The slower antibody response in myelofibrosis patients after two doses of mRNA SARS-CoV-2 vaccine calls for a third dose

Immunization with mRNA SARS-CoV-2 vaccines has been highly recommended and prioritized in fragile categories with higher risk of mortality after COVID-19 disease compared to healthy people, including patients with myelofibrosis (MF). Available data on the vaccine immune re-sponse developed by MF patients, and the impact of the treatment with the inhibitor of JAK-STAT signaling ruxolitimib, are still fragmented to support an informed decision for a third dose for this category of subjects. Here, we show that 76% of MF patients develop spike-specific IgG after the second vaccine dose, but the response has a slower kinetic compared to healthy subjects, suggesting a reduced capability of their immune system to promptly react to vaccina-tion. A reduced ACE2/RBD inhibition binding activity of spike-specific antibodies was also ob-served, especially in ruxolitimib treated patients. Our results contribute to answer the open question on the induction of the antibody responses in MF patients following vaccination with COVID-19 mRNA vaccines, showing a slow kinetic that support the need for a third dose of SARS-CoV-2 mRNA vaccines.

In vivo evidence for the unique kinetics of evoked dopamine release in the patch and matrix compartments of the striatum

The neurochemical transmitter dopamine (DA) is implicated in a number of diseases states, including Parkinson’s disease, schizophrenia, and drug abuse. DA terminal fields in the dorsal striatum and core region of the nucleus accumbens in the rat brain are organized as heterogeneous domains exhibiting fast and slow kinetic of DA release. The rates of dopamine release are significantly and substantially faster in the fast domains relative to the slow domains. The striatum is composed of a mosaic of spatial compartments known as the striosomes (patches) and the matrix. Extensive literature exists on the spatial organization of the patch and matrix compartments and their functions. However, little is known about these compartments as they relate to fast and slow kinetic DA domains observed by fast scan cyclic voltammetry (FSCV). Thus, we combined high spatial resolution of FSCV with detailed immunohistochemical analysis of these architectural compartments (patch and matrix) using fluorescence microscopy. Our findings demonstrated a direct correlation between patch compartments with fast domain DA kinetics and matrix compartments to slow domain DA kinetics. We also investigated the kinetic domains in two very distinct sub-regions in the striatum, the lateral dorsal striatum (LDS) and the medial dorsal striatum (MDS). The lateral dorsal striatum as opposed to the medial dorsal striatum is mainly governed by fast kinetic DA domains. These finding are highly relevant as they may hold key promise in unraveling the fast and slow kinetic DA domains and their physiological significance. Graphical abstract

Static and Dynamic Investigations on Leaching/Retention of Nutrients from Raw Poultry Manure Biochars and Amended Agricultural Soil

In this study, nutrients release/adsorption from/by raw poultry manure-derived biochar produced at a pyrolysis temperature of 600 °C (RPM-B) was assessed under static and dynamic conditions. Batch sequential leaching experiments of RPM-B for a total contact time of 10 days showed that both phosphorus and potassium were slowly released but with higher amounts compared to various other animal- and lignocellulosic-derived biochars. The cumulated released P and K amounts were assessed to 93.6 and 17.1 mg g−1, which represent about 95% and 43% of their original contents in the RPM-B, respectively. The column combined leaching/adsorption experiments showed that amending an alkaline sandy agricultural soil with two doses of RPM-B (at 5% and 8% w:w) resulted in an efficient retention of NO3-N and NH4-N, and on the contrary, important leached amounts of PO4-P, K+, Mg2+, and Ca2+ but with relatively slow kinetic release rates for a long period. Even after 40 days of dynamic leaching, these latter nutrients continued to be released with kinetic rates lower than 10 mg kg−1 d−1. Thus, compared to synthetic fertilizers, RPM-B valorization as organic amendment for poor semiarid soils could be considered as an attractive, eco-friendly, and sustainable waste recycling option.

The influence of acoustic power on chemical absorption of CO2 using Slow Kinetic Solvent

Ultrasonic Irradiation (UI) is an emerging technology that is used to assist the CO2 absorption process. Even for the slow kinetic solvents without using any chemical promoter, high-frequency UI might enhance mass transfer during the absorption process. For this purpose, it is essential to study the performance of a high-frequency ultrasonic-assisted absorption system under varied operating conditions. The ultrasonic power is considered as one of the main parameters during the absorption of CO2. Thus, in this paper, the influence of ultrasonic power is presented using Methyl diethanolamine (MDEA) as a chemical solvent. The ultrasonic power has been varied from 0 to 15.3 W. The results tend to show a significant absorption rate enhancement for higher ultrasonic power. Moreover, they prove that the high-frequency ultrasonic absorption system has high potential to be utilized to enhance the absorption using promoter-free MDEA.

