scholarly journals Simultaneous Morphology, Motility, and Fragmentation Analysis of Live Individual Sperm Cells for Male Fertility Evaluation

2021 ◽  
pp. 2100200
Author(s):  
Keren Ben-Yehuda ◽  
Simcha K. Mirsky ◽  
Mattan Levi ◽  
Itay Barnea ◽  
Inbal Meshulach ◽  
...  
Keyword(s):  
Male Fertility ◽  
Sperm Cells

scholarly journals LINCking the Nuclear Envelope to Sperm Architecture

Genes ◽  
2021 ◽  
Vol 12 (5) ◽  
pp. 658
Author(s):  
Francesco Manfrevola ◽  
Florian Guillou ◽  
Silvia Fasano ◽  
Riccardo Pierantoni ◽  
Rosanna Chianese
Keyword(s):  
Nuclear Envelope ◽  
Male Fertility ◽  
Nuclear Architecture ◽  
Sperm Cells ◽  
Bridge Structure ◽  
Linc Complex ◽  
Head Formation ◽  
Morphological Maturation ◽  
Integral Proteins ◽  
Sun Domain

Nuclear architecture undergoes an extensive remodeling during spermatogenesis, especially at levels of spermatocytes (SPC) and spermatids (SPT). Interestingly, typical events of spermiogenesis, such as nuclear elongation, acrosome biogenesis, and flagellum formation, need a functional cooperation between proteins of the nuclear envelope and acroplaxome/manchette structures. In addition, nuclear envelope plays a key role in chromosome distribution. In this scenario, special attention has been focused on the LINC (linker of nucleoskeleton and cytoskeleton) complex, a nuclear envelope-bridge structure involved in the connection of the nucleoskeleton to the cytoskeleton, governing mechanotransduction. It includes two integral proteins: KASH- and SUN-domain proteins, on the outer (ONM) and inner (INM) nuclear membrane, respectively. The LINC complex is involved in several functions fundamental to the correct development of sperm cells such as head formation and head to tail connection, and, therefore, it seems to be important in determining male fertility. This review provides a global overview of the main LINC complex components, with a special attention to their subcellular localization in sperm cells, their roles in the regulation of sperm morphological maturation, and, lastly, LINC complex alterations associated to male infertility.

scholarly journals Weighted single-step GWAS identified candidate genes associated with semen traits in a Duroc boar population

BMC Genomics ◽  
2019 ◽  
Vol 20 (1) ◽  
Author(s):  
Ning Gao ◽  
Yilong Chen ◽  
Xiaohong Liu ◽  
Yunxiang Zhao ◽  
Lin Zhu ◽  
...  
Keyword(s):  
Candidate Genes ◽  
Sperm Motility ◽  
Male Fertility ◽  
Snp Array ◽  
Single Step ◽  
Sperm Cells ◽  
Phenotypic Variance ◽  
Morphological Abnormalities ◽  
Genetic Variances ◽  
Progressive Motility

Abstract Background In the pig production industry, artificial insemination (AI) plays an important role in enlarging the beneficial impact of elite boars. Understanding the genetic architecture and detecting genetic markers associated with semen traits can help in improving genetic selection for such traits and accelerate genetic progress. In this study, we utilized a weighted single-step genome-wide association study (wssGWAS) procedure to detect genetic regions and further candidate genes associated with semen traits in a Duroc boar population. Overall, the full pedigree consists of 5284 pigs (12 generations), of which 2693 boars have semen data (143,113 ejaculations) and 1733 pigs were genotyped with 50 K single nucleotide polymorphism (SNP) array. Results Results show that the most significant genetic regions (0.4 Mb windows) explained approximately 2%~ 6% of the total genetic variances for the studied traits. Totally, the identified significant windows (windows explaining more than 1% of total genetic variances) explained 28.29, 35.31, 41.98, and 20.60% of genetic variances (not phenotypic variance) for number of sperm cells, sperm motility, sperm progressive motility, and total morphological abnormalities, respectively. Several genes that have been previously reported to be associated with mammal spermiogenesis, testes functioning, and male fertility were detected and treated as candidate genes for the traits of interest: Number of sperm cells, TDRD5, QSOX1, BLK, TIMP3, THRA, CSF3, and ZPBP1; Sperm motility, PPP2R2B, NEK2, NDRG, ADAM7, SKP2, and RNASET2; Sperm progressive motility, SH2B1, BLK, LAMB1, VPS4A, SPAG9, LCN2, and DNM1; Total morphological abnormalities, GHR, SELENOP, SLC16A5, SLC9A3R1, and DNAI2. Conclusions In conclusion, candidate genes associated with Duroc boars’ semen traits, including the number of sperm cells, sperm motility, sperm progressive motility, and total morphological abnormalities, were identified using wssGWAS. KEGG and GO enrichment analysis indicate that the identified candidate genes were enriched in biological processes and functional terms may be involved into spermiogenesis, testes functioning, and male fertility.

