antimicrobial properties against Saccharomyces cerevisie. The reported results demonstrate that esterification can be used as a strategy to improve the antimicrobial activity of sorbic acid.

Evaluación de la citotoxicidad, genotoxicidad y toxicidad subcrónica de diésteres p-metoxicinámicos extraídos de Copernicia prunifera (Miller) H. E. Moore en modelo experimental

P-methoxycinnamic diesters from carnauba wax powder (PCO - C) – cinnamic acid derivate – have been associated with new properties such as hypolipidemic, hypocholesterolemic and hypoglycaemic effects in mice. The present study aimed to investigate the safety profile of PCO - C by evaluating the subchronic oral toxicity, genotoxicity and mutagenicity using in vivo experimental models. Therefore, for the present research, characterized as experimental and of a quantitative nature, 80 Swiss mice, of both genders, were used for the analysis of mutagenicity, genotoxicity, analysis of the biochemical, hematological and toxicological profiles. PCO - C did not induce changes in the mass of animals besides not interfering in blood parameters. PCO - C showed no hemolysis of mouse erythrocytes. PCO - C caused no mutagenic or genotoxic effects in male and female mice treated with 500 mg/Kg/day during 90 days. In addition, PCO - C did not change some important serum parameters (alanine aminotransferase, aspartate aminotransferase, total cholesterol and creatinine), hematological profile, structural arrangement of the kidneys, heart, stomach and spleen. The results demonstrate that PCO - C is not genotoxic, and did not present toxicity when used orally for up to 90 days. Overall, the study has demonstrated the safety of PCO - C for potential uses in biomedical areas.

Usnic Acid Derivate from Usnea sp. and Bioactivity against Arthemia salina Leach

Usnic acid is chemical compound commonly found in lichen. These derivatives compounds both from the synthesis and from the isolation of various lichens have been studied as antibacterial, antifungal, antiparasitic, antiviral, and anticancer. Usnat acid derivative with not hydroxyl group on carbon C-3 was isolated from lichen usnea sp. taken from the Sinjaiselatan described in this paper. Its bioactivity against Arthemia Salina Leach. LC50 value of 19.49 μg/mL.

Natural Surfactant from Fatty Acid of Morinda citrifolia L. for Deinking Flotation

Natural surfactant is developed as fatty acid derivate from natural resources such as palm oil, sunflower oil, and safflower oil. In South East Asia countries, the palm oil is used as the main resources of fatty acid derivate. Seed oil of Morinda citrifolia L. contains unsaturated fatty acid. It is extracted and concentrated. The concentrated fatty acid of Morinda citrifolia L. (CFAMC) is used as deinking surfactant. The CFAMC is analyzed by GCMS for its constituent and its deinking flotation performance is evaluated for yield, brightness, and ERIC of deinked pulp. Based on the old newspaper (ONP) pulp with brightness of 35.84 °ISO, and ERIC of 1982.4 ppm, the deinking flotation performance of CFAMC is approaching the natural surfactant. The brightness increase is 11.36% and 16.35%, and the ERIC reduction is 37.40% and 47.73% for CFAMC and natural surfactant respectively. SEM analysis is provided to see the residual Nanoink particles on the fibers.

Drug Development of Mefenamic Acid Derivatives as Analgesic by Molecular Approach

A new compound of Mefenamic Acid derivate, 4-nitrobenzoyl-mefenamic acid has been synthesized by benzoylation reaction between mefenamic acid and 4-nitrobenzoyl chloride after prediction by in silico study/molecular approach. A derivative of mefenamic acid (4-NO2-benzoyl-mefenamic acid) has been synthesized for increase its activity as candidate of analgesic drug/inhibitor COX-2 (Cyclooxigenase-2). This compound has been purified by Column Chromatography and analyzed using TLC-Densitometry to determine purity with Rf value 0,8. The spot has good purity and then it was identified this structure using H-NMR 400 MHz and FTIR-KBr. The result showed that this compound is 4-nitrobenzoyl-mefenamic acid (4NBMA). 4NBMA gives white yellow color with melting point 198-199oC. Finally, 4NBMA was tested analgetic activity by hot plate method and it showed that 4-nitrobenzoyl-mefenamic acid has been higher activity than mefenamic acid.