Application of decoy oligodeoxynucleotides strategy for inhibition of cell growth and reduction of metastasis properties in non‐resistant and Erlotinib‐resistant SW480 cell line

2020 ◽  
Author(s):  
Zoleykha Asadi ◽  
Mojtaba Fathi ◽  
Elham Rismani ◽  
Zahra Bigdelou ◽  
Behrooz Johari
Keyword(s):  
Cell Growth ◽  
Cell Line ◽  
Sw480 Cell ◽  
Sw480 Cell Line

Hsa-miR-11181 regulates Wnt signaling pathway through targeting of APC2 transcripts in SW480 cell line

Gene ◽  
2018 ◽  
Vol 641 ◽  
pp. 297-302 ◽  
Author(s):  
Sadat Dokanehiifard ◽  
Bahram M. Soltani
Keyword(s):  
Wnt Signaling ◽  
Cell Line ◽  
Signaling Pathway ◽  
Wnt Signaling Pathway ◽  
Sw480 Cell ◽  
Sw480 Cell Line

scholarly journals Downregulating forkhead box M1 inhibits proliferation by inhibiting autophagy in the sw480 cell line

2017 ◽  
Vol 7 (1) ◽  
pp. 47-50 ◽  
Author(s):  
Shibiao Zhong ◽  
Aiyan Zhou ◽  
Fanghua Qi ◽  
Zhen Li ◽  
Zeyan Yu ◽  
...  
Keyword(s):  
Cell Line ◽  
Sw480 Cell ◽  
Forkhead Box ◽  
Sw480 Cell Line ◽  
Forkhead Box M1

scholarly journals Mir-186-5p Regulates WNT Signaling Pathway by Targeting TCF4 Transcription Factor

2018 ◽  
Vol 8 (1) ◽  
pp. 132
Author(s):  
Zahra Bayat ◽  
Bahram M. Soltani
Keyword(s):  
Gene Expression ◽  
Cell Cycle ◽  
Wnt Signaling ◽  
Cell Line ◽  
Signaling Pathway ◽  
Wnt Pathway ◽  
Wnt Signaling Pathway ◽  
Sw480 Cell ◽  
Sw480 Cell Line ◽  
Sw480 Cells

The evolutionarily conserved Wnt signaling pathway plays essential roles during embryonic development, tissue homeostasis and differentiation. This pathway is deregulated in many cancers especially colorectal cancer. MiRNAs are a class of small noncoding RNAs that may play a major role in post transcriptional regulation of many genes and signaling pathway such as WNT signaling pathway. Here, we intended to investigate if miR-186-5p is capable of regulating WNT signaling pathway wia suppression TCF4 gene expression. miR-186-5p was bioinformatically predicted as a candidate regulator of TCF4 gene expression and then, in this experimental study, miR-186-5p was overexpressed in SW480 cell line and its increased expression was detected through quantitative reverse transcription polymerase chain reaction (RT-qPCR). The effect of miR-186-5p on WNT pathway was analysied with TOP/FOP flash assay in SW480 cell line. Finally, flow cytometery was used to inves tigate the effect of miR-186-5p overexpression on cell cycle progression in SW480 cell line. miR-186-5p was overexpressed in the SW480 cell line and its overexpression resulted in significant reduction of the TCF4 mRNA level. TOP/FOP flash assay, confirmed the negative effect of miR-186-5p on the Wnt pathway in SW480 cells. Finally, Overexpression of miR186-5p in SW480 cells resulted in cell cycle arrest in subG1 phase, detected by flow cytometry. Overall, accumulative results indi-cated that miR-186-5p by targeting TCF4 is potentially one of the regulators of the WNT signaling pathway.

scholarly journals Effect of camptothecin on inducible nitric oxide synthase expression in the colon cancer SW480 cell line

2015 ◽  
Vol 10 (5) ◽  
pp. 3157-3160 ◽  
Author(s):  
XIANGDI SHEN ◽  
JIAN CHEN ◽  
RONG QIU ◽  
XINGLI FAN ◽  
YING XIN
Keyword(s):  
Nitric Oxide ◽  
Colon Cancer ◽  
Nitric Oxide Synthase ◽  
Cell Line ◽  
Inducible Nitric Oxide Synthase ◽  
Sw480 Cell ◽  
Sw480 Cell Line ◽  
Oxide Synthase ◽  
Inducible Nitric Oxide

scholarly journals Combination Therapy with KRAS and P38α siRNA Suppresses Colorectal Cancer Growth and Development in SW480 Cell Line

