scholarly journals GAMBARAN SPERMIOGRAM PENDERITA INFERTIL DENGAN VARIKOKEL

2015 ◽  
Vol 3 (3) ◽  
Author(s):  
Piere Tendean ◽  
Lydia Tendean ◽  
Benny Wantouw
Keyword(s):  
Observational Study ◽  
Sperm Motility ◽  
Male Fertility ◽  
Sperm Morphology ◽  
Sperm Cells ◽  
Microscopical Examination ◽  
Cross Sectional ◽  
Cross Sectional Design ◽  
Infertile Patients

Abstract: Spermiogram is an examination for determination of male fertility. Evaluation of a spermiogram consists of macroscopical and microscopical examination of sperm cells, which are concentration, motility and morphology of sperm cells. This study aimed to obtain the spermiogram of infertile patients with varicocele. This was an observational study with a cross-sectional design by using sperm samples of 30 infertile patients with varicocele. Evaluation of spermatozoa quaity was determined by using WHO standard 2010. The results showed that the sperm concentrations were <15 million/ml, sperm motility <40% /field, and sperm morphology <30%/field. Conclusion: In this study the spermiogram of infertile patients with varicocele was abnormal with oligozoospermi, asthenozoospermi, and teratozoospermi.Keywords: varicocele, spermiogramAbstrak: Spermiogram merupakan salah satu pemeriksaan untuk menentukan fertilitas seorang pria. Evaluasi spermiogram meliputi makroskopik dan mikroskopik sel spermatozoa yaitu konsentrasi, motilitas, dan morfologi sel spermatozoa. Penelitian ini bertujuan untuk mendapatkan gambaran spermiogram penderita infertil dengan varikokel. Penelitian ini bersifat observasional dengan desain potong lintang dengan menggunakan sampel sperma dari 30 penderita infertil dengan varikokel. Evaluasi kualitas spermatozoa berdasarkan standard WHO 2010. Hasil penelitian ini didapatkan yaitu konsentrasi sperma <15 juta/ml, motilitas sperma <40%/lp, dan morfologi sperma < 30%/lp. Simpulan: Pada penelitian ini gambaran spermiogram penderita infertil dengan varikokel ialah abnormal dengan oligozoospermi, asthenozoospermi, dan teratozoospermi. Kata kunci: varikokel, spermiogram

Profil Penyakit Malaria Di Rumah Sakit Islam Siti Rahmah Padang Tahun 2018

2020 ◽  
Vol 2 (2) ◽  
pp. 08-15
Author(s):  
Rahma Triyana ◽  
Salmi Salmi
Keyword(s):  
Observational Study ◽  
Frequency Distribution ◽  
Cross Section ◽  
Laboratory Tests ◽  
Age Group ◽  
Normal Range ◽  
Cross Sectional ◽  
West Sumatra ◽  
Severity Of The Disease

Malaria is one of the health problems in Indonesia, especially West Sumatra. Determination of the description of Malaria disease in an area is needed to determine the spread and severity of the disease. This study aims to determine the frequency distribution according to age, sex and place of residence, description of the types of Plasmodium causes of Malaria and hematological features in Malaria patients at Siti Rahmah Padang Hospital in 2018. This type of research is a descriptive observational study with an approach or design cross section (cross sectional). The frequency distribution of Malaria sufferers in Siti Rahmah Padang Hospital in 2018 according to the highest age was in the age group 21-30 years as many as 28 cases (36.8%), the highest sex among men was 46 (60.5%) and the highest number of residences was found in Koto Tangah sub-district there were 31 cases (40.8%). The type of Plasmodium found in Malaria cases in Siti Rahmah Padang Hospital in 2018 was P. vivax (73 cases (96.05%)) and P. falciparum (3 cases (3.95%)). The results of laboratory tests on Hb, hematocrit, platelet and leukocyte levels in Malaria positive patients in Siti Rahmah Padang Hospital in 2018 were in the normal range.

