scholarly journals Deteksi Kejadian Residu Tetrasiklin pada Daging Ikan Nila di Kota Yogyakarta dengan Kromatografi Cair Kinerja Tinggi (KCKT)

2019 ◽  
Vol 37 (2) ◽  
pp. 185
Author(s):  
Wari Pawestri ◽  
Gagak Dhony Satria ◽  
Nisa Hakimah ◽  
Doddi Yudhabuntara
Keyword(s):  
High Performance ◽  
Residue Analysis ◽  
Animal Origin ◽  
Chemical Contamination ◽  
Traditional Markets ◽  
Food Of Animal Origin ◽  
Absolute Requirement ◽  
Antibiotics Residues ◽  
Meat Samples ◽  
Tetracycline Residues

Food products of animal origin which are free from biological and chemical contamination are an absolute requirement of food safety. Antibiotics residues in the food of animal origin is one of the chemical contaminants in food are harmful/hazardous to human health. Tetracycline is an antibiotic that is often used in the fishing industry. The study aims to detect the occurrence of tetracycline residues in tilapia sold in traditional markets in Kota Yogyakarta. The research was conducted with detection test (detect disease). The study used 61 samples of tilapia fish from 16 traditional markets in Kota Yogyakarta. Tilapia meat samples are prepared according to the Association of Official Analytical Chemistry (AOAC). The existence of tetracycline residues in meat can be detected by high performance liquid chromatography (HPLC). Residue analysis in fish meat was conducted at the Department of Pharmacology, Faculty of Veterinary Medicine of Gadjah Mada University using HPLC Shimadzu version 6.1. The results showed that 24 samples of tilapia meat marketed in Kota Yogyakarta was positively contained tetracycline antibiotic residues. A total of 19 samples contained residues above maximum residue limits (MRL). The conclusion of the study is 31% of tilapia marketed in Kota Yogyakarta contains tetracycline residues above MRL. Surveillance of residues in food of animal origin and further research in terms of reducing antibiotic residue in tilapia meat is highly recommended to improve the quality and safety of food of animal origin.

scholarly journals Detection of Antibiotic Residues in Chicken Meat and Eggs from Traditional Markets at Yogyakarta City Using Bioassay Method

2020 ◽  
pp. 1-6
Author(s):  
Dyah Ayu Widiasih ◽  
Yatri Drastini ◽  
Doddi Yudhabuntara ◽  
F. Lintang R. Daru Maya ◽  
Prisha Lini Sivalingham ◽  
...  
Keyword(s):  
Chicken Meat ◽  
Animal Origin ◽  
Antibiotic Residues ◽  
Traditional Markets ◽  
Bioassay Method ◽  
Food Of Animal Origin ◽  
Meat Samples ◽  
Tetracycline Residues ◽  
Meat And Eggs ◽  
Yogyakarta City

Studies on antibiotic residues content in food of animal origin are currently needed to support veterinary public health programs. The present study was described bioassay method for the detection of antibiotic residues in chicken meat and eggs from traditional market at Yogyakarta City. A number of twenty-four chicken meat samples and 24 egg samples were taken from 8 traditional markets in Yogyakarta city. Samples were examined at Centre for Veterinary Wates, Yogyakarta, Indonesia using bioassay method for screening detection of penicillin, aminoglycoside, macrolide and tetracycline residues. This bioassay method using some bacteria, such as Bacillus stearothermophillus, B. cereus, B. subtilis, and Kocuria rizophila. A percentage of the results showed that 8.33% (2/24) samples of chickens tested positively contained the oxytetracycline antibiotic residues. Meanwhile, as much as 75% (18/24) samples of positive eggs contain penicillin antibiotic residues, positive residues of aminoglycoside amounted to 12.5% (3/24) and the positive residues of oxytetracycline also amounted to 12.5% (3/24).

scholarly journals Undeclared (Poly)phosphates Detection in Food of Animal Origin as a Potential Tool toward Fraud Prevention

Foods ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1547
Author(s):  
Radmila Pavlovic ◽  
Federica Di Cesare ◽  
Francesca Longo ◽  
Franco Abballe ◽  
Sara Panseri ◽  
...  
Keyword(s):  
High Performance ◽  
High Resolution Mass Spectrometry ◽  
Exchange Chromatography ◽  
Animal Origin ◽  
Food Of Animal Origin ◽  
Conductometric Detection ◽  
Q Exactive ◽  
Phosphate Treatment ◽  
Potential Tool ◽  
Vulnerable Population Groups

