This dissertation explores the images in the three treatises on the smallpox vaccine, which were illustrated between 1798 and 1850 in Hungary, which are János Stand’s, Sámuel Váradi’s and Mihály Kováts’s publications. It looks at the background of their authors and the production of their images, as well as the quality of the illustrations. As all three treatises have one illustration and they all follow the same visual strategy, the dissertation also seeks to describe and identify the scopes of such images, and to define their role in the implementation of the practice of vaccination in the wider public. Furthermore, the wider context of the images is also outlined, which aims at demonstrating what the illustrations can tell about contemporary medical culture and publishing in Hungary, the cultural dominance of Vienna within the Empire, and the transmission of knowledge, images, and cowpox matter in Europe and the Habsburg territories.