miR-140-5p Affects TCC8113 Cell Biological Activity and Matrix Metalloproteinases 9 (MMP-9) in Oral Squamous Cell Carcinoma (OSCC) Cell Line

2021 ◽  
Vol 11 (12) ◽  
pp. 2449-2453
Author(s):  
Shaobin Liu ◽  
Zichen Liu ◽  
Changjuan Meng ◽  
Cuili Zhang ◽  
Wei Yu
Keyword(s):  
Biological Activity ◽  
Western Blot ◽  
Cell Viability ◽  
Cell Line ◽  
Negative Control ◽  
Cell Transfection ◽  
Mtt Method ◽  
Significant Difference ◽  
Matrix Metalloproteinases 9 ◽  
Tca8113 Cell

Whether the miR-140-5p affects the biological activity of Tca8113 cells and MMP-9 in OSCC cell line was explored. Tca8113 cells were divided into Tc group (normal Tca8113 cells), Tm group (Tca8113 cell transfection+miR-140-5p mimic), and Nc group (Tca8113 cell transfection+miR-140-5p-negative control) followed by analysis of MMP-9 expression by western blot, cell migration by Transwell assay, cell viability by MTT method, and apoptosis by flow cytometry. Compared with TC group and NC group, mir-140-5p in TM group was significantly higher (P <0.05), however, the MMP-9 level of TM group was significantly lower (P <0.05).Western blot analysis showed that there was no significant difference (P <0.05) in the comparison of MMP-9 protein expression between TC group and NC group, and MMP-9 expression in the TM group was decreased significantly (P <0.05). MTT assay showed that the cell viability of TM group was increased slowly and lower than that of group (P <0.05). In conclusion, mir-140-5p can induce apoptosis of OSCC cell line Tca8113, which may be achieved by reducing the expression of MMP-9.

Antineuroinflammation activity of n-butanol fraction of Marsilea crenata Presl. in microglia HMC3 cell line

2020 ◽  
Vol 30 (6) ◽  
Author(s):  
Burhan Ma’arif ◽  
Denis Mery Mirza ◽  
Mu’akibatul Hasanah ◽  
Hening Laswati ◽  
Mangestuti Agil
Keyword(s):  
Cell Line ◽  
Laser Scanning ◽  
Positive Control ◽  
Confocal Laser Scanning Microscope ◽  
Negative Control ◽  
Confocal Laser ◽  
Classical Pathway ◽  
Butanol Fraction ◽  
Mhc Ii ◽  
Significant Difference

AbstractBackgroundNeuroinflammation is one of the main causes of neurodegenerative events. Phytoestrogen is a group compounds that have an estrogen-like structure or function. Phytoestrogen has a high potential to overcome neuroinflammation caused by estrogen deficiency in postmenopausal women. Marsilea crenata Presl. is a plant known to contain phytoestrogens. This research aimed to analyze the activity of an n-butanol fraction of M. crenata leaves in inhibiting the classical pathway activation of microglia HMC3 cell line to M1 polarity, which has proinflammatory characteristics.MethodsMicroglia HMC3 cell line was cultured in Eagle’s minimum essential medium and induced with IFN-γ for 24 h to activate the cell to M1 polarity in 24-well microplates. The n-butanol fraction was added with various doses of 62.5, 125, and 250 ppm and genistein 50 μM as a positive control. The expression of major histocompatibility complex II (MHC II) as a marker was tested using a confocal laser scanning microscope.ResultsThe result of MHC II measurement shows a significant difference in the MHC II expression in the microglia HMC3 cell line between the negative control and all treatment groups at p<0.05, indicating a non-monotonic dose-response profile.ConclusionsThe best dosage to inhibit MHC II expression was 250 ppm with the value of 200.983 AU. It is then concluded that n-butanol fraction of M. crenata leaves has antineuroinflammation activity due to its phytoestrogens.

scholarly journals A Preliminary Research Study on the Cytotoxicity of Expired and Non-expired Composite Resins: In Vitro Study

