scholarly journals Growth Response of Porphyridium sp. Culture on Glass and Plastic Container in Laboratory Scale

2020 ◽  
Vol 9 (2) ◽  
pp. 155
Author(s):  
Lutfiyah Al Adawiyah ◽  
Mohammad Faizal Ulkhaq ◽  
Hapsari Kenconojati

Utilization of Porphyridium sp. began to be developed as an anticancer, anti-inflammatory, antibacterial, antifungal, and production of biogas and biodiesel so that necessary to carry out culture activities for ensure continuity of Porphyridium sp. This study aims to determine the type of culture container that showed the best growth response of Porphyridium sp. with the highest biomass. The stages in this research were preparation of containers and media sterilization, making diatom fertilizers and agar media, Culture of Porphyridium sp. on agar media, Culture of Porphyridium sp. in the test tube, and Culture of Porphyridium sp. in glass and plastic containers. The results showed that the growth response of Porphyridium sp. that cultured in glass containers (18,9 ± 0,21 x 105 cells/mL) was higher than plastic (15.57 ± 0,03 x 105 cells/ml).

2019 ◽  
Vol 4 (2) ◽  
Author(s):  
Mohammad Faizal Ulkhaq

The aim of this study was to determine the type of culture container for increasing the cell density of Nitzschia and producing the hightly cell biomass. The stages of this study were sterilization of containers and media, making diatom fertilizers and agar media, culture of Nitzschia in agar medium, culture of Nitzschia in test tube, and culture of Nitzschia in glass and plastic containers. The result showed that the cell density of Nitzschia cultured in the glass container was higher than plastic containers. Further study was needed to determine the nutrient composition of Nitzschia that culture in glass containers.


2015 ◽  
Vol 7 (4) ◽  
pp. 460-463 ◽  
Author(s):  
Olamide O. FOLARIN ◽  
Clement O. OGUNKUNLE ◽  
Stephen O. OYEDEJI ◽  
Saheed KOLAWOLE

This study was conducted to evaluate the growth response of three leafy vegetables (Celosia argentea, Amaranthus cruentus and Amaranthus hypochodriacus) to the leaf extract of Vitellaria paradoxa. Forty-five (45) experimental plastic containers were filled with 5 kg of loamy soil each and randomly allocated to the following regimes: control, 20 g, 40 g, 60 g and 80 g of powdered leaves of V. paradoxa, in three (3) replicates for each test crop respectively. It was observed that the response of the three leafy vegetables, grown within different composition of V. paradoxa leaves, are concentration dependent with respect to the studied growth parameters (plant height, leaf number, leaf area, stem girth). This indicated that V. paradoxa has allelopathic potential on the studied vegetables and therefore it could be used for natural weed control.


2014 ◽  
Vol 28 (1-2) ◽  
pp. 35-46
Author(s):  
Daniel Caracanhas Cavallari ◽  
Rodrigo Brincalepe Salvador ◽  
Bruno Rodrigues da Cunha

Abstract Specimen deterioration in museum collections and exhibits is a recurring worry of curators. Malacological collections, i.e., those containing mollusk shells, are of special concern, because these specimens are composed largely of calcium carbonate, a compound that easily deteriorates in acidic storage media. There are two main problems related to the presence of volatile acids in collections: Bynesian decay and pyrite decay. In Bynesian decay, acetic and formic acids, among others, are released by the materials used to store the collection: wood, varnishes, resins, paper, cardboard, and other storage materials. Under high relative humidity and inappropriate temperature, the acids react with the shells' carbonate, yielding salts and carbon dioxide. Pyrite decay is similar in appearance: fossil specimens may contain pyrite (FeS2), which in high relative humidity is oxidized, yielding sulfuric acid and other products. This acid corrodes other shells, including carbonatic fossils. The damage caused is irreversible, but there are methods to avoid or mitigate it. The collection should be kept in conditions of low relative humidity (45–50%) and appropriate temperature range (16–21°C). The use of archival materials, such as steel drawers and cabinets with electrostatic coating, is mandatory. Specimens containing reactive pyrite must be kept in archival-quality plastic containers or closed glass containers. Damaged specimens must be immediately removed and only returned to the collection after proper cleaning.


1972 ◽  
Vol 50 (3) ◽  
pp. 683-685
Author(s):  
R. V. Clark

Four isolates of C. sativus were able to use a number of carbon sources to varying degrees with little evidence of a differential growth response by the isolates. With most carbon sources the response was different when growth on liquid media was compared with that on agar media. Dextrin and L-sorbose were exceptions as dextrin supported good growth with both types and L-sorbose poor with both. Lactose supported the best mycelial growth on liquid media and dextrin the best radial growth and sporulation on agar media when compared with sucrose.


Author(s):  
Laura A Canty ◽  
Roger Lewis ◽  
Alaster Yoxall

As society ages there is a growing need to understand issues surrounding declining user capabilities. One such area has been accessibility of packaging by older people. To date much of the current research in this area has focused on measuring strength of older people and analysing the force needed to open various pack formats and has largely concentrated on accessibility of jam or sauce jars. However, a survey by ‘Yours’ magazine indicated that problems with thin film pack forms and peelable packaging was also an issue for older people. Hence the authors undertook a small-scale study to understand the issues surrounding accessing a rigid plastic container with a peelable lid. To that end the authors built a bespoke measuring device to measure container peeling forces, measured finger friction between pack and finger and undertook an observational analysis on 60 users accessing packaging of this type. Results indicated that the force needed to open containers of this type is lower than measured user forces including older people and it is therefore likely that the issues surrounding accessibility of this type of pack format are related to dexterity not strength. However, the authors also showed that this can be affected by context of use in that oily fingers could reduce the friction coefficient between finger and pack such that older people may not have sufficient strength to open packs of this type.


