Assessment of polycyclic aromatic hydrocarbon exposure, lung function, systemic inflammation, and genotoxicity in peripheral blood mononuclear cells from firefighters before and after a work shift

Abstract Background Schizophrenia is a severe, heritable, and refractory psychiatric disorder. Several studies have shown that the disrupted in schizophrenia 1 (DISC1) gene is closely associated with schizophrenia by its role in neuronal morphology, synaptic function, brain development, and dopamine homeostasis etc. This study intended to investigate the expression levels of DISC1 gene in schizophrenia patients compared with healthy controls, and the expression variation of DISC1 gene before and after antipsychotic treatment in schizophrenia patients. Methods In this study, we compared DISC1 expression levels in blood of 48 healthy controls, and 32 schizophrenia patients before and after 12 weeks of antipsychotic treatment using real-time quantitative PCR (RT-qPCR) analysis. Results The expression levels of DISC1 gene in peripheral blood mononuclear cells of schizophrenia patients before antipsychotic treatment were higher than those in healthy controls (P < 0.01); whereas after antipsychotic treatment, the expression levels of DISC1 gene in peripheral blood mononuclear cells of schizophrenia patients still remained increased (P < 0.01). Conclusions Our study provided further support for the involvement of DISC1 in the development of schizophrenia.

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Changes in Peripheral Blood Mononuclear Cells after Calving in Lactating Cows with Serious Decrease of Body Weight before and after Calving

Journal of Veterinary Medical Science ◽

10.1292/jvms.70.153 ◽

2008 ◽

Vol 70 (2) ◽

pp. 153-158 ◽

Cited By ~ 2

Author(s):

Hiromichi OHTSUKA ◽

Hitomi KASE ◽

Takaaki ANDO ◽

Masayuki KOHIRUIMAKI ◽

Machiko MASUI ◽

...

Keyword(s):

Body Weight ◽

Peripheral Blood Mononuclear Cells ◽

Peripheral Blood ◽

Mononuclear Cells ◽

Peripheral Blood Mononuclear ◽

Lactating Cows ◽

Blood Mononuclear Cells ◽

Before And After

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Determination of 2´-5´-Oligoadenylate Synthetase in Serum and Peripheral Blood Mononuclear Cells before and after Subcutaneous Application of Recombinant Interferon beta and gamma

Clinical Chemistry and Laboratory Medicine (CCLM) ◽

10.1515/cclm.1992.30.9.521 ◽

1992 ◽

Vol 30 (9) ◽

Author(s):

G. Bruchelt ◽

G. Fierlbeck ◽

Ursula Schiebel ◽

O. Bogenschütz ◽

G. Rassner ◽

...

Keyword(s):

Peripheral Blood Mononuclear Cells ◽

Peripheral Blood ◽

Interferon Beta ◽

Mononuclear Cells ◽

Oligoadenylate Synthetase ◽

Peripheral Blood Mononuclear ◽

Recombinant Interferon ◽

Blood Mononuclear Cells ◽

Before And After

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.GAMMA..DELTA. T Lymphocytes and Their V.GAMMA.9 and V.DELTA.2 Receptor Expression in Peripheral Blood Mononuclear Cells of Active Tuberculosis Patients Before and After Treatment.

The Kurume Medical Journal ◽

10.2739/kurumemedj.47.5 ◽

2000 ◽

Vol 47 (1) ◽

pp. 5-11

Author(s):

NAOKO SARUWATARI

Keyword(s):

Peripheral Blood Mononuclear Cells ◽

Peripheral Blood ◽

Mononuclear Cells ◽

Active Tuberculosis ◽

Receptor Expression ◽

Gamma Delta ◽

Peripheral Blood Mononuclear ◽

Tuberculosis Patients ◽

Blood Mononuclear Cells ◽

Before And After

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Detection of tumor cells in purged bone marrow and peripheral-blood mononuclear cells by polymerase chain reaction amplification of bcl-2 translocations.