2020 ◽  
pp. 227-238
Author(s):  
Magrur Kazak DDS, PhD ◽  
Nazmiye Donmez DDS, PhD ◽  
Fatemeh Bahadori PhD ◽  
Vildan Betul Yenigun PhD ◽  
Abdurrahim Kocyigit MD, PhD
Keyword(s):  
Cell Viability ◽  
Composite Resin ◽  
In Vitro Study ◽  
Negative Control Group ◽  
Negative Control ◽  
Composite Resins ◽  
Control Group ◽  
Significant Difference ◽  
Preliminary Research

Objective: Studies have focused on use of non-expired composites. Unfortunately some clinicians still use expired composite resins without considering their effects. The objective of this in vitro preliminary research was to investigate cytotoxicity of expired(6-months) and non-expired composite resins. Materials and methods: Expired (E) and non-expired (NE) samples of one bulk-fill (Tetric N-Ceram Bulk-fill [TNB], Ivoclar Vivadent), two nano-hybrid (Tetric N-Ceram [TN], Ivoclar Vivadent; Clearfil Majesty ES-2 [CM], Kuraray) composite resins were tested on L929 fibroblast cells. Medium covering cells was removed then plastic rings (2-mm height) were filled with non-polymerized composite resins, placed in direct contact with cells and polymerized with LED light curing unit (LCU). Three samples were prepared for each group. After polymerization, removed medium was added to the cells. Cells that were left without medium (WOM) and cells that were exposed to LCU were used as positive control groups. Cells without any treatment were used as negative control group (C). Cells were incubated with tested materials for 7-days to evaluate cytotoxicity. Cell viability was calculated by sulforhodamine B test as a percentage (%). One-way ANOVA and post-hoc Tukey tests were used for statistical analyses (p<0.05). Results: Comparison between E and NE groups of same composite resins did not result in statistically significant differences (p>0.05), except between TN NE and TN E (p<0.05). TN E group was significantly more cytotoxic than TN NE group. When NE composite resin groups were compared to each other, statistically significant difference was only obtained between TNB NE and TN NE (p<0.05). Among all tested groups, TN NE group showed the least cytotoxic profile. No statistically significant differences were determined when E composite resin groups were compared to each other (p>0.05). All experimental groups compared with C group showed statistically significant cytotoxicity (p<0.05). A statistically significant difference existed between LCU and C groups (p<0.05). Conclusions: In clinical practice, expired composite resins should never be used. Although a correlation was found between expiration dates of nano-hybrid composite resins and cell viability, opposite data were obtained for bulk-fill composite resin. Researches are still required to evaluate biocompatibility of bulk-fill composite resins at various thicknesses with current LCUs.

scholarly journals Hepatoprotective activity of Flacourtia jangomas (Lour.) Raeuschleaves and fruit methanolic extract on paracetamol-induced hepatotoxicity in HepG2 Cells

Biomedicine ◽  
2021 ◽  
Vol 41 (3) ◽  
pp. 587-591
Author(s):  
Akshaya Pai ◽  
Chandrakala Shenoy
Keyword(s):  
Cell Viability ◽  
Cell Line ◽  
Hepg2 Cell ◽  
Hepg2 Cells ◽  
Methanolic Extract ◽  
Hepatoprotective Activity ◽  
Negative Control ◽  
Hepg2 Cell Line ◽  
Fruit Extract ◽  
Deciduous Fruit

Introduction and Aim: Plants have become the current focus of research in treating the various diseases and ailments. Flacourtia jangomas (Lour.) Raeusch belongs to the familySalicaceae. Itis a small deciduous fruit tree having immense nutritional and medicinal significance. Different parts of the plant are pharmaceutically used forcuring various ailments. In this study, we investigated the hepatoprotective activity of Flacourtia jangomas (Lour.) Raeusch leaves and fruit methanolic extract on Paracetamol induced HepG2 cell line.   Methods: The cytotoxic and hepatoprotective properties were evaluated by measuring cell viability; activities of aspartate aminotransferase (AST), alanine aminotransferase (ALT), and lactate dehydrogenase (LDH); lipid peroxidation (malondialdehyde (MDA) levels).   Results:The increased cell viability of 140.43± 4.07% and 133.93±3.20%was observed in HepG2 cells treated with methanolic extract of F. jangomas leaf and fruit extract respectively at 10µg/ml concentration and then decreased along with the rise of F. jangomas leaf and fruit extract concentrations. The level of LDH, ALT, AST and MDA decreased after F. jangomas leaf and fruit treatment compared to negative control.   Conclusion: This study suggests that the methanolic Extract of F. jangomas (Lour.) Raeusch leaves(FJL)and fruit (FJF) shows hepatoprotective activity in Paracetamol induced HepG2 cell line by the decrease in AST and ALT activities and LDH and MDA level. Hence, it could be considered as a therapeutic agent in curing liver-related diseases.  

