Autoregressive Wiener filtering in a scanning electron microscopy imaging system

Improving soft tissue attachment to implant abutments is a crucial factor for enduring health and maintenance of soft peri-implant tissue health. In this in vitro study we aimed to compare the biocompatibility of three different abutment surfaces: titanium, zirconia and modified polyetheretherketone (PEEK). Surface topography, roughness and wettability were investigated with scanning electron microscopy, profilometer and contact angle meter, respectively. Human gingival epithelial keratinocytes were examined for viability, morphology, proliferation and migration by using tetrazolium salt colorimetric assay, scanning electron microscopy imaging, immunofluorescence bromodeoxyuridine analysis and scratch wound healing assays. Roughness measurements revealed differences between the investigated surfaces. Keratinocytes cultured on all examined surfaces indicated adhesion and attachment by means of scanning electron microscopy imaging. Cell viability assays showed no significant differences between the groups (p > 0.05). The modified PEEK surface similarly improved surface roughness in comparison to titanium and zirconia, which resulted in greater and equivalent cell proliferation and migration. The study methodology showed here may emphasize the importance of cell interactions with different abutment materials, which in part increases the changes of implant success. PEEK, titanium and zirconia surface types used in this study showed mostly similar epithelial biological responses.

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Strip the Stripes: Artifact Detection and Removal for Scanning Electron Microscopy Imaging

ICASSP 2019 - 2019 IEEE International Conference on Acoustics, Speech and Signal Processing (ICASSP) ◽

10.1109/icassp.2019.8683119 ◽

2019 ◽

Cited By ~ 1

Author(s):

Amirhossein Khalilian-Gourtani ◽

Mariano Tepper ◽

Victor Minden ◽

Dmitri B. Chklovskii

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Microscopy Imaging ◽

Artifact Detection ◽

Scanning Electron

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Accurate displacement field measurement with scanning electron microscopy imaging

The Journal of Strain Analysis for Engineering Design ◽

10.1177/0309324711405751 ◽

2011 ◽

Vol 46 (5) ◽

pp. 337-346 ◽

Cited By ~ 2

Author(s):

T Arikawa ◽

G Murasawa ◽

S Yoneyama

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Displacement Field ◽

Field Measurement ◽

Microscopy Imaging ◽

Scanning Electron

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Cajuputs candy impairs Candida albicans and Streptococcus mutans mixed biofilm formation in vitro

F1000Research ◽

10.12688/f1000research.20700.2 ◽

2020 ◽

Vol 8 ◽

pp. 1923

Author(s):

Siska Septiana ◽

Boy Muchlis Bachtiar ◽

Nancy Dewi Yuliana ◽

Christofora Hanny Wijaya

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Candida Albicans ◽

Streptococcus Mutans ◽

Biofilm Formation ◽

Microscopy Imaging ◽

Biofilm Inhibition ◽

Yeast Form ◽

Melaleuca Cajuputi ◽

Scanning Electron

Background: Cajuputs candy (CC), an Indonesian functional food, utilizes the bioactivity of Melaleuca cajuputi essential oil (MCEO) to maintain oral cavity health. Synergistic interaction between Candida albicans and Streptococcus mutans is a crucial step in the pathogenesis of early childhood caries. Our recent study revealed several alternative MCEOs as the main flavors in CC. The capacity of CC to interfere with the fungus-bacterium relationship remains unknown. This study aimed to evaluate CC efficacy to impair biofilm formation by these dual cariogenic microbes. Methods: The inhibition capacity of CC against mixed-biofilm comprising C. albicans and S. mutans was assessed by quantitative (crystal violet assay, tetrazolium salt [MTT] assay, colony forming unit/mL counting, biofilm-related gene expression) and qualitative analysis (light microscopy and scanning electron microscopy). Result: Both biofilm-biomass and viable cells were significantly reduced in the presence of CC. Scanning electron microscopy imaging confirmed this inhibition capacity, demonstrating morphology alteration of C. albicans, along with reduced microcolonies of S. mutans in the biofilm mass. This finding was related to the transcription level of selected biofilm-associated genes, expressed either by C. albicans or S. mutans. Based on qPCR results, CC could interfere with the transition of C. albicans yeast form to the hyphal form, while it suppressed insoluble glucan production by S. mutans. G2 derived from Mojokerto MCEO showed the greatest inhibition activity on the relationship between these cross-kingdom oral microorganisms (p < 0.05). Conclusion: In general, all CC formulas showed biofilm inhibition capacity. Candy derived from Mojokerto MCEO showed the greatest capacity to maintain the yeast form of C. albicans and to inhibit extracellular polysaccharide production by S. mutans. Therefore, the development of dual-species biofilms can be impaired effectively by the CC tested.