scholarly journals GAMBARAN SPERMIOGRAM PENDERITA INFERTIL DENGAN VARIKOKEL

2015 ◽  
Vol 3 (3) ◽  
Author(s):  
Piere Tendean ◽  
Lydia Tendean ◽  
Benny Wantouw
Keyword(s):  
Observational Study ◽  
Sperm Motility ◽  
Male Fertility ◽  
Sperm Morphology ◽  
Sperm Cells ◽  
Microscopical Examination ◽  
Cross Sectional ◽  
Cross Sectional Design ◽  
Infertile Patients

Abstract: Spermiogram is an examination for determination of male fertility. Evaluation of a spermiogram consists of macroscopical and microscopical examination of sperm cells, which are concentration, motility and morphology of sperm cells. This study aimed to obtain the spermiogram of infertile patients with varicocele. This was an observational study with a cross-sectional design by using sperm samples of 30 infertile patients with varicocele. Evaluation of spermatozoa quaity was determined by using WHO standard 2010. The results showed that the sperm concentrations were <15 million/ml, sperm motility <40% /field, and sperm morphology <30%/field. Conclusion: In this study the spermiogram of infertile patients with varicocele was abnormal with oligozoospermi, asthenozoospermi, and teratozoospermi.Keywords: varicocele, spermiogramAbstrak: Spermiogram merupakan salah satu pemeriksaan untuk menentukan fertilitas seorang pria. Evaluasi spermiogram meliputi makroskopik dan mikroskopik sel spermatozoa yaitu konsentrasi, motilitas, dan morfologi sel spermatozoa. Penelitian ini bertujuan untuk mendapatkan gambaran spermiogram penderita infertil dengan varikokel. Penelitian ini bersifat observasional dengan desain potong lintang dengan menggunakan sampel sperma dari 30 penderita infertil dengan varikokel. Evaluasi kualitas spermatozoa berdasarkan standard WHO 2010. Hasil penelitian ini didapatkan yaitu konsentrasi sperma <15 juta/ml, motilitas sperma <40%/lp, dan morfologi sperma < 30%/lp. Simpulan: Pada penelitian ini gambaran spermiogram penderita infertil dengan varikokel ialah abnormal dengan oligozoospermi, asthenozoospermi, dan teratozoospermi. Kata kunci: varikokel, spermiogram

scholarly journals Oxidation of Sperm Nucleus in Mammals: A Physiological Necessity to Some Extent with Adverse Impacts on Oocyte and Offspring

Antioxidants ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 95 ◽  
Author(s):  
Joël R. Drevet ◽  
Robert John Aitken
Keyword(s):  
Reactive Oxygen Species ◽  
Clinical Practice ◽  
Oxidative Damage ◽  
Male Fertility ◽  
Sperm Nucleus ◽  
Sperm Cells ◽  
Oxygen Species ◽  
Adverse Impacts ◽  
Sperm Dna ◽  
Cellular Components

Sperm cells have long been known to be good producers of reactive oxygen species, while they are also known to be particularly sensitive to oxidative damage affecting their structures and functions. As with all organic cellular components, sperm nuclear components and, in particular, nucleic acids undergo oxidative alterations that have recently been shown to be commonly encountered in clinical practice. This review will attempt to provide an overview of this situation. After a brief coverage of the biological reasons why the sperm nucleus and associated DNA are sensitive to oxidative damage, a summary of the most recent results concerning the oxidation of sperm DNA in animal and human models will be presented. The study will then attempt to cover the possible consequences of sperm nuclear oxidation on male fertility and beyond.

scholarly journals Simultaneous Morphology, Motility and Fragmentation Analysis of Live Individual Sperm Cells for Male Fertility Evaluation

