Magnetic Nanoparticles: Uptake and Intracellular Fate of Peptide Surface-Functionalized Silica Hybrid Magnetic Nanoparticles In Vitro (Part. Part. Syst. Charact. 2/2015)

This study aims to evaluate the efficiency of a novel in vitro technique in clot capturing and dissolving them by applying magnetic force on magnetic nanoparticles (MNP) carrying thrombolytic agents. It is a quick and simple method to protect patients from a life-threatening pulmonary embolism in an emergency to provide time for the medical team. To analyze the in vitro efficiency of nano-magnetic capturing and dissolving of clots (NCDC), different levels of process parameter including strength magnetic field (0.1, 0.2 and 0.3 T) and fluid flow rate (2.5, 5 and 7 l/min) are exposed to different blood clots sizes from 5 × 10 to 20 × 10 mm2 (length × diameter), in an in vitro flow model. The results show that by increasing the parameters to their maximum values, it is possible to immobilize 100% of the clots and dissolve around 61.4% of clots weight. In addition, the clot-dissolving is directly proportional to the magnetic field strength. NCDC is an efficient technique in immobilizing and dissolving the clots and its efficiency depends on process parameters especially the magnetic field.

Download Full-text

The Potential of Intrinsically Magnetic Mesenchymal Stem Cells for Tissue Engineering

International Journal of Molecular Sciences ◽

10.3390/ijms19103159 ◽

2018 ◽

Vol 19 (10) ◽

pp. 3159 ◽

Cited By ~ 6

Author(s):

Fransiscus Kerans ◽

Lisa Lungaro ◽

Asim Azfer ◽

Donald Salter

Keyword(s):

Tissue Engineering ◽

Stem Cells ◽

Mesenchymal Stem Cells ◽

Magnetic Nanoparticles ◽

Mammalian Cells ◽

Cell Tracking ◽

Magnetic Domain ◽

Superparamagnetic Iron Oxide ◽

In Vitro Assays

The magnetization of mesenchymal stem cells (MSC) has the potential to aid tissue engineering approaches by allowing tracking, targeting, and local retention of cells at the site of tissue damage. Commonly used methods for magnetizing cells include optimizing uptake and retention of superparamagnetic iron oxide nanoparticles (SPIONs). These appear to have minimal detrimental effects on the use of MSC function as assessed by in vitro assays. The cellular content of magnetic nanoparticles (MNPs) will, however, decrease with cell proliferation and the longer-term effects on MSC function are not entirely clear. An alternative approach to magnetizing MSCs involves genetic modification by transfection with one or more genes derived from Magnetospirillum magneticum AMB-1, a magnetotactic bacterium that synthesizes single-magnetic domain crystals which are incorporated into magnetosomes. MSCs with either or mms6 and mmsF genes are followed by bio-assimilated synthesis of intracytoplasmic magnetic nanoparticles which can be imaged by magnetic resonance (MR) and which have no deleterious effects on MSC proliferation, migration, or differentiation. The stable transfection of magnetosome-associated genes in MSCs promotes assimilation of magnetic nanoparticle synthesis into mammalian cells with the potential to allow MR-based cell tracking and, through external or internal magnetic targeting approaches, enhanced site-specific retention of cells for tissue engineering.

Download Full-text

Volumetric registration of magnetic nanoparticles for optimization of quantitative immunochromatographic assays for detection of small molecules

EPJ Web of Conferences ◽

10.1051/epjconf/201818510006 ◽

2018 ◽

Vol 185 ◽

pp. 10006 ◽

Cited By ~ 2

Author(s):

Natalia V. Guteneva ◽

Sergey L. Znoyko ◽

Alexey V. Orlov ◽

Maxim P. Nikitin ◽

Petr I. Nikitin

Keyword(s):

Magnetic Nanoparticles ◽

Small Molecules ◽

Optical Methods ◽

Magnetic Method ◽

Label Free ◽

Spectral Correlation ◽

Entire Volume ◽

Specificity And Sensitivity ◽

Particle Quantification

Precise quantitative and highly sensitive detection of small molecules (haptens) is highly demanded in medicine, food quality control, in vitro diagnostics, criminalistics, environmental monitoring, etc. In the present work, the magnetic method of particle quantification and the optical methods of spectral correlation and spectral phase interferometry complement each other for optimization of a quantitative assay for measuring concentrations of small molecules. The assay employs magnetic nanoparticles as labels in rapid immunochromatographic format. The approach was demonstrated with fluorescein as a model molecule. The interferometric label-free biosensors were employed for selection of optimal reagents that produced high specificity and sensitivity. The method of magnetic particle quantification counted the magnetic labels over the entire volume of the immunochromatographic membrane to provide their distribution along the test strip. Such distribution was used for optimization of such parameters as concentrations of the used reagents and of antibody immobilized on the labels, amount of the labels and conjugates of haptens with protein carriers to realize the advanced quantitative immunochromatographic assay.