(Poly)phosphates are approved as water-preserving and emulsifying agents that improve the appearance and consistency of many food products. The labelling of added (poly)phosphates is essential for protecting vulnerable population groups and to prevent unfair trade practices resulting in economic fraud. The problems with (poly)phosphates’ utilisation concerns both analytical and legislative issues, such as: (1) their straightforward detection; (2) excessive addition altering freshness perception and misleading consumers; (3) uncontrolled usage increasing foodstuff weight; (4) application in products where they are not permitted; and (5) no indication on the label. Bearing all these issues in mind, the main purpose of this study was the quantification and screening of the (poly)phosphates profile in meat, marine and dairy products (160 samples), of which 43 were without declared (poly)phosphate treatment. Analysis was completed by high-performance ion-exchange chromatography either with conductometric detection or coupled to Q-Exactive Orbitrap high-resolution mass spectrometry. Although the (poly)phosphates profiles varied greatly according to species and processing type, the following criteria for detection of illicit treatment were established: high orthophosphate level, quantified short-chain (poly)phosphate anions and the presence of long-chain forms. In conclusion, the instrumental platforms used in this study can be recommended to inspection bodies as reliable methods for the detection of food adulteration with (poly)phosphates.

scholarly journals Citrobacter braakii Yield False-Positive Identification as Salmonella, a Note of Caution

Foods ◽  
2021 ◽  
Vol 10 (9) ◽  
pp. 2177
Author(s):  
Joanna Pławińska-Czarnak ◽  
Karolina Wódz ◽  
Magdalena Kizerwetter-Świda ◽  
Tomasz Nowak ◽  
Janusz Bogdan ◽  
...  
Keyword(s):  
False Positive ◽  
Salmonella Enterica ◽  
Meat Products ◽  
Animal Origin ◽  
Biochemical Tests ◽  
Salmonella Spp ◽  
Isolation And Identification ◽  
False Positive Identification ◽  
Food Of Animal Origin ◽  
Meat Samples

Background: Globally, Salmonella enterica is one of the leading causes of foodborne illness in humans. Food of animal origin is obligatorily tested for the presence of this pathogen. Unfortunately, in meat and meat products, this is often hampered by the presence of background microbiota, which may present as false-positive Salmonella. Methods: For the identification of Salmonella spp. from meat samples of beef, pork, and poultry, the authorized detection method is PN-EN ISO 6579-1:2017-04 with the White–Kauffmann–Le Minor scheme, two biochemical tests: API 20E and VITEK II, and a real-time PCR-based technique. Results: Out of 42 presumptive strains of Salmonella, 83.3% Salmonella enterica spp. enterica, 14.3% Citrobacter braakii, and 12.4% Proteus mirabilis were detected from 180 meat samples. Conclusions: Presumptive strains of Salmonella should be identified based on genotypic properties such as DNA-based methods. The aim of this study was the isolation and identification of Salmonella spp. from miscellaneous meat sorts: beef, pork, and poultry.

scholarly journals Determination of Polypeptide Antibiotic Residues in Food of Animal Origin by Ultra-High-Performance Liquid Chromatography-Tandem Mass Spectrometry

Molecules ◽  
2020 ◽  
Vol 25 (14) ◽  
pp. 3261
Author(s):  
Tomasz Bladek ◽  
Iwona Szymanek-Bany ◽  
Andrzej Posyniak
Keyword(s):  
High Performance ◽  
Multiple Reaction Monitoring ◽  
Gradient Elution ◽  
Animal Origin ◽  
Antibiotic Residues ◽  
Triple Quadrupole Mass Spectrometer ◽  
Limit Of Quantification ◽  
Food Of Animal Origin ◽  
Polypeptide Antibiotic

A novel UHPLC-MS/MS method for the determination of polypeptide antibiotic residues in animal muscle, milk, and eggs was developed and validated. Bacitracin A, colistin A, colistin B, polymyxin B1, and polymyxin B2 were extracted from the samples with a mixture of acetonitrile/water/ammonia solution 25%, 80/10/10 (v/v/v), and put through further evaporation, reconstitution, and filtration steps. The chromatographic separation was performed on a C18 column in gradient elution mode. Mass spectral acquisitions were performed in selective multiple reaction monitoring mode by a triple quadrupole mass spectrometer. The method was validated according to the criteria of Commission Decision 2002/657/EC. The method quantifies polypeptides in a linear range from 10 to 1000 μg kg−1, where the lowest concentration on the calibration curve refers to the limit of quantification (LOQ). The recoveries ranged from 70 to 99%, the repeatability was below 13%, and within-laboratory reproducibility was lower than 15%. The decision limit (CCα) and detection capability (CCβ) values were calculated, and ruggedness and stability studies were performed, to fulfill the criteria for confirmatory methods. Moreover, the developed method may also be used for screening purposes by its labor efficiency.