scholarly journals Toxic Potentials of Methamphetamine in Neuroblastoma Cell Line and its Effects on the Mitochondrial Activity

2021 ◽  
pp. 16-26
Author(s):  
Xolisile Mokoena ◽  
Olufemi A. Alamu
Keyword(s):  
Cell Viability ◽  
Cell Line ◽  
Neuroblastoma Cell ◽  
Recovery Period ◽  
Normal Growth ◽  
Neuroblastoma Cell Line ◽  
Mitochondrial Activity ◽  
Xtt Assay ◽  
Human Neuroblastoma ◽  
Significant Difference

The cytotoxicity potentials of methamphetamine (METH) is presumably associated with oxidative stress induced apoptosis, this study therefore, investigated the toxic potentials of METH in neuroblastoma cells and further determined it effects on the mitochondrial activity. Human neuroblastoma SK-N-BE (2) cells cultured in DMEM/F12 were used in this study. The cells were treated acutely with methamphetamine (1, 5, 10, 20, and 50 µg/mL) over 24, and were allowed to recover from METH treatment over 48, 72, and 96 h. Cell viability study was done with Trypanblue exclusion assay. The cell proliferative characteristics of the neuroblastoma cell line were investigated by constructinga cell proliferation curve. Mitochondrial activity was assessed using the XTT Assay. Statistical analysis were done with Graph Pad prism and significant difference were considered at p<0.001, 0.01 and 0.05. The result showed normal growth in the untreated neuroblastoma cell over the 96 h of monitoring. Following treatment with METH, significant decrease in cell growth was observed when treated acutely with 5 and 10 µg/mL METH and allowed till 72 and 96 h recovery period. The SK-N-BE (2) treated with increasing concentration of METH showed no significant difference in cell viability over the recovery period from METH exposure. Toxicity of SK-N-BE (2) cells was only observed when treated with 10 µg/mL of METH. Significant decrease in mitochondria activity was observed when the cells were treated with 5, 10, 20, and 50 µg/mL METH and allowed till 72 h recovery. The result showed that METH is cytotoxic to the SK-N-BE (2) cells and the mechanism of toxicity might be associated with inhibition of mitochondrial activity.

A New Storage Medium for an Avulsed Tooth

2008 ◽  
Vol 9 (6) ◽  
pp. 25-32 ◽  
Author(s):  
Abbas Ali Khademi ◽  
Saeed Saei ◽  
Mohammad Reza Mohajeri ◽  
Nooshin Mirkheshti ◽  
Fatima Ghassami ◽  
...  
Keyword(s):  
Cell Viability ◽  
Tap Water ◽  
Egg White ◽  
Positive Control ◽  
Negative Control ◽  
Subsequent Treatment ◽  
Blue Staining ◽  
Storage Medium ◽  
Pdl Cells ◽  
Significant Difference

Aim The purpose of this study is to determine the efficacy of egg white in maintaining the viability of human periodontal ligament (PDL) cells on avulsed teeth. Methods and Materials The experimental media were: egg white, milk, Hanks’ Balanced Salt Solution (HBSS) as the positive control, and tap water as the negative control. The storage times were 1, 2, 4, 8, and 12 hours. Extracted premolar teeth of healthy individuals were rinsed in the media. After trypsinization and subsequent treatment in collagenase, cell viability was determined using trypan blue staining. The two-way analysis of variance (ANOVA) statistical test was used to compare the results among different media. Results There was no difference in the cell viability between egg white and HBSS media, but there was a statistically significant difference between the viability of PDL cells in egg white medium in comparison with milk (P<0.05) and water (P<0.05). Conclusion Egg white could be suggested as a suitable storage medium. Its principle advantage is its availability. Citation Khademi AA, Saei S, Mohajeri MR, Mirkheshti N, Ghassami F, Torabi nia N, Alavi SA. A New Storage Medium for an Avulsed Tooth. J Contemp Dent Pract 2008 September; (9)6:025-032.

