Developmental programming: Interaction between prenatal BPA and postnatal overfeeding on cardiac tissue gene expression in female sheep

AbstractThe stress response is adaptive and aims to guarantee survival. However, the persistence of a stressor can culminate in pathology. Catecholamines released as part of the stress response over activate beta adrenoceptors (β-AR) in the heart. Whether and how stress affects the expression of components of the intracellular environment in the heart is still, however, unknown. This paper used microarray to analyze the gene expression in the left ventricle wall of rats submitted to foot shock stress, treated or not treated with the selective β2-AR antagonist ICI118,551 (ICI), compared to those of non-stressed rats also treated or not with ICI, respectively. The main findings were that stress induces changes in gene expression in the heart and that β2-AR plays a role in this process. The vast majority of genes disregulated by stress were exclusive for only one of the comparisons, indicating that, in the same stressful situation, the profile of gene expression in the heart is substantially different when the β2-AR is active or when it is blocked. Stress induced alterations in the expression of such a large number of genes seems to be part of stress-induced adaptive mechanism.

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Endurance Training Regulates Expression of Some Angiogenesis-Related Genes in Cardiac Tissue of Experimentally Induced Diabetic Rats

Biomolecules ◽

10.3390/biom11040498 ◽

2021 ◽

Vol 11 (4) ◽

pp. 498

Author(s):

Mojdeh Khajehlandi ◽

Lotfali Bolboli ◽

Marefat Siahkuhian ◽

Mohammad Rami ◽

Mohammadreza Tabandeh ◽

...

Keyword(s):

Gene Expression ◽

Endurance Training ◽

Molecular Mechanisms ◽

Cardiac Tissue ◽

Critical Role ◽

Diabetic Rats ◽

Moderate Intensity ◽

Pcr Analysis ◽

Cardiac Tissues ◽

The Impact

Exercise can ameliorate cardiovascular dysfunctions in the diabetes condition, but its precise molecular mechanisms have not been entirely understood. The aim of the present study was to determine the impact of endurance training on expression of angiogenesis-related genes in cardiac tissue of diabetic rats. Thirty adults male Wistar rats were randomly divided into three groups (N = 10) including diabetic training (DT), sedentary diabetes (SD), and sedentary healthy (SH), in which diabetes was induced by a single dose of streptozotocin (50 mg/kg). Endurance training (ET) with moderate-intensity was performed on a motorized treadmill for six weeks. Training duration and treadmill speed were increased during five weeks, but they were kept constant at the final week, and slope was zero at all stages. Real-time polymerase chain reaction (RT-PCR) analysis was used to measure the expression of myocyte enhancer factor-2C (MEF2C), histone deacetylase-4 (HDAC4) and Calmodulin-dependent protein kinase II (CaMKII) in cardiac tissues of the rats. Our results demonstrated that six weeks of ET increased gene expression of MEF2C significantly (p < 0.05), and caused a significant reduction in HDAC4 and CaMKII gene expression in the DT rats compared to the SD rats (p < 0.05). We concluded that moderate-intensity ET could play a critical role in ameliorating cardiovascular dysfunction in a diabetes condition by regulating the expression of some angiogenesis-related genes in cardiac tissues.

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Differences in Muscle and Adipose Tissue Gene Expression and Cardio-Metabolic Risk Factors in the Members of Physical Activity Discordant Twin Pairs

PLoS ONE ◽

10.1371/journal.pone.0012609 ◽

2010 ◽

Vol 5 (9) ◽

pp. e12609 ◽

Cited By ~ 39

Author(s):

Tuija Leskinen ◽

Rita Rinnankoski-Tuikka ◽

Mirva Rintala ◽

Tuulikki Seppänen-Laakso ◽

Eija Pöllänen ◽

...