High Level of Antimicrobial Resistance in Campylobacter jejuni Isolated from Broiler Chickens in Estonia in 2005 and 2006

2007 ◽  
Vol 70 (8) ◽  
pp. 1940-1944 ◽  
Author(s):  
MATI ROASTO ◽  
KADRIN JUHKAM ◽  
TERJE TAMME ◽  
ARI HÖRMAN ◽  
LIIDIA HÄKKINEN ◽  
...  
Keyword(s):  
Multidrug Resistance ◽  
Antimicrobial Resistance ◽  
Campylobacter Jejuni ◽  
Broiler Chickens ◽  
Animal Origin ◽  
Campylobacter Coli ◽  
National Veterinary Institute ◽  
Food Of Animal Origin ◽  
Meat Samples ◽  
High Level

The development of antimicrobial resistance in Campylobacter jejuni and Campylobacter coli is a matter of increasing concern. Because campylobacteriosis is transmitted to humans usually via food of animal origin, the presence of antimicrobial-resistant campylobacters in broiler chickens has important public health implications. The aim of our study was to analyze resistance patterns of C. jejuni isolated from fecal samples collected at a large Estonian chicken farm, from cecal contents collected at slaughterhouses, and from meat samples collected at the retail establishments in 2005 and 2006. A total of 131 C. jejuni isolates were collected over a 13-month period and tested by the broth microdilution VetMIC method (National Veterinary Institute, Uppsala, Sweden) to determine the MICs of various antimicrobials. Resistance to one or more antimicrobials was detected in 104 (79.4%) of the 131 isolates. High proportions of the isolates were resistant to enrofloxacin (73.3%) and nalidixic acid (75.6%). Multidrug resistance (resistance to three or more unrelated antimicrobials) was detected in 36 isolates (27.5%), all of which were resistant to enrofloxacin. Multidrug resistance was significantly associated with enrofloxacin resistance (P < 0.01), and the use of enrofloxacin may select for multiresistant strains.

scholarly journals Pesticide Residues in Selected Food Commodities: Results from the Danish National Pesticide Monitoring Program 1995-1996

1999 ◽  
Vol 82 (2) ◽  
pp. 337-358 ◽  
Author(s):  
René K Juhler ◽  
Milter Green Lauridsen ◽  
Mette Rindom Christensen ◽  
Gudrun Hilbert
Keyword(s):  
Fruits And Vegetables ◽  
Monitoring Program ◽  
Animal Origin ◽  
Chlorinated Pesticides ◽  
Animal Products ◽  
Pesticide Monitoring ◽  
Food Of Animal Origin ◽  
Fish Samples ◽  
Food Commodities ◽  
Meat Samples

Abstract The paper presents results of analyses of 4182 samples collected from January 1995 to December 1996 for the Danish National Pesticide Monitoring Program. The program basis is a random sample control supplemented with a target control. The objectives of the control are to monitor consumer exposure to health hazards and to enforce national and European Community regulations. The Danish Veterinary and Food Administration is responsible for establishing methods, data manipulation, and evaluation, but actual analyses are performed at 4 regional laboratories. In addition to a description of the Danish National Pesticide Monitoring Program on food, the effects of lowering the reporting limits are discussed. Pesticides included are those in current use as well as chlorinated pesticides like lindane, DDT, and HCB, occurring in food now primarily as a result of environmental contamination. Commodities analyzed are fruits, vegetables, cereals, bran, fish, and animal products such as meat, butter, cheese, fat, and eggs. In fruits and vegetables, residues were detected in 10% of 2515 samples, with higher incidences of detection for foreign commodities. Violation rate was 0.6%. In food of animal origin, low levels of organo- chlorine pesticides were detected in most fish samples and in more than half of the animal product samples. However, no results exceeding maximum residue limits were found. No residues of organo- phosphorus pesticides were detected in the 231 meat samples analyzed.

scholarly journals Antibiotic Susceptibility Pattern and Plasmid Profiling of Some Isolates Obtained from Raw Meats Sold in Ibadan Metropolis