Author(s):  
K. Eden Luboya ◽  
Mélissa Kusisakana ◽  
W. Gaston Luhata ◽  
K. Balthazar Mukuna ◽  
M. Justine Monga ◽  
...  

This experiment was conducted at the Bioenergy laboratory of Groupe de Génies Congolais (GGC) at the Université Loyola du Congo in Kinshasa, D.R.Congo. The experiment started on May 23, 2019 and ended on July 17, 2019. The study focused on the relationship between solids concentration and the kinetic of anaerobic digestion of goat droppings in the methanation process. The feedstock consisted of goat droppings waste made into slurry of four solids concentration (SC); A=50%, B=38%, C=30% and D=25%. Each SC was repeated three times. Feedstocks were inserted in laboratory scale anaerobic digesters constructed from 5L plastic containers. The results revealed clearly that the time required for the production of biogas depends on SCs. The ratio D (1/3), i.g. 75% of water and 25% of biomass produced the biogas in 3 weeks (±22 days) and the ratio A (1/1), 50% of water and 50% of biomass, in 7 weeks and a few days (±53 days). The ratio C (1/2), 66.6% of water in the mixture, provided the biogas in ± 26 days (approximately 4 weeks) and finally, it took ±30 days (4 weeks and a few days) for ratio B to produce biogas rich in CH4 (<50%). The equation f(x)= 1.1x + 1 can be used to predict the approximative number of days to produce a biogas containing more than 50% of CH4. The average temperature inside the reactors was found to be 28.5 ± 0.8°C during the combustion testing process implying that the reactors designed at the GGC were operating in a mesophilic regime. Finally, the pH of the digestates obtained from reactors had an average of 9.0 ± 0.2.


BioScience ◽  
2019 ◽  
Vol 3 (2) ◽  
pp. 121
Author(s):  
Alfi Rizca Hardianti ◽  
Helga Lusiana ◽  
Dita Widayanti Sawitri

This study aims to understand the growth response of indigenous bacteria originating from oil sludge in Dumai which has the potential as Naphthalene and Fenantren degrading agents based on variations in substrate pH. The isolates used in this study were Isolate A, Isolate E, and Isolate F as a result of isolation from oil sludge in Dumai. Based on the results of the growth response test on various concentration in previous studies, we chose E isolates to be tested for its degradation ability. The bacterial growth response data was obtained based on OD measurements every 24 hours using a spectrophotometer and TPC at 72 hours using Nutrient Agar media. Based on these data, we determined the optimum pH for indigenous bacterial growth potential to degrade naphthalene or phenanthrene. In all variations of pH, bacterial isolate E experienced growth. This shows that bacterium E can use naphthalene and fenantren at normal pH range (pH 5-9).


1995 ◽  
Vol 5 (1) ◽  
pp. 48-49
Author(s):  
E.F. Gilman ◽  
R.J. Beeson

The root : shoot ratio for Ilex cassine L. grown 7 months in copper-treated containers was less than in nontreated containers. There was less dry weight for roots <5 mm in diameter in copper-treated containers than in nontreated containers in the outer 1 cm of the rootball. Dry weight of roots >5 mm in diameter within the rootball were not affected by copper hydroxide treatment. Coating the interior of a plastic container with cupric hydroxide eliminated coarse roots (> 5 mm in diameter) and significantly reduced fine root weight from the outer 1 cm of the rootball. Fine roots inside the rootball did not replace fine roots lacking in the outer 1 cm.


Mycobiology ◽  
2001 ◽  
Vol 29 (3) ◽  
pp. 164-169 ◽  
Author(s):  
U. P. Singh ◽  
B. K. Sarma ◽  
Ruo Nishimura ◽  
Kiroku Kobayashi ◽  
Akira Ogoshi ◽  
...  

2019 ◽  
Vol 43 (2) ◽  
Author(s):  
Patrícia Gibbert ◽  
Marlene de Matos Malavasi ◽  
Ubirajara Contro Malavasi ◽  
João Alexandre Lopes Dranski

ABSTRACT Studying the physiological and biochemical behavior of Myrcianthes pungens seeds stored in different packages for up to ten months was the objective of this work. Seeds were collected in Toledo, Pato Bragado, and Marechal Cândido Rondon and stored in a cold and dry chamber (11 ºC and 6,3% RH) for ten months. The germination test and the germination speed index (GSI), the seedling length (SL), the seedling dry matter mass (DMS), and the tetrazolium (ZT) test were performed and, for each evaluation, the water content was determined. Non-parametric analysis was used. During storage, the water content of the seeds in the plastic and glass containers varied little, but decreased in the paper packaging. The germination was maintained for ten months when the seeds were stored in plastic containers and for two months in the glass and paper ones. The variables GSI, SL, and DMS presented upwards and downwards variations during the storage period. The ZT revealed that, in the plastic packaging, the seeds remained viable for up to ten months, in glass, up to two months, and in paper, up to four months. Therefore, seeds of Myrcianthes pungens with high initial quality can be stored in a cold and dry chamber in plastic bags, maintaining viability and vigor for a period of ten months


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