Cadmium transcriptionally regulates Scd1 expression in silver pomfret

Celastrol significantly alleviated DSS-induced colitis in mice. Lipidomics highlights alteration of several lipid markers associated with colitis. Celastrol treatment recovered SCD1 expression and restored the levels of lipid markers.

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Four weeks supplementation with Lactobacillus paracasei subsp. paracasei L. casei W8® shows modest effect on triacylglycerol in young healthy adults

Beneficial Microbes ◽

10.3920/bm2014.0058 ◽

2015 ◽

Vol 6 (1) ◽

pp. 29-39 ◽

Cited By ~ 6

Author(s):

A.T. Bjerg ◽

M. Kristensen ◽

C. Ritz ◽

K.D. Stark ◽

J.J. Holst ◽

...

Keyword(s):

Fatty Acids ◽

Energy Intake ◽

Animal Studies ◽

Blood Lipids ◽

Low Density Lipoprotein ◽

Insulin Response ◽

Density Lipoprotein ◽

Lactobacillus Paracasei ◽

Ad Libitum ◽

Scd1 Expression

The microbiota has been shown to have the potential to affect appetite and blood lipids positively in animal studies. We investigated if four weeks supplementation with Lactobacillus paracasei subsp. paracasei L. casei W8® (L. casei W8) had an effect on subjective appetite sensation, ad libitum energy intake, glucagon-like peptide 1 (GLP-1), glucose and insulin response in humans. Secondarily, we explored potential effects on blood lipids, fatty acids and stearoyl-CoA desaturase-1 (SCD1) activity in humans as well as SCD1 expression in piglets given L. casei W8 for two weeks. 64 healthy participants completed the double-blinded, randomised, controlled, parallel four weeks study with supplementation of L. casei W8 (1010 cfu) or placebo capsules. A meal test was conducted before and after the intervention, where subjective appetite, ad libitum energy intake, GLP-1, glucose and insulin response were measured. Additionally fasting blood lipids and fatty acids concentrations were measured. Sixteen piglets were randomised into two groups: L. casei W8 (1010 cfu/day) as top dressing on morning fed or no treatment. After two weeks piglets were sacrificed and tissue from ileum, jejunum and skeletal muscle were sampled for mRNA analyses of SCD1 expression. Compared to placebo, L. casei W8 did not affect appetite, ad libitum energy intake, GLP-1, glucose and insulin response and total, high-density or low-density lipoprotein cholesterol levels after four weeks intervention. Triacylglycerol decreased in the L. casei W8 group compared to placebo at week 4 (P=0.03). The C16:1n-7/C16:0 ratio, reflecting SCD1 activity, tended to decrease when having L. casei W8 (P=0.06) compared to placebo. Muscle SCD1 expression decreased in piglets supplemented with L. casei W8 compared to control. In conclusion, supplementation with L. casei W8 did not affect appetite parameters, glucose or insulin responses; but appear to be able to lower triacylglycerol levels, possibly by reducing its production.

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Morphometric Characteristics between Silver Pomfret, Pampus punctatissimus and Korean Pomfret, Pampus argenteus

Journal of Fisheries and Marine Sciences Education ◽

10.13000/jfmse.2021.8.33.4.994 ◽

2021 ◽

Vol 33 (4) ◽

pp. 994-1002

Author(s):

Hee-Woong KANG ◽

Jong-Cheol HAN ◽

Young-Guk JIN ◽

Kyung-Mi LEE ◽

Yeon-Min JEONG

Keyword(s):

Morphometric Characteristics ◽

Pampus Argenteus ◽

Silver Pomfret

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TAMI-37. STEAROYL-COA DESATURASE 1 IS ESSENTIAL FOR THE GROWTH OF IDH MUTANT GLIOMA

Neuro-Oncology ◽

10.1093/neuonc/noab196.821 ◽

2021 ◽

Vol 23 (Supplement_6) ◽

pp. vi206-vi206

Author(s):

Tomohiro Yamasaki ◽

Lumin Zhang ◽

Tyrone Dowdy ◽

Adrian Lita ◽

Mark Gilbert ◽

...

