Background: In the unpredictable times of the ongoing global coronavirus disease (COVID-19), parents worldwide are affected by stressors and strains that follow in the wake of the government-initiated distancing protocols.Objective: In a two‐wave longitudinal survey, we examined levels of parental perceived stress and symptoms of anxiety and depression among a sample of parents at two time points; in the midst of the strictest government-initiated physical distancing protocols following the onset of the COVID-19 pandemic (T1, N = 2868) and three months after the protocols discontinued (T2, n = 1489). Further, we investigated the levels of parental stress, anxiety, and depression relative to perceived relationship quality and anger aimed at child(ren) at the two time points, including subgroups based on age, sex, cultural background, civil status, education level, number of children in household, employment status, and pre-existing psychiatric diagnosis.Methods and findings:Parents were asked to fill out a set of validated questionnaires on the two measurement occasions. As expected, the findings indicate that the high levels of parental stress significantly decreased from T1 to T2, indicating that the cumulative stressors that parent’s experiences during distancing protocols declined as a function of the phaseout of the protocols. The decrease of perceived parental stress at the two time points, was accompanied by a significant decrease in symptoms of both depression and anxiety among the participating parents. Symptoms meeting the clinical cut-off for depression (23.0%) and generalized anxiety disorder (23.3%) were reported among participating parents at T1, compared to 16.8% and 13.8% respectively at T2. Reduction in depression and angry at child(ren) from T1 to T2 were further associated with a reduction in perceived parental stress. In addition, relationship quality and angry at child(ren) at T1 predicted change in parental stress. Conclusions: The findings underline some of the negative psychological impact of physical distancing protocols on parent’s health and well-being. Parents who are facing physical distancing and remaining at home with their children may be particularly vulnerable to parental stress, anxiety and depression. Uncovering the nature of how these constructs are associated to parents and families facing social crisis, such as the ongoing pandemic, can contribute to design relevant interventions to reduce parental stress and strengthen parental coping and resilience.Keywords: COVID-19 lockdown, parental stress, anxiety, depression, social distancing.