scholarly journals A set of isomeric episomal plasmids for systematic examination of mitotic stability inSaccharomyces cerevisiae

Yeast ◽  
2017 ◽  
Vol 34 (6) ◽  
pp. 267-275 ◽  
Author(s):  
Ruben Hohnholz ◽  
Kim Julia Pohlmann ◽  
Tilman Achstetter
Keyword(s):  
Mitotic Stability ◽  
Systematic Examination

Feeling Bad and Doing Good

2020 ◽  
Vol 51 (1) ◽  
pp. 35-49 ◽  
Author(s):  
Shoko Watanabe ◽  
Sean M. Laurent
Keyword(s):  
Third Party ◽  
Personal Involvement ◽  
Systematic Examination

Abstract. Previous forgiveness research has mostly focused on victims’ forgiveness of transgressors, and offenders’ post-transgression efforts intended to promote victim forgiveness have been collectively branded as apology. However, decisions concerning forgiveness frequently occur outside of dyadic contexts, and the unique roles of repentance and atonement in determining forgivability of offenders, despite their preeminence in theology and law, have received little empirical attention. Across five experiments ( N = 938), we show that repentance and atonement independently influence third-party perception of forgivability for a variety of harms, even in disinterested contexts. Our findings provide a systematic examination of decisions about forgivability disentangled from direct personal involvement, demonstrating that components of apology known to facilitate forgiveness in victims also increase perceived forgivability from unharmed observers.

Modeling of dynamic mosaicism on ring chromosomes in human fibroblasts and IPSCS

2020 ◽  
pp. 12-13
Author(s):  
Т.В. Никитина ◽  
А.А. Кашеварова ◽  
М.М. Гридина ◽  
А.А. Хабарова ◽  
А.Г. Мензоров ◽  
...  
Keyword(s):  
Genetic Structure ◽  
Human Fibroblasts ◽  
Cellular Heterogeneity ◽  
Mitotic Stability ◽  
Factors Affecting ◽  
Ring Chromosomes ◽  
Cell Clones ◽  
Mitotic Instability ◽  
Mitotic Behavior

Митотическая нестабильность кольцевых хромосом может приводить к появлению клеточных клонов с различной генетической структурой. В качестве модели нестабильности кольцевых хромосом в митозе мы использовали фибробласты от пациентов с r(8), r(13), r(18) и r(22) и полученные из них индуцированные плюрипотентные стволовые клетки (ИПСК). Линии ИПСК с r(22) имели относительно стабильный кариотип на протяжении десятков (до 60) пассажей и сохраняли неизменную структуру кольцевой хромосомы. Кариотип линий ИПСК с r(8) и r(18) на ранних пассажах стабильный, планируется его изучение на поздних пассажах. Наибольшее разнообразие кариотипа выявлено в линиях ИПСК с r(13), в которых наблюдали различные перестройки и выраженную клеточную гетерогенность. Определение факторов, влияющих на митотическую стабильность кольцевых хромосом, может иметь значение для консультирования пациентов. Mitotic instability of ring chromosomes can lead to the appearance of cell clones with different genetic structure. IPSCs from fibroblasts of patients with r(8), r(13), r(18), and r(22) were used as a model of ring chromosomes mitotic behavior. Karyotypes of iPSC lines with r(8) and r(18) have so far been evaluated only in the early passages, lines with r(22) have maintained a relatively stable karyotype up to 60 passages. The occurrence of rearrangements and cellular heterogeneity was found characteristic for r(13) iPSCs. The determination of factors affecting the ring chromosomes mitotic stability would be beneficial for the patient’s prognosis.

Temporal Brokering: A Measure of Brokerage as a Behavioral Process

2021 ◽  
pp. 109442812110029
Author(s):  
Eric Quintane ◽  
Martin Wood ◽  
John Dunn ◽  
Lucia Falzon
Keyword(s):  
Temporal Dynamics ◽  
Predictive Ability ◽  
Temporal Information ◽  
Behavioral Measure ◽  
Organizational Networks ◽  
Message Boards ◽  
Behavioral Process ◽  
Systematic Examination ◽  
The Way

Extant research in organizational networks has provided critical insights into understanding the benefits of occupying a brokerage position. More recently, researchers have moved beyond the brokerage position to consider the brokering processes (arbitration and collaboration) brokers engage in and their implications for performance. However, brokering processes are typically measured using scales that reflect individuals’ orientation toward engaging in a behavior, rather than the behavior itself. In this article, we propose a measure that captures the behavioral process of brokering. The measure indicates the extent to which actors engage in arbitration versus collaboration based on sequences of time stamped relational events, such as emails, message boards, and recordings of meetings. We demonstrate the validity of our measure as well as its predictive ability. By leveraging the temporal information inherent in sequences of relational events, our behavioral measure of brokering creates opportunities for researchers to explore the dynamics of brokerage and their impact on individuals, and also paves the way for a systematic examination of the temporal dynamics of networks.

