Isolation and Propagation of Rat Peritoneal Mesothelial Cells

2015 ◽  
pp. 25-34 ◽  
Author(s):  
Qin Zhou ◽  
Xueqing Yu
Keyword(s):  
Mesothelial Cells ◽  
Peritoneal Mesothelial Cells

scholarly journals Senescent Peritoneal Mesothelial Cells Promote Ovarian Cancer Cell Adhesion

2009 ◽  
Vol 174 (4) ◽  
pp. 1230-1240 ◽  
Author(s):  
Krzysztof Ksiazek ◽  
Justyna Mikula-Pietrasik ◽  
Katarzyna Korybalska ◽  
Grzegorz Dworacki ◽  
Achim Jörres ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Cell Adhesion ◽  
Cancer Cell ◽  
Ovarian Cancer Cell ◽  
Mesothelial Cells ◽  
Peritoneal Mesothelial Cells ◽  
Cancer Cell Adhesion

scholarly journals High glucose levels inhibit focal adhesion kinase-mediated wound healing of rat peritoneal mesothelial cells

2003 ◽  
Vol 63 (2) ◽  
pp. 722-731 ◽  
Author(s):  
Masahito Tamura ◽  
Akihiko Osajima ◽  
Shingo Nakayamada ◽  
Hirofumi Anai ◽  
Narutoshi Kabashima ◽  
...  
Keyword(s):  
Wound Healing ◽  
Focal Adhesion Kinase ◽  
Focal Adhesion ◽  
High Glucose ◽  
Mesothelial Cells ◽  
Peritoneal Mesothelial Cells ◽  
Glucose Levels

Mesothelial Cells

2007 ◽  
Vol 27 (2_suppl) ◽  
pp. 110-115 ◽  
Author(s):  
Susan Yung ◽  
Chan Tak Mao
Keyword(s):  
Mesothelial Cell ◽  
In Vitro Models ◽  
Mesothelial Cells ◽  
Dynamic Membrane ◽  
Peritoneal Mesothelial Cells ◽  
Immunohistochemical Markers ◽  
Vitro Model ◽  
And Function

♦ Background The introduction of peritoneal dialysis (PD) as a modality of renal replacement therapy has provoked much interest in the biology of the peritoneal mesothelial cell. Mesothelial cells isolated from omental tissue have immunohistochemical markers that are identical to those of mesothelial stem cells, and omental mesothelial cells can be cultivated in vitro to study changes to their biologic functions in the setting of PD. ♦ Method The present article describes the structure and function of mesothelial cells in the normal peritoneum and details the morphologic changes that occur after the introduction of PD. Furthermore, this article reviews the literature of mesothelial cell culture and the limitations of in vitro studies. ♦ Results The mesothelium is now considered to be a dynamic membrane that plays a pivotal role in the homeostasis of the peritoneal cavity, contributing to the control of fluid and solute transport, inflammation, and wound healing. These functional properties of the mesothelium are compromised in the setting of PD. Cultures of peritoneal mesothelial cells from omental tissue provide a relevant in vitro model that allows researchers to assess specific molecular pathways of disease in a distinct population of cells. Structural and functional attributes of mesothelial cells are discussed in relation to long-term culture, proliferation potential, age of tissue donor, use of human or animal in vitro models, and how the foregoing factors may influence in vitro data. ♦ Conclusions The ability to propagate mesothelial cells in culture has resulted, over the past two decades, in an explosion of mesothelial cell research pertaining to PD and peritoneal disorders. Independent researchers have highlighted the potential use of mesothelial cells as targets for gene therapy or transplantation in the search to provide therapeutic strategies for the preservation of the mesothelium during chemical or bacterial injury.

Modulation of Fibrinolytic System Components in Mesothelial Cells by Hyaluronan

2003 ◽  
Vol 23 (3) ◽  
pp. 222-227 ◽  
Author(s):  
Thomas Sitter ◽  
Matthias Sauter ◽  
Bettina Haslinger
Keyword(s):  
Mrna Expression ◽  
Plasminogen Activator ◽  
Positive Impact ◽  
Density Lipoprotein ◽  
Mesothelial Cells ◽  
Tissue Type ◽  
Dialysis Fluid ◽  
Peritoneal Mesothelial Cells ◽  
The Impact ◽  
Pai 1

← Objective Hyaluronan (HA) is an important extracellular matrix component and is involved in fluid homeostasis, tissue repair, and response to infections. Previous studies have shown that supplementation of dialysis fluid with high molecular weight HA may have a positive impact on peritoneal solute and fluid transport characteristics. In the present study, we investigated the impact of HA on the synthesis of tissue-type plasminogen activator (t-PA) and its inhibitor, plasminogen activator inhibitor type 1 (PAI-1) in cultured human peritoneal mesothelial cells (MC). ← Methods Cultured human peritoneal MC isolated from omental tissue were used for the experiments. Concentrations of t-PA and PAI-1 antigens were measured in conditioned media of confluent MC using ELISA. Northern blot analysis was performed to investigate mRNA expression of t-PA, PAI-1, and low-density lipoprotein receptor-related protein. ← Results Hyaluronan in a concentration as suggested for supplementation of dialysis fluid (10 mg/dL) did not have a significant impact on the synthesis of t-PA or PAI-1 in human MC. However, incubation of MC with higher concentrations of HA (30 – 1000 mg/dL) resulted in a concentration- and time- (8 – 48 hours) dependent decrease in t-PA antigen release and mRNA expression. In contrast, PAI-1 antigen secretion was distinctly but not significantly increased in the presence of HA. ← Conclusion The expression of t-PA and PAI-1 in MC was not affected by low concentrations of HA. Therefore, it is reasonable to assume that supplementation of dialysis fluid with HA (10 mg/dL) will not decrease mesothelial fibrinolytic activity. Only high concentrations (> 50 mg/dL) may disturb the balance between intraperitoneal generation and degradation of fibrin by decreasing t-PA synthesis.

Influence of monocyte-like cells on the fibrinolytic activity of peritoneal mesothelial cells and the effect of sodium hyaluronate

2005 ◽  
Vol 84 ◽  
pp. 1072-1077 ◽  
Author(s):  
Cornelis J.J.M. Sikkink ◽  
Michel M.P.J. Reijnen ◽  
Peter Falk ◽  
Harry van Goor ◽  
Lena Holmdahl
Keyword(s):  
Fibrinolytic Activity ◽  
Sodium Hyaluronate ◽  
Mesothelial Cells ◽  
Peritoneal Mesothelial Cells
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