Binding of 3-hydroxybenzo[a]pyrene to bovine hemoglobin and albumin

2003 ◽  
Vol 17 (4) ◽  
pp. 239-247 ◽  
Author(s):  
Narumi Sugihara ◽  
Margaret O. James
Keyword(s):  
Bovine Hemoglobin

Studies on Chromatographic Fractioning by Cations Exchangers of a Bovine Hemoglobin Hydrolysate

2018 ◽  
Vol 69 (10) ◽  
pp. 2794-2798
Author(s):  
Alina Diana Panainte ◽  
Ionela Daniela Morariu ◽  
Nela Bibire ◽  
Madalina Vieriu ◽  
Gladiola Tantaru ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Amino Acids ◽  
Retention Time ◽  
Chromatographic Separation ◽  
Bovine Hemoglobin ◽  
Reverse Phase ◽  
Hplc System ◽  
Fast Flow ◽  
Hydrolysis Of ◽  
Phase Column

A peptidic hydrolysate has been obtained through hydrolysis of bovine hemoglobin using pepsin. The fractioning of the hydrolysate was performed on a column packed with CM-Sepharose Fast Flow. The hydrolysate and each fraction was filtered and then injected into a HPLC system equipped with a Vydak C4 reverse phase column (0.46 x 25 cm), suitable for the chromatographic separation of large peptides with 20 to 30 amino acids. The detection was done using mass spectrometry, and the retention time, size and distribution of the peptides were determined.

Anionic Binding Site and 2,3-DPG Effect in Bovine Hemoglobin†

Biochemistry ◽  
1998 ◽  
Vol 37 (40) ◽  
pp. 14024-14029 ◽  
Author(s):  
M. Marta ◽  
M. Patamia ◽  
A. Colella ◽  
S. Sacchi ◽  
M. Pomponi ◽  
...  
Keyword(s):  
Binding Site ◽  
Bovine Hemoglobin ◽  
2,3 Dpg

Preparation of carbon-based metal organic frameworks modified molecularly imprinted polymers for selective recognition of bovine hemoglobin in biological samples

2022 ◽  
Author(s):  
Xue Chen ◽  
Jinyue Chai ◽  
Baodong Sun ◽  
Xue Yang ◽  
Feng Zhang ◽  
...  
Keyword(s):  
Molecularly Imprinted Polymers ◽  
Metal Organic Framework ◽  
Selective Recognition ◽  
Template Molecule ◽  
Bovine Hemoglobin ◽  
Carbon Spheres ◽  
Imprinted Polymer ◽  
Molecularly Imprinted ◽  
Metal Organic ◽  
Carbon Based

In this study, the carbon-based Cu2+-immobilized metal-organic framework modified molecularly imprinted polymer (C@GI@Cu-MOFs@MIPs) adsorbent was prepared using bovine hemoglobin (BHb) as a template molecule with carbon spheres as carriers for...

scholarly journals The interaction of clenbuterol hydrochloride with bovine hemoglobin using spectroscopic techniques and molecular modeling methods

2009 ◽  
Vol 23 (5-6) ◽  
pp. 271-279 ◽  
Author(s):  
Yun Wu ◽  
Hui Mao ◽  
Bo Zhao ◽  
Jian Shen
Keyword(s):  
Molecular Modeling ◽  
Protein Secondary Structure ◽  
Binding Mode ◽  
Bovine Hemoglobin ◽  
Spectroscopic Techniques ◽  
Electronic Interaction ◽  
Physiological Conditions ◽  
Modeling Methods ◽  
Clenbuterol Hydrochloride ◽  
Hoff Equation

The interaction of clenbuterol hydrochloride (CL) to bovine hemoglobin (BHb) under physiological conditions was investigated by using UV-vis absorption, fluorescence, circular dichroism (CD) and molecular modeling. The fluorescence intensity of BHb decreased regularly with the gradual increasing concentration of CL. It is observed that there was a prominent interaction between CL and BHb. The fluorescence data revealed that the fluorescence quenching is a static process, and the thermodynamic parameters were calculated according to the Van't Hoff equation. The alternations of protein secondary structure in the presence of CL were determined by the evidence of CD. Molecular modeling study that corroborate our experimental results revealed that the binding mode of CL–BHb complex could be attributed to the hydrophobic interaction and hydrogen bonding, but electronic interaction cannot be excluded.

Preservation of myocardial energy status by bovine hemoglobin solutions during ischemia

2002 ◽  
Vol 56 (5) ◽  
pp. 247-253 ◽  
Author(s):  
F. Carlucci ◽  
F. Miraldi ◽  
A. Barretta ◽  
A.G.M. Marullo ◽  
E. Marinello ◽  
...  
Keyword(s):  
Bovine Hemoglobin ◽  
Energy Status
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