Reflection-Mode Ultrasound Computed Tomography Based on Wavelet Processing for High-Contrast Anatomical and Morphometric Imaging

Medical B-mode ultrasound is widely used for the examination of children’s limbs, including soft tissue, muscle, and bone. However, for the accurate examination of the bone only, it is often replaced by more restrictive clinical modalities. Several authors have investigated ultrasonic imaging of bone to assess cortical thickness and/or to estimate the wave velocity through the internal structure. The present work focuses on the transverse slice imaging process using reflection-mode ultrasound computed tomography (USCT). The method is valid for imaging soft tissues with similar acoustic impedances, but in the presence of bone, the higher contrasts alter the propagation of ultrasonic waves and reduce the contrast-to-noise ratio (CNR). There is a need to change the methods used for the processing of ultrasonic signals. Our group has developed a wavelet-based coded excitation (WCE) method to process information in frequency and time. The objective of this study is to use the method to improve reflection-mode USCT, at low ultrasonic intensities, to better address organ morphometry. Experimental results on a newborn arm phantom and on an ex vivo chicken drumstick are presented, and the usefulness of this WCE-mode USCT is discussed.

Quantification of visceral adipose tissue in polycystic ovary syndrome: dual-energy X-ray absorptiometry versus magnetic resonance imaging

Background Polycystic ovary syndrome (PCOS) is associated with frequent overweight and abdominal obesity. Quantifying visceral adipose tissue (VAT) in PCOS patients can be a tool to assess metabolic risk and monitor effects of treatment. The latest dual-energy X-ray absorptiometry (DXA) technology can measure VAT and subcutaneous adipose tissue (SAT) in a clinical setting. Purpose To compare DXA-measurements of VAT and SAT with the gold standard MRI in women with PCOS. Material and Methods A cross-sectional study of 67 overweight women with PCOS was performed. Measurements of VAT and SAT were performed by DXA in a 5-cm thick transverse slice at the L4/L5 level and by MRI in a 1-cm thick transverse slice at the L3 level. Results Mean (SD) DXA-VAT was 81 (34) cm3, DXA-SAT was 498 (118) cm3, MRI-VAT was 117 (48) cm3, and MRI-SAT was 408 (122) cm3. MRI and DXA measures of VAT (r = 0.82, P < 0.001) and SAT (r = 0.92, P < 0.001) correlated closely, and DXA-VAT was stronger correlated with MRI-VAT than BMI (r = 0.62, P < 0.001) and waist circumference (r = 0.60, P < 0.001). DXA-VAT coefficient of variance was 6.7% and inter correlation coefficient was 0.98. Bland–Altman analyses showed DXA to slightly underestimate VAT and SAT measurements compared with MRI. Conclusion DXA and MRI measurements of VAT and SAT correlated closely despite different size of region of interest, and DXA-VAT was superior to waist circumference and BMI in estimating MRI-VAT. DXA showed high reproducibility making it is suitable for repeated measurements in the same individual over time.

Three-Dimensional Tooth Segmentation by Integrating Multiple Ray-Sum Images From CBCT Data

The reconstruction of tooth anatomies is of utmost importance when dental implant surgeries and/or orthodontic corrections must be planned. In the last few years, cone beam CT (CBCT) has gained popularity in dentistry for 3D imaging of jawbones and teeth. However, within CBCT data sets, each tooth is defined by a region, which cannot be easily separated from surrounding tissues (i.e., bone tissue) by only considering pixel’s grey-intensity values. For this reason, some enhancement is usually necessary in order to properly segment tooth geometries. In this paper, a semi-automatic approach to reconstruct individual 3D tooth anatomies by processing CBCT-scan data is presented. The methodology is based on the creation of a minimal number of 2D “local ray-sum” images by adding the absorption values of adjacent voxels along the most significant views for each tooth. The knowledge of the specific anatomical patient morphology drives the selection of these significant projection directions. The reconstructed “ray-sum” images greatly enhance the clearness of the root contours, which can then be interactively traced by dentists. A set of meaningful 2D tooth contours is consequently obtained and used to automatically extract a cubic spline curve for each transverse slice, thus approximating the overall 3D tooth profile. The effectiveness of the methodology has been evaluated by comparing the results obtained for the reconstruction of anterior teeth with those obtained by using classical segmentation tools provided within commercial software.