scholarly journals Monocyte–macrophage ferric reductase activity is inhibited by iron and stimulated by cellular differentiation

1998 ◽  
Vol 336 (3) ◽  
pp. 541-543 ◽  
Author(s):  
Jason PARTRIDGE ◽  
Daniel F. WALLACE ◽  
Kishor B. RAJA ◽  
James S. DOOLEY ◽  
Ann P. WALKER
Keyword(s):  
Cell Line ◽  
Iron Metabolism ◽  
Reductase Activity ◽  
Negative Control ◽  
Ferric Reductase ◽  
Macrophage Differentiation ◽  
Iron Deficient ◽  
Significant Difference ◽  
Membrane Bound ◽  
Factor Assay

The enzyme ferric reductase catalyses the reduction of Fe(III) as a prerequisite to its transportation across the cell membrane. Duodenal mucosal biopsies from iron overloaded patients with genetic haemochromatosis (GH) have increased ferric reductase activity and iron absorption compared with controls, yet the GH mucosa is iron deficient. A similar GH-related iron deficiency is also seen in macrophages. The aim of this study was to investigate whether macrophage ferric reductase activity is altered in GH, and to determine ferric reductase activity in monocytes and differentiated macrophages. The erythroleukaemic K562 cell line was studied as a clonal reference cell line. The basal K562 ferric reductase activity is characteristic of a membrane bound enzyme, being both temperature and protease sensitive. Ferric reductase activity was also demonstrated in human leucocyte, monocyte and macrophage preparations. Assays of K562 and macrophage cell supernatants confirmed that the ferric reductase activity was not due to a secreted factor. Assay of ferric reductase in normalized-iron and iron-enriched (100 µM ferric citrate) conditions showed no significant difference between Cys282Tyr (Cys282 → Tyr) homozygous GH macrophages and Cys282-Tyr negative control activities (P> 0.05). However, a 900% increase in ferric reductase activity was observed during monocyte to macrophage differentiation (P< 0.05), possibly reflecting the co-ordinate up-regulation of iron metabolism in these cells. The demonstration of approx. 25% activity after macrophage differentiation at high free-iron concentrations compared with ‘normalized ’ iron is consistent with repression of human ferric reductase activity by iron. The identification of the human ferric reductase gene and its protein will ultimately provide insight into its regulation and role in mammalian iron metabolism.

scholarly journals Targeting BET Bromodomains in Pre-Clinical Models of Burkitt Lymphoma

Blood ◽  
2016 ◽  
Vol 128 (22) ◽  
pp. 5381-5381 ◽  
Author(s):  
Thomas Ippolito ◽  
Juan J Gu ◽  
Gregory Tang ◽  
Cory Mavis ◽  
Rodney R. Miles ◽  
...  
Keyword(s):  
Western Blot ◽  
Cell Viability ◽  
Cell Line ◽  
Cell Lines ◽  
Burkitt Lymphoma ◽  
Mtor Pathway ◽  
Synergistic Activity ◽  
Pi3k Activation ◽  
Induction Of Apoptosis