Profil Penyakit Malaria di Rumah Sakit Islam Siti Rahmah Padang Tahun 2018

2020 ◽  
Vol 2 (2) ◽  
pp. 08-15
Author(s):  
Rahma Triyana Y ◽  
Salmi Salmi
Keyword(s):  
Observational Study ◽  
Frequency Distribution ◽  
Cross Section ◽  
Laboratory Tests ◽  
Age Group ◽  
Normal Range ◽  
Cross Sectional ◽  
West Sumatra ◽  
Severity Of The Disease

Malaria is one of the health problems in Indonesia, especially West Sumatra. Determination of the description of Malaria disease in an area is needed to determine the spread and severity of the disease. This study aims to determine the frequency distribution according to age, sex and place of residence, description of the types of Plasmodium causes of Malaria and hematological features in Malaria patients at Siti Rahmah Padang Hospital in 2018. This type of research is a descriptive observational study with an approach or design cross section (cross sectional). The frequency distribution of Malaria sufferers in Siti Rahmah Padang Hospital in 2018 according to the highest age was in the age group 21-30 years as many as 28 cases (36.8%), the highest sex among men was 46 (60.5%) and the highest number of residences was found in Koto Tangah sub-district there were 31 cases (40.8%). The type of Plasmodium found in Malaria cases in Siti Rahmah Padang Hospital in 2018 was P. vivax (73 cases (96.05%)) and P. falciparum (3 cases (3.95%)). The results of laboratory tests on Hb, hematocrit, platelet and leukocyte levels in Malaria positive patients in Siti Rahmah Padang Hospital in 2018 were in the normal range.

scholarly journals Weighted single-step GWAS identified candidate genes associated with semen traits in a Duroc boar population

BMC Genomics ◽  
2019 ◽  
Vol 20 (1) ◽  
Author(s):  
Ning Gao ◽  
Yilong Chen ◽  
Xiaohong Liu ◽  
Yunxiang Zhao ◽  
Lin Zhu ◽  
...  
Keyword(s):  
Candidate Genes ◽  
Sperm Motility ◽  
Male Fertility ◽  
Snp Array ◽  
Single Step ◽  
Sperm Cells ◽  
Phenotypic Variance ◽  
Morphological Abnormalities ◽  
Genetic Variances ◽  
Progressive Motility

Abstract Background In the pig production industry, artificial insemination (AI) plays an important role in enlarging the beneficial impact of elite boars. Understanding the genetic architecture and detecting genetic markers associated with semen traits can help in improving genetic selection for such traits and accelerate genetic progress. In this study, we utilized a weighted single-step genome-wide association study (wssGWAS) procedure to detect genetic regions and further candidate genes associated with semen traits in a Duroc boar population. Overall, the full pedigree consists of 5284 pigs (12 generations), of which 2693 boars have semen data (143,113 ejaculations) and 1733 pigs were genotyped with 50 K single nucleotide polymorphism (SNP) array. Results Results show that the most significant genetic regions (0.4 Mb windows) explained approximately 2%~ 6% of the total genetic variances for the studied traits. Totally, the identified significant windows (windows explaining more than 1% of total genetic variances) explained 28.29, 35.31, 41.98, and 20.60% of genetic variances (not phenotypic variance) for number of sperm cells, sperm motility, sperm progressive motility, and total morphological abnormalities, respectively. Several genes that have been previously reported to be associated with mammal spermiogenesis, testes functioning, and male fertility were detected and treated as candidate genes for the traits of interest: Number of sperm cells, TDRD5, QSOX1, BLK, TIMP3, THRA, CSF3, and ZPBP1; Sperm motility, PPP2R2B, NEK2, NDRG, ADAM7, SKP2, and RNASET2; Sperm progressive motility, SH2B1, BLK, LAMB1, VPS4A, SPAG9, LCN2, and DNM1; Total morphological abnormalities, GHR, SELENOP, SLC16A5, SLC9A3R1, and DNAI2. Conclusions In conclusion, candidate genes associated with Duroc boars’ semen traits, including the number of sperm cells, sperm motility, sperm progressive motility, and total morphological abnormalities, were identified using wssGWAS. KEGG and GO enrichment analysis indicate that the identified candidate genes were enriched in biological processes and functional terms may be involved into spermiogenesis, testes functioning, and male fertility.

scholarly journals PRAKTIK HIGIENE PERORANGAN DAN SANITASI WARUNG PECEL TUMPANG DI KOTA KEDIRI

IKESMA ◽  
2017 ◽  
Vol 13 (2) ◽  
Author(s):  
Gading Giovani PutriPutri
Keyword(s):  
Food Safety ◽  
Observational Study ◽  
Waste Disposal ◽  
Personal Hygiene ◽  
Clean Water ◽  
Cross Sectional ◽  
Cross Sectional Design

