Aromatic compounds that attract Meloidogyne species second‐stage juveniles in soil

2021 ◽  
Author(s):  
Yuji Oka

Halides that are active Friedel Craft catalysts with aromatic compounds have been used to prepare synthetic metals. Aluminium bromide AlBr 3 intercalates readily into well oriented graphite between 70 and 80 °C. Second stage intercalation compounds are formed with approximate composition (C 33 AlBr 3.6 ). Aluminium chloride AlCl 3 does not intercalate spontaneously to any appreciable extent, unless free chlorine is also present. In this case, intercalation begins at about 60 °C and reaches a limit after several hours between 70 and 80 °C to produce a first stage intercalate with approximate composition (C 18 + AlCl 4 - .AlCl 3 ). In the direction of the a -axes both halide intercalates show good metallic conductivity with p characteristics. Unlike the parent graphite, temperature coefficients of resistivity of these synthetic metals are comparable with those of natural metals, indicating that thermal vibrations of charged atoms or molecules in the crystal compound contribute a dominant scattering mechanism controlling the electrical conductivity.



Nematology ◽  
2004 ◽  
Vol 6 (5) ◽  
pp. 765-771 ◽  
Author(s):  
Philippe Jourand ◽  
Thierry Mateille ◽  
Mireille Fargette ◽  
Sylvie Rapior

AbstractThe in vitro paralysis of second-stage juveniles of Meloidogyne incognita, M. javanica and M. mayaguensis by aqueous extracts of 15 West African Crotalaria species was analysed. A multivariate analysis distinguished four groups, based on their nematostatic activity: i) C. glaucoides extracts were not active; ii) the root extracts from C. goreensis, C. lathyroides and C. perrottetii were more active than the shoot extracts; iii) the shoot extracts from C. comosa and C. cylindrocarpa were more active than the root extracts; iv) both shoot and root extracts of C. atrorubens, C. barkae, C. grantiana, C. hyssopifolia, C. pallida, C. podocarpa, C. retusa, C. senegalensis and C. sphaerocarpa have nematostatic effects depending on either the Crotalaria species or plant tissue from which the extracts were derived. However, considering the greater biomass contribution of the leaves and stems compared to the roots when the plants are used as green manure, C. barkae, C. grantiana, C. pallida and C. podocarpa are the most efficient Crotalaria species, whatever the Meloidogyne species targeted.



Plants ◽  
2020 ◽  
Vol 9 (7) ◽  
pp. 856
Author(s):  
Iro Pardavella ◽  
Eleni Nasiou ◽  
Dimitra Daferera ◽  
Panayiotis Trigas ◽  
Ioannis Giannakou

Essential oil (EO) and hydrosol (HL) isolated from an indigenous plant species Satureja hellenica were evaluated against Meloidogyne incognita and M. javanica. Particularly, the activity of extracts on a second stage juvenile’s (J2s) motility, the hatching of J2s from eggs, egg differentiation and the effect on J2s in soil were tested. A paralysis of 100% of the J2s of both species was recorded after 96 h of immersion in the essential oil, at a dose of 2000 μL/L. At the same dose, the percentage of paralyzed J2s after 48 h of immersion was more than 80%, for both Meloidogyne species. The use of hydrosol has shown encouraging results only in the dilution of 50%, where for both Meloidogyne species tested, the percentage of paralyzed J2s was more than 70% after 48 h of immersion, while the percentage was increased to 90% after 96 h of immersion. Egg differentiation was ceased after immersion, either in EO or HL. However, this decrease in egg differentiation was evident only at higher concentrations of EO and at the highest HL dilution (0.5 v/v). The hatching of M. incognita J2s was decreasing as the dose was increasing. The lowest numbers of hatched J2s were recorded at the doses of 2000 and 4000 μL/L. A clear reduction in M. javanica J2s hatching was observed as the dose was increased to 250 μL/L, a fact constantly observed as the dose was increasing up to 4000 μL/L. Lower numbers of nematodes were recorded in roots grown in infested soil after the application of EO or HL at the highest doses. The EO of S. hellenica is characterized by the presence of p-cymene (27.46%) and carvacrol (23.25%), and in a lesser extent of other constituents, such as borneol (6.79%), carvacrol methylether (6.77%), γ-terpinene (4.63%) and 4-terpineol (3.65%). Carvacrol was the major constituent found in the HL (50.12%), followed by borneol and 4-terpineol (20.42 and 6.72%, respectively).



Nematology ◽  
2001 ◽  
Vol 3 (4) ◽  
pp. 303-312 ◽  
Author(s):  
Hedwig Hirschmann ◽  
Jonathan Eisenback

AbstractFemales, males and second-stage juveniles of Meloidogyne spartinae were examined by light and scanning electron microscopy. The morphology of M. spartinae is very typical of the genus for most characters in general. However, as expected, some minor differences occur, particularly in the shape of the head, stylet and tail, and morphometrics of several characters. It is not clear at present how the karyotype of seven chromosomes in M. spartinae is related to the 13-18 chromosomes of other apparently related Meloidogyne species. The M. spartinae karyotype may represent an ancestral form from which the other species have evolved, possibly as polyploid-aneuploids. More studies are needed to clarify this point. However, M. spartinae is remarkably similar in morphology to other species and is justly placed within the genus Meloidogyne.