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Ecomorphological Study of Scales in Some Genera of the Viperid and Colubrid Snakes (Ophidia: Viperidae: Colubridae)

10.22541/au.163253921.13688743/v1 ◽

2021 ◽

Author(s):

morteza Akbarpour ◽

Nasrullah Rastegar Pouyani ◽

Behzad Fathinia ◽

Eskandar Rastegar-pouyani

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Visible Light ◽

Phylogenetic Relationships ◽

Imaging System ◽

Ecological Condition ◽

Light Reflection ◽

Body Parts ◽

Snake Species ◽

Scanning Electron

Ecomorphological studies are aimed to find out the relation between the morphology of organisms and their ecology. Many studies on reptile scale microornamentation indicate that it has important functional values. In this study microornamentation and light reflection of scales’ surface among six viperid and two colubrid snake species in relation to their habitat were examined. To compare microornamentation and light reflection analyses, skin specimens were prepared and analyzed using scanning electron microscopy and FluoVision Imaging System and spectrometer, respectively. The results showed that snake species inhabiting similar habitats had different microornamentation and vice versa. Likely the scale microstructures are more influenced by phylogenetic relationships than by the environment. In examining the scales’ reflection, different species and different body parts reflect various wavelengths of visible light, which relates to ecological condition of them.

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Effect of Nano-Silica on the Properties of Concrete and Its Interaction with Slag

Transportation Research Record Journal of the Transportation Research Board ◽

10.1177/0361198120943196 ◽

2020 ◽

pp. 036119812094319

Author(s):

Aly M. Said ◽

Mohammad Sajjadul Islam ◽

Mohamed S. Zeidan ◽

Mohamed Mahgoub

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Control Group ◽

Portland Cement Concrete ◽

Nano Silica ◽

Microscopy Imaging ◽

Mercury Intrusion ◽

Concrete Mixtures ◽

Imaging Results ◽

Scanning Electron

The current study investigates the performance of concrete incorporating ground granulated blast-furnace (GGBF) slag in the presence of colloidal nano-silica. A control group of concrete mixtures is compared with a group of mixtures with 50% slag replacement, with each group examined at two different ratios of colloidal nano-silica (3% and 6% of the total cementitious material). Subsequently, the relative performance of the two groups is compared with ordinary Portland cement concrete in relation to strength and durability properties. Evaluation included experimental examination of compressive and tensile strength, rapid chloride penetration, and porosimetry using mercury intrusion tests. Furthermore, the microstructure of the cementitious matrix was evaluated using scanning electron microscopy imaging. Results of the tested concrete mixtures indicated that nano-silica particles can improve the properties of concrete containing GGBF slag. Improvement in high early strength as well as reduction in permeability are observed. Furthermore, nano-silica caused a refinement of the pore structure and an improvement to the interfacial transition zone (ITZ) as seen through mercury intrusion porosimetry (MIP) results and scanning electron microscopy imaging, respectively.

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Efficacy of Surfactant Combined with Peracetic Acid in Removing Listeria innocua from Fresh Apples

Journal of Food Protection ◽

10.4315/0362-028x.jfp-19-064 ◽

2019 ◽

Vol 82 (11) ◽

pp. 1965-1972 ◽

Cited By ~ 5

Author(s):

EWA PIETRYSIAK ◽

JULIANNE M. KUMMER ◽

INES HANRAHAN ◽

GIRISH M. GANJYAL

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Foodborne Pathogens ◽

Peracetic Acid ◽

Sodium Dodecyl ◽

Chemical Properties ◽

Tween 20 ◽

Microscopy Imaging ◽

Cleaning Methods ◽

Scanning Electron

ABSTRACT Large amounts of water used in the apple packing process, the presence of organic matter, and difficult-to-clean equipment are vectors for contaminating apples with foodborne pathogens, such as Listeria monocytogenes. There is a need to develop new cleaning methods for fresh apples and evaluate their antimicrobial efficacy. A series of surfactants of different chemical properties (cationic lauric arginate [LAE], anionic sodium dodecyl sulfate [SDS], and nonionic Tween 20 [T20]) alone and combined with peracetic acid (PAA) were evaluated for their efficiency in the removal of L. innocua, a surrogate of L. monocytogenes, from fresh apples. Whole fresh apples were inoculated with L. innocua (7 log CFU/mL) by a dipping method, dried, and subjected to wash treatments with selected cleaning solutions (water, PAA, PAA-LAE, PAA-SDS, and PAA-T20). The contact angle between the cleaning solutions and the surface of the apples was measured. The antimicrobial activity of the cleaning solutions and the efficacy of the cleaning treatment were evaluated by enumeration of L. innocua from treated apples and visualized by scanning electron microscopy. Stem bowl and calyx cavities of the apple harbored higher bacteria concentrations (∼4.82 log CFU per apple), compared with the equatorial section (∼2.66 log CFU per apple). Addition of 0.1% of selected surfactants led to a significant decrease in surface tension of cleaning solutions and allowed better spreading on the apple surface. Surfactants combined with PAA solution resulted in higher L. innocua reduction compared with PAA alone; however, their efficacy was limited. The most effective cleaning solution was PAA-T20, with reduction of approximately 2.2 log. Scanning electron microscopy imaging confirmed that most bacteria were harbored inside the surface irregularities. PAA alone and with surfactants caused damage and deformation of bacteria cells. Cleaning apples with PAA combined with surfactants may improve microbial safety in whole apples; however, the efficiency of the decontamination treatment can be reduced because of variations in apple morphology.

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