Variación y cambio en la formalización de la interrogación retórica en la historia del español

2020 ◽  
Vol 136 (4) ◽  
pp. 1085-1113
Author(s):  
Andrés Enrique-Arias
Keyword(s):  
Biblical Hebrew ◽  
Systematic Examination ◽  
History Of

AbstractThis paper aims to investigate the specific mechanisms that contribute to formalizing the rhetorical interpretation of interrogative sentences throughout the history of Spanish. To this end, data from a corpus of biblical translations from the 13th to the 20th centuries are analyzed. As Biblical Hebrew and Latin use explicit elements to signal direct polar questions, it is relatively easy to locate a large number of passages with interrogative sentences, and then, examine the distribution of the translation equivalents used in the Spanish versions. The interrogative particles of Hebrew and Latin thus articulate a grammaticalized procedural meaning for which Spanish does not have an equivalent explicit marker. The systematic examination of the strategies that Spanish translators use to adapt these structures to their vernacular is therefore a way of accessing the intuitions of speakers from different periods in the history of Spanish in regard to the formulation of the rhetorical interrogative.

scholarly journals Chiming In: A Computer-Assisted Analysis of Popular Musicians’ Political Engagement on Twitter

2021 ◽  
Vol 7 (2) ◽  
pp. 205630512110190
Author(s):  
Josephine Lukito ◽  
Luis Loya ◽  
Carlos Dávalos ◽  
Jianing Li ◽  
Chau Tong ◽  
...  
Keyword(s):  
Case Studies ◽  
Hip Hop ◽  
Political Engagement ◽  
Future Research ◽  
Computer Assisted ◽  
Christian Music ◽  
Political Issues ◽  
Qualitative Case Studies ◽  
Systematic Examination ◽  
Assisted Analysis

While music as an artistic form is well studied, the individuals behind the art receive relatively less attention. In this article, we provide evidence of celebrity advocacy with a systematic examination of musicians’ political engagement on Twitter. This study estimates the extent to which musicians use Twitter for political purposes, with particular attention to whether such engagement varies across music genres. Through a computational-assisted analysis of 2,286,434 tweets, we group 881 musicians into three categories of political engagement on Twitter: not engaged (comprising the majority of artists), circumstantial engagement, and active political engagement. We examine the latter categories in detail with two qualitative case studies. The findings indicate that musicians from different genres have distinct patterns of political engagement. The Christian music genre shows the most engagement as a whole, especially in philanthropy. On the contrary, the most active accounts are rock and hip-hop artists, some of whom discuss political issues and call for mobilization. We conclude with suggestions for future research.

Micronuclear DNA sequences from Tetrahymena do not confer mitotic stability on ARS plasmids in Saccharomyces

Genome ◽  
1988 ◽  
Vol 30 (5) ◽  
pp. 690-696 ◽  
Author(s):  
Wendy H. Horsfall ◽  
Ronald E. Pearlman
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Dna Sequences ◽  
Copy Number ◽  
Tetrahymena Thermophila ◽  
Assay System ◽  
Mitotic Stability ◽  
Yeast Saccharomyces Cerevisiae ◽  
Genomic Libraries ◽  
Ade2 Gene

Genomic libraries containing micronuclear DNA sequences from Tetrahymena thermophila have been constructed in a vector containing ARS1, SUP11, and ura3 sequences from the yeast Saccharomyces cerevisiae. When transformed into a strain of S. cerevisiae carrying a suppressible ochre mutation in the ade2 gene, viable transformants are obtained only if the transforming plasmid is maintained at a copy number of one or two per cell. Mitotic segregation of the plasmid is easily assessed in a colour assay of transformants. Using this assay system, we showed that micronuclear DNA from Tetrahymena does not contain sequences that confer mitotic stability on yeast ARS-containing plasmids; i.e., sequences that function analogously to yeast centromere sequences. One transformant was analyzed that carries Tetrahymena sequences that maintain the copy number of the ARS plasmid at one or two per cell. However, these sequences do not confer mitotic stability on the transformants and they confer a phenotype in this assay similar to that of the REP3 gene of the yeast 2 μm plasmid.Key words: mitotic stability, centromere, Tetrahymena, Saccharomyces.

Shoulder examination: a systematic approach

2021 ◽  
pp. 1-15
Author(s):  
Akib M Khan ◽  
Angelos Assiotis ◽  
Mazin S Ibrahim ◽  
Andrew R Sankey
Keyword(s):  
Rotator Cuff ◽  
Systematic Approach ◽  
Healthcare Professionals ◽  
Full Range ◽  
Range Of Movement ◽  
Systematic Examination ◽  
Complex Joint ◽  
Different Levels ◽  
Shoulder Examination

The shoulder is a complex joint with static and dynamic stabilising structures working synchronously. These allow a full range of movement while preserving stability of the joint. Patients may present with pain, stiffness, weakness, deformity or instability. The authors suggest a systematic examination sequence to ensure that important pathology is not overlooked. Adopting this approach allows common pathologies, including tears of the rotator cuff, impingement and tendinopathy, to be easily identified. This shoulder examination sequence may be used by all healthcare professionals and can also act as a revision aid for those undergoing exams in this field, at different levels of training.

Sign in / Sign up

Export Citation Format

Share Document