2017 ◽  
Vol 7 (4) ◽  
pp. 13
Author(s):  
Folake Titilayo Afolabi ◽  
A.R. Arowosebe ◽  
S.M. Adeyemo
Keyword(s):  
Multidrug Resistant ◽  
Shigella Dysenteriae ◽  
Occurrence Rate ◽  
Animal Origin ◽  
Food Of Animal Origin ◽  
Need To Evaluate ◽  
Microbiological Techniques ◽  
Plasmid Profiling ◽  
Meat Samples ◽  
Raw Meats

This study was carried out to monitor the prevalence rate of pathogenic isolates from fresh raw meats sold at butcher open shops in Ibadan metropolis, Oyo State, Nigeria. 16 samples of pork, goat, beef and chicken meats were obtained from four market locations and analyzed using standard microbiological techniques. The result of the microbiological examination of the meat samples shows the occurrence of 12 genera of bacteria out of the 161 isolates obtained: Salmonella, Shigella, Staphylococcus, Micrococcus, Enterococcus, Streptococcus, Yersinia, Proteus, Escherichia, Paracolons, Klebsiella, and Enterobacter. A total of 50 isolates were selected across the locations and tested against different antibiotics which include; ceftazidime, cefuroxime, gentamicin, ofloxacin, augmentin, tetracycline, nalidixic acid, chloramphenicol, and trimethoprim, out of which 58% of the isolates were most resistance to tetracycline, and 94% were most susceptible to gentamicin. Furthermore, 10 most antibiotic resistant isolates were selected across the four locations to check for their plasmid profiles, and four were found to contain plasmid of different sizes and numbers. These isolates were cured of their plasmids and subjected against the selected antibiotics. The isolates remained resistant to the antibiotics except for Shigella dysenteriae which becomes susceptible to the antibiotics it was formerly resistant to, showing that the gene(s) responsible for the resistance against the tested antibiotics is plasmid related. The findings of this study confirmed the presence of probable pathogenic organisms which are multidrug resistant microorganisms in raw meats sold in Ibadan metropolis, Nigeria. It significantly points to the great need to evaluate and monitor the occurrence rate of multidrug resistant organisms in livestock sold in Nigeria. There is also the need for proper and adequate cooking of food of animal origin prior consumption.

scholarly journals Identification, confirmation and quantification of sulfonamides in kidney and meat of food-producing animals by HPLC-PDA

2018 ◽  
Vol 49 (2) ◽  
pp. 115
Author(s):  
A. Ε. TIRPENOU ◽  
A. D. TSIGOURI (Α. Δ. ΤΣΙΓΟΥΡΗ) ◽  
E. H. GOUTA (E.H. ΓΟΥΤΑ)
Keyword(s):  
Mobile Phase ◽  
Solid Phase ◽  
Animal Husbandry ◽  
Animal Origin ◽  
Array Detector ◽  
Reference Laboratory ◽  
Sulfa Drug ◽  
Food Of Animal Origin ◽  
Drug Determination ◽  
Meat Samples

Residue monitoring in animal husbandry and in food of animal origin is based on the implementation of a Surveillance Scheme, according to the legislation (Directive 86/469/EEC1, N. D. 497/89% Regulation (EEC) 2377/903, Directive 96/23/EU4). Among our obligations as a European Union National Reference Laboratory (EU-NRL), we have to develope screening and confirmatory methods. Towards the development and validation of these confirmatory techniques, als for the group of antimicrobials, a method has been developed for sulfa drug determination. These substances are used extensively in livestock for therapeutic as well as for growth promotin purposes. Diserens et al., 19915 method with our modifications, is used in our laboratory to confirm the Four Plate Test6 suspect kidney and meat samples sent from the Regional Field Laboratories (RFLs) of the Ministry of Agriculture. The method's steps are: extraction, concentration and quantitative elution of sulfa drugs using Solid Phase Extraction (SPE) and their identification, confirmation and quantitation by HPLC/UV with a Photo Diode Array detector. The column used was Hypersil ODS, 5 μιη (250 x 4.6 mm) and the mobile phase was 0.01 M ammonium acetate buffer pH 4.6/MeOH at a gradient mode. The wavelength was monitored at 266 nm, the flow rate of the mobile phase was l.lmL.min ' and the injection volume was 20 μL. 12 sulfonamides can be simultaneously detected in one run at concentrations as low as 20 μg/kg. The recovery ranges from 60 to 88% for spiked samples at the Maximum Residue Limit of 100 μg/kg.

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