Polymorphism of the rs1800896 IL10 promoter gene protects children from post-bronchiolitis asthma

2013 ◽  
Vol 49 (8) ◽  
pp. 800-806 ◽  
Author(s):  
Petri Koponen ◽  
Kirsi Nuolivirta ◽  
Miia Virta ◽  
Merja Helminen ◽  
Mikko Hurme ◽  
...  
Keyword(s):  
Promoter Gene ◽  
Il10 Promoter

scholarly journals Porcine Circovirus Type 2 Rep Enhances IL-10 Production in Macrophages via Activation of p38-MAPK Pathway

Viruses ◽  
2019 ◽  
Vol 11 (12) ◽  
pp. 1141
Author(s):  
Xingchen Wu ◽  
Xiaoya Wang ◽  
Tengfei Shi ◽  
Le Luo ◽  
Dan Qiao ◽  
...  
Keyword(s):  
P38 Mapk ◽  
Mapk Pathway ◽  
Porcine Circovirus Type ◽  
Porcine Circovirus Type 2 ◽  
Pcv2 Infection ◽  
Porcine Circovirus ◽  
Rep Proteins ◽  
Il10 Promoter ◽  
Later Phase

Porcine circovirus type 2 (PCV2) is one of the major threats to pig farms worldwide. Although PCV2 has been identified to promote IL-10 production, the detailed regulatory roles of PCV2 Rep for IL-10 production remain unclear. Herein, we first found that PCV2 Rep, rather than PCV1 Rep, enhanced IL-10 expression at the later phase of PCV2 infection in porcine alveolar macrophages (PAMs). Furthermore, we found that PCV2 Rep directly activated the p38-MAPK pathway to promote transcription factors NF-κB p50 and Sp1 binding to the il10 promoter, but PCV1 Rep did not. During PCV2 infection, however, PCV2 Rep promoted the binding activities of NF-κB p50 and Sp1 with the il10 promoter only at the later phase of PCV2 infection, since Rep proteins only expressed at the later phase of the infection. Moreover, silence of the thymine DNA glycosylase (TDG), a Rep-binding protein, significantly reduced the binding activities of NF-κB p50 and Sp1 with il10 promoter, resulting in the reduction of IL-10 production in PCV2-inoculated PAMs at the later phase of infection. Taken together, our results demonstrate that Rep proteins enhance IL-10 production during PCV2 infection of PAMs via activation of p38-MAPK pathways, in which host TDG is a critical mediator.

scholarly journals Genetic Diversity of CD14 Promoter Gene Polymorphism (rs2569190) is Associated With Regulation of Malaria Parasitemia and Susceptibility to Plasmodium falciparum Infection

2017 ◽  
Vol 10 ◽  
pp. 117863361772678 ◽  
Author(s):  
Olusola Ojurongbe ◽  
Roland I Funwei ◽  
Tara J Snyder ◽  
Najihah Aziz ◽  
Yi Li ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Plasmodium Falciparum ◽  
Plasmodium Falciparum Malaria ◽  
Cell Types ◽  
Infected Group ◽  
Genotypic Frequency ◽  
Experimental Cerebral Malaria ◽  
Asymptomatic Patients ◽  
Mutant Variant ◽  
Promoter Gene

CD14 is a multifunctional receptor expressed on many cell types and has been shown to mediate immune response resulting in the activation of an inflammatory cascade, with polymorphism of its promoter (rs2569190) found to be associated with susceptibility to several diseases. In malaria infection, the CD14 gene demonstrated a pathogenic profile in regulating experimental cerebral malaria, with reports of elevated levels of soluble CD14 in serum of patients but no definitive conclusion. We present a detailed analysis of genetic diversity of CD14 promoter gene (snp −159 C/T; rs2519190) polymorphism between a malaria-infected group and uninfected controls and its association with clinical parameters of disease. Genomic DNA samples obtained from 106 Plasmodium falciparum malaria–infected patients and 277 uninfected controls were elucidated with a polymerase chain reaction-restriction fragment length polymorphism (RFLP) assay. Our results show a significant diversity ( P = 3.32E−06) in the genotypic frequency (3.8% versus 22.4%) of the rs2569190 mutant variant between the malaria-infected group and controls, respectively. The mutant allele had the lowest frequency among the malaria-infected group demonstrating its necessity for infection. Mean parasitemia (parasites/μL of blood) was significantly regulated based on CD14 polymorphic profile (19 855 versus 37 041 versus 49 396 for homozygote mutants, heterozygotes, and homozygote wild type, respectively). Interestingly, we found no association between CD14 genetic variants with fever, age of patients, or anemia. How this affects disease severity between subregional and continental groups deserves further clarification, including extending these studies in a larger group and among severe and asymptomatic patients with malaria.