To realize food safety is applying good personal hygiene and sanitation.This study aims to describe personal hygiene and foodstalls sanitation of pecel tumpang in Kediri.An observational study with cross sectional design done on 20 sampels. Data performed in table with descriptive analyzed. Most of respondents had quite personal hygiene (80%), good location and building (50%), poor waste disposal (50%), poor sanitation facilities(50%), good sanitation of equipments (100%), and good clean water (90%).Somevariables indicate poor sanitation i.e. waste disposal and sanitation facilities. Keywords: personal hygiene, food stalls sanitation, pecel tumpang

scholarly journals Perbedaan Kadar Malondialdehide dan Tromboksan B2 pada Remaja dengan Dismenore dan Tanpa Dismenore

2015 ◽  
Vol 4 (3) ◽  
Author(s):  
Berliana Irianti ◽  
Ermawati Ermawati ◽  
Arni Amir
Keyword(s):  
Lipid Peroxidation ◽  
Observational Study ◽  
Thiobarbituric Acid ◽  
Sample Analysis ◽  
Cross Sectional ◽  
Significant Difference ◽  
The Mean ◽  
The Difference ◽  
Cross Sectional Design ◽  
Biomedical Laboratory

Abstrak Penyebab dismenore belum semuanya diketahui, ada dugaan peningkatan proses peroksida lipid yang akan mengaktivasi mediator inflamasi pada endometrium yang menimbulkan rasa nyeri haid (dismenore). Tujuan penelitian ini adalah menentukan perbedaan kadar malondialdehide dan tromboksan B 2  pada dismenore dan tanpa dismenore. Studi observasional ini menggunakan desain potong lintang komparatif. Subjek penelitian terdiri dari dua kelompok yaitu 23 remaja dismenore dan 23 remaja tanpa dismenore dengan waktu penelitian dari Juni sampai Juli 2014. Analisis sampel dilakukan di Laboratorium Biomedik dan Biokimia Universitas Andalas Padang. Pemeriksaan kadartromboksan B 2  menggunakan metode ELISA dan kadar malondialdehide menggunakan metode Asam Thiobarbiturat (TBA). Hasil penelitian diperoleh bahwa rerata kadar malondialdehid pada remaja dengan dismenore yaitu 2,60±0,63 µmol/ml, rerata remaja tanpa dismenore 1,98±0,12 µmol/ml dengan probabilitas p<0,05 (0,000), sedangkan reratakadar Tromboksan B 2  pada remaja dengan dismenore 20,043±9,56 ng/ml, rerata remaja tanpa dismenore 19,222±10,79 ng/ml, dengan probabilitas p>0,05 (0,786). Kesimpulan penelitian ini adalah terdapat perbedaan yang signifikan rerata kadar malondialdehid pada remaja dengan dismenore dan tanpa dismenore dan tidak terdapatperbedaan signifikan pada kadar tromboksan B 2 pada remaja dengan dismenore dan tanpa dismenore.Kata kunci: remaja, dismenore, malondialdehide, tromboksan B2 Abstract The precise cause of dysmenorrhea is still unclear, there may be increased lipid peroxidation process will activate the inflammatory mediators at endometrium that cause menstrual cramps (dysmenorrhea). The objective of this study was to determine the difference of malondialdehyde levels and thromboxane B 2  levels in dysmenorrhea and without dysmenorrhea. It was an observational study with comparative cross-sectional design. The subjects consisted of two groups, they are 23 adolescent with dysmenorrhea and 23 adolescents without dysmenorrhea, done in Juny -July 2014. Sample analysis was conducted in Laboratory of Biochemistry and Biomedical Laboratory of Andalas University Padang. The examination of Thromboxane B 2  levels used ELISA and the examination of malondialdehyde levels used a Thiobarbituric acid method. The results showed the mean of malondialdehyde levels in adolescents withdysmenorrhea was 2.60±0.63 µmol/ml, the mean level in adolescent without dysmenorrhea was 1.98±0.12 µmol/ml with probability p<0.05 (0.000), while the mean levels of thromboxane B 2  in adolescents with dysmenorrhea was 20.043±9.56 ng/ml, the mean level in adolescent without dysmenorrhea was 19.222±10.79 ng/ml, with probabilityp>0.05 (0.786). It can be concluded that there is a significant difference in the mean of malondialdehyde levels between adolescents with dysmenorrhea and without dysmenorrhea and there is no significant differences in thromboxane B 2 level between adolescents with dysmenorrhea and without dysmenorrheaKeywords: adolescent, dysmenorrhea, malondialdehyde, thromboxane B2

scholarly journals Hypertension: Dominant Risk Factor on Stroke Occurence Analytical Observational Study in Sultan Agung Islamic Hospital Semarang during January 1, 2012 - August 31, 2013