Improvement of hydrogen storage characteristcs of MgH2 with flake Ni nano-catalyst composite

Magnesium hydride (MgH2) is considered as one of the most promising hydrogen storage materials due to its safety, low cost and high hydrogen storage capacity. However, its slow kinetic performance...

Slow kinetic evolution of nanohelices based on gemini surfactant self-assemblies with various enantiomeric excess; chiral segregation towards a racemic mixture

Very slow kinetic evolution (days) of chiral nanoribbons based on amphiphilic molecular assemblies is investigated for various enantiomeric excess.

HYDROTHERMAL SYNTHESIS OF NANO BILAYERED V2O5 AND ELECTROCHEMICAL BEHAVIOR IN NON–AQUEOUS ELECTROLYTES LiPF6 AND NaClO4

This work aimed to prepare bilayered V2O5 by hydrothermal route from vanadium (III) chloride (VCl3). According to XRD results, bilayered V2O­5 showed a large interlayer spacing around 11.3 Å. The electrochemical properties of bilayered V2O5 were carried out by cyclic voltammetry and charge–discharge testing in non–aqueous electrolytes LiPF6 and NaClO4. The curves charge–discharge showed that mechanism of insertion/extraction of Li+ ions and Na+ ions were occurred on a solution solid without the phase transition. Moreover, specific capacity for lithium and sodium intercalation of bilayered V2O5 were found out 250 mAh/g and 200 mAh/g, respectively. The kinetic of lithium’s and sodium’s insertion was evaluated by the electrochemical impedance spectroscopy (EIS). The EIS results exhibited a stabilization of charge transfer in both case and a slow kinetic of sodium’s diffusion compared to lithium’s case due to the large ionic radius of sodium.

New synchrotron powder diffraction facility for long-duration experiments

A new synchrotron X-ray powder diffraction instrument has been built and commissioned for long-duration experiments on beamline I11 at Diamond Light Source. The concept is unique, with design features to house multiple experiments running in parallel, in particular with specific stages for sample environments to study slow kinetic systems or processes. The instrument benefits from a high-brightness X-ray beam and a large area detector. Diffraction data from the commissioning work have shown that the objectives and criteria are met. Supported by two case studies, the results from months of measurements have demonstrated the viability of this large-scale instrument, which is the world's first dedicated facility for long-term studies (weeks to years) using synchrotron radiation.

The influence of fast waves and fluctuations on the evolution of the dynamics on the slow manifold

The effect of non-slow (typically fast) components of a rotating stratified Boussinesq flow on the dynamics of the slow manifold is quantified using a decomposition that isolates the part of the flow living on the slow manifold. In this system, there are three distinct asymptotic limits with corresponding reduced equations, each defining a slow manifold. All three of these distinct limits, namely rapid rotation, strong stratification, and simultaneous strong stratification and rapid rotation (quasi-geostrophy), are considered. Numerical simulations indicate that, for the geometry considered (triply periodic) and the type of forcing applied, the fluctuations act as a conduit, moving energy onto the slow manifold. This decomposition clarifies how the energy is exchanged when either the stratification or the rotation is weak. In the quasi-geostrophic limit, most of the energy transfer is between slow potential energy and slow kinetic energy, but the energetics due to the fluctuations are less clear. It is observed that the energy off the slow manifold in each case equilibrates to a quasi-steady value.

Kinetic behaviour of DPPH radical scavenging activity of tomato waste extracts

The kinetic behaviour of tomato waste extracts (obtained from six genotypes) and standard antioxidant compounds (ascorbic and caffeic acid) were investigated using 2,2-diphenyl-1-picrylhydrazyl radical test. Based on the time needed for the reaction to reach steady state, the investigated extracts show very slow (steady state ? 180 min) antiradical behaviour, ascorbic acid act as rapid antioxidant (steady state < 5min) while caffeic acid is rapidintermediate antioxidant (5 min < steady state < 20 min). The efficient concentrations at different kinetic times EC50,t were determined for all extracts, ascorbic and caffeic acid. EC50,t was used as a parameter to screen and compare antiradical activities of food extracts with slow kinetic action. Irrespective of the time considered, the comparison of the EC50,t for extracts showed that the DPPH radicals scavenging activity of extracts decreased in the order of O2 > Knjaz > Backa > Saint Pierre > Rutgers > Novosadski niski. Tomato waste extracts showed very slow kinetic action, which is probably result of the different kinetic bevaviour of phenolic compounds, present in tomato waste, as well as other antioxidants (vitamins, carotenoids etc).