IL10 promoter rs6667202 polymorphism is functional in health but not in grade c periodontitis patients: A pilot study

2021 ◽  
Author(s):  
Camila S. Stolf ◽  
Catharina M. Sacramento ◽  
Hélvis E. S. Paz ◽  
Renato A. Machado ◽  
Lucas P. Ramos ◽  
...  
Keyword(s):  
Pilot Study ◽  
Il10 Promoter

scholarly journals TIMP-3 promoter gene polymorphisms in BFL

Thorax ◽  
2005 ◽  
Vol 60 (11) ◽  
pp. 974-974 ◽  
Author(s):  
R Janssen
Keyword(s):  
Gene Polymorphisms ◽  
Promoter Gene

scholarly journals A Thrombomodulin Promoter Gene Polymorphism, rs2239562, Influences Both Susceptibility to and Outcome of Sepsis

2022 ◽  
Vol 8 ◽  
Author(s):  
Eizo Watanabe ◽  
Osamu Takasu ◽  
Youichi Teratake ◽  
Teruo Sakamoto ◽  
Toshiaki Ikeda ◽  
...  
Keyword(s):  
Severe Sepsis ◽  
Protein C ◽  
Validation Cohort ◽  
University Hospital ◽  
Coagulation Cascade ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Functional Polymorphisms ◽  
Promoter Gene ◽  
Control Study

Objective: Disseminated intravascular coagulation plays a key role in the pathophysiology of sepsis. Thrombomodulin is essential in the protein C system of coagulation cascade, and functional polymorphisms influence the human thrombomodulin gene (THBD). Therefore, we conducted a multicenter study to evaluate the influence of such polymorphisms on the pathophysiology of sepsis.Methods: A collaborative case-control study in the intensive care unit (ICU) of each of five tertiary emergency centers. The study included 259 patients (of whom 125 displayed severe sepsis), who were admitted to the ICU of Chiba University Hospital, Chiba, Japan between October 2001 and September 2008 (discovery cohort) and 793 patients (of whom 271 patients displayed severe sepsis), who were admitted to the five ICUs between October 2008 and September 2012 (multicenter validation cohort). To assess the susceptibility to severe sepsis, we further selected 222 critically ill patients from the validation cohort matched for age, gender, morbidity, and severity with the patients with severe sepsis, but without any evidence of sepsis.Results: We examined whether the eight THBD single nucleotide polymorphisms (SNPs) were associated with susceptibility to and/or mortality of sepsis. Higher mortality on severe sepsis in the discovery and combined cohorts was significantly associated with the CC genotype in a THBD promoter SNP (−1920*C/G; rs2239562) [odds ratio [OR] 2.709 (1.067–6.877), P = 0.033 and OR 1.768 (1.060–2.949), P = 0.028]. Furthermore, rs2239562 SNP was associated with susceptibility to severe sepsis [OR 1.593 (1.086–2.338), P = 0.017].Conclusions: The data demonstrate that rs2239562, the THBD promoter SNP influences both the outcome and susceptibility to severe sepsis.

scholarly journals Association between Osteopontin Promoter Gene Polymorphisms and Haplotypes with Risk of Diabetic Nephropathy

2015 ◽  
Vol 4 (6) ◽  
pp. 1281-1292 ◽  
Author(s):  
Balneek Cheema ◽  
Sreenivasa Iyengar ◽  
Rajni Sharma ◽  
Harbir Kohli ◽  
Anil Bhansali ◽  
...  
Keyword(s):  
Diabetic Nephropathy ◽  
Gene Polymorphisms ◽  
Promoter Gene
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