Sains Medika ◽  
2015 ◽  
Vol 6 (1) ◽  
pp. 8
Author(s):  
Desia Laila Dian Saputri ◽  
Ika Rosdiana ◽  
Endang Lestari
Keyword(s):  
Risk Factor ◽  
Observational Study ◽  
Sampling Technique ◽  
Simple Random Sampling ◽  
Dominant Factor ◽  
Stroke Patients ◽  
Cross Sectional ◽  
Factors Associated ◽  
Stroke Study ◽  
Cross Sectional Design

Introduction: Stroke ranks as the first cause of disabilities and the third cause of death in the world. Many factors associated with stroke. Objective: this study aimed at evaluating the factors associated with stroke. Study Design: This study was an analytical observational study using cross sectional design. Samples were obtained from medical records of 263 patients who were admitted in neurology’s ward of Sultan agung Islamic hospital during 1 January 2012-31 August 2013. Samples categorized in 2 groups: 97 hemorrhagic’s stroke patients and 166 non hemorrhagic’s stroke patients with simple random sampling technique. This study were conducted by analyzing 5 variables such as gender, age, hypertension, diabetes mellitus and total cholesterol levels. Results: Logistic regression test using SPSS showed that hypertension was the most dominant factor associated with stroke (p=0.001; PR=1.707; CI=1.249–2.433). Conclusion: Hypertension was the most dominant risk factor for stroke.

scholarly journals KESEHATAN TENGGOROK SEKOLAH MENENGAH ATAS NEGERI 9 MANADO

e-CliniC ◽  
2016 ◽  
Vol 4 (1) ◽  
Author(s):  
Gabriella A. Koagouw ◽  
Steward K. Mengko ◽  
Armenius R. Sondakh
Keyword(s):  
High School ◽  
Health Status ◽  
Observational Study ◽  
Sore Throat ◽  
Health Problem ◽  
Senior High School ◽  
Cross Sectional ◽  
10Th Grade ◽  
Cross Sectional Design

Abstract: The most throat health problem is inflammation. Around 80% of sore throat are caused by viruses and only 10-20% are caused by bacteria. The types of inflammation found on the throat are tonsillitis and pharyngitis. This study aimed to obtain data of throat health among students of SMA Negeri 9 (Senior High School) Manado. This was a descriptive observational study with a cross sectional design. Respondents were 10th grade students of SMA Negeri 9 Manado. The results showed that most respondents had normal tonsil and pharynx. Conclusion: Most students of SMA Negeri 9 Manado had good throat health status. Keywords: throat health status, tonsil examination, pharynx examination Abstrak: Masalah kesehatan pada tenggorok yang terbanyak ditemukan ialah peradangan. Sekitar 80% radang tenggorok disebabkan oleh virus dan hanya 10-20% disebabkan oleh bakteri. Peradangan yang sering ditemukan pada tenggorok ialah tonsilitis dan faringitis. Penelitian ini bertujuan untuk mendapatkan gambaran kesehatan tenggorok pada siswa-siswi Sekolah Menengah Atas (SMA) Negeri 9 Manado. Penelitian ini menggunakan metode deskriptif observasional dengan desain potong lintang. Responden ialah siswa-siswi kelas X.4 Sekolah Menengah Atas (SMA) Negeri 9 Manado. Hasil penelitian menunjukkan bahwa umumnya responden mempunyai tonsil dan faring yang normal. Simpulan: Umumnya gambaran kesehatan tenggorok pada siswa-siswi Sekolah Menengah Atas (SMA) Negeri 9 Manado baik.Kata kunci: kesehatan tenggorok, pemeriksaan tonsil, pemeriksaan faring

scholarly journals Genetic Association of the Functional WDR4 Gene in Male Fertility

2021 ◽  
Vol 11 (8) ◽  
pp. 760
Author(s):  
Yu-Jia Wang ◽  
Eko Mugiyanto ◽  
Yun-Ting Peng ◽  
Wan-Chen Huang ◽  
Wan-Hsuan Chou ◽  
...  
Keyword(s):  
Male Infertility ◽  
Genetic Association ◽  
Sperm Motility ◽  
Male Fertility ◽  
Sperm Morphology ◽  
Critical Role ◽  
Clinical Manifestations ◽  
Physiological Traits ◽  
Clinical Records ◽  
Low Expression

