Geochemical characteristics and genetic families of crude oil in DWQ oilfield, Kuqa Depression, NW China

As one of the most petroliferous oil producing area in Kuqa depression, Dawanqi (DWQ) oilfield is supplying with great attention. In this regard, the geochemical characteristics and oil families from DWQ field were investigated using molecular compounds analysis of GC, GC–MS techniques. The bulk geochemistry of oils from DWQ oilfield displays complicated molecular composition characteristics, including relative higher indices of Pr/Ph (1.4 ~ 4.26, with an average of 2.4), high concentration of light hydrocarbons and certain abundant pentacyclic triterpene and steranes. The C7 light hydrocarbon and isoprenoids ratios indicate the oils were derived from terrestrial and higher plant input in weak oxidizing and reducing environment. Most of the oils are among the mature oils in the study area, except a few samples that are identified as slightly biodegraded by C7 hydrocarbon. Three oil families are identified in DWQ oilfield of Kuqa depression by biomarker analysis and geochemical parameters. The family A shares the attributes with higher amount of tricyclic terpanes, such as C19- C20 tricyclic terpane, higher C24-tercyclic terpane, lower concentration of gammacerane (< 0.6) but poor diasteranes. Family C is characterized with lower content of C19-tricyclic terpane than C20 tricyclic terpane, low C24-tercyclic terpane than C23-tricycli terpane, relative high concentration of gammacerane (> 0.6) but poor diasteranes. The oils of family B are mixed from the two types, showing mixed features of family A and C. The results can shed light for the exploration of the studied area.

Investigation on the biodegradation levels of super heavy oils by parameter-striping method and refined Manco scale: a case study from the Chepaizi Uplift of Junggar Basin

The Carboniferous volcanic reservoir in the Chepaizi Uplift became an exploration hot target in recent years for its substantial amount of oils discovered. However, most of the Carboniferous heavy oils were biodegraded to PM7 or higher with orders of magnitude variation in oil viscosities. Two oil groups (I and II) exactly corresponding to the western and eastern Chepaizi Uplift were distinguished according to their source diagnose. Furthermore, three oil families (II1, II2 and II3), with the biodegradation level of PM7, PM8–8+, PM9+, respectively, were classified based on molecular compositions and parameter-stripping method of strongly bioresistant parameters. Allowing for this extremely high biodegradation case, more biodegradation refractory compound class were added to establish a refined Manco scale to quantitatively evaluate the biodegradation extent. Refined Manco number (RMN2) positively correlated with the oil density, NSO contents, and absolute concentrations of diasteranes and gammacerane, negatively correlated with the absolute concentrations of diahopane, summed tricyclic terpanes and pentacyclic terpanes. This refined scale showed higher resolution than the PM one to differentiate the biodegradation extent of Carboniferous heavy oils from the Chepaizi Uplift, especially those with same PM values but different oil viscosities.

Organic geochemical characteristics and comparison of the source rocks and the severely biodegradable oil sands around Karamay, China

For the sake of explaining the organic geochemical characteristics of the hydrocarbon source rocks (SRs) around Karamay, such as thermal maturity (TM) (evidences from the temperature of maximum pyrolysis yield (Tmax), the Carbon Preference Index (CPI) and Odd to Even Preference (OEP) values, the C30 M/H versus Tm/Ts, the C29 ββ/(ββ + αα) versus C29 20 S/(20 S + 20 R)), hydrocarbon potential (HP) (evidences from total organic carbon (TOC) and rock pyrolysis (RP)), organic matter (OM) source input (evidences from n-Alkane distribution, the relative quantity of C27-C28-C29 steranes, the (C19 + C20)/C23 tricyclic terpane (TT), and the Terrigenous/aquatic ratio (TAR)) as well as paleoenvironment (evidences from the pristane/phytane (Pr/Ph) and the Gammacerane index), and in order to analyze the biodegradation degree and source of oil sand (OS) oil, 55 core samples of SRs in all were selected to measure TOC and RP. 14 core samples of SRs and 3 OS samples were selected for gas chromatography–mass spectrometry (GC-MS) analysis. 11 core samples of SRs and 3 OS samples were used for organic carbon isotope (OCI) analysis. The results indicate that except the SR from Fengcheng formation (P1 f) which is in the stage of high or over evolution, the other SRs are in mature stage. OM type of Carboniferous (C) SRs are type Ⅱ kerogen, with the largest abundance. OM type of the SRs in Wuerhe formation (P2 w) and Jiamuhe formation (P1 j) are type Ⅲ kerogen with good and medium OM abundances. Almost all SRs were formed in the reduction environment of high salinity, and the main OM source input is the lower aquatic plants. Karamay formation (T2 k) and Badaowan formation (J1 b) oil sands (OSs) around Karamay have undergone serious biodegradation. It is suggested that OS oil are mainly from P1 f SRs (evidences from the tricyclic terpanes (TT), triarysteranes, pregnane, homopregnane and OCI).

