The Filtration Concept of the Blood-CSF-Barrier as Basis for the Differentiation of CSF Proteins

1995 ◽  
pp. 209-217 ◽  
Author(s):  
K. Felgenhauer
Keyword(s):  
Csf Proteins

scholarly journals Comparison of plasma and cerebrospinal fluid proteomes identifies gene products guiding adult neurogenesis and neural differentiation in birds

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Eleni Voukali ◽  
Nithya Kuttiyarthu Veetil ◽  
Pavel Němec ◽  
Pavel Stopka ◽  
Michal Vinkler
Keyword(s):  
Cerebrospinal Fluid ◽  
Adult Neurogenesis ◽  
Neural Development ◽  
Neural Differentiation ◽  
Csf Proteins ◽  
Nymphicus Hollandicus ◽  
Liquid Chromatography Tandem Mass ◽  
Genome Information ◽  
Insight Into ◽  
Brain Homeostasis

AbstractCerebrospinal fluid (CSF) proteins regulate neurogenesis, brain homeostasis and participate in signalling during neuroinflammation. Even though birds represent valuable models for constitutive adult neurogenesis, current proteomic studies of the avian CSF are limited to chicken embryos. Here we use liquid chromatography–tandem mass spectrometry (nLC-MS/MS) to explore the proteomic composition of CSF and plasma in adult chickens (Gallus gallus) and evolutionarily derived parrots: budgerigar (Melopsittacus undulatus) and cockatiel (Nymphicus hollandicus). Because cockatiel lacks a complete genome information, we compared the cross-species protein identifications using the reference proteomes of three model avian species: chicken, budgerigar and zebra finch (Taeniopygia guttata) and found the highest identification rates when mapping against the phylogenetically closest species, the budgerigar. In total, we identified 483, 641 and 458 unique proteins consistently represented in the CSF and plasma of all chicken, budgerigar and cockatiel conspecifics, respectively. Comparative pathways analyses of CSF and blood plasma then indicated clusters of proteins involved in neurogenesis, neural development and neural differentiation overrepresented in CSF in each species. This study provides the first insight into the proteomics of adult avian CSF and plasma and brings novel evidence supporting the adult neurogenesis in birds.

scholarly journals Comprehensive Phenotyping in Multiple Sclerosis: Discovery Based Proteomics and the Current Understanding of Putative Biomarkers

2006 ◽  
Vol 22 (4) ◽  
pp. 213-225 ◽  
Author(s):  
Kevin C. O’Connor ◽  
Sushmita Mimi Roy ◽  
Christopher H. Becker ◽  
David A. Hafler ◽  
Aaron B. Kantor
Keyword(s):  
Magnetic Resonance Imaging ◽  
Multiple Sclerosis ◽  
Accurate Diagnosis ◽  
Disease Monitoring ◽  
Single Test ◽  
Resonance Imaging ◽  
Control Groups ◽  
Csf Proteins ◽  
Magnetic Resonance Imaging Mri ◽  
And Control

Currently, there is no single test for multiple sclerosis (MS). Diagnosis is confirmed through clinical evaluation, abnormalities revealed by magnetic resonance imaging (MRI), and analysis of cerebrospinal fluid (CSF) chemistry. The early and accurate diagnosis of the disease, monitoring of progression, and gauging of therapeutic intervention are important but elusive elements of patient care. Moreover, a deeper understanding of the disease pathology is needed, including discovery of accurate biomarkers for MS. Herein we review putative biomarkers of MS relating to neurodegeneration and contributions to neuropathology, with particular focus on autoimmunity. In addition, novel assessments of biomarkers not driven by hypotheses are discussed, featuring our application of advanced proteomics and metabolomics for comprehensive phenotyping of CSF and blood. This strategy allows comparison of component expression levels in CSF and serum between MS and control groups. Examination of these preliminary data suggests that several CSF proteins in MS are differentially expressed, and thus, represent putative biomarkers deserving of further evaluation.

scholarly journals Serum and cerebrospinal fluid host proteins indicate stroke in children with tuberculous meningitis

PLoS ONE ◽  
2021 ◽  
Vol 16 (4) ◽  
pp. e0250944
Author(s):  
Charles M. Manyelo ◽  
Novel N. Chegou ◽  
James A. Seddon ◽  
Candice I. Snyders ◽  
Hygon Mutavhatsindi ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
Tuberculous Meningitis ◽  
Multiple Testing ◽  
Serum Proteins ◽  
Lipocalin 2 ◽  
Host Proteins ◽  
Serum Samples ◽  
Csf Proteins ◽  
Study Participants

