Protective Effects of Topical Application of a Poorly Soluble Antioxidant Astaxanthin Liposomal Formulation on Ultraviolet-Induced Skin Damage

AbstractThe present study aimed to evaluate the protective effect of a methanol extract of Sargassum horneri (SHM), which contains 6-hydroxy-4,4,7a-trimethyl-5,6,7,7a-tetrahydrobenzofuran-2(4H)-one (HTT) and apo-9′-fucoxanthinone, against ultraviolet B (UVB)-induced cellular damage in human keratinocytes and its underlying mechanism. SHM significantly improved cell viability of UVB-exposed human keratinocytes by reducing the generation of intracellular reactive oxygen species (ROS). Moreover, SHM inhibited UVB exposure-induced apoptosis by reducing the formation of apoptotic bodies and the populations of the sub-G1 hypodiploid cells and the early apoptotic cells by modulating the expression of the anti- and pro-apoptotic molecules, Bcl-2 and Bax, respectively. Furthermore, SHM inhibited NF-κB p65 activation by inducing the activation of Nrf2/HO-1 signaling. The cytoprotective and antiapoptotic activities of SHM are abolished by the inhibition of HO-1 signaling. In further study, SHM restored the skin dryness and skin barrier disruption in UVB-exposed human keratinocytes. Based to these results, our study suggests that SHM protects the cells against UVB-induced cellular damages through the Nrf2/HO-1/NF-κB p65 signaling pathway and may be potentially useful for the prevention of UVB-induced skin damage.

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The Protective Effects of Fucosterol Against Skin Damage in UVB-Irradiated Human Dermal Fibroblasts

Marine Biotechnology ◽

10.1007/s10126-013-9554-8 ◽

2013 ◽

Vol 16 (3) ◽

pp. 361-370 ◽

Cited By ~ 26

Author(s):

Eunson Hwang ◽

Sang-Yong Park ◽

Zheng-wang Sun ◽

Heon-Sub Shin ◽

Don-Gil Lee ◽

...

Keyword(s):

Dermal Fibroblasts ◽

Protective Effects ◽

Human Dermal Fibroblasts ◽

Skin Damage

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Protective effects of hawthorn (Crataegus pinnatifida ) polyphenol extract against UVB-induced skin damage by modulating the p53 mitochondrial pathway in vitro and in vivo

Journal of Food Biochemistry ◽

10.1111/jfbc.12708 ◽

2018 ◽

Vol 43 (2) ◽

pp. e12708 ◽

Cited By ~ 2

Author(s):

Suwen Liu ◽

Qianqian Sui ◽

Jian Zou ◽

Yanxue Zhao ◽

Xuedong Chang

Keyword(s):

Mitochondrial Pathway ◽

Protective Effects ◽

Skin Damage ◽

Crataegus Pinnatifida

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Protective Effects of Dietary Grape on UVB-Mediated Cutaneous Damages and Skin Tumorigenesis in SKH-1 Mice

Cancers ◽

10.3390/cancers12071751 ◽

2020 ◽

Vol 12 (7) ◽

pp. 1751 ◽

Cited By ~ 1

Author(s):

Charlotte A. Mintie ◽

Anna K. Musarra ◽

Chandra K. Singh ◽

Mary A. Ndiaye ◽

Ruth Sullivan ◽

...

Keyword(s):

Mast Cell ◽

Early Stage ◽

Cell Infiltration ◽

Protective Effects ◽

Skin Damage ◽

Short Term ◽

Skin Tumorigenesis ◽

Upstream Regulator ◽

Short Term Study ◽

Uvb Exposure

Non-melanoma skin cancers (NMSCs) are the most diagnosed cancers in the US and occur more frequently in males. We previously demonstrated chemoprotective effects of dietary grape powder (GP) against UVB-mediated skin tumorigenesis in female SKH-1 mice. To expand on this, here, we determined the effects of GP in a short-term UVB exposure protocol (0 or 5% GP, followed by UVB every other day for 2 weeks) in male and female SKH-1 mice, as well as explored any sex-related differences in UVB carcinogenesis via male SKH-1 mice (0, 3, or 5% GP; UVB twice weekly for 28 weeks). In the short-term study, we found that GP protects against early-stage epithelial hyperplasia and mast cell infiltration in both sexes. In the long term, GP markedly reduced tumor counts and malignant conversion, along with significant decreases in mast cell infiltration, serum IgE and Eotaxin. We also found inhibition of P38 phosphorylation and reduced PCNA, Ki67 and BCL2 levels, suggesting that the anti-inflammatory effects of GP inhibits P38, acting as an upstream regulator to inhibit proliferation and reduce tumor cell survival. Together, GP appears to protect against UVB-mediated skin damage and carcinogenesis in SKH-1 mice and should be explored further as a supplement for NMSC prevention.