2021 ◽  
Author(s):  
Shiva Kamran ◽  
Ensiyeh Seyedrezazadeh ◽  
Dariush Shanehbandi ◽  
Milad Asadi ◽  
Venus Zafari ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Combination Therapy ◽  
Cell Line ◽  
Growth And Development ◽  
Sw480 Cell ◽  
Cancer Growth ◽  
Sw480 Cell Line

scholarly journals Wnt Signaling and Cadherin Expressions in Different Staging of Colorectal Cancer as Biomarkers for Metastasis: Study of SW480, COLO 320DM, and HCT116 Cellines

2021 ◽  
Vol 9 (B) ◽  
pp. 763-770
Author(s):  
Luminturahardjo Winarko ◽  
Pudji Rahajoe ◽  
Djoko Soeatmadji ◽  
Karyono Mintaroem
Keyword(s):  
Colorectal Cancer ◽  
Cell Line ◽  
Cell Lines ◽  
Early Stage ◽  
Hct116 Cell ◽  
Sw480 Cell ◽  
Control Group ◽  
Cancer Management ◽  
Sw480 Cell Line ◽  
E Cadherin

BACKGROUND: Early metastases is still unresolved problem in cancer management, eventually in colorectal cancer (CRC). In addition, many markers are useful just only in the late stage of CRC. AIM: This study evaluates the differences in the expression intensity of nuclear β-catenin, cytoplasmic β-catenin, E-cadherin, and N-cadherin between CRC SW480 cell line as control group and COLO320DM and HCT116 cell lines as case groups. MATERIALS AND METHODS: This study applied experimental research design with the different test methods. Culture growing and subcultures manufacturing for the CRC cell line models were done initially and followed by the immunofluorescence method by administering antibodies on β-catenin, E-cadherin, and N-cadherin, and continued with staining process using fluorescein-5-isothiocyanate and 4’, 6-diamidino-2-phenylindole. Observations were done using an immunofluorescence microscope. Calculation of area density in each cell to perceive the expressions of cytoplasmic and nuclear β-catenin, E-cadherin, and N-cadherin was conducted using ImageJ software, resulted in mean fluorescence intensity. RESULTS: There are significant differences in the expressions of cytoplasmic β-catenin, nuclear β-catenin, E-cadherin, and N-cadherin among SW480, COLO320DM, and HCT116 cell lines (p < 0.05). Despite no significant differences in cytoplasmic and nuclear β-catenin expressions between SW480 and HCT116 cell lines, and in E-cadherin and N-cadherin expressions between COLO320DM and HCT116 cell lines (p > 0.05). SW480 cell line has a higher expression of nuclear β-catenin than the cytoplasm (p < 0.05). CONCLUSION: This study reveals differences in the expression of nucleic and cytoplasmic β-catenin, E-cadherin, and N-cadherin in three stages of CRC (Duke B, C, and D) refer to different activation invasion, migration, and metastatic processes. Furthermore, the high expression of nuclear β-catenin and N-cadherin in the early stage of CRC indicate there is a metastatic process in that stage, so nuclear β-catenin and cadherin can be considered as potential biomarkers in the early stage of this cancer.

scholarly journals Mir-186-5p Regulates WNT Signaling Pathway by Targeting TCF4 Transcription Factor

2018 ◽  
Vol 8 (1) ◽  
pp. 130
Author(s):  
Zahra Bayat ◽  
Bahram M. Soltani
Keyword(s):  
Gene Expression ◽  
Cell Cycle ◽  
Wnt Signaling ◽  
Cell Line ◽  
Signaling Pathway ◽  
Wnt Pathway ◽  
Wnt Signaling Pathway ◽  
Sw480 Cell ◽  
Sw480 Cell Line ◽  
Sw480 Cells

The evolutionarily conserved Wnt signaling pathway plays essential roles during embryonic development, tissue homeostasis and differentiation. This pathway is deregulated in many cancers especially colorectal cancer. MiRNAs are a class of small noncoding RNAs that may play a major role in post transcriptional regulation of many genes and signaling pathway such as WNT signaling pathway. Here, we intended to investigate if miR-186-5p is capable of regulating WNT signaling pathway wia suppression TCF4 gene expression. miR-186-5p was bioinformatically predicted as a candidate regulator of TCF4 gene expression and then, in this experimental study, miR-186-5p was overexpressed in SW480 cell line and its increased expression was detected through quantitative reverse transcription polymerase chain reaction (RT-qPCR). The effect of miR-186-5p on WNT pathway was analysied with TOP/FOP flash assay in SW480 cell line. Finally, flow cytometery was used to inves tigate the effect of miR-186-5p overexpression on cell cycle progression in SW480 cell line. miR-186-5p was overexpressed in the SW480 cell line and its overexpression resulted in significant reduction of the TCF4 mRNA level. TOP/FOP flash assay, confirmed the negative effect of miR-186-5p on the Wnt pathway in SW480 cells. Finally, Overexpression of miR186-5p in SW480 cells resulted in cell cycle arrest in subG1 phase, detected by flow cytometry. Overall, accumulative results indi-cated that miR-186-5p by targeting TCF4 is potentially one of the regulators of the WNT signaling pathway.