Infertility is one of the important problems in the modern world. Male infertility is characterized by several clinical manifestations, including low sperm production (oligozoospermia), reduced sperm motility (asthenozoospermia), and abnormal sperm morphology (teratozoospermia). WDR4, known as Wuho, controls fertility in Drosophila. However, it is unclear whether WDR4 is associated with clinical manifestations of male fertility in human. Here, we attempted to determine the physiological functions of WDR4 gene. Two cohorts were applied to address this question. The first cohort was the general population from Taiwan Biobank. Genomic profiles from 68,948 individuals and 87 common physiological traits were applied for phenome-wide association studies (PheWAS). The second cohort comprised patients with male infertility from Wan Fang Hospital, Taipei Medical University. In total, 81 male participants were recruited for the genetic association study. Clinical records including gender, age, total testosterone, follicle-stimulating hormone (FSH), luteinizing hormone (LH), total sperm number, sperm motility, and sperm morphology were collected. In the first cohort, results from PheWAS exhibited no associations between WDR4 genetic variants and 87 common physiological traits. In the second cohort, a total of four tagging single-nucleotide polymorphisms (tSNPs) from WDR4 gene (rs2298666, rs465663, rs2248490, and rs3746939) were selected for genotyping. We found that SNP rs465663 solely associated with asthenozoospermia. Functional annotations through the GTEx portal revealed the correlation between TT or TC genotype and low expression of WDR4. Furthermore, we used mouse embryonic fibroblasts cells from mwdr4 heterozygous (+/‒) mice for functional validation by western blotting. Indeed, low expression of WDR4 contributed to ROS-induced DNA fragmentation. In conclusion, our results suggest a critical role of WDR4 gene variant as well as protein expression in asthenozoospermia.

scholarly journals Optimalisasi radiografi gigi konvensional untuk membantu pemasangan implan gigi

2009 ◽  
Vol 8 (1) ◽  
pp. 11
Author(s):  
Barunawaty Yunus
Keyword(s):  
Observational Study ◽  
Panoramic Radiography ◽  
Treatment Plan ◽  
Cross Sectional ◽  
Optimal Measurement ◽  
Jaw Bone ◽  
Significant Difference ◽  
Before And After ◽  
Cross Sectional Design ◽  
Periapical Radiography

Radiography as diagnostic tool is essential for treatment plan of implant. Manytechniques used to produce radiography are recommended to support the dentist inaccessing area of implantation. Many implant experts use panoramic radiography asguidelines of jaw bone evaluation as recommended by implant procedures. In orderto get optimal measurement, occlusal radiography technique can be added to evaluatethe bone thickness.panoramic and periapical radiography can solely evaluate thewidth and height of jaw bone. This study proposed to evaluate parametric value, aswidth, height, and thickness of jaw bone by conventional dental diagnosticradiography. This was observational study, with cross sectional design. Samples werechosen as population target that fulfilled samples criteria. Number of samples were 30and and analyzed using the SPSS program for Windows 14.0 and T-test. In this study,it can be concluded that there was significant difference before and after conversion ofall parametric radiography conventional tooth on jaw bone.

scholarly journals Deteksi Endoparasit Cacing pada Hepar Tikus Laboratorium (Rattus norvegicus) dari Sentra Peternak di Kabupaten Banyumas dan Kabupaten Purbalingga

2021 ◽  
pp. 119-126
Author(s):  
Dita Pratiwi Kusuma Wardani ◽  
Kurnia Ritma Dhanti ◽  
Arif Mulyanto ◽  
Tantri Analisawati Sudarsono
Keyword(s):  
Observational Study ◽  
Health Monitoring ◽  
Liver Surgery ◽  
Rattus Norvegicus ◽  
Laboratory Animal ◽  
Cross Sectional ◽  
Laboratory Rats ◽  
Helminth Infections ◽  
Cross Sectional Design ◽  
Taenia Taeniaeformis

The presence of laboratory rats that are maintained and bred for laboratory purposes or laboratory observations is very necessary. The presence of endoparasite in laboratory rats will have an impact on the result of the research or laboratory observations. This study aims to detect helminth endoparasites in the liver of laboratory rats (Rattus norvegicus) from animal breeders in Banyumas and Purbalingga Districts. This research was an observational study with a cross-sectional design. A total of 52 laboratory rats were used in the study. Rats are killed with chloroform, liver surgery then identify the presence of worm larvae. Out of the 52 rats obtained, 7 (29.17%) from 24 laboratory rats in Banyumas District and 5 (17.86%) from 28 laboratory rats in Purbalingga District were infected with Taenia taeniaeformis. It is necessary to control helminth infections in laboratory rats, such as laboratory animal quarantine, health monitoring, and antihelmintic treatment. It is important to handle carefully during travel to assure the results of research or laboratory observations using the animals.

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