Abstract Background: Burkitt lymphoma (BL) is the most common NHL type in children. Although treatment for pediatric BL has improved significantly, there is an urgent need for novel therapies that reduce the toxicity of modern treatment regimens and improve on the dismal survival observed in the relapsed/refractory setting where only about 20-30% of patients survive their disease. Recent reports have implicated co-activation of c-Myc and PI3K in Burkitt lymphomagenesis. Genomic analysis of recurrent oncogenic mutations in BL have identified tonic B-cell receptor signaling and the over-expression of Myc induced microRNAs from the MIR17-92 family, e.g. mir17 and mir19, as possible mechanisms of PI3K activation in BL. Mir17 and mir19, have been implicated in Burkitt lymphomagenesis and their overexpression may be associated with a higher risk of relapse. The protein phosphatase and tensin homolog (PTEN) is a major regulator of PI3K pathway activation. MIR17-92 cluster members have been shown to target PTEN leading to increased PI3K activation. We have previously identified increased expression of mir17 and mir19 along with increased activation of AKT in cell line models of chemotherapy resistant BL suggesting a potential mechanism for increased resistance. BET bromodomains interact with chromatin and enhance transcriptional activation of numerous genes including c-Myc. Thus, BET bromodomains represent a promising target in BL. To investigate the effects of inhibition of c-Myc driven activation of the PI3K/AKT/mTOR pathway, we characterized the activity of the highly potent small molecule bromodomain inhibitor JQ1 in chemotherapy sensitive and resistant BL cell lines. Additionally, we analyzed the ability to enhance anti-lymphoma activity of PI3K/Akt/mTOR pathway inhibition in BL by the combination of BET bromodomain inhibition and targeted inhibition of the PI3K/AKT/mTOR pathway. Methods: The in vitro activity of JQ1 was investigated in the BL cell lines Raji, Raji 4RH (chemotherapy-rituximab resistant), Raji 8RH (rituximab resistant), Ramos, and Daudi. Cell Viability following exposure to JQ1 alone and in combination with the PI3K/mTOR inhibitor omipalisib (GSK458)) was analyzed using Cell-Titer Glo or Alamar Blue assays following 24, 48, and 72 hour exposure over a range of inhibitor concentrations. Induction of apoptosis was analyzed using western blotting for cleaved PARP. C-Myc expression following JQ1 exposure was determined by western blot following 48 hour JQ1 exposure. The effect of JQ1 exposure on the expression of c-Myc induced microRNA expression was determined by qRT-PCR in cells exposed to JQ1 for 48hours. Synergy of combination exposures was determined using CalcuSyn to generate combination index (CI) values. Results: In vitro exposure of BL cell lines to JQ1 for 24, 48, and 72 hours resulted in a significant dose and time dependent decrease in viable cells (72 hour IC50 values: Raji 0.12µM, Raji 4RH 1.7µM, Raji 8RH 0.7µM, Ramos 0.22µM and Daudi 4µM). There was an increase in cleaved PARP after 72 hour exposure indicating induction of apoptosis. While single agent effect was seen in the resistant Raji 4RH cell line, activity was noted to primarily occur at the higher end of the dosing. Western blot analysis demonstrated a reduction in c-Myc expression following exposure to JQ1 1µM for 24 hours (relative band intensity normalized to control: Raji=0.12, Raji 4RH=0.18, Raji 8RH=0.11). qPCR showed a reduction in Mir17 relative transcription levels after 48 hours of exposure to JQ1 2.5mM (relative expression compared to control: Raji=0.72, Raji 4RH=0.98, Raji 8RH=0.83, Ramos=0.57, Daudi=0.46). When combined with omipalisib, an increased effect on cell viability was noted. The combination effect was noted to be synergistic (CI<0.9) at multiple dose combinations while other combinations exhibited primarily additive effects. Conclusion: In vitro inhibition of BET bromodomains with JQ1 results in a decrease in c-Myc expression and a decrease in c-Myc dependent miR expression with impaired proliferation and induction of apoptosis in chemotherapy-sensitive and -resistant BL cell lines. Augmented, and in some cases synergistic, activity is also noted with dual inhibition of BET bromodomains and the PI3K/Akt/mTOR pathway in BL cell lines. Disclosures No relevant conflicts of interest to declare.

scholarly journals In vitro evaluation of the odontogenic potential of mouse undifferentiated pulp cells

2012 ◽  
Vol 23 (4) ◽  
pp. 328-336 ◽  
Author(s):  
Mayara Sgarbi Semeghini ◽  
Roger Rodrigo Fernandes ◽  
Daniela Thomazatti Chimello ◽  
Fabíola Singaretti de Oliveira ◽  
Karina Fittipaldi Bombonato-Prado
Keyword(s):  
Alkaline Phosphatase ◽  
Cell Viability ◽  
Cell Line ◽  
Real Time ◽  
Total Protein ◽  
Negative Control ◽  
Total Protein Content ◽  
Dental Tissue ◽  
Pulp Cells

