Enhanced Entrapment and Improved in Vitro Controlled Release of N-Acetyl Cysteine in Hybrid PLGA/Lecithin Nanoparticles Prepared Using a Nanoprecipitation/Self-Assembly Method

Background: Nowadays investigations in the field of dental implants engineering are focused on bioactivity and osseointegration properties.Objective: In this study, the oxide-covered titanium was functionalized by vitamin D3 molecules via a simple self-assembly method with the aim to design more corrosion resistant and at the same time more bioactive surface.Methods: Surface properties of the D3-coated titanium were examined by scanning electron microscopy, attenuated total reflectance Fourier transform infrared spectroscopy, and contact angle measurements, while a long-term corrosion stability during immersion in an artificial saliva solution was investigated in situ by electrochemical impedance spectroscopy.Results: Results of all techniques confirmed a successful formation of the D3 vitamin layer on the oxide-covered titanium. Besides very good corrosion resistivity (~5 MΩcm2 ) the D3-modified titanium surface induced spontaneous formation of biocompatible bone-like calcium phosphates (CaP).Conclusion: Observed in vitro CaP-forming ability as a result of D3-modified titanium/artificial saliva interactions could serve as a promising predictor of in vivo bioactivity of implant materials.

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Human Organ-Specific 3D Cancer Models Produced by the Stromal Self-Assembly Method of Tissue Engineering for the Study of Solid Tumors

BioMed Research International ◽

10.1155/2020/6051210 ◽

2020 ◽

Vol 2020 ◽

pp. 1-23 ◽

Cited By ~ 1

Author(s):

Vincent Roy ◽

Brice Magne ◽

Maude Vaillancourt-Audet ◽

Mathieu Blais ◽

Stéphane Chabaud ◽

...

Keyword(s):

Tissue Engineering ◽

Tumor Microenvironment ◽

Self Assembly ◽

The Self ◽

Human Organ ◽

Assembly Method ◽

Cancer Models ◽

Organ Specific

Cancer research has considerably progressed with the improvement of in vitro study models, helping to understand the key role of the tumor microenvironment in cancer development and progression. Over the last few years, complex 3D human cell culture systems have gained much popularity over in vivo models, as they accurately mimic the tumor microenvironment and allow high-throughput drug screening. Of particular interest, in vitrohuman 3D tissue constructs, produced by the self-assembly method of tissue engineering, have been successfully used to model the tumor microenvironment and now represent a very promising approach to further develop diverse cancer models. In this review, we describe the importance of the tumor microenvironment and present the existing in vitro cancer models generated through the self-assembly method of tissue engineering. Lastly, we highlight the relevance of this approach to mimic various and complex tumors, including basal cell carcinoma, cutaneous neurofibroma, skin melanoma, bladder cancer, and uveal melanoma.

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Engineering Tissues without the Use of a Synthetic Scaffold: A Twenty-Year History of the Self-Assembly Method

BioMed Research International ◽

10.1155/2018/5684679 ◽

2018 ◽

Vol 2018 ◽

pp. 1-13 ◽

Cited By ~ 5

Author(s):

Ingrid Saba ◽

Weronika Jakubowska ◽

Stéphane Bolduc ◽

Stéphane Chabaud

Keyword(s):

Extracellular Matrix ◽

Self Assembly ◽

Large Scale ◽

Dermal Fibroblasts ◽

The Self ◽

Production Costs ◽

Scale Production ◽

Assembly Method ◽

Assembly Technique

Twenty years ago, Dr. François A. Auger, the founder of the Laboratory of Experimental Organogenesis (LOEX), introduced the self-assembly technique. This innovative technique relies on the ability of dermal fibroblasts to produce and assemble their own extracellular matrix, differing from all other tissue-engineering techniques that use preformed synthetic scaffolds. Nevertheless, the use of the self-assembly technique was limited for a long time due to its main drawbacks: time and cost. Recent scientific breakthroughs have addressed these limitations. New protocol modifications that aim at increasing the rate of extracellular matrix formation have been proposed to reduce the production costs and laboratory handling time of engineered tissues. Moreover, the introduction of vascularization strategies in vitro permits the formation of capillary-like networks within reconstructed tissues. These optimization strategies enable the large-scale production of inexpensive native-like substitutes using the self-assembly technique. These substitutes can be used to reconstruct three-dimensional models free of exogenous materials for clinical and fundamental applications.