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Topical Application of Aronia melanocarpa Extract Rich in Chlorogenic Acid and Rutin Reduces UVB-Induced Skin Damage via Attenuating Collagen Disruption in Mice

Molecules ◽

10.3390/molecules25194577 ◽

2020 ◽

Vol 25 (19) ◽

pp. 4577

Author(s):

Young Her ◽

Tae-Kyeong Lee ◽

Jong Dai Kim ◽

Bora Kim ◽

Hyejin Sim ◽

...

Keyword(s):

Chlorogenic Acid ◽

High Performance ◽

Collagen Type ◽

Collagen Type I ◽

Ultraviolet B ◽

Type I ◽

Protective Effects ◽

Dorsal Skin ◽

Skin Damage ◽

Aronia Melanocarpa

Aronia melanocarpa, a black chokeberry, contains high levels of phenolic acids and polyphenolic flavonoids and displays antioxidative and anti-inflammatory effects. Through high-performance liquid chromatography for extracts from Aronia melanocarpa, we discovered that the extract contained chlorogenic acid and rutin as major ingredients. In this study, we examined the protective effects of the extract against ultraviolet B- (UVB)-induced photodamage in the dorsal skin of institute of cancer research (ICR) mice. Their dorsal skin was exposed to UVB, thereafter; the extract was topically applied once a day for seven days. Photoprotective properties of the extract in the dorsal skin were investigated by clinical skin severity score for skin injury, hematoxylin and eosin staining for histopathology, Masson’s trichrome staining for collagens. In addition, we examined change in collagen type I and III, and matrix metalloproteinase (MMP)-1 and MMP-3 by immunohistochemistry. In the UVB-exposed mice treated with the extract, UVB-induced epidermal damage was significantly ameliorated, showing that epidermal thickness was moderated. In these mice, immunoreactivities of collagen type I and III were significantly increased, whereas immunoreactivities of MMP-1 and 3 were significantly decreased compared with those in the UVB-exposed mice. These results indicate that treatment with Aronia melanocarpa extract attenuates UV-induced photodamage by attenuating UVB-induced collagen disruption: these findings might be a result of the chlorogenic acid and rutin contained in the extract. Based on the current results, we suggest that Aronia melanocarpa can be a useful material for developing photoprotective adjuvant.

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Protective effects of chitosan and liposomal formulation for enzymatic degradation of insulin and their absorption promoting effects in intestinal mucous layer.

Drug Delivery System ◽

10.2745/dds.12.347 ◽

1997 ◽

Vol 12 (5) ◽

pp. 347-352 ◽

Cited By ~ 6

Author(s):

Hirofumi Takeuchi ◽

Yuka Shimotori ◽

Hiromitsu Yamamoto ◽

Tomoaki Hino ◽

Yoshiaki Kawashima

Keyword(s):

Enzymatic Degradation ◽

Liposomal Formulation ◽

Protective Effects ◽

Mucous Layer

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The Protective Effects of a Combination of an Arginine Silicate Complex and Magnesium Biotinate Against UV-Induced Skin Damage in Rats

Frontiers in Pharmacology ◽

10.3389/fphar.2021.657207 ◽

2021 ◽

Vol 12 ◽

Author(s):

Demet Cicek ◽

Betul Demir ◽

Cemal Orhan ◽

Mehmet Tuzcu ◽

Ibrahim Hanifi Ozercan ◽

...