Introduction Stroke is a common complication in children with tuberculous meningitis (TBM). Host proteins may give us insight into the mechanisms of stroke in TBM and serve as biomarkers for detection of stroke, however, they have not been widely explored. In this study, we compared the concentrations of cerebrospinal fluid (CSF) and serum proteins between children who had TBM-related stroke and children with TBM without stroke. Methods We collected CSF and serum from 47 children consecutively admitted to the Tygerberg Academic Hospital in Cape Town, South Africa between November 2016, and November 2017, on suspicion of having TBM. A multiplex platform was used to measure the concentrations of 69 host proteins in CSF and serum from all study participants. Results After classification of study participants, 23 (48.9%) out of the 47 study participants were diagnosed with TBM, of which 14 (60.9%) demonstrated radiological arterial ischemic infarction. The levels of lipocalin-2, sRAGE, IP-10/ CXCL10, sVCAM-1, MMP-1, and PDGF-AA in CSF samples and the levels of D-dimer, ADAMTS13, SAA, ferritin, MCP-1/ CCL2, GDF-15 and IL-13 in serum samples were statistically different between children who had TBM-related stroke and children with TBM without stroke. After correcting for multiple testing, only the levels of sVCAM-1, MMP-1, sRAGE, and IP-10/ CXCL10 in CSF were statistically different between the two groups. CSF and serum protein biosignatures indicated stroke in children diagnosed with TBM with up to 100% sensitivity and 88.9% specificity. Conclusion Serum and CSF proteins may serve as biomarkers for identifying individuals with stroke amongst children diagnosed with TBM at admission and may guide us to understand the biology of stroke in TBM. This was a pilot study, and thus further investigations in larger studies are needed.

Brain and CSF Proteins in Creutzfeldt-Jakob Disease

1980 ◽  
Vol 19 (2) ◽  
pp. 85-90 ◽  
Author(s):  
Jan-Edvin Olsson
Keyword(s):  
Csf Proteins ◽  
Jakob Disease

scholarly journals Diagnostic accuracy of urinary reagent strip to determine cerebrospinal fluid chemistry and cellularity

2013 ◽  
Vol 04 (02) ◽  
pp. 140-145 ◽  
Author(s):  
Deepti Joshi ◽  
Keerthi Kundana ◽  
Apurva Puranik ◽  
Rajnish Joshi
Keyword(s):  
Cerebrospinal Fluid ◽  
Diagnostic Accuracy ◽  
Area Under The Curve ◽  
Test Strip ◽  
Index Test ◽  
Csf Proteins ◽  
Csf Cells ◽  
Overall Performance ◽  
Index Tests ◽  
Csf Examination

ABSTRACT Background: The gold standard for diagnosis of meningitis depends on cerebrospinal fluid (CSF) examination by microscopy, biochemistry, and culture, which require an experienced microscopist and laboratory support. We conducted this study to determine if urinary reagent strip is useful to make a semi‑quantitative assessment of protein, glucose, and presence of leukocyte esterase in CSF. Materials and Methods: All consecutive CSF samples were evaluated in a blinded fashion. CSF was tested using Combur‑10 urinary reagent strip as an index test, and CSF microscopy and biochemistry as reference standards. Combur‑10 (Boehringer Mannheim) is a urinary reagent strip used to estimate ten parameters including protein, glucose, and leukocytes. We estimated diagnostic accuracy of each index test using corresponding cut‑off levels (glucose 1 + vs. CSF glucose >50 mg/dL; protein 1 + and 2 + vs. CSF protein >30 mg/dL and >100 mg/dL; leukocyte esterase positivity vs. >10 granulocytes in CSF sample). We constructed receiver operating curves (ROC) to evaluate overall performance of index tests and estimated area under the curve (AUC). Results: CSF samples of 75 patients were included in the study. All the three indicator tests (CSF cells, protein, and glucose) were normal in 17 (22.6%) samples. Of the three tests, diagnostic accuracy of protein estimation (1 + or more on reagent strip) was best for detection of CSF proteins greater than 30 mg/dL [sensitivity 98.1% (95% CI 90.1-100%); specificity 57.1% (95% CI 34-78.2%)], with AUC of 0.97. Sensitivity and specificity for 2 + on reagent strip and CSF protein > 100 mg/dL were 92.6% (95% CI 75.1-99.1) and 87.5% (95% CI 74.8-95.3), respectively, with AUC of 0.96 (95% CI 0.92-1.01). Leukocyte esterase positivity by test strip had a sensitivity of 85.2 (95% CI 66.3-95.8%) and specificity of 89.6 (95% CI 77.3-96.5%) for detection of CSF granulocytes of more than 10/mm3. Conclusion: Existing urinary reagent strips can be used to diagnose meningitis in low‑resource settings.

Utility of high resolution accurate mass spectrometry (HRMS) in the mass isotopomer distribution analysis (MIDA) of CSF proteins modified by stable isotope labeling in mammals (SILAM) methodology applied to neurodegenerative diseases

2017 ◽  
Vol 9 (23) ◽  
pp. 3477-3484
Author(s):  
Joseph L. Cantone ◽  
Craig Polson ◽  
Cong Wei ◽  
Valerie Guss ◽  
Michael K. Ahlijanian ◽  
...  
Keyword(s):  
Stable Isotope ◽  
Isotope Labeling ◽  
Molecular Level ◽  
Disease Diagnosis ◽  
Stable Isotope Labeling ◽  
Distribution Analysis ◽  
Mass Isotopomer Distribution Analysis ◽  
Csf Proteins ◽  
Accurate Mass Spectrometry

Stable isotope labeling of proteins affords indicators at the molecular level, specifically biomarkers, which may providein vivodata on disease diagnosis, progression, and treatment.

Individual cerebrospinal fluid (CSF) proteins in the evaluation of increased CSF total protein

1972 ◽  
Vol 203 (2) ◽  
pp. 119-132 ◽  
Author(s):  
H. Link ◽  
O. Zettervall ◽  
G. Blennow
Keyword(s):  
Cerebrospinal Fluid ◽  
Total Protein ◽  
Csf Proteins
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