Effect of nitrogen concentration, two‐stage and prolonged cultivation on growth rate, lipid and protein content ofChlorella vulgaris

2019 ◽  
Vol 94 (5) ◽  
pp. 1466-1473 ◽  
Author(s):  
Maria N Metsoviti ◽  
Nikolaos Katsoulas ◽  
Ioannis T Karapanagiotidis ◽  
Georgios Papapolymerou
Keyword(s):  
Growth Rate ◽  
Protein Content ◽  
Nitrogen Concentration ◽  
Two Stage ◽  
Prolonged Cultivation

Toxic Effects of Quinalphos on Protein Content and Growth Rate in Freshwater Teleost, Cyprinus carpio (L.)

2016 ◽  
Vol 23 (1) ◽  
pp. 89
Author(s):  
A. Padmanabha ◽  
H. R. V. Reddy ◽  
N. B. Shridhar ◽  
Muttappa Khavi ◽  
B. T. Naveen Kumar
Keyword(s):  
Growth Rate ◽  
Protein Content ◽  
Cyprinus Carpio ◽  
Toxic Effects ◽  
Freshwater Teleost

scholarly journals Biological Impact of Photoperiod on Fairy Shrimp (Branchinecta orientalis): Life History and Biochemical Composition

Biology ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 695
Author(s):  
Sara Farhadi ◽  
Behrooz Atashbar Kangarloei ◽  
Ahmad Imani ◽  
Kourosh Sarvi Moghanlou
Keyword(s):  
Growth Rate ◽  
Life History ◽  
Protein Content ◽  
Biochemical Composition ◽  
Digestive Enzyme ◽  
Total Body Length ◽  
Constant Darkness ◽  
Fairy Shrimp ◽  
Daily Growth ◽  
Isoleucine Leucine

B. orientalis, fairy shrimp, is often among the most conspicuous invertebrates inhabiting temporary aquatic habitats with a typical variation in environmental conditions. Its life history characteristics and biochemical composition were studied under four different photoperiodic regimes (24L:0D, 0L:24D, 16L:8D, and 12L:12D). The significantly highest cumulative and initial hatching rates (48 h) were obtained at 24L:0D (p < 0.05). Cultivating the larvae under different photoperiods did not significantly affect specific growth rate (SGR) (p > 0.05). However, higher final total body length and daily growth rate were recorded under constant darkness. Higher lipid content was found at 24L:0D to the extent that it was more than two times higher than that at 16L:8D and 12L:12D (p < 0.05). There was also a remarkable increase in body crude protein content at 24L:0D (p < 0.05). Body fatty-acid profiles of the fairy shrimps were also affected by culture condition (p < 0.05). Extension of lighting period resulted in a subtle increase in body contents of arginine, lysine, histidine, isoleucine, leucine, valine, methionine, and phenylalanine, especially in the group kept under a 16L:8D regime. The highest and lowest digestive enzyme activity was observed at 0L:24D and 24L:0D, respectively (p < 0.05). In contrast, the highest and lowest soluble protein content was recorded at 24L:0D and 0L:24D, respectively (p < 0.05). Similarly, antioxidant status was significantly higher at 0L:24D (p < 0.05). In conclusion, a 16L:8D light–dark cycle might be an optimal condition in terms of growth performance and physio-biochemical characteristics. These findings could be helpful in optimizing the rearing conditions for upscaling B. orientalis production.

scholarly journals The ameliorative effect of selenium on Azolla caroliniana grown under UV-B stress

2015 ◽  
Vol 95 (1) ◽  
pp. 20-26 ◽  
Author(s):  
E.M. Mostafa ◽  
A.M.A. Hassan
Keyword(s):  
Growth Rate ◽  
Protein Content ◽  
Doubling Time ◽  
The Other ◽  
Total Glutathione ◽  
Nutrient Media ◽  
Relative Growth ◽  
Other Hand ◽  
Ameliorative Effect ◽  
Glutathione Redox