Keyword(s):

Glioma Cell ◽

De Novo ◽

Glioma Cells ◽

Model Systems ◽

De Novo Lipogenesis ◽

Wild Type ◽

Knock Out ◽

Stearoyl Coa Desaturase ◽

Scd1 Expression

Abstract BACKGROUND Increased de novo lipogenesis is a hallmark of cancer metabolism. In this study, we interrogated the role of de novo lipogenesis in IDH1 mutated glioma’s growth and identified the key enzyme, Stearoyl-CoA desaturase 1 (SCD1) that provides this growth advantage. MATERIALS ANDMETHODS We prepared genetically engineered glioma cell lines (U251 wild-type: U251WT and U251 IDHR132H mutant: U251RH) and normal human astrocytes (empty vector induced-NHA: NHAEV and IDHR132H mutant: NHARH). Lipid metabolic analysis was conducted by using LC-MS and Raman imaging microscopy. SCD1 expression was investigated by The Cancer Genome Atlas (TCGA) data analysis and Western-blotting method. Knock-out of SCD1 was conducted by using CRISPR/Cas9 and shRNA. RESULTS Previously, we showed that IDH1 mut glioma cells have increased monounsaturated fatty acids (MUFAs). TCGA data revealed IDH mut glioma shows significantly higher SCD1 mRNA expression than wild-type glioma. Our model systems of IDH1 mut (U251RH, NHARH) showed increased expression of this enzyme compared with their wild-type counterpart. Moreover, addition of D-2HG to U251WT increased SCD1 expression. Herein, we showed that inhibition of SCD1 with CAY10566 decreased relative cell number and sphere forming capacity in a dose-dependent manner. Furthermore, addition of MUFAs were able to rescue the SCD1 inhibitor induced-cell death and sphere forming capacity. Knock out of SCD1 revealed decreased cell proliferation and sphere forming ability. Decreasing lipid content from the media did not alter the growth of these cells, suggesting that glioma cells rely on de novo lipid synthesis rather than scavenging them from the microenvironment. CONCLUSION Overexpression of IDH mutant gene altered lipid composition in U251 cells to enrich MUFA levels and we confirmed that D-2HG caused SCD1 upregulation in U251WT. We demonstrated the glioma cell growth requires SCD1 expression and the results of the present study may provide novel insights into the role of SCD1 in IDH mut gliomas growth.

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Larval developmental stages of laboratory-reared silver pomfret,pampus argenteus

Ichthyological Research ◽

10.1007/bf02684233 ◽

2000 ◽

Vol 47 (2) ◽

pp. 137-141 ◽

Cited By ~ 13

Author(s):

Sulaiman Almatar ◽

Khalid Al-Abdul Elah ◽

Tawfiq Abu-Rezq

Keyword(s):

Developmental Stages ◽

Pampus Argenteus ◽

Silver Pomfret

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Sensory and chemical assessment of silver pomfret ( Pampus argenteus ) treated with Ginkgo biloba leaf extract treatment during storage in ice

Aquaculture and Fisheries ◽

10.1016/j.aaf.2017.09.003 ◽

2018 ◽

Vol 3 (1) ◽

pp. 30-37 ◽

Cited By ~ 16

Author(s):

Weiqing Lan ◽

Xu Che ◽

Qiaoling Xu ◽

Ting Wang ◽

Ruoyuan Du ◽

...

Keyword(s):

Ginkgo Biloba ◽

Leaf Extract ◽

Pampus Argenteus ◽

Chemical Assessment ◽

Ginkgo Biloba Leaf Extract ◽

Silver Pomfret ◽

Ginkgo Biloba Leaf

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Monounsaturated fatty acid antagonizes saturated fatty acid-induced cardiomyopathy via improving membrane fatty acid composition and endoplasmic reticulum stress

European Heart Journal ◽

10.1093/eurheartj/ehab724.3325 ◽

2021 ◽

Vol 42 (Supplement_1) ◽

Author(s):

T Y Yamamoto ◽

J E Endo ◽

K S Shinmura ◽

M S Sano ◽

K F Fukuda

Keyword(s):