The aim of this study was to evaluate the odontogenic potential of undifferentiated pulp cells (OD-21 cell line) through chemical stimuli in vitro. Cells were divided into uninduced cells (OD-21), induced cells (OD-21 cultured in supplemented medium/OD-21+OM) and odontoblast-like cells (MDPC-23 cell line). After 3, 7, 10 and 14 days of culture, it was evaluated: proliferation and cell viability, alkaline phosphatase activity, total protein content, mineralization, immunolocalization of dentin matrix acidic phosphoprotein 1 (DMP1), alkaline phosphatase (ALP) and osteopontin (OPN) and quantification of genes ALP, OSTERIX (Osx), DMP1 and runt-related transcription factor 2 (RUNX2) through real-time polymerase chain reaction (PCR). Data were analyzed by Kruskal-Wallis and Mann-Whitney U tests (p<0.05). There was a decrease in cell proliferation in OD-21 + OM, whereas cell viability was similar in all groups, except at 7 days. The amount of total protein was higher in group OD-21 + OM in all periods; the same occurred with ALP activity after 10 days when compared with OD-21, with no significant differences from the MDPC-23 group. Mineralization was higher in OD-21+OM when compared with the negative control. Immunolocalization demonstrated that DMP1 and ALP were highly expressed in MDPC-23 cells and OD-21 + OM cells, whereas OPN was high in all groups. Real-time PCR revealed that DMP1 and ALP expression was higher in MDPC-23 cell cultures, whereas RUNX2 was lower for these cells and higher for OD-21 negative control. Osx expression was lower for OD-21 + OM. These results suggest that OD-21 undifferentiated pulp cells have odontogenic potential and could be used in dental tissue engineering.

scholarly journals The effect of NPM1 silencing on the tumorigenesis of drug-resistant bladder cancer and related signaling pathway

2020 ◽  
Author(s):  
Chenshuo Luo ◽  
Ting Lei ◽  
Man Zhao ◽  
Qian Meng ◽  
Zhang Man
Keyword(s):  
Mass Spectrometry ◽  
Bladder Cancer ◽  
Cell Line ◽  
Signaling Pathway ◽  
Nude Mice ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Migration Ability ◽  
Significant Difference

Abstract Background: NPM1 can provide abundant information of bladder cancer changes, but the effect of NPM1 differential expression on tumor and tumor related molecular mechanism has not been elucidated.Methods: NPM1 silencing cell line was established by lentivirus. The tumorigenic ability was judged by wound-healing assay, transwell invasion assay and nude mice tumorigenicity assay. The proteome of NPM1 deficient bladder cancer cell line was analyzed by Liquid Chromatography Mass Spectrometry (LC-MS). The results of mass spectrometry are comprehensively analyzed by bioinformatics analysis for tumor related molecules. The signal pathways involved in tumor related molecules will be verified by KEGG and UniProt databases.Results: Compared with the corresponding negative control group, NPM1 silencing cell line T24/DDP Lv-NPM1 showed strong migration ability and high invasive ability. There was no significant difference in migration ability and the invasive cells proportion between NPM1 overexpressing cell line and related negative control group. The tumorigenesis in nude mice also showed that NPM1 silencing tumor had larger tumor volume. Among all differential proteins analyzed by mass spectrometry, 20 proteins with signal transduction activity showed the most significant difference (Fold change > 1.5). 6 of them were associated with NF-κB signaling pathway, which may play an important role in the development of tumor.Conclusions: The loss of NPM1 may indicate the poor outcome of bladder cancer. Abnormal expression of NF-κB signaling pathway is an important factor in the progression of bladder cancer. Monitoring NPM1 expression can effectively adjust the treatment strategy of bladder cancer.

scholarly journals Cytotoxicity and Elemental Release of Dental Acrylic Resin Modified with Silver and Vanadium Based Antimicrobial Nanomaterial

2021 ◽  
Vol 23 (1) ◽  
pp. 12-17
Author(s):  
Denise Tornavoi De Castro ◽  
Ana Beatriz Vilela Teixeira ◽  
Oswaldo Luiz Alves ◽  
Andréa Cândido dos Reis
Keyword(s):  
Cell Viability ◽  
Inductively Coupled Plasma ◽  
Acrylic Resin ◽  
Positive Control ◽  
Negative Control ◽  
Polymeric Film ◽  
Inductively Coupled ◽  
Significant Difference ◽  
Dental Acrylic ◽  
Plasma Mass Spectrometry