Exposure ofAzollaplants to UV-B radiation for 6 h resulted in a decrease in biomass and relative growth rate (RGR), which coincided with an increase in doubling time (DT) as compared with the control. Also, the protein content decreased. On the other hand, hydrogen peroxyde (H2O2) and malondialdehyde (MDA) accumulated significantly in UV-treatedAzollaplants. Conversely, the addition of selenium (Se) at 1 ppm resulted in a significant increase in biomass and protein content of untreated and UV-treatedAzollaplants, and a significant reduction in both H2O2and MDA. Moreover, the addition of Se to UV-treated and untreatedAzollaplants resulted in a significant increase in total ascorbate and total glutathione (GSH) contents compared with the control and UV-stressedAzollaplants. Also, glutathione redox potential (GSH/TG) increased significantly in UV-treatedAzollaplants in the presence of Se. There also was a significant increase (38%) in ascorbate peroxidase (APX) activity in UV-treated plants compared with the control. APX activity in the presence of Se did not change significantly compared with the control. Glutathione reductase (GR) activity increased significantly in UV-treatedAzolla, while glutathione peroxidase (GSH-PX) activity did not. On the other hand, both GSH-PX and GR activity in untreated and UV-treatedAzollaplants were significantly enhanced by the application of Se to the nutrient media at a concentration of 1 ppm. Therefore, we can conclude that Se protectsAzollaplants from UV-B stress.

Bioprocess optimisation for high cell density endoinulinase production from recombinant Aspergillus niger

2021 ◽  
Author(s):  
Pfariso Maumela ◽  
Shaunita Rose ◽  
Eugene van Rensburg ◽  
Annie Chimphango ◽  
Johann Gorgens
Keyword(s):  
Growth Rate ◽  
Aspergillus Niger ◽  
Cell Density ◽  
Enzyme Production ◽  
High Cell Density ◽  
Nitrogen Concentration ◽  
High Cell ◽  
Feed Concentration ◽  
Volumetric Activity ◽  
Activity Increase

Abstract Endoinulinases gene was expressed in recombinant Aspergillus niger for selective and high-level expression using an exponential fed-batch fermentation. The effects of the growth rate (µ), glucose feed concentration, nitrogen concentration and fungal morphology, on enzyme production were evaluated. A recombinant endoinulinases with a molecular weight of 66 KDa was secreted. Endoinulinases production was growth associated at µ> 0.04 h -1 , which is characteristic of the constitutive gpd promoter used for the enzyme production. The highest volumetric activity (670 U/ml) was achieved at a growth rate of 93% of µ max (0.07 h -1 ), while enzyme activity (506 U/ml) and biomass substrate yield (0.043 g biomassDW /g glucose ) significantly decreased at low µ (0.04 h -1 ). Increasing the feed concentration resulted in high biomass concentrations and viscosity, which necessitated high agitation for improved mixing and oxygen. However, the high agitation and low DO levels (ca. 8% of saturation) led to pellet disruption and growth in mycelial morphology. Enzyme production profiles, product (Y p/s ) and biomass (Y x/s ) yield coefficients were not affected by feed concentration and morphological change. The gradual increase in the concentration of nitrogen sources showed that, a nitrogen limited culture was not suitable for endoinulinases production in recombinant A. niger. Moreover, the increase in enzyme volumetric activity was still directly related to an increase in biomass concentration. An increase in nitrogen concentration, from 3.8 to 12 g/L, resulted in volumetric activity increase from 393 to 670 U/ml, but the Y p/s (10053 U/g glucose ) and Y x/s (0.049 g biomasDWs /g glucose ) did not significantly change. The data demonstrated the potential of recombinant A. niger and high cell density fermentation for the development of largescale endoinulinases production system.

scholarly journals EVALUATION OF ARTHROSPIRA SP. GROWTH ABILITY ON HEAVY METAL SALTS AND THEIR EFFECT ON SOME CELLULAR COMPONENTS

2020 ◽  
Vol 17 (34) ◽  
pp. 667-677
Author(s):  
Hiba Khaleel Saeed AL-SHAKARCHI ◽  
Yousef Jabbar AL-SHAHERY
Keyword(s):  
Growth Rate ◽  
Protein Content ◽  
Biomass Concentration ◽  
Local Strain ◽  
Food Supplement ◽  
Metal Salts ◽  
Chlorophyll Contents ◽  
Copper Nickel ◽  
Iron Copper ◽  
Nickel Cadmium