Survival and Function of Fibroblasts Stored as Stock Cultures at a Non-freezing Temperature

1993 ◽  
Vol 21 (2) ◽  
pp. 206-209
Author(s):  
Anders H. G. Andrén ◽  
Anders P. Wieslander
Keyword(s):  
Cell Growth ◽  
Cell Line ◽  
Cell Lines ◽  
Fibroblast Cell ◽  
Freezing Temperature ◽  
Mouse Fibroblast ◽  
Toxic Response ◽  
Normal Manner ◽  
And Function

Cytotoxicity, measured as inhibition of cell growth of cultured cell lines, is a widely used method for testing the safety of biomaterials and chemicals. One major technical disadvantage with this method is the continuous routine maintenance of the cell lines. We decided to investigate the possibility of storing stock cultures of fibroblasts (L-929) in an ordinary refrigerator as a means of reducing the routine workload. Stock cultures of the mouse fibroblast cell line L-929 were prepared in plastic vials with Eagle's minimum essential medium. The vials were stored in a refrigerator at 4–10°C for periods of 7–31 days. The condition of the cells after storage was determined as cell viability, cell growth and the toxic response to acrylamide, measured as cell growth inhibition. We found that the L-929 cell line can be stored for 2–3, weeks with a viabilty > 90% and a cell growth of about 95%, compared to L-929 cells grown and subcultured in the normal manner. The results also show that the toxic response to acrylamide, using refrigerator stored L-929 cells, corresponds to that of control L-929 cells. We concluded that it is possible to store L-929 cells in a refrigerator for periods of up to 3 weeks and still use the cells for in vitro cytotoxic assays.

scholarly journals Tracking changes in adaptation to suspension growth for MDCK cells: cell growth correlates with levels of metabolites, enzymes and proteins

2021 ◽  
Vol 105 (5) ◽  
pp. 1861-1874
Author(s):  
Sabine Pech ◽  
Markus Rehberg ◽  
Robert Janke ◽  
Dirk Benndorf ◽  
Yvonne Genzel ◽  
...  
Keyword(s):  
Cell Growth ◽  
Cell Line ◽  
Mdck Cells ◽  
Suspension Cell ◽  
Regulatory Mechanisms ◽  
Adherent Cell ◽  
Holistic View ◽  
Cellular Processes ◽  
Viral Vaccine ◽  
Analytical Approaches

Abstract Adaptations of animal cells to growth in suspension culture concern in particular viral vaccine production, where very specific aspects of virus-host cell interaction need to be taken into account to achieve high cell specific yields and overall process productivity. So far, the complexity of alterations on the metabolism, enzyme, and proteome level required for adaptation is only poorly understood. In this study, for the first time, we combined several complex analytical approaches with the aim to track cellular changes on different levels and to unravel interconnections and correlations. Therefore, a Madin-Darby canine kidney (MDCK) suspension cell line, adapted earlier to growth in suspension, was cultivated in a 1-L bioreactor. Cell concentrations and cell volumes, extracellular metabolite concentrations, and intracellular enzyme activities were determined. The experimental data set was used as the input for a segregated growth model that was already applied to describe the growth dynamics of the parental adherent cell line. In addition, the cellular proteome was analyzed by liquid chromatography coupled to tandem mass spectrometry using a label-free protein quantification method to unravel altered cellular processes for the suspension and the adherent cell line. Four regulatory mechanisms were identified as a response of the adaptation of adherent MDCK cells to growth in suspension. These regulatory mechanisms were linked to the proteins caveolin, cadherin-1, and pirin. Combining cell, metabolite, enzyme, and protein measurements with mathematical modeling generated a more holistic view on cellular processes involved in the adaptation of an adherent cell line to suspension growth. Key points • Less and more efficient glucose utilization for suspension cell growth • Concerted alteration of metabolic enzyme activity and protein expression • Protein candidates to interfere glycolytic activity in MDCK cells

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