Endoplasmic Reticulum ◽

Fatty Acid Composition ◽

Fatty Acid ◽

Er Stress ◽

Diastolic Dysfunction ◽

Saturated Fatty Acid ◽

Diastolic Pressure ◽

Membrane Fatty Acid ◽

Membrane Fatty Acid Composition ◽

Scd1 Expression

Abstract Background Obesity-induced lipotoxicity causes cardiac dysfunction in our modern lifestyle. Previously, we have shown that an increase in cardiomyocyte membrane saturated fatty acid (SFA)/ monounsaturated FA (MUFA) ratio mediates endoplasmic reticulum (ER) stress, which was implicated in the pathogenesis of SFA-induced cardiomyopathy. Furthermore, SFA supressed Sirt1/ stearoyl-CoA desaturase-1 (SCD1, converting enzyme from SFA to MUFA) signaling, which further worsened the membrane SFA/MUFA ratio. Purpose To evaluate the effectiveness of targeting membrane fatty acid composition by MUFA. Methods and results In wild-type mice, 16-weeks SFA-rich high lard diet feeding (HLD) caused activation of PPARα signaling and the accumulation of toxic lipid intermediates (diacylglycerol and ceramide) in the heart to the same extent as a MUFA-rich high olive oil diet feeding (HOD). However, only the HLD impaired Sirt1 activity, SCD1 expression, diastolic function (increased left ventricular end-diastolic pressure (LVEDP) and end-diastolic pressure-volume relationship (EDPVR)), and cardiac remodeling (hypertrophy and fibrosis). Lipidome analysis showed that HLD-induced diastolic dysfunction coincided with an increase in membrane SFA/MUFA ratio and ER stress induction. 8-weeks HOD after 8-weeks HLD (HOD switch) showed the same degree of obesity and PPARα activation with 16-weeks HLD. By contrast, HOD switched heart were less severe Sirt1/SCD1 signaling dysregulation, increased in membrane SFA/MUFA ratio, ER stress, and cardiomyopathy (hypertrophy, fibrosis, and diastolic dysfunction) compared to 16-weeks HLD. Moreover, in cardiomyocyte-specific Sirt1 knockout mice, HOD switched heart also showed less severe increase in membrane SFA/MUFA ratio, ER stress, and cardiomyopathy compared to 16-weeks HLD although decreased SCD1 expression was not changed. Conclusions We demonstrated that MUFA-rich diet counteracted SFA-induced Sirt1/SCD1 signaling dysregulation and prevented SFA-induced increase in membrane SFA/MUFA ratio. Hence, MUFA-rich diet antagonized SFA-induced ER stress and cardiomyopathy even if Sirt1 deactivated heart (e.g., aged heart). Targeting the cardiomyocyte membrane SFA/MUFA ratio by MUFA might have a new therapeutic potential for SFA-induced cardiomyopathy. FUNDunding Acknowledgement Type of funding sources: Public Institution(s). Main funding source(s): JSPS KAKENHI

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Draft genome sequence of the silver pomfret fish, Pampus argenteus

Genome ◽

10.1139/gen-2015-0056 ◽

2016 ◽

Vol 59 (1) ◽

pp. 51-58 ◽

Cited By ~ 7

Author(s):

Sabah AlMomin ◽

Vinod Kumar ◽

Sami Al-Amad ◽

Mohsen Al-Hussaini ◽

Talal Dashti ◽

...

Keyword(s):

Genome Sequence ◽

Management Strategies ◽

Draft Genome ◽

Gene Clusters ◽

Draft Genome Sequence ◽

Pampus Argenteus ◽

Genomic Studies ◽

Next Generation Sequencing Ngs ◽

Generation Sequencing ◽

Silver Pomfret

Silver pomfret, Pampus argenteus, is a fish species from coastal waters. Despite its high commercial value, this edible fish has not been sequenced. Hence, its genetic and genomic studies have been limited. We report the first draft genome sequence of the silver pomfret obtained using a Next Generation Sequencing (NGS) technology. We assembled 38.7 Gb of nucleotides into scaffolds of 350 Mb with N50 of about 1.5 kb, using high quality paired end reads. These scaffolds represent 63.7% of the estimated silver pomfret genome length. The newly sequenced and assembled genome has 11.06% repetitive DNA regions, and this percentage is comparable to that of the tilapia genome. The genome analysis predicted 16 322 genes. About 91% of these genes showed homology with known proteins. Many gene clusters were annotated to protein and fatty-acid metabolism pathways that may be important in the context of the meat texture and immune system developmental processes. The reference genome can pave the way for the identification of many other genomic features that could improve breeding and population-management strategies, and it can also help characterize the genetic diversity of P. argenteus.

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