AbstractThe acrylic resin used for the prosthesis base accumulates biofilm, causing diseases such as stomatitis. The addition of some nanoparticles promotes antimicrobial action. This study incorporated the nanostructured silver vanadate decorated with silver nanoparticles (AgVO3) to the acrylic resin by two methods and evaluated the cytotoxicity for human gingival fibroblasts (HGF) and the released silver and vanadium ions. The concentrations of 0.5, 1, 2.5, and 5% of AgVO3 was incorporated by vacuum spatulation and polymeric film. The vacuum spatulation was performed for 60 s using the Turbomix equipment, and the polymeric film was obtained from the polymer solubilization in chloroform, the film was subjected to a cryogenic grinding, and the powder obtained was manually mixed at the monomer. HGF cell viability was assessed after 24 hours, 7 and 14 days by the MTT assay. The release of silver (Ag) and vanadium (V) ions were quantified by inductively coupled plasma mass spectrometry after 30 days. Kruskal-Wallis and Dunn’s test were applied (α = 0.05). The HGF viability was inversely proportional to the incubation time. Both incorporation techniques and the negative and positive control groups presented significant statistical differences (p<0.05). The experimental groups presented no statistical difference compared to the negative control (p>0.05), except the vacuum spatulation group with 5% of AgVO3 that showed greater viability than the negative control (p=0.013) in 24 hours. The release of Ag and V ions was proportional to the concentration of AgVO3 The 5% group presented a significant difference compared to the other groups (p<0.05). In conclusion, the acrylic resin with and without the AgVO3 incorporation had a small cytotoxic potential for HGF in 24 hours, with a lower viability in longer contact times; the release of Ag and V ions was proportional to the concentration of AgVO3, not influencing cell viability. Keywords: Acrylic Resins. Cell Survival. Nanotechnology. Ions. ResumoA resina acrílica utilizada para a base da prótese acumula biofilme, causando doenças como a estomatite. A adição de algumas nanopartículas promove ação antimicrobiana. Este estudo incorporou o vanadato de prata nanoestruturado decorado com nanopartículas de prata (AgVO3) à resina acrílica por dois métodos e avaliou a citotoxicidade para fibroblastos gengivais humanos (HGF) e os íons prata e vanádio liberados. As concentrações de 0,5%, 1%, 2,5% e 5% de AgVO3 foram incorporadas por espatulação a vácuo e filme polimérico. A espatulação a vácuo foi realizada por 60 s no equipamento Turbomix, e o filme polimérico foi obtido a partir da solubilização do polímero em clorofórmio, o filme foi submetido a uma moagem criogênica e o pó obtido foi misturado manualmente ao monômero. A viabilidade celular de HGF foi avaliada após 24 horas, 7 e 14 dias pelo ensaio de MTT. A liberação de íons prata (Ag) e vanádio (V) foi quantificada por espectrometria de massa com plasma indutivamente acoplado após 30 dias. Os testes de Kruskal-Wallis e Dunn foram aplicados (α=0,05). A viabilidade de HGF foi inversamente proporcional ao tempo de incubação. As técnicas de incorporação e os grupos controle negativo e positivo apresentaram diferença estatisticamente significante (p<0,05). Os grupos experimentais não apresentaram diferença estatística em relação ao controle negativo (p>0,05), exceto o grupo de espatulação a vácuo com 5% de AgVO3 que apresentou maior viabilidade que o controle negativo (p = 0,013) em 24 horas. A liberação de íons Ag e V foi proporcional à concentração de AgVO3. O grupo 5% apresentou diferença significativa em relação aos demais grupos (p <0,05). Em conclusão, a resina acrílica com e sem a incorporação de AgVO3 apresentou um pequeno potencial citotóxico para o HGF em 24 horas, com menor viabilidade nos tempos de maior contato, e a liberação de íons Ag e V foi proporcional à concentração de AgVO3, não influenciando na viabilidade celular. Palavras-chave: Resinas Acrílicas. Sobrevivência Celular. Nanotecnologia. Íons.

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