Keyword(s):

Mammalian Target Of Rapamycin ◽

Inflammatory Factors ◽

High Dose ◽

Activator Protein 1 ◽

Protective Effects ◽

Uvb Radiation ◽

Skin Damage ◽

Sprague Dawley ◽

Uvb Exposure ◽

Silicate Complex

The purpose of this study was to observe the effects of a novel combination of inositol-stabilized arginine silicate complex (ASI) and magnesium biotinate (MgB) on the prevention of skin damage after UVB exposure in rats. Forty-nine Sprague-Dawley rats were randomized into one of the following groups (Farage et al., 2008): NC, normal control (Gragnani et al., 2014), SC, shaved control (Pandel et al., 2013), UVB (exposed to UVB radiation) (Binic et al., 2013), ASI + MgB-L (Low Dose) (Dunaway et al., 2018), ASI + MgB-H (High Dose) (Dorner et al., 2009), ASI + MgB-L + MgB cream (Durmus et al., 2017), ASI + MgB-H + MgB cream. The results showed that ASI + MgB treatment alleviated the macroscopic and histopathological damages in the skin of rats caused by UVB exposure. Skin elasticity evaluation showed a similar trend. ASI + MgB increased serum Mg, Fe, Zn, Cu, Si, biotin, and arginine concentrations and skin hydroxyproline and biotinidase levels while decreasing skin elastase activity (p < 0.05) and malondialdehyde (MDA) concentration (p < 0.001). Moreover, ASI + MgB treatment increased skin levels of biotin-dependent carboxylases (ACC1, ACC2, PC, PCC, MCC) and decreased mammalian target of rapamycin (mTOR) pathways and matrix metalloproteinase protein levels by the regulation of the activator protein 1 (AP-1), and mitogen activated protein kinases (MAPKs) signaling pathways. In addition, ASI + MgB caused lower levels of inflammatory factors, including TNF-α, NFκB, IL-6, IL-8, and COX-2 in the skin samples (p < 0.05). The levels of Bax and caspase-3 were increased, while anti-apoptotic protein Bcl-2 was decreased by UVB exposure, which was reversed by ASI + MgB treatment. These results show that treatment with ASI and MgB protects against skin damage by improving skin appearance, elasticity, inflammation, apoptosis, and overall health.

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Geniposide, a Principal Component of Gardeniae Fructus, Protects Skin from Diesel Exhaust Particulate Matter-Induced Oxidative Damage

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2021/8847358 ◽

2021 ◽

Vol 2021 ◽

pp. 1-12

Author(s):

Ly Thi Huong Nguyen ◽

Sang Hyun Ahn ◽

Uy Thai Nguyen ◽

In Jun Yang ◽

Heung Mook Shin

Keyword(s):

Particulate Matter ◽

Diesel Exhaust ◽

Principal Component ◽

Skin Lesions ◽

Hacat Cells ◽

Ros Production ◽

Protective Effects ◽

Skin Damage ◽

Diesel Exhaust Particulate ◽

Exhaust Particulate

Gardeniae Fructus (GF) is the fruit of Gardenia jasminoides Ellis and is traditionally prescribed to treat pyogenic infections and skin ulcers. This study investigated the protective effects of GF and the underlying mechanism responsible for these effects on diesel exhaust particulate matter- (DEP-) induced skin damage. The protective effects of an ethanolic extract of GF (GFE) and its constituents (geniposidic acid, gardenoside, geniposide, chlorogenic acid, and genipin) were examined by analyzing reactive oxygen species (ROS) production, apoptosis, and tight junction (TJ) protein expression in HaCaT cells. Treatment with GFE dose-dependently inhibited intracellular ROS production and apoptosis by regulating the protein expressions of Bax, Bcl-2, and cytochrome C in DEP-stimulated (100 μg/ml) HaCaT cells. Mechanistic studies revealed that the protective effects of GFE were related to its activation of Nrf2 and HO-1 signaling in HaCaT cells. Geniposide, a main constituent of GFE, enhanced the expression of occludin in DEP-stimulated HaCaT cells. Furthermore, topical application of geniposide reduced the expressions of 8-OHdG and Bax and increased the expression of occludin in the dorsal skin lesions of DEP-stimulated mice. Gardeniae Fructus and its main component geniposide are potential candidates for the repair of DEP-induced skin damage due to their antioxidant and antiapoptotic activities.

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Topical Application of Liposomal Antioxidant's for Protection Against CEES Induced Skin Damage

10.21236/ada449955 ◽

2006 ◽

Author(s):

William L. Stone ◽

Victor Paromov ◽

Hongsong Yang ◽

Min Qui

Keyword(s):

Topical Application ◽

Skin Damage

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