A Facile Strategy for Fabricating Tissue Engineering Scaffolds with Sophisticated Prevascularized Networks for Bulk Tissue Regeneration

2019 ◽  
Vol 304 (5) ◽  
pp. 1800642 ◽  
Author(s):  
Sui‐Hong Liu ◽  
Hai‐Guang Zhang ◽  
Shuai Li ◽  
Cheng‐Yan Sun ◽  
Qing‐Xi Hu
Keyword(s):  
Tissue Engineering ◽  
Tissue Regeneration ◽  
Tissue Engineering Scaffolds ◽  
Bulk Tissue

scholarly journals High Nanodiamond Content-PCL Composite for Tissue Engineering Scaffolds

Nanomaterials ◽  
2020 ◽  
Vol 10 (5) ◽  
pp. 948
Author(s):  
Kate Fox ◽  
Rahul Ratwatte ◽  
Marsilea A. Booth ◽  
Hoai My Tran ◽  
Phong A. Tran
Keyword(s):  
Tissue Engineering ◽  
Surface Roughness ◽  
Tensile Strength ◽  
Composite Material ◽  
Tissue Regeneration ◽  
Composite Films ◽  
Detonation Nanodiamond ◽  
3D Scaffold ◽  
Tissue Engineering Scaffolds ◽  
Osteoblast Adhesion

Multifunctional scaffolds are becoming increasingly important in the field of tissue engineering. In this research, a composite material is developed using polycaprolactone (PCL) and detonation nanodiamond (ND) to take advantage of the unique properties of ND and the biodegradability of PCL polymer. Different ND loading concentrations are investigated, and the physicochemical properties of the composites are characterized. ND-PCL composite films show a higher surface roughness and hydrophilicity than PCL alone, with a slight decrease in tensile strength and a significant increase in degradation. Higher loading of ND also shows a higher osteoblast adhesion than the PCL alone sample. Finally, we show that the ND-PCL composites are successfully extruded to create a 3D scaffold demonstrating their potential as a composite material for tissue regeneration.

Nanocomposite Scaffolds for Bone Tissue Engineering: Design, Fabrication, Surface Modification and Sustained Release of Growth Factor

2011 ◽  
Vol 1301 ◽  
Author(s):  
Min Wang ◽  
Bin Duan
Keyword(s):  
Tissue Engineering ◽  
Growth Factor ◽  
Bone Tissue ◽  
Tissue Regeneration ◽  
Tissue Repair ◽  
Nanocomposite Material ◽  
Human Bone ◽  
Tissue Engineering Scaffolds ◽  
Form Complex ◽  
Nanocomposite Scaffolds

ABSTRACTFrom the material point of view, the extracellular matrix (ECM) of bone is a natural nanocomposite consisting of an organic matrix (mainly collagen) and inorganic nanofillers (bone apatite) which are inserted in a parallel way into the collagen fibrils. For human bone tissue repair or regeneration, nanocomposites consisting of a biodegradable polymer matrix and nano-sized fillers such as bioactive ceramics or glasses, which mimic the hierarchical structure of bone, are considered a promising strategy. Combining living cells with biodegradable materials and/or bioactive component(s), the concept of tissue engineering first elucidated in the early 1990s represented a paradigm shift from tissue grafting, with autografts being the gold standard, or even completely from prosthesis implantation. In scaffold-based tissue engineering, scaffolds play an important role for tissue regeneration. Currently, acellular scaffolds with or without biomolecules such as growth factors are considered as an effective strategy for certain tissue repair due to their relatively low costs and easier process to gain surgeons’ acceptance and regulatory approval. In the current study, integrating an advanced manufacturing technique, nanocomposite material and controlled delivery of growth factor to form multifunctional tissue engineering scaffolds was investigated. Three-dimensional, osteoconductive and totally biodegradable calcium phosphate (Ca-P)/poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) nanocomposite scaffolds with customized architecture, controlled porosity and interconnecting pores were designed and fabricated using selective laser sintering (SLS). The surface of nanocomposite scaffolds was modified with gelatin and then heparin, which facilitated the incorporation of a growth factor, recombinant human bone morphogenetic protein-2 (rhBMP-2). Experimental results demonstrated the effectiveness of this strategy in guiding the osteogenic differentiation of mesenchymal stem cells. Together with osteoconductive nanocomposite material and controlled growth factor delivery, the use of SLS technique to form complex scaffolds provides a promising route towards individualized bone tissue regeneration.

scholarly journals 3D Electrospun Nanofiber-Based Scaffolds: From Preparations and Properties to Tissue Regeneration Applications

2021 ◽  
Vol 2021 ◽  
pp. 1-22
Author(s):  
Shanshan Han ◽  
Kexin Nie ◽  
Jingchao Li ◽  
Qingqing Sun ◽  
Xiaofeng Wang ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Tissue Regeneration ◽  
Cardiac Tissue ◽  
Three Dimensional ◽  
Electrospun Nanofibers ◽  
Porous Matrix ◽  
Electrospun Nanofiber ◽  
Tissue Engineering Scaffolds ◽  
3D Scaffolds