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Self-Assembly Iron Oxide Nanoclusters for Photothermal-Mediated Synergistic Chemo/Chemodynamic Therapy

Journal of Immunology Research ◽

10.1155/2021/9958239 ◽

2021 ◽

Vol 2021 ◽

pp. 1-10

Author(s):

Xiang Li ◽

Zhen Wang ◽

Mian Ma ◽

Zhouqing Chen ◽

Xiang-long Tang ◽

...

Keyword(s):

Iron Oxide ◽

Self Assembly ◽

Near Infrared ◽

Light Irradiation ◽

Infrared Light ◽

Photothermal Effect ◽

Photothermal Conversion ◽

Assembly Method

Background and Purpose. Although chemodynamic therapy (CDT) is promising for cancer treatment, its clinical application is still limited because of unresolved issues. In this study, an efficient CDT agent for synergistic chemo/CDT therapy mediated by the photothermal effect was developed by an iron oxide self-assembly method. Methods. Superparamagnetic iron oxide nanoclusters (SPIOCs) were located within the core, which resulted in high photothermal conversion and outstanding generation of reactive oxygen species (ROS). The shell consisted of a human serum albumin- (HSA-) paclitaxel (PTX) layer, which extended the blood circulation time and ensured the effectiveness of the chemotherapy. Arg-Gly-Asp peptides (RGD) were linked to the naked cysteine moieties in HSA to promote the specific targeting of human glioma U87 cells by αvβ3 integrins. Continuous near-infrared light irradiation triggered and promoted the synergistic chemo/CDT therapy through the photothermal effect. Results. Our SPIOCs@HSA-RGD nanoplatform showed well biocompatibility and could target glioma specifically. Photothermal conversion and ROS burst were detected after continuous 808 nm light irradiation, and a significant antitumor effect was achieved. Conclusion. Experimental in vitro and in vivo evaluations showed that our photothermal-mediated chemo/CDT therapy could efficiently inhibit tumor growth and is therefore promising for cancer therapy.

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Paclitaxel-tyroserleutide Conjugates Self-assembly into Nanocarrier for Drug Delivery

Letters in Drug Design & Discovery ◽

10.2174/1570180815666180803124625 ◽

2019 ◽

Vol 16 (8) ◽

pp. 882-891

Author(s):

Yongjia Liu ◽

Leilei Shi ◽

Bangshang Zhu ◽

Yue Su ◽

Hui Li ◽

...

Keyword(s):

Drug Delivery ◽

Self Assembly ◽

Water Solubility ◽

Drug Carrier ◽

Drug Loading ◽

Cancer Therapeutics ◽

Drug Carriers ◽

Pharmacokinetic Studies ◽

Assembly Method

Background: The drug-drug self-assembly was considered as a simple and efficient approach to prepare high drug loading nano-drug carriers and present new opportunities for cancer therapeutics. The strategy of PTX amphiphiles preparation would be a possible way to solve the poor water solubility of PTX. Methods: The PTX-YSL conjugate were synthesized and characterized. The PTX-YSL nanocarriers was prepared by a simple self-assembly method. In vitro cell studies and pharmacokinetic studies were evaluated for their in vitro anti-tumor activities and blood retention time. Results: The structures of PTX-YSL conjugate were confirmed by LC-MS, 1H NMR and FTIR. The size and morphology of the PTX-YSL self-assembled nanocarriers were observed with TEM and DLS. PTX-YSL nanocarriers could facilitate cellular uptake and had low cytotoxicity. PTX-YSL nanocarriers have longer blood retention for enhancing accumulation in the tumor tissues via EPR effect. Conclusion: This drug delivery system formed by PTX-YSL conjugates constitutes a promising and effective drug carrier in cancer therapy.

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Cysteine and N-acetyl cysteine encapsulated mesoporous silica: synthesis, characterization and influence of parameters on in-vitro controlled release

Journal of Porous Materials ◽

10.1007/s10934-016-0351-4 ◽

2017 ◽

Vol 24 (4) ◽

pp. 1105-1115

Author(s):

Soyeb Pathan ◽

Priyanka Solanki ◽

Anjali Patel

Keyword(s):

Controlled Release ◽

Mesoporous Silica ◽

Acetyl Cysteine ◽

Silica Synthesis

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Intercalation of Fenoprofen into Layered Double Hydroxide for the Formation of Controlled Release Anti-Inflammatory Drug

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.1142.230 ◽

2017 ◽

Vol 1142 ◽

pp. 230-233 ◽

Cited By ~ 1

Author(s):

Siti Halimah Sarijo ◽

Monica Limau Jadam ◽

Faizah Md Saleh

Keyword(s):