rthrospira sp. is an aquatic and photosynthetic microorganism that is extensively employed as a food supplement due to its rich contents of nutrients, proteins, and carbohydrates. In this study, a local strain of cyanobacterium of the genus Arthrospira was isolated from the Iraqi soil, in the region of Mosul city, using the Chu’s Medium No. 10. The growth rate, as well as the effects on biomass and cellular component contents of proteins, carbohydrates, and chlorophyll of this strain, were evaluated on the molasses medium alone or supplemented with iron, copper, nickel, cadmium and cobalt salts after fifteen days of incubation. The results showed that the best growth rate (1.09 OD), the highest value of biomass (120.0 mg/l), proteins content (297.2 mg/l), chlorophyll content (14.9 mg/l) and carbohydrates content (400.0 mg/l) of Arthrospira sp. was achieved after fifteen days of incubation. Generally, it was observed that adding iron, copper, nickel, cadmium, and cobalt salts into the molasses medium increased the contents of biomass, proteins, and carbohydrates of Arthrospira sp.. It was noticed that the highest biomass concentration (1960 mg/l) was obtained when Arthrospira sp. grown on molasses medium supplemented with nickel. Also, none of the metal salts added to the molasses medium increased the protein content of Arthrospira sp.. Conversely, adding copper, nickel, and cobalt to the medium showed an adverse effect on the protein content. It was shown that adding iron metal salts into the molasses medium increased the carbohydrates and the chlorophyll contents of Arthrospira sp.. These results suggest that Arthrospira sp. can be utilized for the bioremediation of heavy metals pollution in the environment and industrial sites.

Effect of dietary protein content on growth rate, survival and body composition of juvenile cauque river prawn,Macrobrachium americanum(Bate 1868)

2016 ◽  
Vol 48 (3) ◽  
pp. 741-751 ◽  
Author(s):  
Yuniel Méndez-Martínez ◽  
Stig Yamasaki-Granados ◽  
Marcelo U García-Guerrero ◽  
Luis R Martínez-Córdova ◽  
Marta E Rivas-Vega ◽  
...  
Keyword(s):  
Growth Rate ◽  
Body Composition ◽  
Protein Content ◽  
Dietary Protein

Effect of Two-Stage Aging on Microstructure and Properties of Al-Mg-Si Alloys

2022 ◽  
Vol 905 ◽  
pp. 51-55
Author(s):  
Li Wang ◽  
Ya Ya Zheng ◽  
Shi Hu Hu
Keyword(s):  
Growth Rate ◽  
Grain Boundary ◽  
Tensile Properties ◽  
Tensile Testing ◽  
Diffusion Rate ◽  
Two Stage ◽  
Precipitated Phase ◽  
Transmission Electron ◽  
The Matrix ◽  
Microstructure Characteristic

The effects of two-stage aging on the microstructures, tensile properties and intergranular corrosion (IGC) sensitivity of Al-Mg-Si alloys were studied by tensile testing and IGC experiments and transmission electron microscope (TEM). The results show that the two-stage aging (180°C, 2h+160°C, 120h) can reduce the IGC sensitivity without decrease the tensile properties. The grain is distributed with high-density β′′ phases, and the grain boundary phases are spherical and intermittently distributed. The formation of the microstructure characteristic is due to the lower re-aging temperature, which results in a decline differences in the diffusion rate between the matrix and grain boundaries. As a result, the pre-precipitated phase can maintain a better strengthening effects due to the slower growth rate. The pre-precipitated phase of the grain boundary presents a spherical and intermittent distribution due to the fast coarsening speed.

A note on the effect of amino acid or urea supplements to barley diets for the finishing pig

1969 ◽  
Vol 11 (4) ◽  
pp. 553-556 ◽  
Author(s):  
H. F. Hintz ◽  
W. G. Pond ◽  
W. J. Visek
Keyword(s):  
Growth Rate ◽  
Amino Acid ◽  
Protein Content ◽  
Cottonseed Meal ◽  
Carcass Characteristics ◽  
Basal Diet ◽  
Soya Bean ◽  
Finishing Pig ◽  
Bean Meal ◽  
Soya Bean Meal

SUMMARYIn trials 1 and 2, supplements of urea and cottonseed meal increased the protein content of the basal diets from 12·2 to 14·0–14·6%, but had no significant effects on growth rate or carcass characteristics. In trial 3 the effect of cottonseed meal on growth rate was almost significant but urea had less effect. In trial 4, the basal diet contained 11·0% protein; soya bean meal increased this to 13·1% and had an almost significant effect on growth rate. Lysine and lysine + urea had no effect.

Medium N:P Ratios and Specific Growth Rate ComodulateMicrocystin and Protein Content in Microcystis aeruginosa PCC7806 and M. aeruginosa UV027

2005 ◽  
Vol 49 (3) ◽  
pp. 468-473 ◽  
Author(s):  
T.G. Downing ◽  
C.S. Sember ◽  
M.M. Gehringer ◽  
W. Leukes
Keyword(s):  
Growth Rate ◽  
Specific Growth Rate ◽  
Protein Content ◽  
Microcystis Aeruginosa ◽  
Specific Growth ◽  
N:P Ratios
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