ON IMPACT OF THE BONE TYPE ON THE RESULTS OF PALEODIET RECONSTRUCTIONS ON THE BASIS OF THE STABLE ISOTOPE ANALYSIS

Анализ стабильных изотопов азота и углерода активно применяется в палеодиетических реконструкциях археологического материала. Однако на результаты изотопного исследования могут повлиять существующие отличия в соотношениях изотопов разных типов костей одного индивида, которые могут возникать из-за разницы в скорости ремоделирования (разрушение старой кости и образование новой) различных костей скелета. Данные отличия часто не учитываются при выборе образцов для проведения изотопного исследования. Таким образом, перед настоящей статьей ставятся следующие задачи. Во-первых, показать существование разницы в изотопных соотношениях костей скелета на основе материалов, представленных древнерусским сельским населением из могильника Шекшово 9 в Суздальском Ополье (X-XII вв.). И, во-вторых, предоставить основные рекомендации при отборе проб для изучения изотопного состава археологических материалов. В ходе исследования удалось установить, что при выборе образцов для проведения изотопного анализа следует отдавать предпочтения костям со схожей скоростью ремоделирования. The analysis of stable nitrogen and carbon isotopes is actively used in paleodiet reconstructions of archaeological materials. However, the results of the stable isotope study can be affected by differences in the isotope ratios of collagen in different types of bones of the same individual. This can arise due the different speed of remodeling (the process by which osteoclasts breakdown the tissue in bones and new bones form) of various bones in the skeleton. These differences are often disregarded when selecting samples for isotope studies. Therefore, this paper is intended to address the following tasks: (i) to show that there exists difference in the isotope ratios of the skeleton bones by analyzing human remnants of the Medieval Russia rural population from the Shekshovo-9 cemetery in Suzdal Opolye (10th-12th centuries); (ii) to provide basic recommendations for selecting samples for the studies of the stable isotope composition of archaeological materials. Our research evidences that. when selecting samples for the isotope analysis. preference should be given to the bones with similar remodeling speed.

Stable Isotope Analysis of Ozark Hellbender (Cryptobranchus alleganiensis bishopi) Living and Preserved Museum Tissue Reveals a Shift in Their Generalist Diet Composition

Ozark hellbenders (Cryptobranchus alleganiensis bishopi) have undergone marked population declines across their entire distribution. A variety of ecological life history research has been conducted to determine the cause(s) of the declines. Historically, hellbender diet studies used stomach content examination methods; however, alternative approaches such as less intrusive stable isotope analyses are now options for researchers. The goals of our study were to conduct stable isotope analysis on live and formalin-preserved museum specimen Ozark hellbender tissues to identify diet composition in the Eleven Point and Spring rivers, Arkansas. Also, we used stable isotope analysis to investigate if Spring River hellbender diets have changed over time. We sampled fish, live hellbenders (non-destructively), and formalin-preserved hellbender tissues from museum collections for stable isotope analysis. We sampled crayfish for assemblage composition and stable isotope analysis. The results of our stable isotope study revealed three main findings: (1) there were no statistically significant differences between hellbender δ13C and δ15N values among sites and hellbender stable C and N isotopes were correlated with body length; (2) traditional δ13C versus δ15N bi-plots and trophic discrimination values did not provide complete discernment in hellbender diets; however, Bayesian MixSIAR models revealed hellbenders to be generalists, and (3) the use of δ13C and δ15N values adjusted historic formalin-fixed and ethanol preserved hellbenders matched well with current crayfish and fish stable isotope values based on Bayesian MixSIAR models. These findings provide important diet information and a possible tool to examine dietary patterns from preserved specimens that may be used for hellbender conservation and management.

Ontogenetic shift in the trophic role of the invasive killer shrimp Dikerogammarus villosus: a stable isotope study

