Bioactive peptides identified from enzymatic hydrolysates of sturgeon skin

Bioactive peptides are short chain of amino acids (usually 2-20) that are linked by amide bond in a specific sequence which have some biological effects in animals or humans. These can be of diverse origin like plant, animal, fish, microbe, marine organism or even synthetic. They are successfully used in the management of many diseases. In recent years increased attention has been raised for its effects and mechanism of action in various disease conditions like cancer, immunity, cardiovascular disease, hypertension, inflammation, diabetes, microbial infections etc. Bioactive peptides are more bioavailable and less allergenic when compared to total proteins. Food derived bioactive peptides have health benefits and its demand has increased tremendously over the past decade. This review gives a view on last two years research on potential bioactive peptides derived from food which have significant therapeutic effects.

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Bioactive Peptides Sensitize Cells to Anticancer Effects of Oxaliplatin in Human Colorectal Cancer Xenografts in Nude Mice

Protein and Peptide Letters ◽

10.2174/0929866526666190405124955 ◽

2019 ◽

Vol 26 (7) ◽

pp. 512-522

Author(s):

Xian Li ◽

Long Xia ◽

Xiaohui Ouyang ◽

Qimuge Suyila ◽

Liya Su ◽

...

Keyword(s):

Quality Of Life ◽

Colorectal Cancer ◽

Nude Mice ◽

Low Dose ◽

Bioactive Peptides ◽

Human Colorectal Cancer ◽

Short Term ◽

Hct 116

Background: Despite new agent development and short-term benefits in patients with Colorectal Cancer (CRC), metastatic CRC cure rates have not improved due to high rates of oxaliplatin resistance and toxicity. There is an urgent need for effective tools to prevent and treat CRC and reduce morbidity and mortality of CRC patients. Exploring the effects of bioactive peptides on the antitumor to CRC was of vital importance to the clinical application. Objective: This study aimed to investigate the therapeutic impact of Anticancer Bioactive Peptides (ACBP) on anticancer effect of oxaliplatin (LOHP) in human colorectal cancer xenografts models in nude mice. Methods: HCT-116 cells were cultured in vitro via CCK-8 assays and the absorbance was measured at 450 nm. Apoptosis and cell cycle were assessed by Flow Cytometry (FCM) in vitro. HCT-116 human colorectal cancer cells inoculated subcutaneously in nude mice of treatment with PBS (GG), ACBP, LOHP, ACBP+LOHP (A+L) in vivo. The quality of life was assessed by dietary amount of nude mice, the weight of nude mice, inhibition rates, tumor weight and tumor volume. Immunohistochemistry and RT-qPCR method was conducted to determine the levels of apoptosisregulating proteins/genes in transplanted tumors. Results: ACBP induced substantial reductions in viable cell numbers and apoptosis of HCT116 cells in combined with LOHP in vitro. Compared with the control GG group, ACBP combined low dose oxaliplatin (U) group demonstrated significantly different tumor volume, the rate of apoptosis, the expression levels of Cyt-C, caspase-3,8,9 proteins and corresponding RNAs (P<0.05). The expression of pro-apoptotic proteins in the cytoplasm around the nucleus was significantly enhanced by ACBP. Short term intermittent use of ACBP alone indicted a certain inhibitory effect on tumor growth, and improve the quality of life of tumor bearing nude mice. Conclusion: ACBP significantly increased the anti-cancer responses of low dose oxaliplatin (L-LOHP), thus, significantly improving the quality of life of tumor-bearing nude mice.

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Marine-Derived Bioactive Peptides as New Anticoagulant Agents: A Review

Current Protein and Peptide Science ◽

10.2174/13892037113149990042 ◽

2013 ◽

Vol 14 (3) ◽

pp. 199-204 ◽

Cited By ~ 17

Author(s):

Rim Nasri ◽

Moncef Nasri

Keyword(s):

Bioactive Peptides ◽

Anticoagulant Agents

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Marine Bioactive Peptides as Potential Antioxidants

Current Protein and Peptide Science ◽

10.2174/13892037113149990041 ◽

2013 ◽

Vol 14 (3) ◽

pp. 189-198 ◽

Cited By ~ 18

Author(s):

Dai-Hung Ngo ◽

Se-Kwon Kim

Keyword(s):

Bioactive Peptides

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Chromatographic Characterization and In Vitro Bioactivity Evaluation of Lactobacillus helveticus Hydrolysates upon Fermentation of Different Substrates

Applied Sciences ◽

10.3390/app11020811 ◽

2021 ◽

Vol 11 (2) ◽

pp. 811

Author(s):

Federica Ianni ◽

Alessandra Anna Altomare ◽

Beniamino T. Cenci-Goga ◽

Francesca Blasi ◽

Luca Grispoldi ◽

...

Keyword(s):

Liquid Chromatography ◽

Proteolytic Activity ◽

Bioactive Peptides ◽

Biological Activities ◽

Skim Milk ◽

Brain Heart Infusion ◽

Starter Cultures ◽

Lactobacillus Helveticus ◽

Food Sources

Among various food sources, milk proteins remain the major vector for functional peptides endowed with several biological activities. Particularly, the proteolytic activity of lactic acid bacteria during milk fermentation has been one of the most followed strategies to produce bioactive peptides. In the present study, the exploration of the activity of several starter cultures, at different fermentation times, was firstly investigated by reversed phase-high performance liquid chromatography. Among the tested strains, Lactobacillus helveticus showed a higher proteolytic activity and it was submitted to further investigations by changing the fermentation substrate (skim milk, brain heart infusion, peptone water) as well as the extraction strategy (trichloroacetic acid vs. glass beads). The chromatographic analyses and the in vitro antioxidant and antihypertensive assays highlighted considerable differences for L. helveticus hydrolysates from different substrates, while a negligible impact by the two extraction protocols emerged. Furthermore, nano-high pressure liquid chromatography coupled with a high resolution mass spectrometry analyzer allowed the preliminary discrimination of fractions from fermented skim milk, likely responsible for the found activity. The obtained results suggest the possibility of varying the fermentation parameters in order to maximize the functional effects of the bioactive peptides.

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Fish Waste: From Problem to Valuable Resource

Marine Drugs ◽

10.3390/md19020116 ◽

2021 ◽

Vol 19 (2) ◽

pp. 116

Author(s):

Daniela Coppola ◽

Chiara Lauritano ◽

Fortunato Palma Esposito ◽

Gennaro Riccio ◽

Carmen Rizzo ◽

...

Keyword(s):

Circular Economy ◽

Bioactive Peptides ◽

Added Value ◽

Environmental Concerns ◽

Economic Sector ◽

Valuable Resource ◽

Fish Waste ◽

The World ◽

Global Population ◽

Aquaculture Production

Following the growth of the global population and the subsequent rapid increase in urbanization and industrialization, the fisheries and aquaculture production has seen a massive increase driven mainly by the development of fishing technologies. Accordingly, a remarkable increase in the amount of fish waste has been produced around the world; it has been estimated that about two-thirds of the total amount of fish is discarded as waste, creating huge economic and environmental concerns. For this reason, the disposal and recycling of these wastes has become a key issue to be resolved. With the growing attention of the circular economy, the exploitation of underused or discarded marine material can represent a sustainable strategy for the realization of a circular bioeconomy, with the production of materials with high added value. In this study, we underline the enormous role that fish waste can have in the socio-economic sector. This review presents the different compounds with high commercial value obtained by fish byproducts, including collagen, enzymes, and bioactive peptides, and lists their possible applications in different fields.

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