Unfolding plane curves with cusps and nodes

Given an irreducible surface germ (X, 0) ⊂ (ℂ3, 0) with a one-dimensional singular set Σ, we denote by δ1 (X, 0) the delta invariant of a transverse slice. We show that δ1 (X, 0) ≥ m0 (Σ, 0), with equality if and only if (X, 0) admits a corank 1 parametrization f :(ℂ2, 0) → (ℂ3, 0) whose only singularities outside the origin are transverse double points and semi-cubic cuspidal edges. We then use the local Euler obstruction Eu(X, 0) in order to characterize those surfaces that have finite codimension with respect to -equivalence or as a frontal-type singularity.

Centroid and envelope dynamics of charged particle beams in an oscillating wobbler and external focusing lattice for heavy ion fusion applications

Recent heavy ion fusion target studies show that it is possible to achieve ignition with direct drive and energy gain larger than 100 at 1 MJ. To realize these advanced, high-gain schemes based on direct drive, it is necessary to develop a reliable beam smoothing technique to mitigate instabilities and facilitate uniform deposition on the target. The dynamics of the beam centroid can be explored as a possible beam smoothing technique to achieve a uniform illumination over a suitably chosen region of the target. The basic idea of this technique is to induce an oscillatory motion of the centroid for each transverse slice of the beam in such a way that the centroids of different slices strike different locations on the target. The centroid dynamics is controlled by a set of biased electrical plates called “wobblers.” Using a model based on moments of the Vlasov-Maxwell equations, we show that the wobbler deflection force acts only on the centroid motion, and that the envelope dynamics are independent of the wobbler fields. If the conducting wall is far away from the beam, then the envelope dynamics and centroid dynamics are completely decoupled. This is a preferred situation for the beam wobbling technique, because the wobbler system can be designed to generate the desired centroid motion on the target without considering its effects on the envelope and emittance. A conceptual design of the wobbler system for a heavy ion fusion driver is briefly summarized.

Glycine Binding Site of the Synaptic NMDA Receptor in Subpostremal NTS Neurons

The nucleus of the tractus solitarius (NTS) plays an important role in the control of several autonomic reflex functions and has glutamate and GABA as main neurotransmitters. In this work, we used patch-clamp recordings in transverse slice preparations from rats to study whether the glycine binding site of the N-methyl-d-aspartate (NMDA) receptor is saturated or not in neurons of the subpostremal NTS. Except at hyperpolarized voltages and close to the reversal potential, glycine potentiated the NMDA responses in a concentration-dependent manner. The total charge transferred by glutamatergic currents was enhanced by glycine (500 μM; from 28 ± 13 to 42 ± 18 pC at +50 mV, n = 7, P < 0.05). Glycine increased the conductance of the postsynaptic membrane, without altering its reversal potential, both in the presence (from 2.4 ± 0.06 to 3.4 ± 0.09 nS; n = 7) and absence (from 3.1 ± 0.06 to 4.4 ± 0.10 nS; n = 8) of Mg2+ in the bathing solution. d-serine, in the presence of strychnine, also increased the amplitude of the NMDA component (by 68 ± 19%, P < 0.05, n = 5). The membrane potential was hyperpolarized (16 ± 6 mV, n = 8) by glycine, suggesting the presence of inhibitory glycinergic receptors. Our results indicate that the glycine site of the NMDA receptor in neurons of the subpostremal NTS is not saturated and that glycine may act as a modulator of the NMDA transmission in this nucleus.