2021 ◽  
Author(s):  
Keren Ben-Yehuda ◽  
Simcha K Mirsky ◽  
Mattan Levi ◽  
Itay Barnea ◽  
Inbal Meshulach ◽  
...  
Keyword(s):  
Deep Learning ◽  
Dna Fragmentation ◽  
Male Fertility ◽  
Human Sperm ◽  
Sperm Cells ◽  
Interferometric Imaging ◽  
Vitro Fertilization ◽  
Lack Of Information ◽  
Common Clinical Practice

We present a new technique for simultaneously analyzing morphology, motility and DNA fragmentation of live human sperm cells at the single-cell level for male fertility evaluation. It relies on quantitative stain-free interferometric imaging and multiple deep-learning frameworks. In the common clinical practice, only motility evaluation is carried out on live human cells, while full morphological evaluation and DNA fragmentation assays require different staining protocols, and therefore cannot be performed simultaneously on the same cell. This results in a lack of information regarding the intersection of these scores. We use a clinic-ready interferometric module and deep learning to acquire dynamic sperm cells without chemical staining, and evaluate all three scores per each cell together with virtual staining. We show that the number of cells that pass each criterion separately does not accurately predict how many would pass all criteria, thus the triple evaluation per cell is necessary for accurate fertility grading. This stain-free evaluation is expected to decrease the uncertainty in male fertility evaluation, as well as be applied for sperm selection during in vitro fertilization.

scholarly journals TESTICULAR VOLUME

2015 ◽  
Vol 22 (10) ◽  
pp. 1356-1361
Author(s):  
Hina Khan ◽  
Mazhur-Ul- Haque ◽  
Muhammad Rafique ◽  
Asad Raza Jiskani
Keyword(s):  
Body Weight ◽  
Male Fertility ◽  
Petri Dish ◽  
Health Sciences ◽  
Albino Rats ◽  
Sperm Cells ◽  
Group A ◽  
Study Institute ◽  
The Mean ◽  
Group B

Objective: To determinate that male fertility influenced by testicular volumein albino rats. Study Design: Experimental. Place and Duration of study: Institute of BasicMedical Sciences, Dow University of Health Sciences, Karachi, 10 months (November 2009to August 2010). Methodology: Sixty four adult albino rats were obtained from animal houseJinnah Postgraduate Medical Centre for the study and divided into 2 groups. Group A receivedinjection normal saline 1 cc intraperitoneally (IP) daily for 8 weeks. Group B received leadchloride in a dose of 10 mg/kg body weight IP daily. On the day of completion of treatmentthe animals were sacrificed testes along with epididymis removed and place in Petri dish. Thelength, breath and width of testes were measured with help of vernier caliper. The spermatozoawere obtained from cauda epididymis. Results: The mean ± SEM of volume testes in groupA and B after eight week of treatment were 0.77142 ± 0.04778 cm3 & 0.11768 ± 0.01673 cm3respectively. The volume of testes of group B was significantly decreased as compare to groupB (P = 0.000). Mean ±. The mean ± SEM number of sperm cells million / ml in groups A andB after eight week of treatment was 7.65 ± 186706.553 & 1.84 ± 132792.770 respectively.Number of sperms in group B were significantly decreased as compared to group A (P =0.000). Conclusion: There was relationship between volume of testes and male fertility.

scholarly journals Male fertility: a review of the publications July — September 2021

2021 ◽  
Vol 9 (4) ◽  
pp. 147-155
Author(s):  
D. S. Rogozin
Keyword(s):  
Male Fertility ◽  
Selection Criteria ◽  
Chromosomal Abnormalities ◽  
Paternal Age ◽  
Practical Significance ◽  
Sperm Cells ◽  
The Novel ◽  
The Impact ◽  
Scimago Journal Rank

The article provides an overview of the most significant publications on the topic of male infertility. The main selection criteria were considered the practical significance of the article, as well as the impact factor of the journal in which it was published according to the SCImago Journal Rank (SJR). As a result, we formed a list of 10 articles published in the III quarter (July — September) of 2021. The review included articles concerning the following issues: the ability of oocytes to repair damaged DNA-chains of sperm cells, the effectiveness of ICSI in AZF-c microdeletions, the advanced paternal age, artificial intelligence in reproductive clinics, genetic causes of infertility, the effect of surgical treatment of varicocele concerning DNA fragmentation, the role of ICSI in the frequency of chromosomal abnormalities in offspring, the safety of COVID-19 vaccination for spermatogenesis, as well as the novel WHO 6 manual for semen investigation.

