Application of UPLC/TOF-MS for Residue Analysis of Veterinary Drugs and Growth-Promoting Agents in Products of Animal Origin

The veterinary drugs are broad-spectrum antibacterial antibiotics; it uses to cure the animal disease. Many countries have banned veterinary drug residues like nitrofurans metabolites, chloramphenicol. However, the people were administrated veterinary drugs to animals as illegal to increase the milk production in animals for economic benefit. The results of illegally use of veterinary drugs remain as a residue in animal product like milk and it is very harmful to whom consume it cause cancer and allergic for human being which has entered the concern among milk consumers. To control illegal use of veterinary drugs, the government of India has restricted its use in animals. For the identification and confirmation of veterinary drug residues in animal products, analytical techniques such as liquid chromatography and mass spectrometry are available. These are very sophisticated equipments which are available nowadays and their methodologies for the analytical method validation are described by European commission 2002/657/EC. The use of veterinary drugs is a big challenge to effectively identify and authorization of their use. There are so many analytical techniques are using very effectively and taking very less time to protect the consumers from their adverse effects. These techniques take very less time to identify more groups of compounds such as tetracycline, sulfonamides, anthelmintic, and macrolides in single multi-residue method. These methods having validation parameters include system precision, calibration curve, accuracy, limit of detection, and quantification. Therefore, improvement in the existing technologies and accessibility of new screening methodologies will give opportunities for automation that helps in obtaining the results in very less time and improved sensitivity and specificity which contribute to better safety assurance, standard, and quality of various food products of animal origin.

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Identification of Species and Subspecies of Lactic Acid Bacteria Present in Spanish Cheeses Type “Torta” by MALDI-TOF MS and pheS gene Analyses

Microorganisms ◽

10.3390/microorganisms8020301 ◽

2020 ◽

Vol 8 (2) ◽

pp. 301

Author(s):

Fernando Sánchez-Juanes ◽

Vanessa Teixeira-Martín ◽

José Manuel González-Buitrago ◽

Encarna Velázquez ◽

José David Flores-Félix

Keyword(s):

Lactic Acid ◽

Lactic Acid Bacteria ◽

Lactobacillus Rhamnosus ◽

Taxonomic Status ◽

Raw Milk ◽

Animal Origin ◽

Lactobacillus Curvatus ◽

Maldi Tof Ms ◽

Maldi Tof ◽

Tof Ms

Several artisanal cheeses are elaborated in European countries, being commonly curdled with rennets of animal origin. However, in some Spanish regions some cheeses of type “Torta” are elaborated using Cynara cardunculus L. rennets. Two of these cheeses, “Torta del Casar” and “Torta de Trujillo”, are elaborated in Cáceres province with ewe’s raw milk and matured over at least 60 days without starters. In this work, we identified the lactic acid bacteria present in these cheeses using MALDI-TOF MS and pheS gene analyses, which showed they belong to the species Lactobacillus curvatus, Lactobacillus diolivorans, Lactobacillus paracasei, Lactobacillus plantarum, Lactobacillus rhamnosus, Lactococcus lactis and Leuconostoc mesenteroides. The pheS gene analysis also allowed the identification of the subspecies La. plantarum subsp. plantarum, La. paracasei subsp. paracasei and Le. mesenteroides subsp. jonggajibkimchii. Low similarity values were found in this gene for some currently accepted subspecies of Lc. lactis and for the two subspecies of La. plantarum, and values near to 100% for the subspecies of Le. mesenteroides and La. paracasei. These results, which were confirmed by the calculated ANIb and dDDH values of their whole genomes, showed the need to revise the taxonomic status of these species and their subspecies.

