Characterization of a Laboratory-Scale Container for Freezing Protein Solutions with Detailed Evaluation of a Freezing Process Simulation

Analyses of ultrathin cryosections are generally performed after freeze-drying because the presence of water renders the specimens highly susceptible to radiation damage. The water content of a subcellular compartment is an important quantity that must be known, for example, to convert the dry weight concentrations of ions to the physiologically more relevant molar concentrations. Water content can be determined indirectly from dark-field mass measurements provided that there is no differential shrinkage between compartments and that there exists a suitable internal standard. The potential advantage of a more direct method for measuring water has led us to explore the use of electron energy loss spectroscopy (EELS) for characterizing biological specimens in their frozen hydrated state.We have obtained preliminary EELS measurements from pure amorphous ice and from cryosectioned frozen protein solutions. The specimens were cryotransfered into a VG-HB501 field-emission STEM equipped with a 666 Gatan parallel-detection spectrometer and analyzed at approximately −160 C.

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Design, Installation, and Performance Characterization of a Laboratory-Scale Solar Thermal System for Experiments in Solar Energy Utilization

Volume 5: Education and Globalization; General Topics ◽

10.1115/imece2012-86361 ◽

2012 ◽

Author(s):

Stephanie Drozek ◽

Christopher Damm ◽

Ryan Enot ◽

Andrew Hjortland ◽

Brandon Jackson ◽

...

Keyword(s):

Renewable Energy ◽

Solar Energy ◽

Laboratory Scale ◽

Energy Utilization ◽

Solar Thermal ◽

Thermal System ◽

Performance Characterization ◽

And Performance ◽

Solar Thermal System

The purpose of this paper is to describe the implementation of a laboratory-scale solar thermal system for the Renewable Energy Systems Laboratory at the Milwaukee School of Engineering (MSOE). The system development began as a student senior design project where students designed and fabricated a laboratory-scale solar thermal system to complement an existing commercial solar energy system on campus. The solar thermal system is designed specifically for educating engineers. This laboratory equipment, including a solar light simulator, allows for variation of operating parameters to investigate their impact on system performance. The equipment will be utilized in two courses: Applied Thermodynamics, and Renewable Energy Utilization. During the solar thermal laboratories performed in these courses, students conduct experiments based on the American Society of Heating, Refrigeration and Air-Conditioning Engineers (ASHRAE) 93-2010 standard for testing and performance characterization of solar thermal systems. Their measurements are then used to quantify energy output, efficiency and losses of the system and subsystem components.

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Prediction and characterization of the stability enhancing effect of the Cherry-Tag™ in highly concentrated protein solutions by complex rheological measurements and MD simulations

International Journal of Pharmaceutics ◽

10.1016/j.ijpharm.2017.08.068 ◽

2017 ◽

Vol 531 (1) ◽

pp. 360-371 ◽

Cited By ~ 6

Author(s):

Pascal Baumann ◽

Marie-Therese Schermeyer ◽

Hannah Burghardt ◽

Cathrin Dürr ◽

Jonas Gärtner ◽

...

Keyword(s):

Md Simulations ◽

Protein Solutions ◽

Enhancing Effect ◽

Rheological Measurements ◽

The Stability

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DEVELOPMENT AND APPLICATION OF A CONCENTRATION PROBE FOR MIXING FLOWS TRACKING IN TURBOMACHINERY APPLICATIONS

Journal of Turbomachinery ◽

10.1115/1.4052419 ◽

2021 ◽

pp. 1-35

Author(s):

Giulia Babazzi ◽

Tommaso Bacci ◽

Alessio Picchi ◽

Tommaso Fodelli ◽

Tommaso Lenzi ◽

...

Keyword(s):

Gas Turbines ◽

Thermal Field ◽

Oxygen Sensor ◽

Point Of View ◽

Interface Plane ◽

Tracer Gas ◽

Precise Knowledge ◽

Detailed Evaluation ◽

Detection Unit

Abstract Modern gas turbines present important temperature distortions in the core-engine flowpath, mainly in the form of hot and cold streaks. As they highly influence turbines performance and lifetime, the precise knowledge of the thermal field evolution through the combustor and the high-pressure turbine is fundamental. The majority of past studies investigated streaks migrations directly examining the thermal field, while a limited amount of experimental work employed approaches based on the detection of tracer gases. The latter approach provides a more detailed evaluation of the evolution and mixing of the different flows. However, the slow time response due to the employment of sampling probes and gas analysers make the investigation extremely time consuming. In this study a commercial oxygen sensor element and its excitation/detection unit were integrated into a newly developed probe to carry out local tracer gas concentration measurements exploiting the fluorescence behaviour. The paper summarizes the probe development and calibration activities, with the characterization of its accuracy for different flow conditions. Finally, two probe applications are described: firstly the probe was used to detect tracer gas concentrations on a jet flow; afterwards it was traversed on the interface plane between a non-reactive, lean combustor simulator and the NGV cascade. The probe has proven to provide accurate and reliable measurements both from a quantitative and qualitative point of view even in highly 3D flow fields typical of gas turbines conditions.