Download Full-text

Functionalized magnetic nanoparticles as vehicles for the delivery of the antitumor drug gemcitabine to tumor cells. Physicochemical in vitro evaluation

Materials Science and Engineering C ◽

10.1016/j.msec.2012.12.009 ◽

2013 ◽

Vol 33 (3) ◽

pp. 1183-1192 ◽

Cited By ~ 22

Author(s):

J.L. Viota ◽

A. Carazo ◽

J.A. Munoz-Gamez ◽

K. Rudzka ◽

R. Gómez-Sotomayor ◽

...

Keyword(s):

Magnetic Nanoparticles ◽

Tumor Cells ◽

Antitumor Drug ◽

In Vitro Evaluation ◽

Functionalized Magnetic Nanoparticles

Download Full-text

Tetramethylguanidine-functionalized silica-coated iron oxide magnetic nanoparticles catalyzed one-pot three-component synthesis of furanone derivatives

Journal of Chemical Sciences ◽

10.1007/s12039-018-1572-7 ◽

2018 ◽

Vol 130 (12) ◽

Cited By ~ 5

Author(s):

Hamideh Ahankar ◽

Ali Ramazani ◽

Nadia Fattahi ◽

Katarzyna Ślepokura ◽

Tadeusz Lis ◽

...

Keyword(s):

Iron Oxide ◽

Magnetic Nanoparticles ◽

Functionalized Silica ◽

One Pot ◽

Iron Oxide Magnetic Nanoparticles

Download Full-text

Synthesis, characterization and in vitro validation of a magnetic zeolite nanocomposite with T2-MRI properties towards theranostic applications

Journal of Materials Chemistry B ◽

10.1039/c9tb00078j ◽

2019 ◽

Vol 7 (21) ◽

pp. 3351-3361 ◽

Cited By ~ 5

Author(s):

Natália Vilaça ◽

Juan Gallo ◽

Rui Fernandes ◽

Francisco Figueiredo ◽

António M. Fonseca ◽

...

Keyword(s):

Magnetic Nanoparticles ◽

Mri Contrast ◽

Ability To Act ◽

Magnetic Zeolite ◽

Theranostic Applications ◽

T2 Mri

This study focusses on the development of a magnetic zeolite nanocomposite as a suitable platform towards the design of a theranostic system. Herein, we explored its ability to act as a T2-MRI contrast enhancer when magnetic nanoparticles are incorporated in its structure.

Download Full-text

Remote manipulation of magnetic nanoparticles using magnetic field gradient to promote cancer cell death

10.1101/317115 ◽

2018 ◽

Author(s):

Mahendran Subramanian ◽

Arkadiusz Miaskowski ◽

Stuart Iain Jenkins ◽

Jenson Lim ◽

Jon Dobson

Keyword(s):

Magnetic Field ◽

Magnetic Nanoparticles ◽

Field Gradient ◽

Biomedical Applications ◽

Magnetic Field Gradient ◽

Field Source ◽

Remote Manipulation ◽

Cancer Cell Death ◽

The Magnetic Field

AbstractThe manipulation of magnetic nanoparticles (MNPs) using an external magnetic field, has been demonstrated to be useful in various biomedical applications. Some techniques have evolved utilizing this non-invasive external stimulus but the scientific community widely adopts few, and there is an excellent potential for more novel methods. The primary focus of this study is on understanding the manipulation of MNPs by a time-varying static magnetic field and how this can be used, at different frequencies and displacement, to manipulate cellular function. Here we explore, using numerical modeling, the physical mechanism which underlies this kind of manipulation, and we discuss potential improvements which would enhance such manipulation with its use in biomedical applications, i.e., increasing the MNP response by improving the field parameters. From our observations and other related studies, we infer that such manipulation depends mostly on the magnetic field gradient, the magnetic susceptibility and size of the MNPs, the magnet array oscillating frequency, the viscosity of the medium surrounding MNPs, and the distance between the magnetic field source and the MNPs. Additionally, we demonstrate cytotoxicity in neuroblastoma (SH-SY5Y) and hepatocellular carcinoma (HepG2) cells in vitro. This was induced by incubation with MNPs, followed by exposure to a magnetic field gradient, physically oscillating at various frequencies and displacement amplitudes. Even though this technique reliably produces MNP endocytosis and/or cytotoxicity, a better biophysical understanding is required to develop the mechanism used for this precision manipulation of MNPs, in vitro.

Download Full-text