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Multi-residue analysis of pesticides, plant hormones, veterinary drugs and mycotoxins using HILIC chromatography – MS/MS in various food matrices

Analytica Chimica Acta ◽

10.1016/j.aca.2016.09.011 ◽

2016 ◽

Vol 942 ◽

pp. 121-138 ◽

Cited By ~ 47

Author(s):

G.P. Danezis ◽

C.J. Anagnostopoulos ◽

K. Liapis ◽

M.A. Koupparis

Keyword(s):

Plant Hormones ◽

Residue Analysis ◽

Veterinary Drugs ◽

Food Matrices

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Development of a simultaneous multi-residue analysis for screening and confirmation of 7 veterinary drugs in bovine milk by LC-MSMS

Journal of Preventive Veterinary Medicine ◽

10.13041/jpvm.2019.43.2.68 ◽

2019 ◽

Vol 43 (2) ◽

pp. 68-73

Author(s):

MinKyoung Kim ◽

◽

NamSun Kim ◽

HyunJung Kwon ◽

SungYong Ha ◽

...

Keyword(s):

Residue Analysis ◽

Bovine Milk ◽

Veterinary Drugs

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Quantitative Multiclass Pesticide Residue Analysis in Apple, Pear, and Grape by Modified QuEChERS and Liquid Chromatography Coupled to High-Resolution Mass Spectrometry

Journal of AOAC International ◽

10.5740/jaoacint.16-0276 ◽

2016 ◽

Vol 99 (6) ◽

pp. 1426-1435 ◽

Cited By ~ 5

Author(s):

Juliana S Munaretto ◽

Mariela de S Viera ◽

Manoel L Martins ◽

Martha B Adaime ◽

Renato Zanella

Keyword(s):

High Resolution ◽

Pesticide Residue ◽

High Resolution Mass Spectrometry ◽

Residue Analysis ◽

Mass Accuracy ◽

Reliable Technique ◽

Average Mass ◽

Modified Quechers ◽

Tof Ms

Abstract Most of the analytical methods currently applied in food control laboratories are focused on the determination of target compounds using LC coupled to tandem MS, which is an effective technique, but low-resolution MS is limited. Thus, a method for determination of pesticide multiresidues in fruits (pear, apple, and grape) using a modified quick, easy, cheap, effective, rugged, and safe method and LC coupled to quadrupole time-of-flight (Q-TOF) MS was developed and validated. The proposed method showed good linearity (r2 > 0.99) from 1 to 100 μg/L. Recoveries for blank samples spiked at 0.01, 0.04, and 0.10 mg/kg were between 66 and 122%, with RSDs <28%. Respective LOQs for apple, pear, and grape matrixes were 0.01 mg/kg for 112, 120, and 118 compounds, and 0.04 mg/kg for 22, 12, and 17 compounds, and average mass accuracy error was 3.2 ppm. LC with Q-TOF MS detection using protonated molecular ion and/or adducts and mass accuracy provided reliability for the method. The proposed method is effective for pesticide residue determination in apple, pear, and grape samples, proving that high-resolution MS using full scan mode can be a powerful and reliable technique for quantification purposes, being adequate for application in the surveillance of maximum residue limits set by different legislations.

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Deteksi Kejadian Residu Tetrasiklin pada Daging Ikan Nila di Kota Yogyakarta dengan Kromatografi Cair Kinerja Tinggi (KCKT)

Jurnal Sain Veteriner ◽

10.22146/jsv.34463 ◽

2019 ◽

Vol 37 (2) ◽

pp. 185

Author(s):

Wari Pawestri ◽

Gagak Dhony Satria ◽

Nisa Hakimah ◽

Doddi Yudhabuntara

Keyword(s):