2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Zhen Liu ◽  
Na Qi ◽  
Yaning Luan ◽  
Xiangyang Sun

The pyrolysis of willow samples from various plant positions was analysed using thermogravimetry-Fourier transform infrared spectroscopy (TG-FTIR). The results indicate that pyrolysis can be divided into four stages. The first stage from 30 to 120°C involves free evaporation of moisture, with a mass loss of 5%. The second stage from 120 to 200°C involves the pyrolysis of hemicellulose and unstable cellulose, with a mass loss of 4%. The third stage is from 200 to 400°C, with a weight loss of 60%, in which the chemical components of wood thermally decompose and emit heat, carbon dioxide, and so on. In the final stage, which occurs above 400°C, the pyrolysis of lignin and charring of cellulose occur, with a mass loss of 10%. Moreover, in FTIR, the samples exhibit the highest absorbance during the main pyrolysis phase, from which wood vinegar ingredients mainly arise, including CO2, H2O, CO, and small amounts of hydrocarbons, alcohols, phenols, acids, esters, and aromatic compounds. Additionally, leaves are decomposed more thoroughly before the main pyrolysis phase, whereas decomposition of branches occurs fullest during this phase. Finally, we put forward some suggestions to support further research on conversion of willow into wood vinegar products.





Plants ◽  
2020 ◽  
Vol 10 (1) ◽  
pp. 46
Author(s):  
Iro Pardavella ◽  
Demetra Daferera ◽  
Theodoros Tselios ◽  
Panagiota Skiada ◽  
Ioannis Giannakou

The essential oil (EO) and hydrosol (HL) isolated from Cuminum cyminum (cumin) seeds were evaluated against the root-knot nematodes Meloidogyne incognita and M. javanica. The efficacy of extracts on the motility, hatching, and survival in soil of second-stage juveniles (J2s), and the activity on egg differentiation were tested. All J2s were paralyzed after immersion in the EO at 62.5 μL/L concentration for 96 h. Encouraging results were recorded using HL equal to or higher than 10% concentration for both Meloidogyne species tested. More than 70% paralyzed J2s were recorded after immersion for 48 h, while the percentage was increased to higher than 90% after 96 h of immersion. A clear effect on egg differentiation was observed after immersion in EO or HL. A significant decrease in egg differentiation was revealed at even low concentrations of EO while an evident decrease in egg differentiation was recorded after immersion of eggs in 50% HL dilution. Decreased hatching of M. incognita and M. javanica J2s was observed with the increase in concentration. The lowest numbers of hatched J2s were recorded when EO was used at 1000 and 2000 μL/L concentrations. A constant reduction in root-knot nematode J2 hatching was observed upon increasing the concentration of HL from 5% to 50%. The EO of C. cyminum is characterized by the presence of γ-terpinene-7-al (34.95%), cumin aldehydes (26.48), and α-terpinene-7-al (12.77%). The above constituents were observed in HL following the same order as that observed in EO. The components γ-terpinene (11.09%) and ο-cymene (6.56%) were also recorded in EO while they were absent in HL.



Author(s):  
Dale E. Bockman ◽  
L. Y. Frank Wu ◽  
Alexander R. Lawton ◽  
Max D. Cooper

B-lymphocytes normally synthesize small amounts of immunoglobulin, some of which is incorporated into the cell membrane where it serves as receptor of antigen. These cells, on contact with specific antigen, proliferate and differentiate to plasma cells which synthesize and secrete large quantities of immunoglobulin. The two stages of differentiation of this cell line (generation of B-lymphocytes and antigen-driven maturation to plasma cells) are clearly separable during ontogeny and in some immune deficiency diseases. The present report describes morphologic aberrations of B-lymphocytes in two diseases in which second stage differentiation is defective.



Author(s):  
O. L. Shaffer ◽  
M.S. El-Aasser ◽  
C. L. Zhao ◽  
M. A. Winnik ◽  
R. R. Shivers

Transmission electron microscopy is an important approach to the characterization of the morphology of multiphase latices. Various sample preparation techniques have been applied to multiphase latices such as OsO4, RuO4 and CsOH stains to distinguish the polymer phases or domains. Radiation damage by an electron beam of latices imbedded in ice has also been used as a technique to study particle morphology. Further studies have been developed in the use of freeze-fracture and the effect of differential radiation damage at liquid nitrogen temperatures of the latex particles embedded in ice and not embedded.Two different series of two-stage latices were prepared with (1) a poly(methyl methacrylate) (PMMA) seed and poly(styrene) (PS) second stage; (2) a PS seed and PMMA second stage. Both series have varying amounts of second-stage monomer which was added to the seed latex semicontinuously. A drop of diluted latex was placed on a 200-mesh Formvar-carbon coated copper grid.



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