Controlled Release ◽

Organic Compound ◽

Layered Double Hydroxide ◽

Self Assembly ◽

Interlayer Spacing ◽

Nanocomposite Materials ◽

Molar Ratio ◽

Hybrid Nanocomposite ◽

Assembly Method ◽

Double Hydroxide

Synthesis of a new hybrid nanocomposite materials, zinc-aluminium-fenoprofen, ZAF was successfully accomplished by self-assembly method with molar ratio of Zn to Al; R=2 at concentration of fenoprofen=0.3 M. As a result of the successful intercalation, the interlayer spacing expanded from 9.8 Å in the zinc-aluminium-layered double hydroxide, ZAL to 20.1 Å in the ZAF hybrid nanocomposite. The FTIR spectra of the ZAF show resemblance peaks of ZAL and fenoprofen indicating the inclusion of the organic compound into the LDH interlamellae. The percentage loading of the guest anion, fenoprofen in ZAL was estimated to be about 63.4% (w/w) calculated from the carbon content.

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Polyphenols-Loaded Sericin Self-Assembling Nanoparticles: A Slow-Release for Regeneration by Tissue-Resident Mesenchymal Stem/Stromal Cells

Pharmaceutics ◽

10.3390/pharmaceutics12040381 ◽

2020 ◽

Vol 12 (4) ◽

pp. 381 ◽

Cited By ~ 1

Author(s):

Giulia Orlandi ◽

Elia Bari ◽

Laura Catenacci ◽

Milena Sorrenti ◽

Lorena Segale ◽

...

Keyword(s):

Oxidative Stress ◽

Stromal Cells ◽

Self Assembly ◽

Slow Release ◽

Biological Activities ◽

Epigallocatechin Gallate ◽

Burst Release ◽

Self Assembling ◽

Assembly Method

Mesenchymal stem/stromal cells (MSCs) are a therapeutic target to promote tissue regeneration, mainly when oxidative stress-mediated damage is involved in disease pathogenesis. Here, slow-release silk sericin nanoparticles (SNPs) loaded with natural antioxidant polyphenols were developed to sustain regeneration by tissue-resident MSCs. SNPs were prepared by exploiting a self-assembly method with poloxamer and were loaded with proanthocyanidins (P), quercetin (Q) or epigallocatechin gallate (E). SNPs, with a diameter less than 150 nm, were able to encapsulate both hydrophilic (P and E) and hydrophobic (Q) drugs. A slow and controlled release was obtained from SNPs for all the actives in PBS, while in EtOH, Q and E showed a burst release but P did not. Kinetic models revealed lower diffusion of P than other biomolecules, probably due to the higher steric hindrance of P. The in vitro anti-oxidant, anti-elastase and anti-tyrosinase properties of SNPs were assessed: loading the P and E into SNPs preserved the in vitro biological activities whereas for Q, the anti-elastase activity was strongly improved. Moreover, all formulations promoted MSC metabolic activity over 72 h. Finally, SNPs exhibited a strong ability to protect MSCs from oxidative stress, which supports their potential use for regenerative purposes mediated by tissue-resident MSCs.

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Preparation of Folate-Conjugated Pluronic F127/Chitosan Core-Shell Nanoparticles Encapsulating Doxorubicin for Breast Cancer Treatment

Journal of Nanomaterials ◽

10.1155/2012/593878 ◽

2012 ◽

Vol 2012 ◽

pp. 1-11 ◽

Cited By ~ 44

Author(s):

Chawan Manaspon ◽

Kwanchanok Viravaidya-Pasuwat ◽

Nuttaporn Pimpha

Keyword(s):

Self Assembly ◽

Sodium Dodecyl ◽

Pluronic F127 ◽

Core Shell ◽

Burst Release ◽

Shell Nanoparticles ◽

The Core ◽

Assembly Method ◽

Core Shell Nanoparticles

A targeting drug delivery system using folate-conjugated pluronic F127/chitosan core-shell nanoparticles was developed to deliver doxorubicin (DOX) to the target cancer cells. First, DOX was encapsulated in pluronic F127 micelle cores in the presence of sodium dodecyl sulfate (SDS) by a self-assembly method. To form a shell, a layer of either chitosan or folate-conjugated chitosan was deposited onto the pluronic micelles. The encapsulation efficiency was approximately58.1±4.7%. The average size of the core-shell nanoparticles was37.4±2.0 nm, while the zeta potential was12.9±2.3 mV, indicating the presence of a shell layer and more stable particles. In anin vitroDOX release study, an initial burst release, followed by a sustained release, was observed within 24 hours. In addition, the core-shell nanoparticles showed greater cytotoxicity towards MCF-7 cells than free DOX, suggesting a better therapeutic efficacy in treating cancer.

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