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Laboratory-Scale Characterization of a Purple Rice Variety: Examination of Milling Quality and Determination of Anthocyanin and Oil Concentration across the Bran Layer

Transactions of the ASABE ◽

10.13031/2013.42226 ◽

2012 ◽

Vol 55 (4) ◽

pp. 1577-1582 ◽

Cited By ~ 1

Author(s):

R. Schramm ◽

M. Plater II ◽

N. Walker ◽

S. Jang ◽

J. Xu ◽

...

Keyword(s):

Rice Variety ◽

Laboratory Scale ◽

Milling Quality ◽

Oil Concentration ◽

Scale Characterization

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Structural Characterization of a Plutonium Sequestering Agent Complex by Synchrotron X-Ray Diffraction

MRS Proceedings ◽

10.1557/proc-986-0986-oo08-01 ◽

2006 ◽

Vol 986 ◽

Author(s):

Anne E. V. Gorden ◽

G. Szigethy ◽

D. K. Shuh ◽

B. E. F. Tiedemann ◽

J. Xu ◽

...

Keyword(s):

National Laboratory ◽

Selective Extraction ◽

Biological Transport ◽

X Ray Diffraction ◽

X Ray ◽

Selective Ligand ◽

Detailed Evaluation ◽

Biomimetic Approach ◽

Solid State Structures

AbstractNew ligands and materials are required that can coordinate, sense, and purify actinides for selective extraction and reduction of toxic, radioactive wastes from the mining and purification of actinides. The similarities in the chemical, biological transport, and distribution properties of Fe(III) and Pu(IV) inspired a biomimetic approach to the development of sequestering agents for actinides. A detailed evaluation of the structure and bonding of actinide coordinating ligands like these is important for the design of new selective ligand systems. Knowing the difficulty with working with the crystals resulting from these ligand systems and safe handling considerations for working with Pu, procedures were developed that utilize the Advanced Light Source of Lawrence Berkeley National Laboratory to determine the solid-state structures of Pu complexes by X-ray diffraction.

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Comparative Evaluation of Compacting Process for Base Materials using Lab Compaction Methods

Transportation Research Record Journal of the Transportation Research Board ◽

10.1177/0361198119837953 ◽

2019 ◽

Vol 2673 (4) ◽

pp. 558-567 ◽

Cited By ~ 2

Author(s):

Xiaoyang Jia ◽

Wei Hu ◽

Pawel Polaczyk ◽

Hongren Gong ◽

Baoshan Huang

Keyword(s):

Dynamic Response ◽

Laboratory Testing ◽

Comparative Evaluation ◽

Laboratory Scale ◽

Dynamic Responses ◽

Comparative Experiment ◽

Base Materials ◽

Superpave Gyratory Compactor ◽

Precise Characterization

Precise characterization of the compactability of aggregates and soils in the laboratory has always been a challenge to pavement technologists. The present study investigated compactability of aggregates and soils through the comparison of three laboratory compaction methods, the Superpave Gyratory Compactor, the Marshall impacting hammer, and a vibratory compacting machine. Accelerometers were attached to the Marshall impacting hammer and a laboratory scale vibratory compactor to capture the dynamic response of soils and aggregates during compaction. The results from this comparative experiment indicated that there was a consistent relationship between the stiffness of soils and aggregates and the dynamic responses from impaction and vibration, which can be used to better characterize the compactability of different paving materials through laboratory testing.

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Performance Evaluation of a Robot-Mounted Interferometer for an Industrial Environment

Sensors ◽

10.3390/s20010257 ◽

2020 ◽

Vol 20 (1) ◽

pp. 257

Author(s):

Istvan Biro ◽

Peter Kinnell

Keyword(s):

Sensor Deployment ◽

Light Weight ◽

Multi Scale ◽

Interferometric Sensors ◽

Evaluation Approach ◽

Independent Evaluation ◽

Detailed Evaluation ◽

Evaluation Approaches ◽

Comprehensive Characterization

High value manufacturing requires production-integrated, fast, multi-sensor and multi-scale inspection. To meet this need, the robotic deployment of sensors within the factory environment is becoming increasingly popular. For microscale measurement applications, robot-mountable versions of high-resolution instruments, that are traditionally deployed in a laboratory environment, are now becoming available. However, standard methodologies for the evaluation of these instruments, particularly when mounted to a robot, have yet to be fully defined, and therefore, there is limited independent evaluation data to describe the potential performance of these systems. In this paper, a detailed evaluation approach is presented for light-weight robot mountable scanning interferometric sensors. Traditional evaluation approaches are considered and extended to account for robotic sensor deployment within industrial environments. The applicability and value of proposed evaluation is demonstrated through the comprehensive characterization of a Heliotis H6 interferometric sensors. The results indicate the performance of the sensor, in comparison to a traditional laboratory-based system, and demonstrate the limits of the sensor capability. Based-on the evaluation an effective strategy for robotic deployment of the sensor is demonstrated.

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