Differential Extraction of Sperm and Epithelial Cell DNA

It is well known that epithelial hyperplasia (benign hypertrophy) is common in the aging prostate of dogs and man. In contrast, little evidence is available for abnormal epithelial cell growth in seminal vesicles of aging animals. Recently, enlarged seminal vesicles were reported in senescent mice, however, that enlargement resulted from increased storage of secretion in the lumen and occurred concomitant to epithelial hypoplasia in that species.The present study is concerned with electron microscopic observations of changes occurring in the pseudostratified epithelium of the seminal vescles of aging rats. Special attention is given to certain non-epithelial cells which have entered the epithelial layer.

Download Full-text

Intranuclear Crystals in Regenerating Canine Kidneys

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100072502 ◽

1973 ◽

Vol 31 ◽

pp. 412-413

Author(s):

D.G. Osborne ◽

L.J. McCormack ◽

M.O. Magnusson ◽

W.S. Kiser

Keyword(s):

Epithelial Cell ◽

Epithelial Cells ◽

Tubular Epithelial Cell ◽

Tubular Epithelial Cells ◽

Cell Nuclei ◽

Crystalline Structures ◽

Small Crystals ◽

Membrane Bound

During a project in which regenerative changes were studied in autotransplanted canine kidneys, intranuclear crystals were seen in a small number of tubular epithelial cells. These crystalline structures were seen in the control specimens and also in regenerating specimens; the main differences being in size and number of them. The control specimens showed a few tubular epithelial cell nuclei almost completely occupied by large crystals that were not membrane bound. Subsequent follow-up biopsies of the same kidneys contained similar intranuclear crystals but of a much smaller size. Some of these nuclei contained several small crystals. The small crystals occurred at one week following transplantation and were seen even four weeks following transplantation. As time passed, the small crystals appeared to fuse to form larger crystals.

Download Full-text

An Established Epithelial Cell Line (NPC/HK1) from a Nasopharyngeal Carcinoma - II. A Sem Study

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100053784 ◽

1982 ◽

Vol 40 ◽

pp. 224-225

Author(s):

Li C.L. ◽

Chew E.C. ◽

Huang D.P. ◽

Ho H.C. ◽

Mak L.S. ◽

...

Keyword(s):

Nasopharyngeal Carcinoma ◽

Epithelial Cell ◽

Surface Morphology ◽

Cell Line ◽

Epithelial Cell Line ◽

Present Communication ◽

Cells In Culture ◽

Sem Study ◽

Well Differentiated

An epithelial cell line, NPC/HK1, has recently been successfully established from a nasopharyngeal carcinoma of the moderately to well differentiated squamous type. The present communication reports on the surface morphology of the NPC/HK1 cells in culture.

Download Full-text

Ultrastructural Changes of Smoooth Endoplasmic Reticulum in the Retinal Pigment Epithelium by Choline Chloride

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100093547 ◽

1972 ◽

Vol 30 ◽

pp. 58-59

Author(s):

Kazushige Hirosawa ◽

Eichi Yamada

Keyword(s):

Endoplasmic Reticulum ◽

Epithelial Cell ◽

Smooth Endoplasmic Reticulum ◽

Pigment Epithelium ◽

Choline Chloride ◽

Retinal Pigment ◽

Ultrastructural Changes ◽

Lower Vertebrate ◽

Visual Cells ◽

Pigment Epithelial

The pigment epithelium is located between the choriocapillary and the visual cells. The pigment epithelial cell is characterized by a large amount of the smooth endoplasmic reticulum (SER) in its cytoplasm. In addition, the pigment epithelial cell of some lower vertebrate has myeloid body as a specialized form of the SER. Generally, SER is supposed to work in the lipid metabolism. However, the functions of abundant SER and myeloid body in the pigment epithelial cell are still in question. This paper reports an attempt, to depict the functions of these organelles in the frog retina by administering one of phospholipid precursors.

Download Full-text

Ca++-ATPase activity in the hepatopancreas cells of Oniscus asellus

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100124501 ◽

1992 ◽

Vol 50 (1) ◽

pp. 820-821

Author(s):

G.M. Vernon ◽

A. Surace ◽

R. Witkus

Keyword(s):

B Cells ◽

Epithelial Cell ◽

Atpase Activity ◽

Calcium Transport ◽

Carcinus Maenas ◽

Cell Types ◽

Molt Cycle ◽

Copper And Zinc

The hepatopancreas consists of a pair of bilobed tubules comprised of two epithelial cell types. S cells are absorptive and accumulate metals such as copper and zinc. Ca++ concentrations vary between the S and B cells and during the molt cycle. Roer and Dillaman implicated Ca++-ATPase in calcium transport during molting in Carcinus maenas. This study was undertaken to compare the localization of Ca++-ATPase activity in the S and B cells during intermolt.

Download Full-text

The Effects of Estradiol on Chronically Damaged Human Fallopian Tubes: A SEM Study

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100119934 ◽

1985 ◽

Vol 43 ◽

pp. 648-649

Author(s):

A. Toledo ◽

G. Stoelk ◽

M. Yussman ◽

R.P. Apkarian

Keyword(s):

Menstrual Cycle ◽

Epithelial Cell ◽

Secretory Cell ◽

Estrogen Therapy ◽

Fallopian Tubes ◽

Cell Surfaces ◽

Proliferative Phase ◽

Cyclic Patterns ◽

Sem Study ◽

The U.S

Today it is estimated that one of every three women in the U.S. will have problems achieving pregnancy. 20-30% of these women will have some form of oviductal problems as the etiology of their infertility. Chronically damaged oviducts present problems with loss of both ciliary and microvillar epithelial cell surfaces. Estradiol is known to influence cyclic patterns in secretory cell microvilli and tubal ciliogenesis, The purpose of this study was to assess whether estrogen therapy could stimulate ciliogenesis in chronically damaged human fallopian tubes.Tissues from large hydrosalpinges were obtained from six women undergoing tuboplastic repair while in the early proliferative phase of fheir menstrual cycle. In each case the damaged tissue was rinsed in heparinized Ringers-lactate and quartered.

Download Full-text