The introduction of the amphipod Dikerogammarus villosus in European fresh waters is to date recognized as a threat to the integrity of invaded communities. Predation by D. villosus on native benthic invertebrates is assumed as the key determinant of its ecological impact, yet available information describe the species as a primary consumer as well as a carnivore depending on local conditions. Here, we assessed the trophic position (TP) of D. villosus in Lake Trasimeno, a recently invaded lentic system in central Italy, using the CN isotopic signatures of individuals captured in winter spanning two orders of magnitude in body size. TP estimations were compared with those characterizing the native amphipod Echinogammarus veneris and other representative invertebrate predators. On average, D. villosus showed a trophic position higher than E. veneris, and comparable with that of odonate nymphs. An in-depth analysis revealed that large-sized individuals had a trophic position of 3.07, higher than odonates and close to that of the hirudinean predator Erpobdella octoculata, while small-sized specimens had a trophic position of 2.57, similar to that of E. veneris (2.41). These findings indicate that size-related ontogenetic shifts in dietary habits may per se vary the nature of the interaction between Dikerogammarus villosus and native invertebrates from competition to predation. Information collated from published isotopic studies corroborated the generality of our results. We conclude that intra-specific trophic flexibility may potentially amplify and make more multifaceted the impact of the species on other invertebrate species in invaded food webs.

Effect of fasting and feeding on apolipoprotein A-I kinetics in preβ1-HDL, α-HDL, and triglyceride-rich lipoproteins

The aim of this study was to compare the kinetics of apolipoprotein (apo)A-I during fed and fasted states in humans, and to determine to what extent the intestine contributes to apoA-I production. A stable isotope study was conducted to determine the kinetics of apoA-I in preβ1 high-density lipoprotein (HDL) and α-HDL. Six healthy male subjects received a constant intravenous infusion of 2H3-leucine for 14 h. Subjects in the fed group also received small hourly meals. Blood samples were collected hourly during tracer infusion and then daily for 4 days. Tracer enrichments were measured by mass spectrometry and then fitted to a compartmental model using asymptotic plateau of very-low-density lipoprotein (VLDL) apoB100 and triglyceride-rich lipoprotein (TRL) apoB48 as estimates of hepatic and intestinal precursor pools, respectively. The clearance rate of preβ1-HDL-apoA-I was lower in fed individuals compared with fasted subjects (p < 0.05). No other differences in apoA-I production or clearance rates were observed between the groups. No significant correlation was observed between plasma apoC-III concentrations and apoA-I kinetic data. In contrast, HDL-apoC-III was inversely correlated with the conversion of α-HDL to preβ1-HDL. Total apoA-I synthesis was not significantly increased in fed subjects. Hepatic production was not significantly different between the fed group (17.17 ± 2.75 mg/kg/day) and the fasted group (18.67 ± 1.69 mg/kg/day). Increase in intestinal apoA-I secretion in fed subjects was 2.20 ± 0.61 mg/kg/day. The HDL-apoA-I kinetics were similar in the fasted and fed groups, with 13% of the total apoA-I originating from the intestine with feeding.

Mid-Holocene palaeohydrochemistry and palaeohydrology of Yamdrok Yumtso, southern Tibetan Plateau, reconstructed from δ18O and δ13C of fossil shells of the gastropod Radix auricularia

Palaeohydrochemical and palaeohydrological changes of lakes have seldom been reconstructed from the fossil shells of the gasropod Radix auricularia, which is a new, and potentially high-resolution environnmental archive. We conducted a geochemical and stable isotope study of the shells of Radix from the exposed fluvio-lacustrine sediments near Lake Yamdrok Yumtso in the southern Tibetan Plateau. Our aims were to determine the sedimentary environment, palaeo-lake hydrochemistry and hydrological status. AMS 14C and OSL dating indicates that a lake-level stage of Yamdrok Yumtso higher than that of today occurred during ~4.7–1.2 cal. kyr BP. Results of Sr/Ca, δ13C and δ18O analysis of the fossil shells of Radix auricularia indicate that the lake-level fluctuations were mainly controlled by changes of the Indian Summer Monsoon; decreasing evaporation during the mid- to late-Holocene was also responsible. In addition, based on the geochemical relationship between Radix sp. shells and the ambient water in lakes, the values of δ18OPalaeo-water and Sr/CaPalaeo-water reconstructed using the fossil shells of Radix auricularia are −8.2‰ to −5.1‰ and 0.0012 to 0.0057, respectively. Further, based on the values of δ18Oshell, together with geomorphological evidence, we infer that Yamdrok Yumtso was a closed lake system, and we estimate its possible extent during the interval of high lake-level. In addition, we speculate that the water level of Yamdrok Yumtso at this time exceeded 4448.9 m a.s.l., but was less than 4451 m a.s.l., and that the major separation of various components of the Yamdrok Yumtso system occurred after 1.2 kyr BP.