Journal of Clinical Oncology ◽

10.1200/jco.1994.12.5.1021 ◽

1994 ◽

Vol 12 (5) ◽

pp. 1021-1027 ◽

Cited By ~ 31

Author(s):

R S Negrin ◽

J Pesando

Keyword(s):

Bone Marrow ◽

Polymerase Chain Reaction ◽

Tumor Cells ◽

Peripheral Blood Mononuclear Cells ◽

Mononuclear Cells ◽

Chain Reaction ◽

Peripheral Blood Mononuclear ◽

Blood Mononuclear Cells ◽

Before And After ◽

Polymerase Chain

PURPOSE To compare bone marrow (BM) before and after purging with monoclonal antibodies (MAbs) and complement with peripheral-blood mononuclear cells (PBMNCs) for tumor-cell contamination by amplification of t(14;18) sequences using the polymerase chain reaction (PCR). PATIENTS AND METHODS Sixty patients with non-Hodgkin's lymphoma (NHL) undergoing autologous BM transplantation were evaluated. Six BM biopsies were performed at the time of harvesting and evaluated morphologically for tumor involvement. The harvested BM was treated with a panel of anti-B-cell MAbs directed against CD9, CD10, CD19, and CD20, followed by rabbit complement. Clonogenic assays were performed before and after purging. DNA was extracted and t(14;18) sequences amplified by PCR. PBMNCs collected by apheresis for back-up purposes were similarly evaluated. RESULTS Fifteen patients (25%) were PCR-positive before BM purging. Following MAb- and complement-mediated purging, there was a reduction in the PCR-amplified signal in 10 patients (67%). There was no reduction in colony-forming unit granulocyte-macrophage (CFU-GM) colony growth following purging. Eight of these 15 patients (53%) had morphologic evidence of BM involvement at the time of harvesting. In these eight patients, only three had a reduction in the PCR-amplified products, as compared with all seven who were morphologically negative at the time of BM harvesting (P = .026). Fourteen of these 15 patients had PBMNCs collected near the time of BM harvesting and 12 (86%) were PCR-positive. CONCLUSION BM purging with MAbs and complement results in reduction in the number of t(14;18)-positive tumor cells, especially in those patients who have no morphologic evidence of BM disease at the time of harvesting. Purged BM was less contaminated with t(14;18)-positive cells than unpurged PBMNCs, which were frequently contaminated with tumor cells.

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SIRT1 Activity in Peripheral Blood Mononuclear Cells Correlates with Altered Lung Function in Patients with Chronic Obstructive Pulmonary Disease

Oxidative Medicine and Cellular Longevity ◽

10.1155/2018/9391261 ◽

2018 ◽

Vol 2018 ◽

pp. 1-8 ◽

Cited By ~ 7

Author(s):

Valeria Conti ◽

Graziamaria Corbi ◽

Valentina Manzo ◽

Paola Malangone ◽

Carolina Vitale ◽

...

Keyword(s):

Oxidative Stress ◽

Chronic Obstructive Pulmonary Disease ◽

Lung Function ◽

Peripheral Blood Mononuclear Cells ◽

Mononuclear Cells ◽

Chronic Obstructive ◽

Peripheral Blood Mononuclear ◽

Obstructive Pulmonary Disease ◽

Copd Patients ◽

Blood Mononuclear Cells

Background. Oxidative stress is a recognized pathogenic mechanism in chronic obstructive pulmonary disease (COPD). Expression of the NAD+-dependent deacetylase Sirtuin 1 (SIRT1), an antiaging molecule with a key role in oxidative stress response, has been described as decreased in the lung of COPD patients. No studies so far investigated whether systemic SIRT1 activity was associated to decreased lung function in this disease. Methods. We measured SIRT1 protein expression and activity in peripheral blood mononuclear cells (PBMCs) and total oxidative status (TOS), total antioxidant capacity (TEAC), and oxidative stress index (TOS/TEAC) in the plasma of 25 COPD patients, 20 healthy nonsmokers (HnS), and 20 healthy smokers (HS). Results. The activity of SIRT1 was significantly lower in COPD patients compared to both control groups while protein expression decreased progressively (HnS > HS > COPD). TOS levels were significantly lower in HnS than in smoke-associated subjects (COPD and HS), while TEAC levels were progressively lower according (HnS > HS > COPD). In COPD patients, SIRT1 activity, but not protein levels, correlated significantly with both lung function parameters (FEV1/FVC and FEV1) and TEAC. Conclusions. These findings suggest loss of SIRT1-driven antioxidant activity as relevant in COPD pathogenesis and identify SIRT1 activity as a potential convenient biomarker for identification of mild/moderate, stable COPD.

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