Daily exposure to summer temperatures affects the motile subpopulation structure of epididymal sperm cells but not male fertility in an in vivo rabbit model

2015 ◽  
Vol 84 (3) ◽  
pp. 384-389 ◽  
Author(s):  
M.J. Maya-Soriano ◽  
E. Taberner ◽  
M. Sabés-Alsina ◽  
J. Ramon ◽  
O. Rafel ◽  
...  
Keyword(s):  
Male Fertility ◽  
Rabbit Model ◽  
Sperm Cells ◽  
Epididymal Sperm ◽  
Daily Exposure ◽  
Summer Temperatures

scholarly journals 291PRESENCE OF PLATELET-ACTIVATING FACTOR (PAF) RECEPTOR IN BULL SPERM AND POSITIVE CORRELATION OF SPERM PAF CONTENT WITH FERTILITY

2004 ◽  
Vol 16 (2) ◽  
pp. 265 ◽  
Author(s):  
B.G. Brackett ◽  
P. Bosch ◽  
R.A. McGraw ◽  
J.M. DeJarnette ◽  
C.E. Marshall ◽  
...  
Keyword(s):  
Reverse Transcription ◽  
Male Fertility ◽  
Platelet Activating Factor ◽  
Receptor Expression ◽  
Epifluorescence Microscopy ◽  
Receptor Protein ◽  
Sperm Cells ◽  
Positive Correlation ◽  
Paf Receptor ◽  
Bull Sperm

Male fertility involves the capacity to obtain viable pregnancy and offspring after insemination. Currently, the most common way to measure bull fertility is through non-return rates (NRR) calculated after insemination of many females. However, this method is time-consuming and expensive. A number of biochemical molecules in sperm have been proposed as potential predictors of male fertility, e.g. platelet-activating factor (PAF). Platelet-activating factor (1-O-alkyl-2-O-acetyl-sn-glycero-3-phosphorylcholine) is a ubiquitous phospholipid that is implicated in the mediation of a wide variety of reproductive processes. The mechanism of PAF’s action is a receptor-mediated event reported to affect intracellular calcium levels. Bull sperm contain PAF and its content has a positive relationship with motility. While the PAF-receptor has been reported in other species, it has not been demonstrated in bull sperm. Therefore, our objectives were to determine: 1. the relationship between PAF content in bull sperm and Estimated Relative Conception Rates (ERCR, a 3-year rolling average of NRR); and 2. the presence of the PAF-receptor in bull sperm. Sperm PAF content for bulls (n=8) with different ERCR was determined by radioimmunoassay. PAF-receptor expression was determined as follows: total RNA was purified by acid phenol extraction and ethanol precipitation. Complementary DNAs were synthesized by reverse transcriptase with dNTPs and random primers at 37°C, 60min; followed by 65°C, 5min. Reverse transcription (cDNA) products were amplified with Taq polymerase, dNTP, and PAF receptor primer pair (upper, 5′-AATCCAGTCACCCTGGTTGTAG-3′; lower, 5′-TGGACTCAGAGTTCCGATACAC-3′) at 94°C, 1min; 55°C, 1min; 72°C, 1min for 35 cycles followed by 72°C, 7min. RT-PCR products were analyzed by 2% agarose gel electrophoresis. PAF-receptor protein was determined as follows: PBS-washed bull sperm was exposed to human PAF-receptor antibody at 4°C for 3h, washed in PBS, then exposed to fluorescein isothiocyanate-conjugated anti-IgG for 90min at 37°C, and again washed in PBS. Specimens were examined by epifluorescence microscopy at 400×. PAF content in bull sperm ranged from 1.39ng/106 sperm cells to 13.68ng/106 sperm cells. There was a positive correlation (P&lt;0.05) between PAF content and ERCR. Presence of PAF-receptors in bull sperm was confirmed by immunofluorescence. However, distribution of PAF-receptors in bull sperm was not uniform within or between specimens. A cDNA clone containing the coding region for PAF-receptor was isolated from bull sperm using a reverse transcription-polymerase chain reaction protocol. There is a positive correlation (R=0.40; P&lt;0.05) between PAF content in sperm and in vivo fertility of individual bulls as determined by NRR. Molecular and immunofluorescence data confirm the presence of PAF-receptor (mRNA and protein) in bull sperm. Additional studies are warranted to elucidate the mechanism of PAF’s action in sperm. Early selection for fertility in bulls represents a potentially valuable application to enhance efficiency in cattle breeding.

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