C1 compounds shape the microbial community of an abandoned century-old oil exploration well

The search for microorganisms that degrade hydrocarbons is highly relevant because it enables the bioremediation of these substances cheaply and without dangerous by-products. In this work, we studied the microbial communities of an exploratory oil well, abandoned a century ago, located in the Cahuita National Park of Costa Rica. Cahuita well is characterized by a continuous efflux of methane and the presence of a mixture of hydrocarbons including C2-dibenzothiophene, phenanthrene or anthracene, fluoranthene pyrene, dibenzothiophene, tricyclic terpanes, pyrene, sesquiterpenes, sterane and n-alkanes. Based on the analysis of 16S rRNA gene amplicons, we detected a significant abundance of methylotrophic bacteria (Methylobacillus (6.3-26.0 % of total reads) and Methylococcus (4.1-30.6 %)) and the presence of common genera associated with hydrocarbon degradation, such as Comamonas (0.8-4.6 %), Hydrogenophaga (1.5-3.3 %) Rhodobacter (1.0-4.9 %) and Flavobacterium (1.1-6.5 %). We evidenced the presence of methane monooxygenase (MMO) activities, responsible for the first step in methane metabolism, by amplifying the pmo gene from environmental DNA. We also isolated a strain of Methylorubrum rhodesianum, which was capable of using methanol as its sole carbon source. This work represents a contribution to the understanding of the ecology of communities of microorganisms in environments with permanently high concentrations of methane and hydrocarbons, which also has biotechnological implications for the bioremediation of highly polluting petroleum components.

Biomarker distributions and depositional environments of continental source rocks in Sichuan Basin, SW China

In order to study the distributions of the biomarker of the continental source rocks in the Sichuan Basin, 71 source rock samples were collected from the Upper Triassic-Lower Jurassic strata in different regions. The n-alkanes, isoprenoids, terpane, sterane, sesquiterpenes, caranes and aromatics in the extracts were analyzed in detail. GC-MS analysis has been conducted to analyze the biomarker of the continental source rocks. The results of GC-MS analysis indicate that the Upper Triassic source rocks are high in the content of extended tricyclic terpanes, pristane, phytane, gammacerane, C28 regular sterane and carotene. However, they are low in content of rearranged compounds. The ratio of Pr/Ph is less than 1, with the characteristics of tricyclic terpane C21 > C23. The Lower Jurassic source rocks are extremely low in content (even zero) of extended tricyclic terpanes, pristane, phytane, gammacerane, C28 regular sterane and carotene, and high in content of rearranged compounds. The ratio of Pr/Ph is more than 1, with tricyclic terpane C21 > C23. These characteristics are still preserved after maturation. Moreover, during the sedimentation of the source rocks of T3x2–T3x3 members, the supply of continental plants was low (TAR < 1, with regular sterane C27 > C29, 1-MP/9-MP < 1). The source rocks of T3x5 member were low in salinity (slightly low content of gammacerane and carotene), being different significantly from the other Upper Triassic source rocks. In addition, during the sedimentation of the source rocks of J1dn Member, the supply of continental plants was also low (regular sterane C27 > C29, 1-MP/9-MP < 1), being quite different from that of J1l member. Through analysis of the difference in biomarkers, it is indicated that the sedimentary environment had changed from anoxic and brackish water during the Late Triassic to oxygen-rich and freshwater during the Early Jurassic in the Sichuan Basin. During this process, the types of organic matters had changed for several times.

BIOMARKER SIGNATURES IN TRIASSIC COPROLITES

ABSTRACT Coprolites are traditionally analyzed from a morphological perspective. Few studies exist from an organic geochemical perspective, and most of these consider recent specimens. This study represents an analysis of coprolites from deep time, using both traditional one-dimensional and also two-dimensional gas chromatography-mass spectrometry. We find organic molecules preserved in coprolites from the Triassic, and that both dietary habits of the defecators and paleoenvironment can be interpreted using comparative distributions of biomarker abundances in the coprolites. Steranes having 27 carbon atoms are known to be derived from animal steroids whereas those with 29 carbon atoms are known to be derived from plant steroids. The predominance of steranes with 27 carbon atoms over those with 29 carbon atoms in a non-marine environment was interpreted as evidence for the defecator(s) being predominantly carnivorous or possibly omnivorous. A series of tricyclic terpanes ranging from C19 to C28 was examined to determine the environment. The present study suggests that one or possibly all of the defecators may have been small-medium carnivores that lived in an aquatic or near aquatic setting.