High Performance ◽

Residue Analysis ◽

Animal Origin ◽

Chemical Contamination ◽

Traditional Markets ◽

Food Of Animal Origin ◽

Absolute Requirement ◽

Antibiotics Residues ◽

Meat Samples ◽

Tetracycline Residues

Food products of animal origin which are free from biological and chemical contamination are an absolute requirement of food safety. Antibiotics residues in the food of animal origin is one of the chemical contaminants in food are harmful/hazardous to human health. Tetracycline is an antibiotic that is often used in the fishing industry. The study aims to detect the occurrence of tetracycline residues in tilapia sold in traditional markets in Kota Yogyakarta. The research was conducted with detection test (detect disease). The study used 61 samples of tilapia fish from 16 traditional markets in Kota Yogyakarta. Tilapia meat samples are prepared according to the Association of Official Analytical Chemistry (AOAC). The existence of tetracycline residues in meat can be detected by high performance liquid chromatography (HPLC). Residue analysis in fish meat was conducted at the Department of Pharmacology, Faculty of Veterinary Medicine of Gadjah Mada University using HPLC Shimadzu version 6.1. The results showed that 24 samples of tilapia meat marketed in Kota Yogyakarta was positively contained tetracycline antibiotic residues. A total of 19 samples contained residues above maximum residue limits (MRL). The conclusion of the study is 31% of tilapia marketed in Kota Yogyakarta contains tetracycline residues above MRL. Surveillance of residues in food of animal origin and further research in terms of reducing antibiotic residue in tilapia meat is highly recommended to improve the quality and safety of food of animal origin.

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Different perspectives associated with the development of relevant strategies for maintenance of withdrawal period of veterinary drugs: Bangladesh perspectives

Asian-Australasian Journal of Food Safety and Security ◽

10.3329/aajfss.v2i2.55910 ◽

2018 ◽

Vol 2 (2) ◽

pp. 71-78

Author(s):

Md Zobidul Kabir ◽

Alam Khan ◽

Md Jahanur Rahman ◽

Moizur Rahman

Keyword(s):

Monitoring System ◽

Quantitative Methods ◽

Public Awareness ◽

Veterinary Drugs ◽

Bank Loan ◽

Health Concern ◽

Animal Origin ◽

Withdrawal Period ◽

Capacity Enhancement ◽

Substantial Role

Veterinary drugs have proven to be one of the most valuable tools in preserving animal health. With an increase in the use and availability of veterinary drugs, residues of veterinary drugs in animal origin food has become a public health concern and has received much attention from government agencies, consumer level, media and other concerned public interest groups. The objectives of the study were to identify the perspectives associated with the poor management for maintenance of withdrawal period of veterinary drugs in Bangladesh so that the development of relevant strategies for maintenance of withdrawal period of veterinary drugs could be achieved in context of environmental realities in Bangladesh. The survey was interpretive in nature using quantitative methods. A non-experimental and descriptive research design was used to conduct this study. The survey study was carried out at seven divisions (i.e. Rangpur, Rajshahi, Khulna, Dhaka, Sylhet, Barishal and Chattogram) in Bangladesh during July 2012 to June 2018 by using close ended questionnaires among randomly selected respondents. Survey instruments were also designed to capture the perspectives of two different groups of government veterinarians: Group 1– Veterinary Surgeons of DLS, Bangladesh and Group 2 –Upazila Livestock Officers of DLS, Bangladesh. The findings indicated the knowledge, beliefs and perceptions of respondents. The research findings divulge that different perspectives/dimensions associated with economic loss, compensation support, awareness, ethical motivation, monitoring system, proper legislation and guideline, cooperative and group livestock production as well as marketing system had „moderate to substantial role‟ with the management for maintenance of withdrawal period of veterinary drugs in Bangladesh. Besides these, findings also indicated that other perspectives/dimensions associated with allocation of subsidies and Bank loan with low interest, opportunity of livestock/ poultry insurance services, capacity enhancement for monitoring system, availability of technology to producers level, extension work with motivation, magistracy empowerment for veterinarians, legal influence, capacity enhancement of DLS staff, building of public awareness on drug residue problem and availability of effective equipment as well as modern analytical techniques for diagnostic analysis of chemical/drug residues also had „moderate to substantial role‟ with the development of relevant strategies for maintenance of withdrawal period of veterinary drugs. Findings indicated that the two groups of veterinarians possessed near to similar/same perceptions across different dimensions/perspectives associated with the development of relevant strategies for maintenance of withdrawal period of veterinary drugs in Bangladesh and that no significant differences in perspectives existed (p> .05). Findings of this study could be implemented for formulation of effective policy as well as guideline with the development of relevant strategies for maintenance of withdrawal period of veterinary drugs in context of environmental realities in Bangladesh. Asian Australas. J. Food Saf. Secur. 2018, 2(2), 71-78

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