Electrospun nanofibers have been frequently used for tissue engineering due to their morphological similarities with the extracellular matrix (ECM) and tunable chemical and physical properties for regulating cell behaviors and functions. However, most of the existing electrospun nanofibers have a closely packed two-dimensional (2D) membrane with the intrinsic shortcomings of limited cellular infiltration, restricted nutrition diffusion, and unsatisfied thickness. Three-dimensional (3D) electrospun nanofiber-based scaffolds can provide stem cells with 3D microenvironments and biomimetic fibrous structures. Thus, they have been demonstrated to be good candidates for in vivo repair of different tissues. This review summarizes the recent developments in 3D electrospun nanofiber-based scaffolds (ENF-S) for tissue engineering. Three types of 3D ENF-S fabricated using different approaches classified into electrospun nanofiber 3D scaffolds, electrospun nanofiber/hydrogel composite 3D scaffolds, and electrospun nanofiber/porous matrix composite 3D scaffolds are discussed. New functions for these 3D ENF-S and properties, such as facilitated cell infiltration, 3D fibrous architecture, enhanced mechanical properties, and tunable degradability, meeting the requirements of tissue engineering scaffolds were discovered. The applications of 3D ENF-S in cartilage, bone, tendon, ligament, skeletal muscle, nerve, and cardiac tissue regeneration are then presented with a discussion of current challenges and future directions. Finally, we give summaries and future perspectives of 3D ENF-S in tissue engineering and clinical transformation.

Decellularized bone extracellular matrix in skeletal tissue engineering

2020 ◽  
Vol 48 (3) ◽  
pp. 755-764
Author(s):  
Benjamin B. Rothrauff ◽  
Rocky S. Tuan
Keyword(s):  
Tissue Engineering ◽  
Bone Regeneration ◽  
Tissue Regeneration ◽  
Animal Studies ◽  
Tumor Resection ◽  
Regenerative Capacity ◽  
Skeletal Tissue ◽  
Large Bone

Bone possesses an intrinsic regenerative capacity, which can be compromised by aging, disease, trauma, and iatrogenesis (e.g. tumor resection, pharmacological). At present, autografts and allografts are the principal biological treatments available to replace large bone segments, but both entail several limitations that reduce wider use and consistent success. The use of decellularized extracellular matrices (ECM), often derived from xenogeneic sources, has been shown to favorably influence the immune response to injury and promote site-appropriate tissue regeneration. Decellularized bone ECM (dbECM), utilized in several forms — whole organ, particles, hydrogels — has shown promise in both in vitro and in vivo animal studies to promote osteogenic differentiation of stem/progenitor cells and enhance bone regeneration. However, dbECM has yet to be investigated in clinical studies, which are needed to determine the relative efficacy of this emerging biomaterial as compared with established treatments. This mini-review highlights the recent exploration of dbECM as a biomaterial for skeletal tissue engineering and considers modifications on its future use to more consistently promote bone regeneration.

Novel tissue engineering scaffolds as wound dressings loaded with Alkannins/Shikonins as active ingredients

2019 ◽  
Author(s):  
AS Arampatzis ◽  
K Theodoridis ◽  
E Aggelidou ◽  
KN Kontogiannopoulos ◽  
I Tsivintzelis ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Wound Dressings ◽  
Active Ingredients ◽  
Tissue Engineering Scaffolds

HYDROXYAPATITE, ALGINATE, AND CHITOSAN FOR BONE SCAFFOLDS: SPECTROSCOPY STUDY

2016 ◽  
Vol 19 (2) ◽  
pp. 93-100
Author(s):  
Lalita El Milla
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Functional Groups ◽  
Bone Growth ◽  
Three Dimensional ◽  
Dimensional Structure ◽  
Tissue Engineering Scaffolds ◽  
Hydroxyapatite Powder ◽  
Materials Used

Scaffolds is three dimensional structure that serves as a framework for bone growth. Natural materials are often used in synthesis of bone tissue engineering scaffolds with respect to compliance with the content of the human body. Among the materials used to make scafffold was hydroxyapatite, alginate and chitosan. Hydroxyapatite powder obtained by mixing phosphoric acid and calcium hydroxide, alginate powders extracted from brown algae and chitosan powder acetylated from crab. The purpose of this study was to examine the functional groups of hydroxyapatite, alginate and chitosan. The method used in this study was laboratory experimental using Fourier Transform Infrared (FTIR) spectroscopy for hydroxyapatite, alginate and chitosan powders. The results indicated the presence of functional groups PO43-, O-H and CO32- in hydroxyapatite. In alginate there were O-H, C=O, COOH and C-O-C functional groups, whereas in chitosan there were O-H, N-H, C=O, C-N, and C-O-C. It was concluded that the third material containing functional groups as found in humans that correspond to the scaffolds material in bone tissue engineering.

Regenereation in periodontics

2018 ◽  
Author(s):  
Murtaza Kaderi ◽  
Mohsin Ali ◽  
Alfiya Ali ◽  
Tasneem Kaderi
Keyword(s):  
Tissue Engineering ◽  
Clinical Practice ◽  
Periodontal Disease ◽  
Disease Progression ◽  
Tissue Regeneration ◽  
Surgical Procedures ◽  
Periodontal Regeneration ◽  
Guided Tissue Regeneration ◽  
Periodontal Tissues ◽  
Extensive Documentation

The goals of periodontal therapy are to arrest of periodontal disease progression and to attain the regeneration of the periodontal apparatus. Osseous grafting and Guided tissue regeneration (GTR) are the two techniques with the most extensive documentation of periodontal regeneration. However, these techniques offer limited potential towards regenerating the periodontal tissues. Recent surgical procedures and application of newer materials aim at greater and more predictable regeneration with the concept of tissue engineering for enhanced periodontal regeneration and functional attachment have been developed, analyzed, and employed in clinical practice

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