Keyword(s):

Gene Expression ◽

Physical Activity ◽

Risk Factors ◽

Adipose Tissue ◽

Metabolic Risk Factors ◽

Metabolic Risk ◽

Adipose Tissue Gene Expression ◽

Tissue Gene Expression ◽

Discordant Twin

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Central injection of oxytocin reduces food intake and affects hypothalamic and adipose tissue gene expression in chickens

Domestic Animal Endocrinology ◽

10.1016/j.domaniend.2018.10.005 ◽

2019 ◽

Vol 67 ◽

pp. 11-20

Author(s):

Betty R. McConn ◽

Anna Koskinen ◽

D. Michael Denbow ◽

Elizabeth R. Gilbert ◽

Paul B. Siegel ◽

...

Keyword(s):

Gene Expression ◽

Adipose Tissue ◽

Food Intake ◽

Adipose Tissue Gene Expression ◽

Tissue Gene Expression

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Adipose Tissue Gene Expression in the Context of Inflammation and Obesity

Phytochemicals ◽

10.1201/9781420005905.ch6 ◽

2006 ◽

pp. 83-95

Author(s):

Philip Kern

Keyword(s):

Gene Expression ◽

Adipose Tissue ◽

Adipose Tissue Gene Expression ◽

Tissue Gene Expression

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Endocrine disruptors affect developmental programming of HOX gene expression

Fertility and Sterility ◽

10.1016/j.fertnstert.2007.12.030 ◽

2008 ◽

Vol 89 (2) ◽

pp. e57-e58 ◽

Cited By ~ 24

Author(s):

Hugh S. Taylor

Keyword(s):

Gene Expression ◽

Endocrine Disruptors ◽

Developmental Programming ◽

Hox Gene

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The Cardiac Tissue-Restricted Homeobox Protein Csx/Nkx2.5 Physically Associates with the Zinc Finger Protein GATA4 and Cooperatively Activates Atrial Natriuretic Factor Gene Expression

Molecular and Cellular Biology ◽

10.1128/mcb.18.6.3120 ◽

1998 ◽

Vol 18 (6) ◽

pp. 3120-3129 ◽

Cited By ~ 212

Author(s):

Youngsook Lee ◽

Tetsuo Shioi ◽

Hideko Kasahara ◽

Shawn M. Jobe ◽

Russell J. Wiese ◽

...

Keyword(s):

Gene Expression ◽

Zinc Finger ◽

Protein Interaction ◽

Binding Sites ◽

Atrial Natriuretic Factor ◽

Target Gene ◽

Cardiac Tissue ◽

Zinc Finger Protein ◽

Finger Protein ◽

Homeobox Protein

ABSTRACT Specification and differentiation of the cardiac muscle lineage appear to require a combinatorial network of many factors. The cardiac muscle-restricted homeobox protein Csx/Nkx2.5 (Csx) is expressed in the precardiac mesoderm as well as the embryonic and adult heart. Targeted disruption of Csx causes embryonic lethality due to abnormal heart morphogenesis. The zinc finger transcription factor GATA4 is also expressed in the heart and has been shown to be essential for heart tube formation. GATA4 is known to activate many cardiac tissue-restricted genes. In this study, we tested whether Csx and GATA4 physically associate and cooperatively activate transcription of a target gene. Coimmunoprecipitation experiments demonstrate that Csx and GATA4 associate intracellularly. Interestingly, in vitro protein-protein interaction studies indicate that helix III of the homeodomain of Csx is required to interact with GATA4 and that the carboxy-terminal zinc finger of GATA4 is necessary to associate with Csx. Both regions are known to directly contact the cognate DNA sequences. The promoter-enhancer region of the atrial natriuretic factor (ANF) contains several putative Csx binding sites and consensus GATA4 binding sites. Transient-transfection assays indicate that Csx can activate ANF reporter gene expression to the same extent that GATA4 does in a DNA binding site-dependent manner. Coexpression of Csx and GATA4 synergistically activates ANF reporter gene expression. Mutational analyses suggest that this synergy requires both factors to fully retain their transcriptional activities, including the cofactor binding activity. These results demonstrate the first example of homeoprotein and zinc finger protein interaction in vertebrates to cooperatively regulate target gene expression. Such synergistic interaction among tissue-restricted transcription factors may be an important mechanism to reinforce tissue-specific developmental pathways.

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